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The map label for this gene is livJ [C]

Identifier: 225686212

GI number: 225686212

Start: 363358

End: 364521

Strand: Direct

Name: livJ [C]

Synonym: BMEA_B0379

Alternate gene names: 225686212

Gene position: 363358-364521 (Clockwise)

Preceding gene: 225686211

Following gene: 225686213

Centisome position: 30.65

GC content: 55.76

Gene sequence:

>1164_bases
TTGCTGGCAGGCGCCGCGCCTGCCTCGGCGGATATCAAGATGGGGTCGCTTTACCCGTTCAGCGGTCCGCTGGCGCTTCT
GGGAGATGAAAGCGCGCGCGGTCTTGAAATAGCCGTGGAAGAGATCAACGCCAAGGGTGGCGTACAGGGCGAAAAAATCG
TTCTGGTGCGAGGCGACGCGGTTGATAACAATCAGGCGATCGGTGAAGCGCGTCGCCTGATCTCCGTTGAGAATGTTGCC
GGGATTTTCGGGTCATTTTCATCCGGTCGTGCGGTTGCCGCCAGCCAGGTGAGCGAACTGGCGGGCGTGCCTTATTTCGA
GCTAGGGGCGGTGGCCGACGAGATCACCGATCGGGGACTGGAAAACGTCTACCGCGCCAATCCCTATGCCCGTGATTTTG
CGCAGATGATTGTGGAGATGCTCCAGAAGAAGATCGCGCCAAAGCTGGGCAGGGATTCGAAAGACCTCAAGATCGCGGTC
ATCTATGAGGATTCGAGCTATGGCACCTCCGTCGCCAAGCATGAGGAGACTTTCCTGAAGGAAGCTGGCCTCAACATGGT
GCTGTCGCAATCCTATCCGGGCAACACCGTCGATATGTCGTCTCTGGTTCTCGACCTGAAGAGCGCCGGTGCGGATGTGG
TTCTGCAAACCTCCTATCAAAGCGATTCAGTCCTGTTTCTGCAGCAGGCTAATGAAGGTGGCTATAAGCCATCCGCAATC
GTCGGTGCCGGCGGTGGATATTCGCTTCAGCCGACTGCCGATGCCGTGGGGCATGATGTGATTGAAGCAGCCTATGATGT
CGATTTCACGCAATTCGCCGTCAATACGAGCTTTACGCCCGGCCTTGAGGAGTTTGTCGAAGCCTACAAGAAAAAATATG
GTGAAACACCGCGTTCAGGCCATTCGCTTACCAATTATGTCGGTGCGAAAGTCATTCTCGAAGCCTTGAACAAGGTTAAG
GGATTTGATGCGGCTGCGGTCAAGCAGGCCTTGAGCGCAGTCGATATTGAAGCGGGCAAGAATGCGATGGGTTACGGGTT
CAAGTTTGACCAGAACAACCAGAATGAGCGTGCATCCATGATGGGGATGCAATGGCAGGATGGCAAACTGGTCACTGTCT
ACCCGGACGCCGCCGCCATATCGGAAATCCGGTTGCCGCAGTAA

Upstream 100 bases:

>100_bases
ATGGTCTGACATTTGAAAAATTTCCAAGAAAGGCTTTGGGGGCCTGTTTCAAGAGGAGGGAAAATTGGAAAAACATCTTA
TCGCATTATCTGTTGCAGCC

Downstream 100 bases:

>100_bases
CAGTCGTCACCGAGGTTGGGCAGTCTGCCCAGCCTCCCTGAAGCCTTAAAGGAACCAGATCGATGAAATTCAAAGCCGCT
GTTCTTGACCGCCATGGTGT

Product: Leu/Ile/Val-binding protein family protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MLAGAAPASADIKMGSLYPFSGPLALLGDESARGLEIAVEEINAKGGVQGEKIVLVRGDAVDNNQAIGEARRLISVENVA
GIFGSFSSGRAVAASQVSELAGVPYFELGAVADEITDRGLENVYRANPYARDFAQMIVEMLQKKIAPKLGRDSKDLKIAV
IYEDSSYGTSVAKHEETFLKEAGLNMVLSQSYPGNTVDMSSLVLDLKSAGADVVLQTSYQSDSVLFLQQANEGGYKPSAI
VGAGGGYSLQPTADAVGHDVIEAAYDVDFTQFAVNTSFTPGLEEFVEAYKKKYGETPRSGHSLTNYVGAKVILEALNKVK
GFDAAAVKQALSAVDIEAGKNAMGYGFKFDQNNQNERASMMGMQWQDGKLVTVYPDAAAISEIRLPQ

Sequences:

>Translated_387_residues
MLAGAAPASADIKMGSLYPFSGPLALLGDESARGLEIAVEEINAKGGVQGEKIVLVRGDAVDNNQAIGEARRLISVENVA
GIFGSFSSGRAVAASQVSELAGVPYFELGAVADEITDRGLENVYRANPYARDFAQMIVEMLQKKIAPKLGRDSKDLKIAV
IYEDSSYGTSVAKHEETFLKEAGLNMVLSQSYPGNTVDMSSLVLDLKSAGADVVLQTSYQSDSVLFLQQANEGGYKPSAI
VGAGGGYSLQPTADAVGHDVIEAAYDVDFTQFAVNTSFTPGLEEFVEAYKKKYGETPRSGHSLTNYVGAKVILEALNKVK
GFDAAAVKQALSAVDIEAGKNAMGYGFKFDQNNQNERASMMGMQWQDGKLVTVYPDAAAISEIRLPQ
>Mature_387_residues
MLAGAAPASADIKMGSLYPFSGPLALLGDESARGLEIAVEEINAKGGVQGEKIVLVRGDAVDNNQAIGEARRLISVENVA
GIFGSFSSGRAVAASQVSELAGVPYFELGAVADEITDRGLENVYRANPYARDFAQMIVEMLQKKIAPKLGRDSKDLKIAV
IYEDSSYGTSVAKHEETFLKEAGLNMVLSQSYPGNTVDMSSLVLDLKSAGADVVLQTSYQSDSVLFLQQANEGGYKPSAI
VGAGGGYSLQPTADAVGHDVIEAAYDVDFTQFAVNTSFTPGLEEFVEAYKKKYGETPRSGHSLTNYVGAKVILEALNKVK
GFDAAAVKQALSAVDIEAGKNAMGYGFKFDQNNQNERASMMGMQWQDGKLVTVYPDAAAISEIRLPQ

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=227, Percent_Identity=29.5154185022026, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789867, Length=227, Percent_Identity=28.6343612334802, Blast_Score=91, Evalue=9e-20,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41155; Mature: 41155

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAGAAPASADIKMGSLYPFSGPLALLGDESARGLEIAVEEINAKGGVQGEKIVLVRGDA
CCCCCCCCCCCEEECCCCCCCCCEEEECCCCCCCCEEEEEHHCCCCCCCCCEEEEEECCC
VDNNQAIGEARRLISVENVAGIFGSFSSGRAVAASQVSELAGVPYFELGAVADEITDRGL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ENVYRANPYARDFAQMIVEMLQKKIAPKLGRDSKDLKIAVIYEDSSYGTSVAKHEETFLK
HHHHHCCCHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
EAGLNMVLSQSYPGNTVDMSSLVLDLKSAGADVVLQTSYQSDSVLFLQQANEGGYKPSAI
HCCCCEEEECCCCCCCEEHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCCCCCCEE
VGAGGGYSLQPTADAVGHDVIEAAYDVDFTQFAVNTSFTPGLEEFVEAYKKKYGETPRSG
EECCCCCCCCCCHHHHHHHHHHHHHCCCHHHEEECCCCCCCHHHHHHHHHHHHCCCCCCC
HSLTNYVGAKVILEALNKVKGFDAAAVKQALSAVDIEAGKNAMGYGFKFDQNNQNERASM
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHH
MGMQWQDGKLVTVYPDAAAISEIRLPQ
CCCEECCCEEEEECCCHHHHHHCCCCC
>Mature Secondary Structure
MLAGAAPASADIKMGSLYPFSGPLALLGDESARGLEIAVEEINAKGGVQGEKIVLVRGDA
CCCCCCCCCCCEEECCCCCCCCCEEEECCCCCCCCEEEEEHHCCCCCCCCCEEEEEECCC
VDNNQAIGEARRLISVENVAGIFGSFSSGRAVAASQVSELAGVPYFELGAVADEITDRGL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ENVYRANPYARDFAQMIVEMLQKKIAPKLGRDSKDLKIAVIYEDSSYGTSVAKHEETFLK
HHHHHCCCHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
EAGLNMVLSQSYPGNTVDMSSLVLDLKSAGADVVLQTSYQSDSVLFLQQANEGGYKPSAI
HCCCCEEEECCCCCCCEEHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCCCCCCEE
VGAGGGYSLQPTADAVGHDVIEAAYDVDFTQFAVNTSFTPGLEEFVEAYKKKYGETPRSG
EECCCCCCCCCCHHHHHHHHHHHHHCCCHHHEEECCCCCCCHHHHHHHHHHHHCCCCCCC
HSLTNYVGAKVILEALNKVKGFDAAAVKQALSAVDIEAGKNAMGYGFKFDQNNQNERASM
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHH
MGMQWQDGKLVTVYPDAAAISEIRLPQ
CCCEECCCEEEEECCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA