Click here to switch to the map view.

The map label for this gene is leuD

Identifier: 225686645

GI number: 225686645

Start: 846067

End: 846672

Strand: Direct

Name: leuD

Synonym: BMEA_B0862

Alternate gene names: 225686645

Gene position: 846067-846672 (Clockwise)

Preceding gene: 225686644

Following gene: 225686646

Centisome position: 71.37

GC content: 56.27

Gene sequence:

>606_bases
ATGGATAAGTTCACCAAGCTCACGGGCGTCGCGGCCCCCCTGCCGATTGTCAATATCGACACGGACATGATCATTCCGAA
GGATTACCTGAAGACGATCAAGCGCACCGGGCTTGGCAAGGGCCTTTTCGCCGAAATGCGCTTCAATGAAGACGGGTCTG
AAAACCCGGATTTCGTGCTGAACAAGCCCGGCTACCGCAAGGCGCAGATTCTCGTCGCCGGCGACAATTTCGGTTGCGGC
TCTTCGCGCGAACATGCGCCATGGGCTCTGCTCGACTACGGCATCCGCTGCGTGATCTCGACCTCATTTGCCGATATTTT
CTACAATAATTGCTTCAAGAACGGCATCCTGCCGATCAAGGTCGCCCAAGAAGATCTGGACAAGCTGATGGACGACGCTT
CGCGCGGCGCAAATGCGACGCTGACCATCGATCTCGAAACCAAGCAGATTCATGGCCCCGATGGCGGCACGATCTCTTTC
GATCTCGACGATTTCAAGCGCCATTGCCTGTTGAACGGCCTCGACGATATCGGCCTGACCATGGAAAAGGCGAAGAGCAT
CGACACGTTCGAGGCGAAGAACGCTGAAGAACGCCCCTGGGCATGA

Upstream 100 bases:

>100_bases
GCGACCTATTGGGCAAGTTGTGGCCTGAAACGTTGACTCTTGGTCAAGCTGCTGTAAACCAATTTGCAAATTGCAAATAC
CTAACCGACAGGTTCAGAAC

Downstream 100 bases:

>100_bases
TGGTGGAAAGCATTATATATGGCCTGCAAGAGCCCAGTGGCCTCAAACGTGGCCTCAGACATTGAACAGGACACGATTGG
CTGAAATGGCCCGAGAAGAT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MDKFTKLTGVAAPLPIVNIDTDMIIPKDYLKTIKRTGLGKGLFAEMRFNEDGSENPDFVLNKPGYRKAQILVAGDNFGCG
SSREHAPWALLDYGIRCVISTSFADIFYNNCFKNGILPIKVAQEDLDKLMDDASRGANATLTIDLETKQIHGPDGGTISF
DLDDFKRHCLLNGLDDIGLTMEKAKSIDTFEAKNAEERPWA

Sequences:

>Translated_201_residues
MDKFTKLTGVAAPLPIVNIDTDMIIPKDYLKTIKRTGLGKGLFAEMRFNEDGSENPDFVLNKPGYRKAQILVAGDNFGCG
SSREHAPWALLDYGIRCVISTSFADIFYNNCFKNGILPIKVAQEDLDKLMDDASRGANATLTIDLETKQIHGPDGGTISF
DLDDFKRHCLLNGLDDIGLTMEKAKSIDTFEAKNAEERPWA
>Mature_201_residues
MDKFTKLTGVAAPLPIVNIDTDMIIPKDYLKTIKRTGLGKGLFAEMRFNEDGSENPDFVLNKPGYRKAQILVAGDNFGCG
SSREHAPWALLDYGIRCVISTSFADIFYNNCFKNGILPIKVAQEDLDKLMDDASRGANATLTIDLETKQIHGPDGGTISF
DLDDFKRHCLLNGLDDIGLTMEKAKSIDTFEAKNAEERPWA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=195, Percent_Identity=49.2307692307692, Blast_Score=198, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6321429, Length=204, Percent_Identity=52.4509803921569, Blast_Score=207, Evalue=6e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_BRUA1 (B2SAF6)

Other databases:

- EMBL:   CP000888
- RefSeq:   YP_001932289.1
- ProteinModelPortal:   B2SAF6
- SMR:   B2SAF6
- GeneID:   6326218
- GenomeReviews:   CP000888_GR
- KEGG:   bmc:BAbS19_II03340
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22237; Mature: 22237

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFTKLTGVAAPLPIVNIDTDMIIPKDYLKTIKRTGLGKGLFAEMRFNEDGSENPDFVL
CCCHHHHHCCCCCCCEEECCCCEECCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEE
NKPGYRKAQILVAGDNFGCGSSREHAPWALLDYGIRCVISTSFADIFYNNCFKNGILPIK
CCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHCCEEEEEEHHHHHHHHHHHHHCCCEEEE
VAQEDLDKLMDDASRGANATLTIDLETKQIHGPDGGTISFDLDDFKRHCLLNGLDDIGLT
ECHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCCC
MEKAKSIDTFEAKNAEERPWA
HHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDKFTKLTGVAAPLPIVNIDTDMIIPKDYLKTIKRTGLGKGLFAEMRFNEDGSENPDFVL
CCCHHHHHCCCCCCCEEECCCCEECCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEE
NKPGYRKAQILVAGDNFGCGSSREHAPWALLDYGIRCVISTSFADIFYNNCFKNGILPIK
CCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHCCEEEEEEHHHHHHHHHHHHHCCCEEEE
VAQEDLDKLMDDASRGANATLTIDLETKQIHGPDGGTISFDLDDFKRHCLLNGLDDIGLT
ECHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCCC
MEKAKSIDTFEAKNAEERPWA
HHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA