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The map label for this gene is recF [H]

Identifier: 225630989

GI number: 225630989

Start: 1401283

End: 1402380

Strand: Direct

Name: recF [H]

Synonym: WRi_013130

Alternate gene names: 225630989

Gene position: 1401283-1402380 (Clockwise)

Preceding gene: 225630988

Following gene: 225630993

Centisome position: 96.92

GC content: 33.42

Gene sequence:

>1098_bases
ATGGCTACCCATTGCTACATAAAAAAGCTGAAATTACATAATTTTCGTAGCCACTCAAACTTTGAACTGGATTCAGATGA
TAGCTCAGTTGTAATAACTGGCAAAAACGGTATTGGTAAAACTAATATACTTGAAGCAATCTCGTTGCTTGCTAAAAGCA
ATGGAATGAAAAAAGCAAAAGCCAGTGAGATACAAAATAGATTCAGTAATGAAGATTGGGTAGTGCACTATGATTTTTTT
AATGGAATGGATTTTAATTCAATTGGTATCGCAAAGAGTTTTGATAAGAAACTAATACAAATTGATGGAAAAACGCAATC
GAGTTATTCATCTTTGTATAAGATATCCAATGTAATATGGCTGATTCCACAAATGGACTATGTTCTTCTTAATTCTCCAA
GTGACAGATTAAAATTTTTAGACCGTATAGTCTCACTGTTTGAGGAAAATTACACTTGCTGCTACATGAAACACAGAAAA
GCTAAACGTGAGCGAAGTAAACTGTTGAGAGAGAACACCTTGGACAAAAATTGGCTCTCTAGTCTTGAAAACATAATGGC
CGTTAATGCAGTTAGTATTTTACGTATGCGATCGTCTGTTTTAAAGACACTACAAGACACAATTGATAACCATTCCGGTG
AGCTTTTTCCGAAGGCAAGTTTGAAATTCAGCAGCCAGCTAACTTTGGACGATACTGCAGAATATTTTCAGAATCGTCTA
AAGGAAAACAGAGAAAAAGATTCATTGACTGGTAGAGTAACTTTTGGTGTGCATAACGATAATTTTCGGGTTTTTTGTCA
AAAAAGAAACGTACCAATAAATCTGTGCTCCACTGGAGAACAAAAGTTATTACTGCTTTCTATTATTTTATCCAGTGTAA
AAGCAAGGTGTATTCATTACAATAAGGCACCACTTCTTCTACTTGATGATATAATGTCTCATCTGGATAAACATTACAGA
AAGGCGTTAATAGAGGAAGTGTTAAGTATTCAATGTCAAACTTGGATAACTGATGTAAATCAAGATAATTTCAATAGCTA
TCTTTATTCTTTCAAGTTTTTTGAATTATCAAATGAATTAAATGCAAAACTTCCTTGA

Upstream 100 bases:

>100_bases
AAAGGTAAAATAGTGAAGATCTGGAAAAATGTAAAAGTTAGTGGACATGTTGATGAGGTTCTGGAAGAAGTAAAGAGAAT
ATAAACTAAAGAATAGGTTA

Downstream 100 bases:

>100_bases
CAAACCTCGCCACGCCCCCTTATCATGATACTGAAGGTATTCAGTTATCTTCATCTGTGCAGATTAAACAGCAAGAAAAC
AACGTAGTTGGCGTCTTATT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MATHCYIKKLKLHNFRSHSNFELDSDDSSVVITGKNGIGKTNILEAISLLAKSNGMKKAKASEIQNRFSNEDWVVHYDFF
NGMDFNSIGIAKSFDKKLIQIDGKTQSSYSSLYKISNVIWLIPQMDYVLLNSPSDRLKFLDRIVSLFEENYTCCYMKHRK
AKRERSKLLRENTLDKNWLSSLENIMAVNAVSILRMRSSVLKTLQDTIDNHSGELFPKASLKFSSQLTLDDTAEYFQNRL
KENREKDSLTGRVTFGVHNDNFRVFCQKRNVPINLCSTGEQKLLLLSIILSSVKARCIHYNKAPLLLLDDIMSHLDKHYR
KALIEEVLSIQCQTWITDVNQDNFNSYLYSFKFFELSNELNAKLP

Sequences:

>Translated_365_residues
MATHCYIKKLKLHNFRSHSNFELDSDDSSVVITGKNGIGKTNILEAISLLAKSNGMKKAKASEIQNRFSNEDWVVHYDFF
NGMDFNSIGIAKSFDKKLIQIDGKTQSSYSSLYKISNVIWLIPQMDYVLLNSPSDRLKFLDRIVSLFEENYTCCYMKHRK
AKRERSKLLRENTLDKNWLSSLENIMAVNAVSILRMRSSVLKTLQDTIDNHSGELFPKASLKFSSQLTLDDTAEYFQNRL
KENREKDSLTGRVTFGVHNDNFRVFCQKRNVPINLCSTGEQKLLLLSIILSSVKARCIHYNKAPLLLLDDIMSHLDKHYR
KALIEEVLSIQCQTWITDVNQDNFNSYLYSFKFFELSNELNAKLP
>Mature_364_residues
ATHCYIKKLKLHNFRSHSNFELDSDDSSVVITGKNGIGKTNILEAISLLAKSNGMKKAKASEIQNRFSNEDWVVHYDFFN
GMDFNSIGIAKSFDKKLIQIDGKTQSSYSSLYKISNVIWLIPQMDYVLLNSPSDRLKFLDRIVSLFEENYTCCYMKHRKA
KRERSKLLRENTLDKNWLSSLENIMAVNAVSILRMRSSVLKTLQDTIDNHSGELFPKASLKFSSQLTLDDTAEYFQNRLK
ENREKDSLTGRVTFGVHNDNFRVFCQKRNVPINLCSTGEQKLLLLSIILSSVKARCIHYNKAPLLLLDDIMSHLDKHYRK
ALIEEVLSIQCQTWITDVNQDNFNSYLYSFKFFELSNELNAKLP

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=346, Percent_Identity=23.4104046242775, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 42159; Mature: 42028

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATHCYIKKLKLHNFRSHSNFELDSDDSSVVITGKNGIGKTNILEAISLLAKSNGMKKAK
CCCHHHHHHHHHHCCCCCCCCEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHH
ASEIQNRFSNEDWVVHYDFFNGMDFNSIGIAKSFDKKLIQIDGKTQSSYSSLYKISNVIW
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHCCCEEEECCCCHHHHHHHHHHHCEEE
LIPQMDYVLLNSPSDRLKFLDRIVSLFEENYTCCYMKHRKAKRERSKLLRENTLDKNWLS
EECCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHH
SLENIMAVNAVSILRMRSSVLKTLQDTIDNHSGELFPKASLKFSSQLTLDDTAEYFQNRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
KENREKDSLTGRVTFGVHNDNFRVFCQKRNVPINLCSTGEQKLLLLSIILSSVKARCIHY
HHCCCCCCCEEEEEEEEECCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHEEC
NKAPLLLLDDIMSHLDKHYRKALIEEVLSIQCQTWITDVNQDNFNSYLYSFKFFELSNEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEEEEEECCCC
NAKLP
CCCCC
>Mature Secondary Structure 
ATHCYIKKLKLHNFRSHSNFELDSDDSSVVITGKNGIGKTNILEAISLLAKSNGMKKAK
CCHHHHHHHHHHCCCCCCCCEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHH
ASEIQNRFSNEDWVVHYDFFNGMDFNSIGIAKSFDKKLIQIDGKTQSSYSSLYKISNVIW
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHCCCEEEECCCCHHHHHHHHHHHCEEE
LIPQMDYVLLNSPSDRLKFLDRIVSLFEENYTCCYMKHRKAKRERSKLLRENTLDKNWLS
EECCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHH
SLENIMAVNAVSILRMRSSVLKTLQDTIDNHSGELFPKASLKFSSQLTLDDTAEYFQNRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
KENREKDSLTGRVTFGVHNDNFRVFCQKRNVPINLCSTGEQKLLLLSIILSSVKARCIHY
HHCCCCCCCEEEEEEEEECCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHEEC
NKAPLLLLDDIMSHLDKHYRKALIEEVLSIQCQTWITDVNQDNFNSYLYSFKFFELSNEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEEEEEECCCC
NAKLP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]