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The map label for this gene is ilvB [H]

Identifier: 225686705

GI number: 225686705

Start: 911643

End: 913337

Strand: Reverse

Name: ilvB [H]

Synonym: BMEA_B0930

Alternate gene names: 225686705

Gene position: 913337-911643 (Counterclockwise)

Preceding gene: 225686706

Following gene: 225686702

Centisome position: 77.04

GC content: 61.24

Gene sequence:

>1695_bases
ATGCATATCATGACGAGCAAACCAAATGTCCGTACCGGAGGCCAGATTTTGGTGGATGCGCTGCGCATCCATGGCGCGGA
CCGGGTCTTCTGCGTGCCCGGCGAAAGCTATCTCGCTGCGCTCGACGCCTTTCACGATGCAAGCGACGCCATCGACCTGA
TTGTCTGCCGGCAGGAGGGCGGGGCATCCTATATGGCAGAGGCCTATGGCAAACTGACGGGTAAGCCCGGCATTTGCTTC
GTCACGCGCGGCCCCGGCGCCACCAATGCATCGATTGGCGTGCACACGGCTTTTCAGGATTCGACGCCCATGCTGCTCTT
CATCGGTCAGGTCGCAAGCGATCAGGTCGAGCGTGAGGCATTTCAGGAAATCGATTATCGCCGCATGTTCGGCCAGATGG
CCAAATGGGTCGTGCAGATTGACGATGCCGCGCGCATTCCCGAACTGGTGAGCCAGGCATTCCATCGCGCGGTGAACGGG
CGTCCCGGTCCGGTGGTGGTCGCCTTGCCGGAAGACATGCTGACGTCCTATGCGGCGGTCAGCGATGCGCCTGCCTATAA
GCAGGTGGCCATGCATCCGGGTGGCGAACAGTTGCAGGCAATGCGGGCGCTGATCGAAAAGGCAGAGCGTCCGCTGATGA
TCGTCGGCGGCGGTGGCTGGACACAGGATGCCGTGCGCGACCTGCGCCAGTTTGCCGAAAATATGTCTCTGCCGGTTGCC
GCATCCTTCCGCTGTCAGGATATGTTCGACAATACGCATCCCCTTTATGCGGGCGAAATGGGGACCTCGATCAGTGCCAG
GCTTGTCCAGCGGGTGAAGGATTCCGACCTGCTTGTCGTGGTTGGCGCGCGCCTTGGTGAAATGACGACACAGGGCTATG
AACTCGTCACCATTCCGGTGCCGCAGCAGAAGCTTGTCCATATCCATCCGGGCGCGGAGGAACTGGGCCGGGTCTATCAT
GCCGATGTTCCGATCAATGCCGGGATGCCTGCCTTCTGTGCTGCGGCTGCAAAGCTTGCGCCGGTTGCCGATCCGCGCTG
GAAGGCATGGGCCGACAGCGCCAATGCCGACTATCGCGACAATATCAAGTCGCCGGTCATTCCGGGCGAAGTGCAGATGG
GCGAGGTGATGGAGTGGCTGCGCGCGCATCTGCCCGCCGATGCCATCATCACCAATGGCGCGGGCAATTATTCCGCATGG
CCGCATCGCTTTTACCAGTATCGCACCTATCGCAGCCAGTTGGCCCCGACCAATGGCTCCATGGGCTATGGTGTGCCGGC
TGCTGTTGCGGCCAAGCTCACCGCGCCGGAGCGGACCGTGGTGGCTTTCGCGGGAGATGGCTGTTTCCTCATGAACGGGC
AGGAGCTTGCAACTGCCGCGCAATATGGCGCGAATGCCATTTTCATCGTCGTGAACAACGGCATGTATGGCACGATCCGT
ATGCATCAGGAGCGCACCTATCCGGCGCGCATTTCGGGTACGGCCCTGGCCAATCCCGACTTTGCGGCGCTTGCGCGGGC
CTATGGGCTGCATGGTGAAGTAGTGGAAAAGACCGCTGATTTCGCACCCGCCTTCAAGCGGTGCGAACAGTCCGGCAAGC
CGGCGCTGATCGAGATCAGGATCGATCCCGAGGCACTGTCGCCCAAGATGAGCCTCAGCCAGATGCGGGAGCAGGGTTTC
GCGAAACAGCGCTGA

Upstream 100 bases:

>100_bases
TGGCTATGTTCATATCACCCATGCTTGACTGGGAGAAAAGCATTGTGCTGAAAACATATCTTCTGCTGCAAGGCGAGCCA
GATATGGTGAGATTGAGGAA

Downstream 100 bases:

