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The map label for this gene is prfC [H]

Identifier: 225686815

GI number: 225686815

Start: 1025749

End: 1027326

Strand: Direct

Name: prfC [H]

Synonym: BMEA_B1050

Alternate gene names: 225686815

Gene position: 1025749-1027326 (Clockwise)

Preceding gene: 225686813

Following gene: 225686819

Centisome position: 86.52

GC content: 58.75

Gene sequence:

>1578_bases
ATGCCCGCTGACCATCCAGTTACAAAGCGTCGCACATTCGCGATCATCGCGCACCCGGACGCCGGTAAAACCACGCTGAC
AGAAAAGCTGCTGCTGTTTGGCGGCGCTATCCAGCTTGCGGGTGAAGTGAAGGCCAAGAAAGACCGCATCCAGACCCGCT
CGGACTGGATGAACATCGAGCGCGACCGCGGCATTTCCGTCGTCACCTCCGTGATGACCTTTGAATACAAGGACTGCATC
TTTAATCTGCTCGACACGCCGGGCCACGAAGATTTTGCCGACGACACCTACCGCACGCTGACAGCGGTGGATAGCGCCGT
CATGGTCATCGACGCTGCCAAGGGTATCGAGCCACGCACGCTGAAACTTTTTGAAGTCTGCCGTATGCGTGACATTCCCA
TCGTCACCTTCGTCAACAAGATGGACCGCGAAGCACGCGATCCACTGGAAATCCTTGACGAGATCGAGGAAAAACTGGCG
CTCGACACAGCCCCCATCACCTGGCCGATCGGTTCCGGCAAGAGCTTCGCCGGCACTTTCGATCTGCACAACAATACGCT
GCGCCGCCAGGACAATGAAGAACAGCCGACCAGGGTGAGCGGCCCGGAAGAAGCCGCAAAGCTTCTGCCGGAAAACGAGC
GCGAAGCATGGCTGGAAGGCGTGGAGCTGGCGCGCGGCGTCTGCCGCCCCTTCGATCTTGCCTCTTTCCGTGAAGGCCAT
ATGACGCCGGTTTATTTCGGTTCGGCACTGAAAAATTACGGCGTGCGCGACCTGATCGAAGCTTTCTGCGATTTCGGCCC
AAGCCCACGCGACCAGCAGGCGGATGCGCGCATGGTGGCGGCAACCGAAGACAAGATGACCGGTTTCGTGTTCAAGATTC
AGGCCAATATGGACCCGAACCACCGCGACCGCATTGCCTTCCTGCGCGTCTGCTCGGGCAAGCTTTCGCGCGGCATGAAG
GCAAAACTGGTGCGCACCGGCAAACCGATGAGCCTCTCGGCACCGCAATTCTTCTTCGCCCGCTCACGCCAGATTGCGGA
TGAAGCCTTTGCGGGCGACGTGGTCGGCATTCCAATCCATGGCACGCTGCGCATCGGCGATACGCTGACCGACGGCGAGG
ATATCCTGTTCCGAGGCGTGCCGAATTTTGCGCCGGAAATCCTGCGCCGTGTGCGTCTTGACGACGCCATGAAGGCCAAG
AAGCTGCGCGAAGCCCTGCAACAGATGGCCGAGGAAGGCGTGGTGCAGCTTTTCGTGCCGGACGATGGTTCGCCCGCTAT
TGTAGGCGTCGTCGGCGCGCTTCAGATCGACGTTCTGACCGAACGCCTGAAGGTGGAATATTCACTGCCGGTCGGCTTTG
AAATGTCGCGCTTTTCAATCTGCCGGTGGATTTCCGCCGATAATCCGGCTGAACTCGATCGTTTCATCGCAGCCCATCGC
GCCGATATCGCGCATGATCTCGACAACGATCCCGTTTTTCTTGCCCAGAACGGTTTCTCGCTCAATTACGAGGCCGAACG
CTGGAAGGATATCCGCTTCGCCACCATCAAGGACTATCAGGTGCGTGACAAGGTTTAA

Upstream 100 bases:

>100_bases
GACTTGTTGAGATTCCGCCCAATGACTGCTAGATGCTGCGCAACATCTTCGCAGTGCGCTTTCACGCGCGGCCCAACATT
TCGCCATCACGAGTTTTTTC

Downstream 100 bases:

