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The map label for this gene is pleD [H]

Identifier: 225686396

GI number: 225686396

Start: 577197

End: 578582

Strand: Reverse

Name: pleD [H]

Synonym: BMEA_B0585

Alternate gene names: 225686396

Gene position: 578582-577197 (Counterclockwise)

Preceding gene: 225686397

Following gene: 225686389

Centisome position: 48.8

GC content: 56.64

Gene sequence:

>1386_bases
ATGACAGCAAGGATTCTCGTCGTCGATGATGTGGACTCCAATGTAAAGCTTCTCGAAGCGCGCCTTCTCAGCGAATATTA
CGAAGTCGTTCCCGCCTATAGCGGCGCCGAGGCGATTGAAATCTGTCTTGATGGGCAGATCGACATTGTTCTTCTGGATG
TATTGATGCCGGGGCTTGATGGTTTCGAGGTTTGCCGACGCCTGAAAGCCAATCCGCGTACGGCAAATATTCCAGTCATT
ATGATTACGTGCCTCACCAGCCCGGAAGACAAGGTGCGCGGGCTTGAGGCGGGGGCGGAAGATTTTCTCTCAAAGCCTGT
CAACGATCTTGAACTCCTGTCACGGCTGAAAAGCCTTACCCGGCTCAAGATGATGTCGGACGAGCTGTTCCAGCGCGCCG
GCCGGATTGCGGATGCCGAAGTGGATGCCCTCCTTGCGCGCAAGATGGCGGGTAAAGACGGCAATAAAGATGAGATCGCC
CGCATTCTTGTGGTGGATGAGGATGAACTGGCGGCCGCGCGCCTTCGCCATATTCTGGATGAGAATTATCGGGTCGATGT
GGCGAGTGACGCCGAAACCGCACTGATCCGCGCTATTGAAACAGATTACGACACGATCATCGTTTCGGCCTCCTTCACCT
ATTATGATCCGCTGAAACTATGCACGCAATTGCGCACAATCCAGCGCACGCGGCTCATACCGATCATCCTGATGGTGTGG
GAAGACGAGGGCGCACTGGTGGTGCGCGCACTGGAACTGGGCGTGAACGACTATCTGATGCGCCCATTGGAGAAGATAGA
ACTGTTTGCGCGGCTGCGCACACAGATCAAGCGCAAATGCTATAATGATATTCTGCGCCAGAGCATGGAGCGCACTATTA
CCCAATCGGTGACGGACGGCCTCACGGGGCTGCATAACCGCCGCTACATCGACATGCACATGCCGCTGCTGCTCACACGC
GCAATCGAGCGCAAGCAGCCCCTCTCGATCATCATGATCGATCTTGATCACTTCAAGCAGGTCAACGAACAATATGGCCA
TGGCGCGGGCGATCATGTGTTGCGGGAATTTTCGGGCCGTTTGCGCAGGAATATTCGCGGCATGGATCTTATCAGCCGCT
ACGGTGGTGAGGAATTTGTCGTCGTGCTGCCGGACACGGACAGGCAGGCTGCCTTCAATGTGGCTGAGCGCGTGCGGGGT
ATCGTAGCCGAAGCTCCCTTTGTGCTCGATGACGGAAAGCGCAGGGCCAGCCTGACGGTCAGCGTGGGCGTTGCAGCACT
TCGGCCCGCAGGCGACAGCCTTGAGGCGCTTTTCACCCGCGCGACCGACGCGCTCATTCAGGCGAAGCAGAGCGGACGAA
ATCGCATTGTTTTATCCGCGGCCTGA

Upstream 100 bases:

>100_bases
AATCGCCGGAACGCTCTAACTATTTGTTTTGTCGCATTATCCGACGCAAAACCGCTTCGCATTTTTGCTGGAAATGCTCT
AAAACTTTCAAGGATGCGCA

Downstream 100 bases:

>100_bases
TTGCGCAAATGCGAAGGCGCGATTCCAGGTTGGAAAAATAAAAAGCCGCCTCGCAAGAGACGGCTTTTTGAATTCAATCG
TCTGTCGATGACGACCGGAA

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTARILVVDDVDSNVKLLEARLLSEYYEVVPAYSGAEAIEICLDGQIDIVLLDVLMPGLDGFEVCRRLKANPRTANIPVI
MITCLTSPEDKVRGLEAGAEDFLSKPVNDLELLSRLKSLTRLKMMSDELFQRAGRIADAEVDALLARKMAGKDGNKDEIA
RILVVDEDELAAARLRHILDENYRVDVASDAETALIRAIETDYDTIIVSASFTYYDPLKLCTQLRTIQRTRLIPIILMVW
EDEGALVVRALELGVNDYLMRPLEKIELFARLRTQIKRKCYNDILRQSMERTITQSVTDGLTGLHNRRYIDMHMPLLLTR
AIERKQPLSIIMIDLDHFKQVNEQYGHGAGDHVLREFSGRLRRNIRGMDLISRYGGEEFVVVLPDTDRQAAFNVAERVRG
IVAEAPFVLDDGKRRASLTVSVGVAALRPAGDSLEALFTRATDALIQAKQSGRNRIVLSAA

Sequences:

>Translated_461_residues
MTARILVVDDVDSNVKLLEARLLSEYYEVVPAYSGAEAIEICLDGQIDIVLLDVLMPGLDGFEVCRRLKANPRTANIPVI
MITCLTSPEDKVRGLEAGAEDFLSKPVNDLELLSRLKSLTRLKMMSDELFQRAGRIADAEVDALLARKMAGKDGNKDEIA
RILVVDEDELAAARLRHILDENYRVDVASDAETALIRAIETDYDTIIVSASFTYYDPLKLCTQLRTIQRTRLIPIILMVW
EDEGALVVRALELGVNDYLMRPLEKIELFARLRTQIKRKCYNDILRQSMERTITQSVTDGLTGLHNRRYIDMHMPLLLTR
AIERKQPLSIIMIDLDHFKQVNEQYGHGAGDHVLREFSGRLRRNIRGMDLISRYGGEEFVVVLPDTDRQAAFNVAERVRG
IVAEAPFVLDDGKRRASLTVSVGVAALRPAGDSLEALFTRATDALIQAKQSGRNRIVLSAA
>Mature_460_residues
TARILVVDDVDSNVKLLEARLLSEYYEVVPAYSGAEAIEICLDGQIDIVLLDVLMPGLDGFEVCRRLKANPRTANIPVIM
ITCLTSPEDKVRGLEAGAEDFLSKPVNDLELLSRLKSLTRLKMMSDELFQRAGRIADAEVDALLARKMAGKDGNKDEIAR
ILVVDEDELAAARLRHILDENYRVDVASDAETALIRAIETDYDTIIVSASFTYYDPLKLCTQLRTIQRTRLIPIILMVWE
DEGALVVRALELGVNDYLMRPLEKIELFARLRTQIKRKCYNDILRQSMERTITQSVTDGLTGLHNRRYIDMHMPLLLTRA
IERKQPLSIIMIDLDHFKQVNEQYGHGAGDHVLREFSGRLRRNIRGMDLISRYGGEEFVVVLPDTDRQAAFNVAERVRGI
VAEAPFVLDDGKRRASLTVSVGVAALRPAGDSLEALFTRATDALIQAKQSGRNRIVLSAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=183, Percent_Identity=36.0655737704918, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI87082007, Length=177, Percent_Identity=35.5932203389831, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081881, Length=160, Percent_Identity=40, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787262, Length=196, Percent_Identity=31.1224489795918, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1786584, Length=194, Percent_Identity=30.9278350515464, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787802, Length=143, Percent_Identity=32.8671328671329, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=30.4093567251462, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=33.1325301204819, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786599, Length=155, Percent_Identity=32.9032258064516, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081977, Length=140, Percent_Identity=35, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789809, Length=119, Percent_Identity=36.9747899159664, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788085, Length=180, Percent_Identity=28.3333333333333, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1786784, Length=151, Percent_Identity=31.7880794701987, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788394, Length=131, Percent_Identity=32.0610687022901, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI2367329, Length=160, Percent_Identity=38.125, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787541, Length=175, Percent_Identity=30.8571428571429, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI87081974, Length=172, Percent_Identity=26.7441860465116, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082012, Length=119, Percent_Identity=33.6134453781513, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790863, Length=165, Percent_Identity=30.3030303030303, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=41.747572815534, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790437, Length=105, Percent_Identity=40.9523809523809, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788550, Length=131, Percent_Identity=31.2977099236641, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1789149, Length=105, Percent_Identity=32.3809523809524, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51747; Mature: 51615

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARILVVDDVDSNVKLLEARLLSEYYEVVPAYSGAEAIEICLDGQIDIVLLDVLMPGLD
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEHHHHHCCCC
GFEVCRRLKANPRTANIPVIMITCLTSPEDKVRGLEAGAEDFLSKPVNDLELLSRLKSLT
HHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHH
RLKMMSDELFQRAGRIADAEVDALLARKMAGKDGNKDEIARILVVDEDELAAARLRHILD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHEEEEEEECCHHHHHHHHHHHHC
ENYRVDVASDAETALIRAIETDYDTIIVSASFTYYDPLKLCTQLRTIQRTRLIPIILMVW
CCCEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCEEEEEEEEE
EDEGALVVRALELGVNDYLMRPLEKIELFARLRTQIKRKCYNDILRQSMERTITQSVTDG
ECCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTGLHNRRYIDMHMPLLLTRAIERKQPLSIIMIDLDHFKQVNEQYGHGAGDHVLREFSGR
HHHCCCCEEEEEHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHH
LRRNIRGMDLISRYGGEEFVVVLPDTDRQAAFNVAERVRGIVAEAPFVLDDGKRRASLTV
HHHCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEE
SVGVAALRPAGDSLEALFTRATDALIQAKQSGRNRIVLSAA
EECCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
>Mature Secondary Structure 
TARILVVDDVDSNVKLLEARLLSEYYEVVPAYSGAEAIEICLDGQIDIVLLDVLMPGLD
CEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEHHHHHCCCC
GFEVCRRLKANPRTANIPVIMITCLTSPEDKVRGLEAGAEDFLSKPVNDLELLSRLKSLT
HHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHH
RLKMMSDELFQRAGRIADAEVDALLARKMAGKDGNKDEIARILVVDEDELAAARLRHILD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHEEEEEEECCHHHHHHHHHHHHC
ENYRVDVASDAETALIRAIETDYDTIIVSASFTYYDPLKLCTQLRTIQRTRLIPIILMVW
CCCEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCEEEEEEEEE
EDEGALVVRALELGVNDYLMRPLEKIELFARLRTQIKRKCYNDILRQSMERTITQSVTDG
ECCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTGLHNRRYIDMHMPLLLTRAIERKQPLSIIMIDLDHFKQVNEQYGHGAGDHVLREFSGR
HHHCCCCEEEEEHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHH
LRRNIRGMDLISRYGGEEFVVVLPDTDRQAAFNVAERVRGIVAEAPFVLDDGKRRASLTV
HHHCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEE
SVGVAALRPAGDSLEALFTRATDALIQAKQSGRNRIVLSAA
EECCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA