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The map label for this gene is gatA [H]

Identifier: 225686380

GI number: 225686380

Start: 559796

End: 561277

Strand: Reverse

Name: gatA [H]

Synonym: BMEA_B0569

Alternate gene names: 225686380

Gene position: 561277-559796 (Counterclockwise)

Preceding gene: 225686381

Following gene: 225686379

Centisome position: 47.34

GC content: 60.05

Gene sequence:

>1482_bases
ATGAGTGAACTGACCGCACTGACCATTGCCGAAGCGCGCGACAAGTTGAAAGCCAAGGCCATTACCGCGACCGAGCTGAC
GGACGCCTATCTTTCCGCTATCGATGCCGCCAATGACGCCATCAATGCCTATGTGGCCGTCACCCACGATCAGGCGCGTT
CCATGGCCAAGGCTTCGGATGAACGCATCGCGAAGGGCGAAGCGGGCGCATTGGAGGGCATTCCGCTCGGCGTGAAGGAC
CTGTTTGCAACCAAGGGCGTGCACACGCAGGCCTGCTCGCACATTCTGGACGGTTTCAAGCCGGAATATGAATCGACCGT
CACCGCCAATTTATGGGCTGATGGCGCGGTCATGCTCGGCAAGCTCAATATGGATGAGTTCGCCATGGGCTCCTCCAACG
AAACTTCCTATTACGGTCCGGTGAAAAATCCGTGGCGCGCCAAGGGTTCCAATGCCGATCTGGTGCCGGGCGGCTCGTCC
GGCGGTTCGGCTGCCGCCGTTGCCGCGCATCTTTGCGCGGGCGCGACTGCGACCGATACGGGCGGCTCGATCCGCCAGCC
TGCCGCCTTTACCGGTACGGTCGGCATCAAGCCAACCTATGGCCGTGTTTCGCGCTGGGGTACGGTGGCTTTCGCCTCGT
CGCTCGATCAGGCAGGCCCGATTGCGCGCGACGTGCGCGATGCTGCGATCCTTATGAAGTCCATGGCTTCGCTCGATCTC
AAGGACACAACTTCCGTTGACCTGCCGGTGCCGGATTATGAAGCAGCCCTCGGCAGGTCTGTGAAGGGCATGAAGATCGG
CATTCCAAGGGAATATCGTGTCGATGGCATGCCGGGCGAAATCGAGGAACTCTGGCAGAAGGGCATCCAGTATCTGAAGG
ATGCAGGTGCCGAGATTGTCGATATTTCGCTGCCGCATACCAAATATGCGCTGCCTGCTTATTATATCGTGGCGCCTGCG
GAAGCTTCGTCCAACCTCGCACGTTATGATGGCGTTCGTTATGGCCTGCGCGTGCCCGGCAAGGATATTGCCGACATGTA
CGAACAGACCCGCGCCGCAGGCTTCGGCAAGGAAGTGAAGCGCCGCATCATGATCGGCACCTATGTCCTGTCGGCGGGCT
ATTACGACGCTTATTACCTCCGCGCCCAGAAGGTGCGTACGCTGATCAAGAAGGATTTCGAGGACGTTTTCGCCAAGGGT
GTGGATGCTATCCTGACCCCGGCCACGCCTTCGGCGGCTTTCAGCCTTGCCGATGAAGTCCTCGCCAACGACCCGGTCAA
GATGTACCTGAACGATATCTTTACCGTCATCGTGAACATGGCGGGATTGCCGGGCATCGCGGTGCCTGCCGGGCTGAACG
GGCAGGGCCTGCCGCTTGGCCTCCAGCTTATCGGCCGCCCCTTCGAGGAAGAAACGCTGTTTCAGGCAGCCCATGTCATC
GAGCAGGCTGCCGGGCGTTTCACGCCAGCGAAGTGGTGGTAA

Upstream 100 bases:

>100_bases
ACGGAAGACAATTTCTTCGTGGTTCCGAAGGTCGTGGAGTAAGGCCTTCCAGCAACACACGAAAGCGCCCGCCGGAGCGG
CGCATTGATAGGATTTGACG

Downstream 100 bases:

>100_bases
AACCGGGGTTCTATCGGCCCAGGATTGCGTCGGCCTGAAAATCGGCCAATTCACGATGCAATGCCAAAAGATATCGAGCG
CCCCGCAATATCGGGGCGTG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSELTALTIAEARDKLKAKAITATELTDAYLSAIDAANDAINAYVAVTHDQARSMAKASDERIAKGEAGALEGIPLGVKD
LFATKGVHTQACSHILDGFKPEYESTVTANLWADGAVMLGKLNMDEFAMGSSNETSYYGPVKNPWRAKGSNADLVPGGSS
GGSAAAVAAHLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRVSRWGTVAFASSLDQAGPIARDVRDAAILMKSMASLDL
KDTTSVDLPVPDYEAALGRSVKGMKIGIPREYRVDGMPGEIEELWQKGIQYLKDAGAEIVDISLPHTKYALPAYYIVAPA
EASSNLARYDGVRYGLRVPGKDIADMYEQTRAAGFGKEVKRRIMIGTYVLSAGYYDAYYLRAQKVRTLIKKDFEDVFAKG
VDAILTPATPSAAFSLADEVLANDPVKMYLNDIFTVIVNMAGLPGIAVPAGLNGQGLPLGLQLIGRPFEEETLFQAAHVI
EQAAGRFTPAKWW

Sequences:

>Translated_493_residues
MSELTALTIAEARDKLKAKAITATELTDAYLSAIDAANDAINAYVAVTHDQARSMAKASDERIAKGEAGALEGIPLGVKD
LFATKGVHTQACSHILDGFKPEYESTVTANLWADGAVMLGKLNMDEFAMGSSNETSYYGPVKNPWRAKGSNADLVPGGSS
GGSAAAVAAHLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRVSRWGTVAFASSLDQAGPIARDVRDAAILMKSMASLDL
KDTTSVDLPVPDYEAALGRSVKGMKIGIPREYRVDGMPGEIEELWQKGIQYLKDAGAEIVDISLPHTKYALPAYYIVAPA
EASSNLARYDGVRYGLRVPGKDIADMYEQTRAAGFGKEVKRRIMIGTYVLSAGYYDAYYLRAQKVRTLIKKDFEDVFAKG
VDAILTPATPSAAFSLADEVLANDPVKMYLNDIFTVIVNMAGLPGIAVPAGLNGQGLPLGLQLIGRPFEEETLFQAAHVI
EQAAGRFTPAKWW
>Mature_492_residues
SELTALTIAEARDKLKAKAITATELTDAYLSAIDAANDAINAYVAVTHDQARSMAKASDERIAKGEAGALEGIPLGVKDL
FATKGVHTQACSHILDGFKPEYESTVTANLWADGAVMLGKLNMDEFAMGSSNETSYYGPVKNPWRAKGSNADLVPGGSSG
GSAAAVAAHLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRVSRWGTVAFASSLDQAGPIARDVRDAAILMKSMASLDLK
DTTSVDLPVPDYEAALGRSVKGMKIGIPREYRVDGMPGEIEELWQKGIQYLKDAGAEIVDISLPHTKYALPAYYIVAPAE
ASSNLARYDGVRYGLRVPGKDIADMYEQTRAAGFGKEVKRRIMIGTYVLSAGYYDAYYLRAQKVRTLIKKDFEDVFAKGV
DAILTPATPSAAFSLADEVLANDPVKMYLNDIFTVIVNMAGLPGIAVPAGLNGQGLPLGLQLIGRPFEEETLFQAAHVIE
QAAGRFTPAKWW