>100_bases
CCAAGAAGGCTGGCGGCGATTTTTTCCGCCAGTCTTTCAGCGACGTCTTCCTTGCTCATTTCCGGCCATTCCTCAATGCC
GGAATGGCTCAAGATACGCA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MPADHPVTKRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRIQTRSDWMNIERDRGISVVTSVMTFEYKDCI
FNLLDTPGHEDFADDTYRTLTAVDSAVMVIDAAKGIEPRTLKLFEVCRMRDIPIVTFVNKMDREARDPLEILDEIEEKLA
LDTAPITWPIGSGKSFAGTFDLHNNTLRRQDNEEQPTRVSGPEEAAKLLPENEREAWLEGVELARGVCRPFDLASFREGH
MTPVYFGSALKNYGVRDLIEAFCDFGPSPRDQQADARMVAATEDKMTGFVFKIQANMDPNHRDRIAFLRVCSGKLSRGMK
AKLVRTGKPMSLSAPQFFFARSRQIADEAFAGDVVGIPIHGTLRIGDTLTDGEDILFRGVPNFAPEILRRVRLDDAMKAK
KLREALQQMAEEGVVQLFVPDDGSPAIVGVVGALQIDVLTERLKVEYSLPVGFEMSRFSICRWISADNPAELDRFIAAHR
ADIAHDLDNDPVFLAQNGFSLNYEAERWKDIRFATIKDYQVRDKV

Sequences:

>Translated_525_residues
MPADHPVTKRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRIQTRSDWMNIERDRGISVVTSVMTFEYKDCI
FNLLDTPGHEDFADDTYRTLTAVDSAVMVIDAAKGIEPRTLKLFEVCRMRDIPIVTFVNKMDREARDPLEILDEIEEKLA
LDTAPITWPIGSGKSFAGTFDLHNNTLRRQDNEEQPTRVSGPEEAAKLLPENEREAWLEGVELARGVCRPFDLASFREGH
MTPVYFGSALKNYGVRDLIEAFCDFGPSPRDQQADARMVAATEDKMTGFVFKIQANMDPNHRDRIAFLRVCSGKLSRGMK
AKLVRTGKPMSLSAPQFFFARSRQIADEAFAGDVVGIPIHGTLRIGDTLTDGEDILFRGVPNFAPEILRRVRLDDAMKAK
KLREALQQMAEEGVVQLFVPDDGSPAIVGVVGALQIDVLTERLKVEYSLPVGFEMSRFSICRWISADNPAELDRFIAAHR
ADIAHDLDNDPVFLAQNGFSLNYEAERWKDIRFATIKDYQVRDKV
>Mature_524_residues
PADHPVTKRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRIQTRSDWMNIERDRGISVVTSVMTFEYKDCIF
NLLDTPGHEDFADDTYRTLTAVDSAVMVIDAAKGIEPRTLKLFEVCRMRDIPIVTFVNKMDREARDPLEILDEIEEKLAL
DTAPITWPIGSGKSFAGTFDLHNNTLRRQDNEEQPTRVSGPEEAAKLLPENEREAWLEGVELARGVCRPFDLASFREGHM
TPVYFGSALKNYGVRDLIEAFCDFGPSPRDQQADARMVAATEDKMTGFVFKIQANMDPNHRDRIAFLRVCSGKLSRGMKA
KLVRTGKPMSLSAPQFFFARSRQIADEAFAGDVVGIPIHGTLRIGDTLTDGEDILFRGVPNFAPEILRRVRLDDAMKAKK
LREALQQMAEEGVVQLFVPDDGSPAIVGVVGALQIDVLTERLKVEYSLPVGFEMSRFSICRWISADNPAELDRFIAAHRA
DIAHDLDNDPVFLAQNGFSLNYEAERWKDIRFATIKDYQVRDKV

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=483, Percent_Identity=26.7080745341615, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI19923640, Length=505, Percent_Identity=28.1188118811881, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI25306283, Length=341, Percent_Identity=31.9648093841642, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI25306287, Length=477, Percent_Identity=27.6729559748428, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI157426893, Length=156, Percent_Identity=33.974358974359, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=31.6546762589928, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI310132016, Length=115, Percent_Identity=33.0434782608696, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310110807, Length=115, Percent_Identity=33.0434782608696, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310123363, Length=115, Percent_Identity=33.0434782608696, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790835, Length=526, Percent_Identity=48.0988593155894, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1789738, Length=492, Percent_Identity=27.6422764227642, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1788922, Length=157, Percent_Identity=35.6687898089172, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=32.2147651006711, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=489, Percent_Identity=25.9713701431493, Blast_Score=162, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17556745, Length=480, Percent_Identity=25.625, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17557151, Length=139, Percent_Identity=33.8129496402878, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323098, Length=478, Percent_Identity=27.8242677824268, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6322359, Length=506, Percent_Identity=26.4822134387352, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6323320, Length=162, Percent_Identity=29.0123456790123, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324707, Length=154, Percent_Identity=31.8181818181818, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320593, Length=154, Percent_Identity=31.8181818181818, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24582462, Length=480, Percent_Identity=26.875, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI221458488, Length=394, Percent_Identity=30.9644670050761, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI78706572, Length=180, Percent_Identity=31.1111111111111, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=30.718954248366, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=30.718954248366, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=30.718954248366, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58926; Mature: 58795

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPADHPVTKRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRIQTRSDWMNIE
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCHHEECCHHHHHHHHHHHHHHHCCCC
RDRGISVVTSVMTFEYKDCIFNLLDTPGHEDFADDTYRTLTAVDSAVMVIDAAKGIEPRT
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCHH
LKLFEVCRMRDIPIVTFVNKMDREARDPLEILDEIEEKLALDTAPITWPIGSGKSFAGTF
HHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEE
DLHNNTLRRQDNEEQPTRVSGPEEAAKLLPENEREAWLEGVELARGVCRPFDLASFREGH
ECCCCHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCC
MTPVYFGSALKNYGVRDLIEAFCDFGPSPRDQQADARMVAATEDKMTGFVFKIQANMDPN
CCCEEHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCC
HRDRIAFLRVCSGKLSRGMKAKLVRTGKPMSLSAPQFFFARSRQIADEAFAGDVVGIPIH
CHHHHHHHHHHHHHHCCCCEEEHEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCE
GTLRIGDTLTDGEDILFRGVPNFAPEILRRVRLDDAMKAKKLREALQQMAEEGVVQLFVP
EEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
DDGSPAIVGVVGALQIDVLTERLKVEYSLPVGFEMSRFSICRWISADNPAELDRFIAAHR
CCCCCEEEEHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHH
ADIAHDLDNDPVFLAQNGFSLNYEAERWKDIRFATIKDYQVRDKV
HHHHHCCCCCCEEEEECCCEECCCHHHHCCEEEEEEECCCCCCCC
>Mature Secondary Structure 
PADHPVTKRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRIQTRSDWMNIE
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCHHEECCHHHHHHHHHHHHHHHCCCC
RDRGISVVTSVMTFEYKDCIFNLLDTPGHEDFADDTYRTLTAVDSAVMVIDAAKGIEPRT
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCHH
LKLFEVCRMRDIPIVTFVNKMDREARDPLEILDEIEEKLALDTAPITWPIGSGKSFAGTF
HHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEE
DLHNNTLRRQDNEEQPTRVSGPEEAAKLLPENEREAWLEGVELARGVCRPFDLASFREGH
ECCCCHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCC
MTPVYFGSALKNYGVRDLIEAFCDFGPSPRDQQADARMVAATEDKMTGFVFKIQANMDPN
CCCEEHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCC
HRDRIAFLRVCSGKLSRGMKAKLVRTGKPMSLSAPQFFFARSRQIADEAFAGDVVGIPIH
CHHHHHHHHHHHHHHCCCCEEEHEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCE
GTLRIGDTLTDGEDILFRGVPNFAPEILRRVRLDDAMKAKKLREALQQMAEEGVVQLFVP
EEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
DDGSPAIVGVVGALQIDVLTERLKVEYSLPVGFEMSRFSICRWISADNPAELDRFIAAHR
CCCCCEEEEHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHH
ADIAHDLDNDPVFLAQNGFSLNYEAERWKDIRFATIKDYQVRDKV
HHHHHCCCCCCEEEEECCCEECCCHHHHCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA