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The map label for this gene is puuA [H]

Identifier: 225686535

GI number: 225686535

Start: 727372

End: 728802

Strand: Direct

Name: puuA [H]

Synonym: BMEA_B0746

Alternate gene names: 225686535

Gene position: 727372-728802 (Clockwise)

Preceding gene: 225686534

Following gene: 225686536

Centisome position: 61.35

GC content: 58.21

Gene sequence:

>1431_bases
ATGTCTGCTGATACGACGGAAAAGAAGGCTACGCGCACACCGCGTCGCCGTACGCCTGCTTATGTCAAATCTCTGCGCGG
GGTGAAAAACTGGAAACAGGCAACTGAATGGCTGGCCTGGCGCGATATTGAAGATATTGAGTGCATTACCCCCGATCAGG
CGGGGGTTGCGCGCGGCAAGATGATGCCATCGAAGAAATTCACATCCAACACATCGCTGGCGCTGCCTTCCGCCGTTTTC
ATGACGACGATTTCCGGCGCTTACCCCGAGGATGGCTATGGTTTCCATTATCCCGAAGACGATGGCGACCTGAAGCTTCT
GCCCGATCTTACCACCCTCTCTGCCGTGCCATGGGAAACGGATTCGACGGCGCAGGTGATCTGCGATCTCGTCTATCAGG
ACGGGCGCGCGGTGGAATTCACCCCGCGCAATGTGCTGCGCAATGTCATTGCCGCCTATAGCAAGCGCGGCCTGAAGCCG
GTTGTCGCCCCGGAAATCGAGTTTTATCTGGTGCGCAAAAACCCGGATCCGGACTATCCGCTGACGCCGCCTGTTGGCCG
TTCGGGCCGGGCCATTGGTGGCGGACAGGGCTATTCCATTGCCGGTGTCAACGAGTTCGATGAACTGATCGACGATATCT
ATCATTTCTCGGAAGGTCAGGGGCTGGAGATCGACACGCTGATCCATGAAGAAGGTGCTGGCCAGCTTGAAATAAACCTG
CGCCATGGCGACCCGGTGGAACTGGCCGATCAGGTTTTCATGTTCAAGCGCACGATCCGCGAGGCGGCACTGAAACACGA
TATGTACGCCACGTTCATGGCCAAGCCCATTCAGGGCCAGCCGGGCTCGGCCATGCATATCCACCAGTCCATCGTGGACA
AGAAGACTGGACGAAACATCTTCACCAATGAAGACGGCAGCGAAAGCCAAGCCTTCCACCATTTCCTTGGCGGGATGCAG
CGCCATGTGCCCAATGCACTCGTCATGTTCGCCCCTTATGTGAACTCCTACCGCCGCCTCACGCCGGACGCCTCGGCCCC
GGTCAACGTCAAATGGGGCTATGACAACCGCACAACCGCCTTCCGCGTGCCGCGTTCAGACCCCAGTGCGCGCCGCGTGG
AAAACCGCATCCCCTCTTCCGACGCCAATCCCTACCTTGCCCTTGCTGCTTCGCTTGCTTGCGGCCTGATCGGCCTTGTA
AACAAGATCGAGCCGGAACAGCCCGCGACCACGAGCGTCAACACCAAGGAGGTCGAGTTGCCCCGCGGCCTCATCGACGC
GGTGGAGCTTTTCGAGGAAGACGCCGAATTGCGCAATCTGTTCGGCAGTTCCTTCATGACCACCTATGCCGCCATCAAAC
GCGCCGAGTTCGAGACCTTCATGGAAGTCATCAGCCCCTGGGAACGGGAGTTTTTGTTGCTCAATGTTTAA

Upstream 100 bases:

>100_bases
CAACAAAAATGATCCCTGCCCGCAGAAACGCAAAGGGCCATTTGTCAAGAGCGCCTGTCACAGCTATCATATCAATATCG
AAATGATTTTCTGGTGATGG

Downstream 100 bases:

>100_bases
GGGAGTAAGGGAGTAAGGGAGTAAGGGAGTAAAATACGCGGGGGCATAGTGATGTCAATCAATTCATATCGCGATATTCT
TGTGTAGCAGCAAGCAATGG

Product: glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSADTTEKKATRTPRRRTPAYVKSLRGVKNWKQATEWLAWRDIEDIECITPDQAGVARGKMMPSKKFTSNTSLALPSAVF
MTTISGAYPEDGYGFHYPEDDGDLKLLPDLTTLSAVPWETDSTAQVICDLVYQDGRAVEFTPRNVLRNVIAAYSKRGLKP
VVAPEIEFYLVRKNPDPDYPLTPPVGRSGRAIGGGQGYSIAGVNEFDELIDDIYHFSEGQGLEIDTLIHEEGAGQLEINL
RHGDPVELADQVFMFKRTIREAALKHDMYATFMAKPIQGQPGSAMHIHQSIVDKKTGRNIFTNEDGSESQAFHHFLGGMQ
RHVPNALVMFAPYVNSYRRLTPDASAPVNVKWGYDNRTTAFRVPRSDPSARRVENRIPSSDANPYLALAASLACGLIGLV
NKIEPEQPATTSVNTKEVELPRGLIDAVELFEEDAELRNLFGSSFMTTYAAIKRAEFETFMEVISPWEREFLLLNV

Sequences:

>Translated_476_residues
MSADTTEKKATRTPRRRTPAYVKSLRGVKNWKQATEWLAWRDIEDIECITPDQAGVARGKMMPSKKFTSNTSLALPSAVF
MTTISGAYPEDGYGFHYPEDDGDLKLLPDLTTLSAVPWETDSTAQVICDLVYQDGRAVEFTPRNVLRNVIAAYSKRGLKP
VVAPEIEFYLVRKNPDPDYPLTPPVGRSGRAIGGGQGYSIAGVNEFDELIDDIYHFSEGQGLEIDTLIHEEGAGQLEINL
RHGDPVELADQVFMFKRTIREAALKHDMYATFMAKPIQGQPGSAMHIHQSIVDKKTGRNIFTNEDGSESQAFHHFLGGMQ
RHVPNALVMFAPYVNSYRRLTPDASAPVNVKWGYDNRTTAFRVPRSDPSARRVENRIPSSDANPYLALAASLACGLIGLV
NKIEPEQPATTSVNTKEVELPRGLIDAVELFEEDAELRNLFGSSFMTTYAAIKRAEFETFMEVISPWEREFLLLNV
>Mature_475_residues
SADTTEKKATRTPRRRTPAYVKSLRGVKNWKQATEWLAWRDIEDIECITPDQAGVARGKMMPSKKFTSNTSLALPSAVFM
TTISGAYPEDGYGFHYPEDDGDLKLLPDLTTLSAVPWETDSTAQVICDLVYQDGRAVEFTPRNVLRNVIAAYSKRGLKPV
VAPEIEFYLVRKNPDPDYPLTPPVGRSGRAIGGGQGYSIAGVNEFDELIDDIYHFSEGQGLEIDTLIHEEGAGQLEINLR
HGDPVELADQVFMFKRTIREAALKHDMYATFMAKPIQGQPGSAMHIHQSIVDKKTGRNIFTNEDGSESQAFHHFLGGMQR
HVPNALVMFAPYVNSYRRLTPDASAPVNVKWGYDNRTTAFRVPRSDPSARRVENRIPSSDANPYLALAASLACGLIGLVN
KIEPEQPATTSVNTKEVELPRGLIDAVELFEEDAELRNLFGSSFMTTYAAIKRAEFETFMEVISPWEREFLLLNV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=437, Percent_Identity=25.858123569794, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI87081870, Length=475, Percent_Identity=32.6315789473684, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1790301, Length=413, Percent_Identity=28.5714285714286, Blast_Score=135, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 53096; Mature: 52964

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADTTEKKATRTPRRRTPAYVKSLRGVKNWKQATEWLAWRDIEDIECITPDQAGVARGK
CCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHCHHHCC
MMPSKKFTSNTSLALPSAVFMTTISGAYPEDGYGFHYPEDDGDLKLLPDLTTLSAVPWET
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCHHHHCCCCCCC
DSTAQVICDLVYQDGRAVEFTPRNVLRNVIAAYSKRGLKPVVAPEIEFYLVRKNPDPDYP
CCHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCCEECCCEEEEEEECCCCCCCC
LTPPVGRSGRAIGGGQGYSIAGVNEFDELIDDIYHFSEGQGLEIDTLIHEEGAGQLEINL
CCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEE
RHGDPVELADQVFMFKRTIREAALKHDMYATFMAKPIQGQPGSAMHIHQSIVDKKTGRNI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCC
FTNEDGSESQAFHHFLGGMQRHVPNALVMFAPYVNSYRRLTPDASAPVNVKWGYDNRTTA
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCCCCEEEEECCCCCCEE
FRVPRSDPSARRVENRIPSSDANPYLALAASLACGLIGLVNKIEPEQPATTSVNTKEVEL
EECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHC
PRGLIDAVELFEEDAELRNLFGSSFMTTYAAIKRAEFETFMEVISPWEREFLLLNV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure 
SADTTEKKATRTPRRRTPAYVKSLRGVKNWKQATEWLAWRDIEDIECITPDQAGVARGK
CCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHCHHHCC
MMPSKKFTSNTSLALPSAVFMTTISGAYPEDGYGFHYPEDDGDLKLLPDLTTLSAVPWET
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCHHHHCCCCCCC
DSTAQVICDLVYQDGRAVEFTPRNVLRNVIAAYSKRGLKPVVAPEIEFYLVRKNPDPDYP
CCHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCCEECCCEEEEEEECCCCCCCC
LTPPVGRSGRAIGGGQGYSIAGVNEFDELIDDIYHFSEGQGLEIDTLIHEEGAGQLEINL
CCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEE
RHGDPVELADQVFMFKRTIREAALKHDMYATFMAKPIQGQPGSAMHIHQSIVDKKTGRNI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCC
FTNEDGSESQAFHHFLGGMQRHVPNALVMFAPYVNSYRRLTPDASAPVNVKWGYDNRTTA
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCCCCEEEEECCCCCCEE
FRVPRSDPSARRVENRIPSSDANPYLALAASLACGLIGLVNKIEPEQPATTSVNTKEVEL
EECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHC
PRGLIDAVELFEEDAELRNLFGSSFMTTYAAIKRAEFETFMEVISPWEREFLLLNV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]