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The map label for this gene is wbjE [H]

Identifier: 225686229

GI number: 225686229

Start: 386103

End: 387443

Strand: Direct

Name: wbjE [H]

Synonym: BMEA_B0398

Alternate gene names: 225686229

Gene position: 386103-387443 (Clockwise)

Preceding gene: 225686227

Following gene: 225686230

Centisome position: 32.57

GC content: 58.76

Gene sequence:

>1341_bases
GTGCAGCCCATGCCTTTGTGTTTCTTGCACTGCCAGCCGCCCTGTTGCGATCCTCACCGCGAGGGGATCCGAGCAATAGG
AAGCAAACTCATGCGTGTGGTGCTTGCCAATCGTTACTTCTACCCGGACCAGTCGGCGACAAGCCGCATGGTGACAAGCC
TTGCCCATGCTCTGGTGTGTGAGGGCATTGAAACATCTGTGCTTGCAAGCCGCAGCTATCACGACAGGCGCAAGGAAACC
CTGGCCGCGCGAGAGACGATCGACGGTATCGAAGTACATCGCATCTGGACCTCCGGTTTCGGGCGCGGCAGGCTTGCCGG
GCGGGCCGTCGATTACGCTACCTTTCACCTTTCGGCGGCGGCATGGTTTGCCGCCAATGCCCGCAAGGACGATCTCTGCG
TTATGTGCACCGATCCGCCGCTCCTGTCTGTTTCGGCGGCTTTGCCCATCCGTTTTCGCCGCGCAAGGCTCGTCAACTGG
GTGATGGATCTTTTCCCTGAAACTGCCATGGAACTGGGTCTCATCAGGCCAGATACCGTTTCCGGCAGGCTGGCTATGGC
ATTGCGCGACTGGTCCATGCGCCAGTCTGCCCTGACCATATGCCCCATAGACCGCATGGCACACTATCTGGCGAAGAGGG
ATATTCCGGCAGAAAGCCTTGGTGTCGTTCATCATTGGGCCGACCGCAACGAGATTGTGCCTGTCGAGCCGGTGGAAAAT
CCGTTGCGGCGCGCCTGGGGGCTGGGACGCAAATTCGTCATCGGCTATTCCGGCAATTTTGGCCGTGCCCATGAATTCCG
AACAATACTGGATGCTGCCGAATGGCTGCGGCACAGGAAAAGCATCGTCTTCCTGATGATAGGGGAAGGCCAGCAGCGCG
GCTTCGTGGAAAGCGAAGTGAAGCGTCGTGGCCTTGCCAATGTCATGATGAAACCGTTCCAGCCGGTCGAAAAACTCTCG
GAAAGCCTTGGCGCGGCCAATGTGCATCTGGTTTCGCTGAAGTCGGAACTGGAGCATTGCATCGTGCCGAGCAAGTTTTA
CGGTGTTCTGGCTGCCGCGCGCCCCACGATCTTCATTGGTGATCCTGATGGCGAGATTGGCTCCGTTGTGCGGGATTTCC
AATGCGGAATGGCGTTGAGACCGGGCGATGTCGATGCGCTGGTGGGGGCAATCCTGCATCTGCGGCATTCGCCGGTCAGT
TGCACCACCATGGGGAATAATGCGCGCTATCTCATGGAAACCGCCTATTCCCGTGAATATGGCGCGGCCATGTGGCAAGC
CGCTATTGGACGGCTGGAAGAAAAGGCAGCCCCCTCGGTCATGCCTGTCCTCGACCGGTAA

Upstream 100 bases:

>100_bases
ACGGCAATTCCAGCCCCGGCGCTCCCACTTCGGGTGAGGGAGAAGATGCGCCCGGTAAAGCCCTTTTCACCCAAAGGTGT
AGGCGGTTCGCCCGCTGTCG

Downstream 100 bases:

>100_bases
TTCCAACCGGGAAAAATGTGATGGATGGTTCCGTGGTCGTCAGCCAGTTGGGCGCGCGGATGCACTATGCGGTGCCTCGC
ATTTTTGCCAGCCGCGAAAG

Product: putative glycosyltransferase wbjE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MQPMPLCFLHCQPPCCDPHREGIRAIGSKLMRVVLANRYFYPDQSATSRMVTSLAHALVCEGIETSVLASRSYHDRRKET
LAARETIDGIEVHRIWTSGFGRGRLAGRAVDYATFHLSAAAWFAANARKDDLCVMCTDPPLLSVSAALPIRFRRARLVNW
VMDLFPETAMELGLIRPDTVSGRLAMALRDWSMRQSALTICPIDRMAHYLAKRDIPAESLGVVHHWADRNEIVPVEPVEN
PLRRAWGLGRKFVIGYSGNFGRAHEFRTILDAAEWLRHRKSIVFLMIGEGQQRGFVESEVKRRGLANVMMKPFQPVEKLS
ESLGAANVHLVSLKSELEHCIVPSKFYGVLAAARPTIFIGDPDGEIGSVVRDFQCGMALRPGDVDALVGAILHLRHSPVS
CTTMGNNARYLMETAYSREYGAAMWQAAIGRLEEKAAPSVMPVLDR

Sequences:

>Translated_446_residues
MQPMPLCFLHCQPPCCDPHREGIRAIGSKLMRVVLANRYFYPDQSATSRMVTSLAHALVCEGIETSVLASRSYHDRRKET
LAARETIDGIEVHRIWTSGFGRGRLAGRAVDYATFHLSAAAWFAANARKDDLCVMCTDPPLLSVSAALPIRFRRARLVNW
VMDLFPETAMELGLIRPDTVSGRLAMALRDWSMRQSALTICPIDRMAHYLAKRDIPAESLGVVHHWADRNEIVPVEPVEN
PLRRAWGLGRKFVIGYSGNFGRAHEFRTILDAAEWLRHRKSIVFLMIGEGQQRGFVESEVKRRGLANVMMKPFQPVEKLS
ESLGAANVHLVSLKSELEHCIVPSKFYGVLAAARPTIFIGDPDGEIGSVVRDFQCGMALRPGDVDALVGAILHLRHSPVS
CTTMGNNARYLMETAYSREYGAAMWQAAIGRLEEKAAPSVMPVLDR
>Mature_446_residues
MQPMPLCFLHCQPPCCDPHREGIRAIGSKLMRVVLANRYFYPDQSATSRMVTSLAHALVCEGIETSVLASRSYHDRRKET
LAARETIDGIEVHRIWTSGFGRGRLAGRAVDYATFHLSAAAWFAANARKDDLCVMCTDPPLLSVSAALPIRFRRARLVNW
VMDLFPETAMELGLIRPDTVSGRLAMALRDWSMRQSALTICPIDRMAHYLAKRDIPAESLGVVHHWADRNEIVPVEPVEN
PLRRAWGLGRKFVIGYSGNFGRAHEFRTILDAAEWLRHRKSIVFLMIGEGQQRGFVESEVKRRGLANVMMKPFQPVEKLS
ESLGAANVHLVSLKSELEHCIVPSKFYGVLAAARPTIFIGDPDGEIGSVVRDFQCGMALRPGDVDALVGAILHLRHSPVS
CTTMGNNARYLMETAYSREYGAAMWQAAIGRLEEKAAPSVMPVLDR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788363, Length=189, Percent_Identity=25.9259259259259, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 49718; Mature: 49718

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPMPLCFLHCQPPCCDPHREGIRAIGSKLMRVVLANRYFYPDQSATSRMVTSLAHALVC
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
EGIETSVLASRSYHDRRKETLAARETIDGIEVHRIWTSGFGRGRLAGRAVDYATFHLSAA
CCCHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEHHHHEEHHHH
AWFAANARKDDLCVMCTDPPLLSVSAALPIRFRRARLVNWVMDLFPETAMELGLIRPDTV
HHHHCCCCCCCEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SGRLAMALRDWSMRQSALTICPIDRMAHYLAKRDIPAESLGVVHHWADRNEIVPVEPVEN
CCCEEEEHHHHHHHHCCEEEECHHHHHHHHHHCCCCHHHHHHHHEECCCCCEECCCCCHH
PLRRAWGLGRKFVIGYSGNFGRAHEFRTILDAAEWLRHRKSIVFLMIGEGQQRGFVESEV
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHH
KRRGLANVMMKPFQPVEKLSESLGAANVHLVSLKSELEHCIVPSKFYGVLAAARPTIFIG
HHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCHHHHHHHHHCCCEEEEE
DPDGEIGSVVRDFQCGMALRPGDVDALVGAILHLRHSPVSCTTMGNNARYLMETAYSREY
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHH
GAAMWQAAIGRLEEKAAPSVMPVLDR
HHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQPMPLCFLHCQPPCCDPHREGIRAIGSKLMRVVLANRYFYPDQSATSRMVTSLAHALVC
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
EGIETSVLASRSYHDRRKETLAARETIDGIEVHRIWTSGFGRGRLAGRAVDYATFHLSAA
CCCHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEHHHHEEHHHH
AWFAANARKDDLCVMCTDPPLLSVSAALPIRFRRARLVNWVMDLFPETAMELGLIRPDTV
HHHHCCCCCCCEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SGRLAMALRDWSMRQSALTICPIDRMAHYLAKRDIPAESLGVVHHWADRNEIVPVEPVEN
CCCEEEEHHHHHHHHCCEEEECHHHHHHHHHHCCCCHHHHHHHHEECCCCCEECCCCCHH
PLRRAWGLGRKFVIGYSGNFGRAHEFRTILDAAEWLRHRKSIVFLMIGEGQQRGFVESEV
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHH
KRRGLANVMMKPFQPVEKLSESLGAANVHLVSLKSELEHCIVPSKFYGVLAAARPTIFIG
HHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCHHHHHHHHHCCCEEEEE
DPDGEIGSVVRDFQCGMALRPGDVDALVGAILHLRHSPVSCTTMGNNARYLMETAYSREY
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHH
GAAMWQAAIGRLEEKAAPSVMPVLDR
HHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400585; 10828401; 12057956 [H]