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The map label for this gene is phaF [H]

Identifier: 225686300

GI number: 225686300

Start: 465745

End: 466026

Strand: Direct

Name: phaF [H]

Synonym: BMEA_B0479

Alternate gene names: 225686300

Gene position: 465745-466026 (Clockwise)

Preceding gene: 225686299

Following gene: 225686301

Centisome position: 39.29

GC content: 60.64

Gene sequence:

>282_bases
ATGAGCGCAGTTCTCATCTTCTGGGCGCTGCTTGCCGCTCAGGTCATGCTCGTTGCAGCCATGGGCTGCGCGGTCTATCG
CCTGCTGGTTGGTCCGCGCGCGCAGGATCGTATTCTGGCGACCGATGCGCTTTATCTCGACGCGCTCCTGCTTCTGCTCA
CTTTCGGCATCCGCACGGGCAGCATCATCTATTTCGAGGCCGCGCTCATCATCGCCCTTCTGGGTTTCGTTGCCACGGTG
GCGCTGTCCAAATTCCTGATGCGCGGCGAGGTGATCGAATGA

Upstream 100 bases:

>100_bases
AAGATGCCGAAACCTGGCGACATCTTATCAAGGAGCGCTACGAACGGCCGCTGATCGAGATTTTCGGTGTGGTGGATATC
GTTGAAGGGGAGAAATCGGC

Downstream 100 bases:

>100_bases
TAGAGGCTGCCGATTTTCCGCTCTGGGCCGCGATCCTTGTTTCGTTTCTTCTGGTGGCGGGAGCCTTTCTCACGCTCGTC
GGCTGCATCGGGCTCGTGCG

Product: putative monovalent cation/H+ antiporter subunit F

Products: NA

Alternate protein names: pH adaptation potassium efflux system protein F; Pha system subunit F [H]

Number of amino acids: Translated: 93; Mature: 92

Protein sequence:

>93_residues
MSAVLIFWALLAAQVMLVAAMGCAVYRLLVGPRAQDRILATDALYLDALLLLLTFGIRTGSIIYFEAALIIALLGFVATV
ALSKFLMRGEVIE

Sequences:

>Translated_93_residues
MSAVLIFWALLAAQVMLVAAMGCAVYRLLVGPRAQDRILATDALYLDALLLLLTFGIRTGSIIYFEAALIIALLGFVATV
ALSKFLMRGEVIE
>Mature_92_residues
SAVLIFWALLAAQVMLVAAMGCAVYRLLVGPRAQDRILATDALYLDALLLLLTFGIRTGSIIYFEAALIIALLGFVATVA
LSKFLMRGEVIE

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG2212

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhF subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit F family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007208 [H]

Pfam domain/function: PF04066 MrpF_PhaF [H]

EC number: NA

Molecular weight: Translated: 10046; Mature: 9915

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVLIFWALLAAQVMLVAAMGCAVYRLLVGPRAQDRILATDALYLDALLLLLTFGIRTG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
SIIYFEAALIIALLGFVATVALSKFLMRGEVIE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SAVLIFWALLAAQVMLVAAMGCAVYRLLVGPRAQDRILATDALYLDALLLLLTFGIRTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
SIIYFEAALIIALLGFVATVALSKFLMRGEVIE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]