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The map label for this gene is glnA [H]

Identifier: 225631022

GI number: 225631022

Start: 1444962

End: 1445699

Strand: Reverse

Name: glnA [H]

Synonym: WRi_013520

Alternate gene names: 225631022

Gene position: 1445699-1444962 (Counterclockwise)

Preceding gene: NA

Following gene: 225631020

Centisome position: 99.99

GC content: 34.28

Gene sequence:

>738_bases
ATGATTATATTAAGTAATTTGAGTTGTTATGCTGTATTCGGTATTGAACTAGAGTTTTACATTGAAGGAATAGAAAAAGA
ATACTTATTTCTGAATAGCATCAAAAATAAAATAGCATCTCTTGAATTTTCTTGCGAGAAGGAGAATTCTACACATCAGT
ATGAGATAAAAAGTGGTTGTTATACGAACTCCTACAATTTAATTAAGCATTTTGAGTTAGCAAAACAATCACTTACTGAA
ACAGCACAAAAACTAGGTGGGAATGTTTCTTTTAAAGCAAAGCCTTATTTGGATAGAGCAGGCAGTGCATTAAATGTGCA
TGTTAATCTCGTGAATTCAAATAACGGTAACTTATTTTATATCAATGAGCAAAAGTACAGTGACTATCTGATTCATAGTA
TTGGTGGATTATGTGCAATGATGAAAAAACATATGTTGTTTTTTGCTCCAAATGATGATTCATATTTAAGATTTCAGTAT
CCGGATATTCACACTCCAACTACGATTAGTTGGGGAGTAAATAACAGAACTGCTGCGATAAGAATTCCCTATTTTGGCAA
CGATTTCAAAAGATGCCGCCTAGAGCACCGCGTTCCCGGAGCAGATTGTGACCTTGAAAAAGTACTCACAGCAATAATTG
AAGGTATGGTTTTTGGTATAGAGAACAAAATTGCTCCGCCAAATAGGGTCTACGGCATTGCATCTGATCCTCAATATGAG
ATGGAGAGGTTAATATAA

Upstream 100 bases:

>100_bases
TAATACATTATGTATAAATAAGATGTTAAATTTACTGGCAAATAAGTAAAGTATTCATATAAATATAGCTAACCTAATAT
AGATTTACTGAAGCAAATTT

Downstream 100 bases:

>100_bases
CAACTATCTATCAAATCCTAATCCGTTAAACCCCTTTTGGGTAATTGGCTGTTTTAGCATTTTTTTTACATCATTATCTA
CATTTTTGCACTGTAATAGC

Product: glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MIILSNLSCYAVFGIELEFYIEGIEKEYLFLNSIKNKIASLEFSCEKENSTHQYEIKSGCYTNSYNLIKHFELAKQSLTE
TAQKLGGNVSFKAKPYLDRAGSALNVHVNLVNSNNGNLFYINEQKYSDYLIHSIGGLCAMMKKHMLFFAPNDDSYLRFQY
PDIHTPTTISWGVNNRTAAIRIPYFGNDFKRCRLEHRVPGADCDLEKVLTAIIEGMVFGIENKIAPPNRVYGIASDPQYE
MERLI

Sequences:

>Translated_245_residues
MIILSNLSCYAVFGIELEFYIEGIEKEYLFLNSIKNKIASLEFSCEKENSTHQYEIKSGCYTNSYNLIKHFELAKQSLTE
TAQKLGGNVSFKAKPYLDRAGSALNVHVNLVNSNNGNLFYINEQKYSDYLIHSIGGLCAMMKKHMLFFAPNDDSYLRFQY
PDIHTPTTISWGVNNRTAAIRIPYFGNDFKRCRLEHRVPGADCDLEKVLTAIIEGMVFGIENKIAPPNRVYGIASDPQYE
MERLI
>Mature_245_residues
MIILSNLSCYAVFGIELEFYIEGIEKEYLFLNSIKNKIASLEFSCEKENSTHQYEIKSGCYTNSYNLIKHFELAKQSLTE
TAQKLGGNVSFKAKPYLDRAGSALNVHVNLVNSNNGNLFYINEQKYSDYLIHSIGGLCAMMKKHMLFFAPNDDSYLRFQY
PDIHTPTTISWGVNNRTAAIRIPYFGNDFKRCRLEHRVPGADCDLEKVLTAIIEGMVFGIENKIAPPNRVYGIASDPQYE
MERLI

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081870, Length=185, Percent_Identity=34.5945945945946, Blast_Score=104, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 27955; Mature: 27955

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILSNLSCYAVFGIELEFYIEGIEKEYLFLNSIKNKIASLEFSCEKENSTHQYEIKSGC
CEEEECCCEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCC
YTNSYNLIKHFELAKQSLTETAQKLGGNVSFKAKPYLDRAGSALNVHVNLVNSNNGNLFY
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCEEEEEEEEEECCCCCEEE
INEQKYSDYLIHSIGGLCAMMKKHMLFFAPNDDSYLRFQYPDIHTPTTISWGVNNRTAAI
EECCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEEE
RIPYFGNDFKRCRLEHRVPGADCDLEKVLTAIIEGMVFGIENKIAPPNRVYGIASDPQYE
EECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHH
MERLI
HHHCC
>Mature Secondary Structure
MIILSNLSCYAVFGIELEFYIEGIEKEYLFLNSIKNKIASLEFSCEKENSTHQYEIKSGC
CEEEECCCEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCC
YTNSYNLIKHFELAKQSLTETAQKLGGNVSFKAKPYLDRAGSALNVHVNLVNSNNGNLFY
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCEEEEEEEEEECCCCCEEE
INEQKYSDYLIHSIGGLCAMMKKHMLFFAPNDDSYLRFQYPDIHTPTTISWGVNNRTAAI
EECCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEEE
RIPYFGNDFKRCRLEHRVPGADCDLEKVLTAIIEGMVFGIENKIAPPNRVYGIASDPQYE
EECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHH
MERLI
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]