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The map label for this gene is rlmE

Identifier: 225686460

GI number: 225686460

Start: 645213

End: 645935

Strand: Direct

Name: rlmE

Synonym: BMEA_B0657

Alternate gene names: 225686460

Gene position: 645213-645935 (Clockwise)

Preceding gene: 225686459

Following gene: 225686468

Centisome position: 54.42

GC content: 60.44

Gene sequence:

>723_bases
ATGAGCAAAGCAGGCGGAAACAAGGGTGGCAGCAGGACGGGCGGCCGCGGCGGCGCGGGCAGCAGCAACCTGCATGTGCG
GGTGAAGAAAAAGGCCGGCACGATCAAGGAATCCTCCCGCCGCTGGCTGGAGCGTCACCTGAACGACCCCTATGTGCACA
AATCCCGGCAGGACGGCTATCGTTCGCGCGCAGCCTACAAGCTCATCGAGATCAATGATCGCTACAACCTCCTGAAAAAG
GGGCAGAAGATCATCGACCTCGGCGCCGCCCCCGGCGGCTGGTCACAGATCGCGGCCAGGATTGTCGGCTCGACCGATGA
GAATCCACAGGTCGTCGGCATTGATTATCTGCATGTCGATCCCCTGCCCGGCGTCATTCTGCTGGAAATGGATTTTCTAG
ATGATGAAGCGCCGCAAAAGCTGATGGACGCCCTTGGCGACAAGCCGGACCTCGTCATTTCAGACATGGCCGCGCCCACG
ACAGGCCATCGCCGGACGGACCATCTGCGCACGGTGCACCTCTGCGAGGTTGCCGCCGATTTTGCGGTTTCGGTGCTGAA
ACCCGGTGGCCACTTCCTGACCAAGACATTTCAGGGCGGCACGGAAAACGAGCTTCTGGCGCTGCTCAAGCAGAAGTTCC
GCTCGGTCCATCATGTGAAGCCGCCAGCATCTCGCGCAGAATCGGTGGAACTTTACCTGCTCGCCCGCGACTTCAAAGGT
TAA

Upstream 100 bases:

>100_bases
GCCGATCGCGGTTTGCGCGAAGGCATTCTGACCGAACTGATGTCACGCGACGGCGCATGGCGCCATAACCGGGCATCCGG
CGCTAGAAACAGGCACTAAG

Downstream 100 bases:

>100_bases
AGCAGTTTCAGCGTTTCCGTCTTAACCGGAACCGCTCTGGTGGTCTGATTCCAACATTTGCATCCCTCGGTTCAAGCGTT
GAGCAAATGTTGGAATCACA

Product: Ribosomal RNA large subunit methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MSKAGGNKGGSRTGGRGGAGSSNLHVRVKKKAGTIKESSRRWLERHLNDPYVHKSRQDGYRSRAAYKLIEINDRYNLLKK
GQKIIDLGAAPGGWSQIAARIVGSTDENPQVVGIDYLHVDPLPGVILLEMDFLDDEAPQKLMDALGDKPDLVISDMAAPT
TGHRRTDHLRTVHLCEVAADFAVSVLKPGGHFLTKTFQGGTENELLALLKQKFRSVHHVKPPASRAESVELYLLARDFKG

Sequences:

>Translated_240_residues
MSKAGGNKGGSRTGGRGGAGSSNLHVRVKKKAGTIKESSRRWLERHLNDPYVHKSRQDGYRSRAAYKLIEINDRYNLLKK
GQKIIDLGAAPGGWSQIAARIVGSTDENPQVVGIDYLHVDPLPGVILLEMDFLDDEAPQKLMDALGDKPDLVISDMAAPT
TGHRRTDHLRTVHLCEVAADFAVSVLKPGGHFLTKTFQGGTENELLALLKQKFRSVHHVKPPASRAESVELYLLARDFKG
>Mature_239_residues
SKAGGNKGGSRTGGRGGAGSSNLHVRVKKKAGTIKESSRRWLERHLNDPYVHKSRQDGYRSRAAYKLIEINDRYNLLKKG
QKIIDLGAAPGGWSQIAARIVGSTDENPQVVGIDYLHVDPLPGVILLEMDFLDDEAPQKLMDALGDKPDLVISDMAAPTT
GHRRTDHLRTVHLCEVAADFAVSVLKPGGHFLTKTFQGGTENELLALLKQKFRSVHHVKPPASRAESVELYLLARDFKG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=206, Percent_Identity=40.2912621359223, Blast_Score=170, Evalue=8e-43,
Organism=Homo sapiens, GI194097365, Length=192, Percent_Identity=37.5, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI29029591, Length=191, Percent_Identity=31.9371727748691, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI29029589, Length=191, Percent_Identity=31.9371727748691, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI7110661, Length=191, Percent_Identity=31.9371727748691, Blast_Score=108, Evalue=3e-24,
Organism=Escherichia coli, GI1789569, Length=199, Percent_Identity=40.7035175879397, Blast_Score=149, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17553474, Length=205, Percent_Identity=37.0731707317073, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17554650, Length=195, Percent_Identity=35.3846153846154, Blast_Score=128, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17553860, Length=193, Percent_Identity=32.6424870466321, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71987561, Length=189, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71987550, Length=193, Percent_Identity=32.6424870466321, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6319796, Length=195, Percent_Identity=33.8461538461538, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319535, Length=195, Percent_Identity=31.2820512820513, Blast_Score=105, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321302, Length=113, Percent_Identity=38.9380530973451, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356387, Length=211, Percent_Identity=39.8104265402844, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI18859957, Length=192, Percent_Identity=35.4166666666667, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=29.7979797979798, Blast_Score=110, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24648639, Length=196, Percent_Identity=29.0816326530612, Blast_Score=103, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_BRUA1 (B2SAY0)

Other databases:

- EMBL:   CP000888
- RefSeq:   YP_001932463.1
- ProteinModelPortal:   B2SAY0
- SMR:   B2SAY0
- GeneID:   6326230
- GenomeReviews:   CP000888_GR
- KEGG:   bmc:BAbS19_II05220
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- ProtClustDB:   CLSK898885
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 26308; Mature: 26177

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: ACT_SITE 195-195 BINDING 92-92 BINDING 94-94 BINDING 115-115 BINDING 131-131 BINDING 155-155

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAGGNKGGSRTGGRGGAGSSNLHVRVKKKAGTIKESSRRWLERHLNDPYVHKSRQDGY
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
RSRAAYKLIEINDRYNLLKKGQKIIDLGAAPGGWSQIAARIVGSTDENPQVVGIDYLHVD
HHHHEEEEEEECCHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEEEEEEC
PLPGVILLEMDFLDDEAPQKLMDALGDKPDLVISDMAAPTTGHRRTDHLRTVHLCEVAAD
CCCCEEEEEEECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
FAVSVLKPGGHFLTKTFQGGTENELLALLKQKFRSVHHVKPPASRAESVELYLLARDFKG
HHHHHHCCCCCEEEHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCC
>Mature Secondary Structure 
SKAGGNKGGSRTGGRGGAGSSNLHVRVKKKAGTIKESSRRWLERHLNDPYVHKSRQDGY
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
RSRAAYKLIEINDRYNLLKKGQKIIDLGAAPGGWSQIAARIVGSTDENPQVVGIDYLHVD
HHHHEEEEEEECCHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEEEEEEC
PLPGVILLEMDFLDDEAPQKLMDALGDKPDLVISDMAAPTTGHRRTDHLRTVHLCEVAAD
CCCCEEEEEEECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
FAVSVLKPGGHFLTKTFQGGTENELLALLKQKFRSVHHVKPPASRAESVELYLLARDFKG
HHHHHHCCCCCEEEHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA