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The map label for this gene is gcvA [H]

Identifier: 225686666

GI number: 225686666

Start: 866940

End: 867872

Strand: Reverse

Name: gcvA [H]

Synonym: BMEA_B0887

Alternate gene names: 225686666

Gene position: 867872-866940 (Counterclockwise)

Preceding gene: 225686668

Following gene: 225686665

Centisome position: 73.21

GC content: 56.7

Gene sequence:

>933_bases
ATGAAGCTGAGCAGGAGATTGATCCCGGACATTGCCACGCTGCAAGCCTTTGAATGTGCTGCCCGCCATGGCAGTTTCAC
GCAGGCCGCGCATGAGCTCAATCTCACCCAGAGTGCCGTCAGCCGACAGATCAAGGATCTGGAAAGCCAGTTGGGCGTGC
TCCTGTTCGAGCGCATTCGCCAGCGTATCCTGTTGTCCGATGCAGGGCGGAAATTCCTGCCGGAAGTGCGGCGCCTTCTT
AACCAGACAGAAGATACGATGCTGCGCGCCATGGCCTCGGCACAGTCCACCTCGTCATTCAGCATCGCAACCCTGCCGAC
ATTCGGCACACGCTGGCTGATGCCGCGCATTCTTGATTTCGTAGAAAAACATCCCGGCATTGCGCTCAATGTCGCTTCCC
GTTCCGGTGTCTTCGATTTCGAGGAGCAACCCTTCGATCTGGCGATCCATTATGGCCAGCCGGTCTGGGCCCACGCCACC
TGCACCTTCCTGTGCAGTGAGGTGATCGTTCCTGTTGCAAGCCCCAGTCTTTTGAAAACGCGCCGCCCCGCTTCGCCGGA
AGAGCTTGAGAACGAACCCCTGCTGCATCTTGCGACGCGACCCAAAATGTGGGCGCAATGGTTTGAAAGTTGTGGTGTGG
AGGGGCAGTCGGCCTATCGGGGCCATCGTTTTGAACAGTTCTCGATGGTGATCGGCGCGGCATTGGCTGGCCTGGGTTTT
GCGCTTTTGCCGCTTTATCTGATCGAACAGGAATTGCGGAGTGGCGAACTGGTGCTGCTTTTTGAAAAACCAATGCGAAC
CGAGAATGAATATTATCTCGTTGTGCCGGAGGGAAAACTGGAAAATCCGCTGACGCAGGTGTTCTGCCAGTGGATCGCCG
GGCAGGTCGGGAATACCGACTACTCGTCGTTGGTTCATTCCAAACCGGAATGA

Upstream 100 bases:

>100_bases
CTTGGGGGGGAACATGCATGGATTATGATGAAAATGTATCATATCAGCGGTTTTGCGGTTATGATCGCGACGAAAGCAAC
TATGTTAATGCGAGAATGGA

Downstream 100 bases:

>100_bases
ATTGTTGCGCAAATAACGCTGTCCAACCGGCTTTCGCGGCGTGAAATAGAAGCAGTCGGGCAGGGTGCCCGTGTTTCACT
ATTTTAATGGTTGATCTCAA

Product: hypothetical protein

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKLSRRLIPDIATLQAFECAARHGSFTQAAHELNLTQSAVSRQIKDLESQLGVLLFERIRQRILLSDAGRKFLPEVRRLL
NQTEDTMLRAMASAQSTSSFSIATLPTFGTRWLMPRILDFVEKHPGIALNVASRSGVFDFEEQPFDLAIHYGQPVWAHAT
CTFLCSEVIVPVASPSLLKTRRPASPEELENEPLLHLATRPKMWAQWFESCGVEGQSAYRGHRFEQFSMVIGAALAGLGF
ALLPLYLIEQELRSGELVLLFEKPMRTENEYYLVVPEGKLENPLTQVFCQWIAGQVGNTDYSSLVHSKPE

Sequences:

>Translated_310_residues
MKLSRRLIPDIATLQAFECAARHGSFTQAAHELNLTQSAVSRQIKDLESQLGVLLFERIRQRILLSDAGRKFLPEVRRLL
NQTEDTMLRAMASAQSTSSFSIATLPTFGTRWLMPRILDFVEKHPGIALNVASRSGVFDFEEQPFDLAIHYGQPVWAHAT
CTFLCSEVIVPVASPSLLKTRRPASPEELENEPLLHLATRPKMWAQWFESCGVEGQSAYRGHRFEQFSMVIGAALAGLGF
ALLPLYLIEQELRSGELVLLFEKPMRTENEYYLVVPEGKLENPLTQVFCQWIAGQVGNTDYSSLVHSKPE
>Mature_310_residues
MKLSRRLIPDIATLQAFECAARHGSFTQAAHELNLTQSAVSRQIKDLESQLGVLLFERIRQRILLSDAGRKFLPEVRRLL
NQTEDTMLRAMASAQSTSSFSIATLPTFGTRWLMPRILDFVEKHPGIALNVASRSGVFDFEEQPFDLAIHYGQPVWAHAT
CTFLCSEVIVPVASPSLLKTRRPASPEELENEPLLHLATRPKMWAQWFESCGVEGQSAYRGHRFEQFSMVIGAALAGLGF
ALLPLYLIEQELRSGELVLLFEKPMRTENEYYLVVPEGKLENPLTQVFCQWIAGQVGNTDYSSLVHSKPE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=34.006734006734, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=33.1010452961672, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1788706, Length=308, Percent_Identity=27.2727272727273, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI145693193, Length=297, Percent_Identity=27.6094276094276, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=25.7627118644068, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787589, Length=279, Percent_Identity=24.3727598566308, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693105, Length=124, Percent_Identity=34.6774193548387, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789639, Length=253, Percent_Identity=24.5059288537549, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI157672245, Length=117, Percent_Identity=38.4615384615385, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789204, Length=223, Percent_Identity=26.457399103139, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788887, Length=105, Percent_Identity=34.2857142857143, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790262, Length=300, Percent_Identity=27.3333333333333, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34890; Mature: 34890

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50931 HTH_LYSR ; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSRRLIPDIATLQAFECAARHGSFTQAAHELNLTQSAVSRQIKDLESQLGVLLFERIR
CCCCHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRILLSDAGRKFLPEVRRLLNQTEDTMLRAMASAQSTSSFSIATLPTFGTRWLMPRILDF
HHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
VEKHPGIALNVASRSGVFDFEEQPFDLAIHYGQPVWAHATCTFLCSEVIVPVASPSLLKT
HHHCCCEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHC
RRPASPEELENEPLLHLATRPKMWAQWFESCGVEGQSAYRGHRFEQFSMVIGAALAGLGF
CCCCCHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHH
ALLPLYLIEQELRSGELVLLFEKPMRTENEYYLVVPEGKLENPLTQVFCQWIAGQVGNTD
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCH
YSSLVHSKPE
HHHHHCCCCC
>Mature Secondary Structure
MKLSRRLIPDIATLQAFECAARHGSFTQAAHELNLTQSAVSRQIKDLESQLGVLLFERIR
CCCCHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRILLSDAGRKFLPEVRRLLNQTEDTMLRAMASAQSTSSFSIATLPTFGTRWLMPRILDF
HHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
VEKHPGIALNVASRSGVFDFEEQPFDLAIHYGQPVWAHATCTFLCSEVIVPVASPSLLKT
HHHCCCEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHC
RRPASPEELENEPLLHLATRPKMWAQWFESCGVEGQSAYRGHRFEQFSMVIGAALAGLGF
CCCCCHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHH
ALLPLYLIEQELRSGELVLLFEKPMRTENEYYLVVPEGKLENPLTQVFCQWIAGQVGNTD
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCH
YSSLVHSKPE
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]