>100_bases
CCGGGAGCCGCTTTAGATTACGTCGCGGATGCGCTCCACGCCAAGCTTGATGACGCTTTCCATGGCCAGGCGCGCCTTCT
GTGGATCGCGTGATGCAATG

Product: thiamine pyrophosphate protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MHIMTSKPNVRTGGQILVDALRIHGADRVFCVPGESYLAALDAFHDASDAIDLIVCRQEGGASYMAEAYGKLTGKPGICF
VTRGPGATNASIGVHTAFQDSTPMLLFIGQVASDQVEREAFQEIDYRRMFGQMAKWVVQIDDAARIPELVSQAFHRAVNG
RPGPVVVALPEDMLTSYAAVSDAPAYKQVAMHPGGEQLQAMRALIEKAERPLMIVGGGGWTQDAVRDLRQFAENMSLPVA
ASFRCQDMFDNTHPLYAGEMGTSISARLVQRVKDSDLLVVVGARLGEMTTQGYELVTIPVPQQKLVHIHPGAEELGRVYH
ADVPINAGMPAFCAAAAKLAPVADPRWKAWADSANADYRDNIKSPVIPGEVQMGEVMEWLRAHLPADAIITNGAGNYSAW
PHRFYQYRTYRSQLAPTNGSMGYGVPAAVAAKLTAPERTVVAFAGDGCFLMNGQELATAAQYGANAIFIVVNNGMYGTIR
MHQERTYPARISGTALANPDFAALARAYGLHGEVVEKTADFAPAFKRCEQSGKPALIEIRIDPEALSPKMSLSQMREQGF
AKQR

Sequences:

>Translated_564_residues
MHIMTSKPNVRTGGQILVDALRIHGADRVFCVPGESYLAALDAFHDASDAIDLIVCRQEGGASYMAEAYGKLTGKPGICF
VTRGPGATNASIGVHTAFQDSTPMLLFIGQVASDQVEREAFQEIDYRRMFGQMAKWVVQIDDAARIPELVSQAFHRAVNG
RPGPVVVALPEDMLTSYAAVSDAPAYKQVAMHPGGEQLQAMRALIEKAERPLMIVGGGGWTQDAVRDLRQFAENMSLPVA
ASFRCQDMFDNTHPLYAGEMGTSISARLVQRVKDSDLLVVVGARLGEMTTQGYELVTIPVPQQKLVHIHPGAEELGRVYH
ADVPINAGMPAFCAAAAKLAPVADPRWKAWADSANADYRDNIKSPVIPGEVQMGEVMEWLRAHLPADAIITNGAGNYSAW
PHRFYQYRTYRSQLAPTNGSMGYGVPAAVAAKLTAPERTVVAFAGDGCFLMNGQELATAAQYGANAIFIVVNNGMYGTIR
MHQERTYPARISGTALANPDFAALARAYGLHGEVVEKTADFAPAFKRCEQSGKPALIEIRIDPEALSPKMSLSQMREQGF
AKQR
>Mature_564_residues
MHIMTSKPNVRTGGQILVDALRIHGADRVFCVPGESYLAALDAFHDASDAIDLIVCRQEGGASYMAEAYGKLTGKPGICF
VTRGPGATNASIGVHTAFQDSTPMLLFIGQVASDQVEREAFQEIDYRRMFGQMAKWVVQIDDAARIPELVSQAFHRAVNG
RPGPVVVALPEDMLTSYAAVSDAPAYKQVAMHPGGEQLQAMRALIEKAERPLMIVGGGGWTQDAVRDLRQFAENMSLPVA
ASFRCQDMFDNTHPLYAGEMGTSISARLVQRVKDSDLLVVVGARLGEMTTQGYELVTIPVPQQKLVHIHPGAEELGRVYH
ADVPINAGMPAFCAAAAKLAPVADPRWKAWADSANADYRDNIKSPVIPGEVQMGEVMEWLRAHLPADAIITNGAGNYSAW
PHRFYQYRTYRSQLAPTNGSMGYGVPAAVAAKLTAPERTVVAFAGDGCFLMNGQELATAAQYGANAIFIVVNNGMYGTIR
MHQERTYPARISGTALANPDFAALARAYGLHGEVVEKTADFAPAFKRCEQSGKPALIEIRIDPEALSPKMSLSQMREQGF
AKQR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=24.6017699115044, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI21361361, Length=595, Percent_Identity=23.5294117647059, Blast_Score=110, Evalue=5e-24,
Organism=Escherichia coli, GI87081685, Length=515, Percent_Identity=30.0970873786408, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI1790104, Length=546, Percent_Identity=29.4871794871795, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1786717, Length=561, Percent_Identity=28.3422459893048, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787096, Length=556, Percent_Identity=25, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788716, Length=595, Percent_Identity=24.3697478991597, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17542570, Length=593, Percent_Identity=24.283305227656, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17531301, Length=568, Percent_Identity=24.1197183098592, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17531299, Length=568, Percent_Identity=24.2957746478873, Blast_Score=108, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323755, Length=562, Percent_Identity=28.6476868327402, Blast_Score=236, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6320816, Length=563, Percent_Identity=22.5577264653641, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19922626, Length=576, Percent_Identity=26.7361111111111, Blast_Score=151, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61128; Mature: 61128

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIMTSKPNVRTGGQILVDALRIHGADRVFCVPGESYLAALDAFHDASDAIDLIVCRQEG
CCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEEEECCC
GASYMAEAYGKLTGKPGICFVTRGPGATNASIGVHTAFQDSTPMLLFIGQVASDQVEREA
CCHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCEEEEEECHHHHHHHHHH
FQEIDYRRMFGQMAKWVVQIDDAARIPELVSQAFHRAVNGRPGPVVVALPEDMLTSYAAV
HHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHH
SDAPAYKQVAMHPGGEQLQAMRALIEKAERPLMIVGGGGWTQDAVRDLRQFAENMSLPVA
CCCCCHHHHEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEE
ASFRCQDMFDNTHPLYAGEMGTSISARLVQRVKDSDLLVVVGARLGEMTTQGYELVTIPV
ECCCHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCCEEEEECCHHCCCCCCCEEEEEECC
PQQKLVHIHPGAEELGRVYHADVPINAGMPAFCAAAAKLAPVADPRWKAWADSANADYRD
CCCCEEEECCCHHHHCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHH
NIKSPVIPGEVQMGEVMEWLRAHLPADAIITNGAGNYSAWPHRFYQYRTYRSQLAPTNGS
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC
MGYGVPAAVAAKLTAPERTVVAFAGDGCFLMNGQELATAAQYGANAIFIVVNNGMYGTIR
CCCCCHHHHHHCCCCCCEEEEEEECCCEEEECCHHHHHHHHCCCCEEEEEEECCEEEEEE
MHQERTYPARISGTALANPDFAALARAYGLHGEVVEKTADFAPAFKRCEQSGKPALIEIR
EECCCCCCCEECCCEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
IDPEALSPKMSLSQMREQGFAKQR
ECHHHCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHIMTSKPNVRTGGQILVDALRIHGADRVFCVPGESYLAALDAFHDASDAIDLIVCRQEG
CCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEEEECCC
GASYMAEAYGKLTGKPGICFVTRGPGATNASIGVHTAFQDSTPMLLFIGQVASDQVEREA
CCHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCEEEEEECHHHHHHHHHH
FQEIDYRRMFGQMAKWVVQIDDAARIPELVSQAFHRAVNGRPGPVVVALPEDMLTSYAAV
HHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHH
SDAPAYKQVAMHPGGEQLQAMRALIEKAERPLMIVGGGGWTQDAVRDLRQFAENMSLPVA
CCCCCHHHHEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEE
ASFRCQDMFDNTHPLYAGEMGTSISARLVQRVKDSDLLVVVGARLGEMTTQGYELVTIPV
ECCCHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCCEEEEECCHHCCCCCCCEEEEEECC
PQQKLVHIHPGAEELGRVYHADVPINAGMPAFCAAAAKLAPVADPRWKAWADSANADYRD
CCCCEEEECCCHHHHCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHH
NIKSPVIPGEVQMGEVMEWLRAHLPADAIITNGAGNYSAWPHRFYQYRTYRSQLAPTNGS
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC
MGYGVPAAVAAKLTAPERTVVAFAGDGCFLMNGQELATAAQYGANAIFIVVNNGMYGTIR
CCCCCHHHHHHCCCCCCEEEEEEECCCEEEECCHHHHHHHHCCCCEEEEEEECCEEEEEE
MHQERTYPARISGTALANPDFAALARAYGLHGEVVEKTADFAPAFKRCEQSGKPALIEIR
EECCCCCCCEECCCEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
IDPEALSPKMSLSQMREQGFAKQR
ECHHHCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]