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The map label for this gene is ynfL [H]

Identifier: 225686251

GI number: 225686251

Start: 414527

End: 415414

Strand: Direct

Name: ynfL [H]

Synonym: BMEA_B0424

Alternate gene names: 225686251

Gene position: 414527-415414 (Clockwise)

Preceding gene: 225686249

Following gene: 225686252

Centisome position: 34.97

GC content: 58.78

Gene sequence:

>888_bases
ATGGAATTGCGCCATCTGCGTTATTTTCTGGCCGTGGCGGAAGAGGGAAACTTCACGCGCGCGGCGGCAAAGCTTGGCAT
CGGCCAACCCCCGCTCAGCCAGCAAATTCGTGATCTTGAAAACGAGATTGGCGTAATGCTGTTTCATCGCGTGCCGCATG
GTGCCGAACTCACGGCGGCGGGAGCGGCTTTTCTCGAAGAGGCAACAGCCACGATTGCCTCATCTGAAAAAGCAAAACTG
GCAGCGCAGCGTGCCGCACGCGGGGAAAGCGGGCGGCTTTCGCTGGGGTTCACCGCATCGTCGGCATTCAACCCCATCGT
TAGTGGCGCGATCCGGCATTTCCGCGCGAAATGGCCTGAGGTGGAACTGTTTCTGACGGAGATGAACACCAATCTCCTCA
TCGAGCGGCTGGTGCGTGGAGAGCTGGATACAGCCTTTATCCGTCCGGGGCTGGAAGACCCGGCCAATGTGCGGATGAAG
CGCCTTGCCGATGAACCGATGCTTGTTGCACTGCCCAGCCACCACAGGCTGGCGGTTTATGAACGGCTGCCGCTTTCCGC
ACTGGCGGGGGAGCCCTTCGTTCTCTTTCCGCGTATCGTGGGTCTCAGTCTTTATGACGATATTGCCGCAGCCTGCCGCA
TGGCCGGTTTTGAGATGAATGTCACGCAGGAAGCACCGCAAATGCCTTCCGTGGTCAATCTGGTTGCGGCCAATCTGGGG
GTGTCTATCGTGCCAGCGGCAATTGCGCAGATCAGGCTGGACGGCGTGACCTACAGGCCTATCGAGGGTGAACCGCTCGT
TGCGCGTCTGGCGCTTGCATCCATGAAAAGCCTGCGCTCGCCTGTCCACGATAATCTGATAAGCCTTCTGCCGAGGGAAT
GCATATAA

Upstream 100 bases:

>100_bases
GGCCTGCCTTATGTAACCTCCCGTGTTTTTAATCTAGCCAGATGAGCTTCATTTGTCCAATATATGAAACAGTTAACTGT
GATAGCTTTTGGATATACCA

Downstream 100 bases:

>100_bases
TATGGAGACCACAGGTGCGGTCTTGCGAAGCGCTTGCCTCTGTTCCCATCTATAAGTTTATTGAAATGACAGGGAGATGC
CCGGATGAAATATCAAAAAA

Product: HTH-type transcriptional regulator BudR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MELRHLRYFLAVAEEGNFTRAAAKLGIGQPPLSQQIRDLENEIGVMLFHRVPHGAELTAAGAAFLEEATATIASSEKAKL
AAQRAARGESGRLSLGFTASSAFNPIVSGAIRHFRAKWPEVELFLTEMNTNLLIERLVRGELDTAFIRPGLEDPANVRMK
RLADEPMLVALPSHHRLAVYERLPLSALAGEPFVLFPRIVGLSLYDDIAAACRMAGFEMNVTQEAPQMPSVVNLVAANLG
VSIVPAAIAQIRLDGVTYRPIEGEPLVARLALASMKSLRSPVHDNLISLLPRECI

Sequences:

>Translated_295_residues
MELRHLRYFLAVAEEGNFTRAAAKLGIGQPPLSQQIRDLENEIGVMLFHRVPHGAELTAAGAAFLEEATATIASSEKAKL
AAQRAARGESGRLSLGFTASSAFNPIVSGAIRHFRAKWPEVELFLTEMNTNLLIERLVRGELDTAFIRPGLEDPANVRMK
RLADEPMLVALPSHHRLAVYERLPLSALAGEPFVLFPRIVGLSLYDDIAAACRMAGFEMNVTQEAPQMPSVVNLVAANLG
VSIVPAAIAQIRLDGVTYRPIEGEPLVARLALASMKSLRSPVHDNLISLLPRECI
>Mature_295_residues
MELRHLRYFLAVAEEGNFTRAAAKLGIGQPPLSQQIRDLENEIGVMLFHRVPHGAELTAAGAAFLEEATATIASSEKAKL
AAQRAARGESGRLSLGFTASSAFNPIVSGAIRHFRAKWPEVELFLTEMNTNLLIERLVRGELDTAFIRPGLEDPANVRMK
RLADEPMLVALPSHHRLAVYERLPLSALAGEPFVLFPRIVGLSLYDDIAAACRMAGFEMNVTQEAPQMPSVVNLVAANLG
VSIVPAAIAQIRLDGVTYRPIEGEPLVARLALASMKSLRSPVHDNLISLLPRECI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=276, Percent_Identity=40.9420289855072, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1788887, Length=290, Percent_Identity=36.2068965517241, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI2367136, Length=261, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1790399, Length=268, Percent_Identity=32.089552238806, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI157672245, Length=256, Percent_Identity=33.984375, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788297, Length=147, Percent_Identity=35.3741496598639, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788748, Length=245, Percent_Identity=25.3061224489796, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI145693105, Length=266, Percent_Identity=24.812030075188, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081904, Length=201, Percent_Identity=30.3482587064677, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082132, Length=251, Percent_Identity=28.6852589641434, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790262, Length=298, Percent_Identity=26.510067114094, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789204, Length=247, Percent_Identity=27.1255060728745, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787601, Length=143, Percent_Identity=27.972027972028, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787530, Length=246, Percent_Identity=23.1707317073171, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787806, Length=147, Percent_Identity=31.9727891156463, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32101; Mature: 32101

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRYFLAVAEEGNFTRAAAKLGIGQPPLSQQIRDLENEIGVMLFHRVPHGAELTAA
CCHHHHEEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCEEEEECCCCCCCHHHH
GAAFLEEATATIASSEKAKLAAQRAARGESGRLSLGFTASSAFNPIVSGAIRHFRAKWPE
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCC
VELFLTEMNTNLLIERLVRGELDTAFIRPGLEDPANVRMKRLADEPMLVALPSHHRLAVY
EEEEEEECCHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCEEEHH
ERLPLSALAGEPFVLFPRIVGLSLYDDIAAACRMAGFEMNVTQEAPQMPSVVNLVAANLG
HHCCHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
VSIVPAAIAQIRLDGVTYRPIEGEPLVARLALASMKSLRSPVHDNLISLLPRECI
CHHHHHHHHHHEECCEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELRHLRYFLAVAEEGNFTRAAAKLGIGQPPLSQQIRDLENEIGVMLFHRVPHGAELTAA
CCHHHHEEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCEEEEECCCCCCCHHHH
GAAFLEEATATIASSEKAKLAAQRAARGESGRLSLGFTASSAFNPIVSGAIRHFRAKWPE
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCC
VELFLTEMNTNLLIERLVRGELDTAFIRPGLEDPANVRMKRLADEPMLVALPSHHRLAVY
EEEEEEECCHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCEEEHH
ERLPLSALAGEPFVLFPRIVGLSLYDDIAAACRMAGFEMNVTQEAPQMPSVVNLVAANLG
HHCCHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
VSIVPAAIAQIRLDGVTYRPIEGEPLVARLALASMKSLRSPVHDNLISLLPRECI
CHHHHHHHHHHEECCEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]