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=495, Percent_Identity=41.010101010101, Blast_Score=352, Evalue=5e-97,
Organism=Homo sapiens, GI195972892, Length=509, Percent_Identity=26.1296660117878, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI166795287, Length=258, Percent_Identity=24.4186046511628, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=444, Percent_Identity=36.036036036036, Blast_Score=271, Evalue=8e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=534, Percent_Identity=26.7790262172285, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17538252, Length=269, Percent_Identity=24.5353159851301, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=542, Percent_Identity=22.140221402214, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17556264, Length=199, Percent_Identity=29.1457286432161, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=205, Percent_Identity=33.6585365853659, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=205, Percent_Identity=33.6585365853659, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538254, Length=536, Percent_Identity=22.3880597014925, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=432, Percent_Identity=35.4166666666667, Blast_Score=253, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6319685, Length=431, Percent_Identity=27.6102088167053, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320448, Length=247, Percent_Identity=28.3400809716599, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648113, Length=489, Percent_Identity=38.0368098159509, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24652985, Length=500, Percent_Identity=24, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19922090, Length=500, Percent_Identity=24, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24652981, Length=500, Percent_Identity=24, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24652983, Length=500, Percent_Identity=24, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24644968, Length=507, Percent_Identity=23.6686390532544, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161078093, Length=531, Percent_Identity=25.2354048964218, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356731, Length=515, Percent_Identity=25.631067961165, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24648435, Length=515, Percent_Identity=24.0776699029126, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648437, Length=515, Percent_Identity=24.0776699029126, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45550774, Length=248, Percent_Identity=29.8387096774194, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24648441, Length=443, Percent_Identity=23.9277652370203, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648439, Length=443, Percent_Identity=23.9277652370203, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52489; Mature: 52358

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELTALTIAEARDKLKAKAITATELTDAYLSAIDAANDAINAYVAVTHDQARSMAKASD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHH
ERIAKGEAGALEGIPLGVKDLFATKGVHTQACSHILDGFKPEYESTVTANLWADGAVMLG
HHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEE
KLNMDEFAMGSSNETSYYGPVKNPWRAKGSNADLVPGGSSGGSAAAVAAHLCAGATATDT
ECCHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
GGSIRQPAAFTGTVGIKPTYGRVSRWGTVAFASSLDQAGPIARDVRDAAILMKSMASLDL
CCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
KDTTSVDLPVPDYEAALGRSVKGMKIGIPREYRVDGMPGEIEELWQKGIQYLKDAGAEIV
CCCCCCCCCCCCHHHHHCCCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHHHHCCCEEE
DISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVPGKDIADMYEQTRAAGFGKEVK
EEECCCCCCCCCEEEEEECCCCCCCHHHHCCCEECCCCCCHHHHHHHHHHHHHCCCHHHH
RRIMIGTYVLSAGYYDAYYLRAQKVRTLIKKDFEDVFAKGVDAILTPATPSAAFSLADEV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHH
LANDPVKMYLNDIFTVIVNMAGLPGIAVPAGLNGQGLPLGLQLIGRPFEEETLFQAAHVI
HHCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHH
EQAAGRFTPAKWW
HHHHCCCCCCCCC
>Mature Secondary Structure 
SELTALTIAEARDKLKAKAITATELTDAYLSAIDAANDAINAYVAVTHDQARSMAKASD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHH
ERIAKGEAGALEGIPLGVKDLFATKGVHTQACSHILDGFKPEYESTVTANLWADGAVMLG
HHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEE
KLNMDEFAMGSSNETSYYGPVKNPWRAKGSNADLVPGGSSGGSAAAVAAHLCAGATATDT
ECCHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
GGSIRQPAAFTGTVGIKPTYGRVSRWGTVAFASSLDQAGPIARDVRDAAILMKSMASLDL
CCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
KDTTSVDLPVPDYEAALGRSVKGMKIGIPREYRVDGMPGEIEELWQKGIQYLKDAGAEIV
CCCCCCCCCCCCHHHHHCCCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHHHHCCCEEE
DISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVPGKDIADMYEQTRAAGFGKEVK
EEECCCCCCCCCEEEEEECCCCCCCHHHHCCCEECCCCCCHHHHHHHHHHHHHCCCHHHH
RRIMIGTYVLSAGYYDAYYLRAQKVRTLIKKDFEDVFAKGVDAILTPATPSAAFSLADEV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHH
LANDPVKMYLNDIFTVIVNMAGLPGIAVPAGLNGQGLPLGLQLIGRPFEEETLFQAAHVI
HHCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHH
EQAAGRFTPAKWW
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA