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The map label for this gene is dinB

Identifier: 225686400

GI number: 225686400

Start: 580557

End: 581894

Strand: Direct

Name: dinB

Synonym: BMEA_B0590

Alternate gene names: 225686400

Gene position: 580557-581894 (Clockwise)

Preceding gene: 225686399

Following gene: 225686401

Centisome position: 48.97

GC content: 59.19

Gene sequence:

>1338_bases
ATGGCCGAAAGCCCTATCGTAAACCATCCCGAACAGGGCCTGTGCCGCGACTGCCTGTCGCTGCAAAAGACGCAGACATC
GCGGCGGTGCCATGCTTGCGGCAGTCCCCGGCTGATCCGCCACAAGGAGCTTTACCGGCTCTCGCTCGCCCATGTGGATT
GCGACGCCTTCTATGCCTCGGTGGAAAAGCGTGACAATCCCGATCTGCGCGACAAGCCGCTGATTGTTGGCGGTGGAAAA
CGCGGCGTCGTCTCCACCGCCTGTTATCTGGCGCGCATTCATGGCGTGCGCTCCGCCATGCCCATGTTCAAGGCGCTGGA
AGCCTGCCCCGATGCCGTGGTTATCAAGCCGAACATGGAGAAATATGCACGGGTGGGCCGTGAAGTGCGCCAGATGATGC
GCGATCTGACCCCGCTCGTGGAGCCGATTTCCATCGATGAAGCCTTTCTCGATCTGAGCGGCACGGAACGGCTGCACAAG
GCTCCACCGGCCGTGGTTCTGGCGCGCTTTTCCAAACGTGTCGAGAATGAAATCGGCATTACGGCTTCGATCGGGCTTTC
CTATTGCAAATATCTGGCAAAGGTTGCGTCCGACCTTGAAAAACCGCGTGGTTTTTCCGTCATCGGTGAAGCGGAGGCGC
TCGATTTTCTGCGCGACAAGCCAGTCGGCATGATCTGGGGCGTCGGCAAGGCCTTTGCCGCCAAGCTGGAGAGCGACGGC
ATCCGCACCATCGGACAATTGCAGACAATGGAAGAAGGCGCGCTGATGAAGGCATATGGCACCATGGGCCAGCGGCTCTA
TCGCCTGTCACGCGGGCAGGACAGCCGCAAGGTGGAGCCGGACCACGACATGAAAAGCGTTTCGGCGGAAACCACCTTCA
ACACCGATCTTTCGGCGGCAGGTGATCTGGTTCCCGTATTGCGCGCCCTCTCCGAAAAAGTTTCCCGCCGCCTGAAAGCA
GGTGAAATTGCCGGGCGAACCATTGTGCTGAAGCTCAAGACGCAGGATTTCAAACTGCGCACACGCAACCGCCAGCTTGG
CGACCCAACGCAGCTTGCCGACCGGATTTTCCGCACCGGCCTCCAACTTCTCGAAAAGGAAATGGACGGAACCCGCTTCC
GCTTGCTTGGCATTGGCGTCAGCGATCTTTCGCCAAGCGACCGGGCCGATCCACCTGACCTCGTTGATATTCAGGCCACC
AAGCGCGCTGTCGCGGAAAGCGCCATCGACCGGCTGCGCAACAAATTCGGCCTCAATGCAGTTGAAACCGGCTACACTTT
TTCCAAGGGCAATCTGGCGCGAACACAAACACCAACCGACCGCGATAATGAACCTTGA

Upstream 100 bases:

>100_bases
AAGGGCAGCACTGACAGGGCCGCTATAGCCGATGGTTTATGCCGCAAATGATGCTATAAGGAGATGTTCCTTTTATGTTC
ATTGAACCGTCGCGTTGATC

Downstream 100 bases:

>100_bases
TAAATTTTTAAAGTAATTCATACAATATATTGATTAATATTACTTTTATTTCATGATAATTTCATCTCACATGGCTACTG
TCATCGTTTCTTCGTGAAAC

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MAESPIVNHPEQGLCRDCLSLQKTQTSRRCHACGSPRLIRHKELYRLSLAHVDCDAFYASVEKRDNPDLRDKPLIVGGGK
RGVVSTACYLARIHGVRSAMPMFKALEACPDAVVIKPNMEKYARVGREVRQMMRDLTPLVEPISIDEAFLDLSGTERLHK
APPAVVLARFSKRVENEIGITASIGLSYCKYLAKVASDLEKPRGFSVIGEAEALDFLRDKPVGMIWGVGKAFAAKLESDG
IRTIGQLQTMEEGALMKAYGTMGQRLYRLSRGQDSRKVEPDHDMKSVSAETTFNTDLSAAGDLVPVLRALSEKVSRRLKA
GEIAGRTIVLKLKTQDFKLRTRNRQLGDPTQLADRIFRTGLQLLEKEMDGTRFRLLGIGVSDLSPSDRADPPDLVDIQAT
KRAVAESAIDRLRNKFGLNAVETGYTFSKGNLARTQTPTDRDNEP

Sequences:

>Translated_445_residues
MAESPIVNHPEQGLCRDCLSLQKTQTSRRCHACGSPRLIRHKELYRLSLAHVDCDAFYASVEKRDNPDLRDKPLIVGGGK
RGVVSTACYLARIHGVRSAMPMFKALEACPDAVVIKPNMEKYARVGREVRQMMRDLTPLVEPISIDEAFLDLSGTERLHK
APPAVVLARFSKRVENEIGITASIGLSYCKYLAKVASDLEKPRGFSVIGEAEALDFLRDKPVGMIWGVGKAFAAKLESDG
IRTIGQLQTMEEGALMKAYGTMGQRLYRLSRGQDSRKVEPDHDMKSVSAETTFNTDLSAAGDLVPVLRALSEKVSRRLKA
GEIAGRTIVLKLKTQDFKLRTRNRQLGDPTQLADRIFRTGLQLLEKEMDGTRFRLLGIGVSDLSPSDRADPPDLVDIQAT
KRAVAESAIDRLRNKFGLNAVETGYTFSKGNLARTQTPTDRDNEP
>Mature_444_residues
AESPIVNHPEQGLCRDCLSLQKTQTSRRCHACGSPRLIRHKELYRLSLAHVDCDAFYASVEKRDNPDLRDKPLIVGGGKR
GVVSTACYLARIHGVRSAMPMFKALEACPDAVVIKPNMEKYARVGREVRQMMRDLTPLVEPISIDEAFLDLSGTERLHKA
PPAVVLARFSKRVENEIGITASIGLSYCKYLAKVASDLEKPRGFSVIGEAEALDFLRDKPVGMIWGVGKAFAAKLESDGI
RTIGQLQTMEEGALMKAYGTMGQRLYRLSRGQDSRKVEPDHDMKSVSAETTFNTDLSAAGDLVPVLRALSEKVSRRLKAG
EIAGRTIVLKLKTQDFKLRTRNRQLGDPTQLADRIFRTGLQLLEKEMDGTRFRLLGIGVSDLSPSDRADPPDLVDIQATK
RAVAESAIDRLRNKFGLNAVETGYTFSKGNLARTQTPTDRDNEP

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain

Homologues:

Organism=Homo sapiens, GI84043967, Length=342, Percent_Identity=27.1929824561404, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI7706681, Length=343, Percent_Identity=27.1137026239067, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI7705344, Length=106, Percent_Identity=45.2830188679245, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI154350220, Length=307, Percent_Identity=27.3615635179153, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1786425, Length=342, Percent_Identity=37.4269005847953, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1787432, Length=356, Percent_Identity=25.2808988764045, Blast_Score=95, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17537959, Length=297, Percent_Identity=24.9158249158249, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI193205702, Length=226, Percent_Identity=33.1858407079646, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI193205700, Length=226, Percent_Identity=33.1858407079646, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21355641, Length=285, Percent_Identity=27.3684210526316, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24644984, Length=285, Percent_Identity=27.3684210526316, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19923006, Length=343, Percent_Identity=24.7813411078717, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPO4_BRUME (Q8YC76)

Other databases:

- EMBL:   AE008918
- PIR:   AG3591
- RefSeq:   NP_541634.1
- ProteinModelPortal:   Q8YC76
- SMR:   Q8YC76
- GeneID:   1198428
- GenomeReviews:   AE008918_GR
- KEGG:   bme:BMEII0656
- HOGENOM:   HBG734504
- OMA:   ACYIARI
- PhylomeDB:   Q8YC76
- ProtClustDB:   PRK02794
- BioCyc:   BMEL224914:BMEII0656-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005737
- HAMAP:   MF_01113
- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880
- Gene3D:   G3DSA:3.30.1490.100
- PANTHER:   PTHR11076

Pfam domain/function: PF00817 IMS; SSF100879 DNA_pol_Y-fam_little_finger

EC number: =2.7.7.7

Molecular weight: Translated: 49267; Mature: 49136

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50173 UMUC

Important sites: ACT_SITE 147-147

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESPIVNHPEQGLCRDCLSLQKTQTSRRCHACGSPRLIRHKELYRLSLAHVDCDAFYAS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
VEKRDNPDLRDKPLIVGGGKRGVVSTACYLARIHGVRSAMPMFKALEACPDAVVIKPNME
HHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHH
KYARVGREVRQMMRDLTPLVEPISIDEAFLDLSGTERLHKAPPAVVLARFSKRVENEIGI
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHCCC
TASIGLSYCKYLAKVASDLEKPRGFSVIGEAEALDFLRDKPVGMIWGVGKAFAAKLESDG
CHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCEEEECCHHHHHHHHHCCC
IRTIGQLQTMEEGALMKAYGTMGQRLYRLSRGQDSRKVEPDHDMKSVSAETTFNTDLSAA
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCHHCC
GDLVPVLRALSEKVSRRLKAGEIAGRTIVLKLKTQDFKLRTRNRQLGDPTQLADRIFRTG
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHH
LQLLEKEMDGTRFRLLGIGVSDLSPSDRADPPDLVDIQATKRAVAESAIDRLRNKFGLNA
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCE
VETGYTFSKGNLARTQTPTDRDNEP
ECCCCEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AESPIVNHPEQGLCRDCLSLQKTQTSRRCHACGSPRLIRHKELYRLSLAHVDCDAFYAS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
VEKRDNPDLRDKPLIVGGGKRGVVSTACYLARIHGVRSAMPMFKALEACPDAVVIKPNME
HHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHH
KYARVGREVRQMMRDLTPLVEPISIDEAFLDLSGTERLHKAPPAVVLARFSKRVENEIGI
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHCCC
TASIGLSYCKYLAKVASDLEKPRGFSVIGEAEALDFLRDKPVGMIWGVGKAFAAKLESDG
CHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCEEEECCHHHHHHHHHCCC
IRTIGQLQTMEEGALMKAYGTMGQRLYRLSRGQDSRKVEPDHDMKSVSAETTFNTDLSAA
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCHHCC
GDLVPVLRALSEKVSRRLKAGEIAGRTIVLKLKTQDFKLRTRNRQLGDPTQLADRIFRTG
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHH
LQLLEKEMDGTRFRLLGIGVSDLSPSDRADPPDLVDIQATKRAVAESAIDRLRNKFGLNA
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCE
VETGYTFSKGNLARTQTPTDRDNEP
ECCCCEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688