Click here to switch to the map view.

The map label for this gene is pleC [H]

Identifier: 225630987

GI number: 225630987

Start: 1399182

End: 1400609

Strand: Direct

Name: pleC [H]

Synonym: WRi_013110

Alternate gene names: 225630987

Gene position: 1399182-1400609 (Clockwise)

Preceding gene: 225630982

Following gene: 225630988

Centisome position: 96.77

GC content: 28.92

Gene sequence:

>1428_bases
ATGTATAAGATAGGTAAATTAATGTTTTCTGCACTGGGAAAAAACAAATACACAAAAGTTATCGCATTATTCTCATCAAT
TTTTATGATTTTATTCAGTGTGATATATTGTAATTATTTACTAAGGAGTAATTTCCTTTCTTCTTACCGTGATTCAAGTG
TAAACTTAAAAAGTGTATTAGAAAATGGTATAATAAAAAAATACCATTATTTACTAATAGAAAAGCATCACATTAAATAC
AAAAATTTTGACTACATAAACCAACTAGTAAAACTACGTGCTGAATTATTACAATCAGTGAGCAAAGTAAAGGATTTCAG
CTTAATACTGTATGATCAAAACGGCAGAATAATATTCAGTAATTTTAACACTCAAGATTATGATTATGAACAGTTACTTA
CAAACGATGAAATTGATAAATTATTAAGTGACCAAGAAATACTTTATACTACAGGAAATACATTAACTTCTATTTTCCCT
ATATTCCATGAAAATAGCGCTAAGCCGTCGTTTTTTTTGAAGATTCTTCGAAGCTACAATAATTCTTATGTTATAGCATA
TGGCCTATTTTTAATATTTTTAGGCTTATTATTAATAATTTTGATATTAATAATGTTCTATTTGCACTTTTCTAATACCA
AAATGCTGGCCAAGCAACATAAAACTAACATTGAATTACAGCAGATAAAAGAGGCATTAGAGCAAGAAAATGCAAACAAG
CTAAAGTTTTTTGCAAGCGTTACACATGAATTACGCACACCTCTTAATGCTATTATTGGATTTGCAGAGTTGATCAAAAA
TGAAACTTTAGGTTCAATGGATGACTCTCAATACAAGGAGTATGCAGACGATATATATAATGCAGGGACGCATTTACTTG
CTTTAATTAACGATGTGCTCGATTTTTCAAAAGCTGAATCAAGTAGTTTAACAGTAGAAAAAGTGAAGTTTAACCTGAAC
AAAATAATAGATTCATGTGTAAATATGCTATTGCCTAAATTAAAAGAAGCCGGGATTAATTTAAAAAAAGAGATGACTAA
TAAACAATTATTAGTCATTGCAGATCCCAAGAGAATGAAACAAGTTATAATAAATTTATTATCAAACTCAATCAAGTTTA
CTCCTAAAGATGGTTTAATAAGAATGGTGATAAAAGAAAATATAGAAAAAAACTTATTAACCATTGAGTTTCATGACAAT
GGAATAGGAATAATGCAGCAGGATATATATAAAGTTATGTCTGTTTTTGGCCAAGCAGATTCGGGATATAGAAACGAAGG
TACGGGCATCGGATTACCGTTAAGCAAGAAATTAGTAGAGTTAATGGGTGGAACTTTTGACATTAAAAGTGAAGCAAAAC
TTGGTACTACGATAACATTGAACTTCTTTTATGAAGAACAAACATGTGAAGATTCGATTGATTTTTAA

Upstream 100 bases:

>100_bases
GAGAATAAACTGGCAAAATAGGAGAAATTGTCATAAAATATTATATGCTAAATTTTATTATTAATAACATAAAAGCCGTT
GTTTCGCAATGAGTAAGGAT

Downstream 100 bases:

>100_bases
TAGGATCTATTTGATATGGCCTTACATCAGACGAAAACCACACTTTATCAAAAGCGGCAAGCCGAGAAGGTAGGCAGAAA
TTCATCAATAAGCCCCCGTC

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MYKIGKLMFSALGKNKYTKVIALFSSIFMILFSVIYCNYLLRSNFLSSYRDSSVNLKSVLENGIIKKYHYLLIEKHHIKY
KNFDYINQLVKLRAELLQSVSKVKDFSLILYDQNGRIIFSNFNTQDYDYEQLLTNDEIDKLLSDQEILYTTGNTLTSIFP
IFHENSAKPSFFLKILRSYNNSYVIAYGLFLIFLGLLLIILILIMFYLHFSNTKMLAKQHKTNIELQQIKEALEQENANK
LKFFASVTHELRTPLNAIIGFAELIKNETLGSMDDSQYKEYADDIYNAGTHLLALINDVLDFSKAESSSLTVEKVKFNLN
KIIDSCVNMLLPKLKEAGINLKKEMTNKQLLVIADPKRMKQVIINLLSNSIKFTPKDGLIRMVIKENIEKNLLTIEFHDN
GIGIMQQDIYKVMSVFGQADSGYRNEGTGIGLPLSKKLVELMGGTFDIKSEAKLGTTITLNFFYEEQTCEDSIDF

Sequences:

>Translated_475_residues
MYKIGKLMFSALGKNKYTKVIALFSSIFMILFSVIYCNYLLRSNFLSSYRDSSVNLKSVLENGIIKKYHYLLIEKHHIKY
KNFDYINQLVKLRAELLQSVSKVKDFSLILYDQNGRIIFSNFNTQDYDYEQLLTNDEIDKLLSDQEILYTTGNTLTSIFP
IFHENSAKPSFFLKILRSYNNSYVIAYGLFLIFLGLLLIILILIMFYLHFSNTKMLAKQHKTNIELQQIKEALEQENANK
LKFFASVTHELRTPLNAIIGFAELIKNETLGSMDDSQYKEYADDIYNAGTHLLALINDVLDFSKAESSSLTVEKVKFNLN
KIIDSCVNMLLPKLKEAGINLKKEMTNKQLLVIADPKRMKQVIINLLSNSIKFTPKDGLIRMVIKENIEKNLLTIEFHDN
GIGIMQQDIYKVMSVFGQADSGYRNEGTGIGLPLSKKLVELMGGTFDIKSEAKLGTTITLNFFYEEQTCEDSIDF
>Mature_475_residues
MYKIGKLMFSALGKNKYTKVIALFSSIFMILFSVIYCNYLLRSNFLSSYRDSSVNLKSVLENGIIKKYHYLLIEKHHIKY
KNFDYINQLVKLRAELLQSVSKVKDFSLILYDQNGRIIFSNFNTQDYDYEQLLTNDEIDKLLSDQEILYTTGNTLTSIFP
IFHENSAKPSFFLKILRSYNNSYVIAYGLFLIFLGLLLIILILIMFYLHFSNTKMLAKQHKTNIELQQIKEALEQENANK
LKFFASVTHELRTPLNAIIGFAELIKNETLGSMDDSQYKEYADDIYNAGTHLLALINDVLDFSKAESSSLTVEKVKFNLN
KIIDSCVNMLLPKLKEAGINLKKEMTNKQLLVIADPKRMKQVIINLLSNSIKFTPKDGLIRMVIKENIEKNLLTIEFHDN
GIGIMQQDIYKVMSVFGQADSGYRNEGTGIGLPLSKKLVELMGGTFDIKSEAKLGTTITLNFFYEEQTCEDSIDF

Specific function: Member of the two-component regulatory system involved in the regulation of polar organelle development. PleC functions as a membrane-associated protein kinase that transfers phosphate to the response regulator pleD, leading to its activation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=244, Percent_Identity=35.655737704918, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI145693157, Length=239, Percent_Identity=35.1464435146443, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI87081816, Length=245, Percent_Identity=30.2040816326531, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI48994928, Length=242, Percent_Identity=29.3388429752066, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1788713, Length=230, Percent_Identity=30, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786912, Length=229, Percent_Identity=26.2008733624454, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788549, Length=222, Percent_Identity=26.1261261261261, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786783, Length=242, Percent_Identity=24.3801652892562, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790861, Length=292, Percent_Identity=22.6027397260274, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1790436, Length=216, Percent_Identity=25.462962962963, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788393, Length=252, Percent_Identity=24.2063492063492, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54541; Mature: 54541

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKIGKLMFSALGKNKYTKVIALFSSIFMILFSVIYCNYLLRSNFLSSYRDSSVNLKSVL
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ENGIIKKYHYLLIEKHHIKYKNFDYINQLVKLRAELLQSVSKVKDFSLILYDQNGRIIFS
HCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEE
NFNTQDYDYEQLLTNDEIDKLLSDQEILYTTGNTLTSIFPIFHENSAKPSFFLKILRSYN
CCCCCCCCHHHHHCCHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
NSYVIAYGLFLIFLGLLLIILILIMFYLHFSNTKMLAKQHKTNIELQQIKEALEQENANK
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCHH
LKFFASVTHELRTPLNAIIGFAELIKNETLGSMDDSQYKEYADDIYNAGTHLLALINDVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHH
DFSKAESSSLTVEKVKFNLNKIIDSCVNMLLPKLKEAGINLKKEMTNKQLLVIADPKRMK
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECHHHHH
QVIINLLSNSIKFTPKDGLIRMVIKENIEKNLLTIEFHDNGIGIMQQDIYKVMSVFGQAD
HHHHHHHHCCCEECCCCCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCC
SGYRNEGTGIGLPLSKKLVELMGGTFDIKSEAKLGTTITLNFFYEEQTCEDSIDF
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MYKIGKLMFSALGKNKYTKVIALFSSIFMILFSVIYCNYLLRSNFLSSYRDSSVNLKSVL
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ENGIIKKYHYLLIEKHHIKYKNFDYINQLVKLRAELLQSVSKVKDFSLILYDQNGRIIFS
HCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEE
NFNTQDYDYEQLLTNDEIDKLLSDQEILYTTGNTLTSIFPIFHENSAKPSFFLKILRSYN
CCCCCCCCHHHHHCCHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
NSYVIAYGLFLIFLGLLLIILILIMFYLHFSNTKMLAKQHKTNIELQQIKEALEQENANK
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCHH
LKFFASVTHELRTPLNAIIGFAELIKNETLGSMDDSQYKEYADDIYNAGTHLLALINDVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHH
DFSKAESSSLTVEKVKFNLNKIIDSCVNMLLPKLKEAGINLKKEMTNKQLLVIADPKRMK
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECHHHHH
QVIINLLSNSIKFTPKDGLIRMVIKENIEKNLLTIEFHDNGIGIMQQDIYKVMSVFGQAD
HHHHHHHHCCCEECCCCCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCC
SGYRNEGTGIGLPLSKKLVELMGGTFDIKSEAKLGTTITLNFFYEEQTCEDSIDF
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8421698; 11259647 [H]