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The map label for this gene is ruvB [H]

Identifier: 225630964

GI number: 225630964

Start: 1374266

End: 1375240

Strand: Reverse

Name: ruvB [H]

Synonym: WRi_012860

Alternate gene names: 225630964

Gene position: 1375240-1374266 (Counterclockwise)

Preceding gene: 225630965

Following gene: 225630963

Centisome position: 95.11

GC content: 38.87

Gene sequence:

>975_bases
ATGAAATCAATATCGTGCGGTAAAGAATATACTGAAGATGTGCGCAATATTAACATCAGGCCTGAGCAACTTGATGATTT
TGTCGGGCAAAAAGACTTAATACAAAATTTAAAAGTGTTTATAAACGCTGCACAGACGAGAACTGAAGCTTTAGATCACG
TCTTATTGTACGGTCCTCCAGGGCTTGGCAAAACAACTTTAGCACAAATTGTCTCTAAAGAGTTAAGAGTCAGCTTCCGC
GCAACTTCTGGTCCTTTACTTAGTAAAGCTGGAGACTTGGCCGCAGTGCTTACCACTTTAAATGCAAAAGATGTTCTATT
TATCGACGAAATTCATAGATTAAATCGCAGCATCGAAGAAGTTTTATATACTGCTATGGAAGATTTTTGCTTGGACATAT
TAGTAGGTGAAGGTCCATCTACTCGCACTTTAAGGATAGATCTACCACCATTTACGCTAATTGGAGCAACAACGCGGCTT
GGATTACTTTCTGCACCACTGAGGGATCGCTTTGGCATTCCTTTGCATCTTGAGTTTTATTCCTTTGAGGAGCTGGTTAA
TATTATAAAAAGAGGCGCAAGAGTTCTTTCTGCTGAAATTGAAGAGGATGCAGCACGGGAAATTGCCTGCCGTGCGCGCG
GCACTCCAAGAATTGCTTTGAGGTTACTCAGAAGAATAAGGGATTTTGTTGAAGTGAAAGATGATAAAAAAATTACTTAT
GAAGTCGCTGATTCTGTATTACTAAAATTGGGTGTAGATAAAATGGGACTCAATAAACTTGATATGAATTATCTAAGATT
TTTATTCAACACTTCAGGCCCTGTTGGAATTGACACCATATCTATTGCGCTATCCGAAGATGTTGGCAATATTGAAGAAA
CAGTAGAACCTTATTTAATCAAAATTAGTTTTGTAAAGCGCACACCAAGAGGGCGCGTTCTAACCGATCAAGCAAAGGAG
TATTTGAGCCTTTAA

Upstream 100 bases:

>100_bases
GATATGAAAAAATGAAAGCTTATGATACGATAAAAAAATACAGGCCAAATCTGGACACTAAGGATATTATTCGCATGGCG
CTTAAGGAGCTCTCAACATT

Downstream 100 bases:

>100_bases
TATTAGAGAGCTTTTCTACCATAATTCAATTATATATATTGATTGGCATAATATCATATACTGGCATCTTTTTGTATGAA
GGCATATGTGTTAAATAGCA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKSISCGKEYTEDVRNINIRPEQLDDFVGQKDLIQNLKVFINAAQTRTEALDHVLLYGPPGLGKTTLAQIVSKELRVSFR
ATSGPLLSKAGDLAAVLTTLNAKDVLFIDEIHRLNRSIEEVLYTAMEDFCLDILVGEGPSTRTLRIDLPPFTLIGATTRL
GLLSAPLRDRFGIPLHLEFYSFEELVNIIKRGARVLSAEIEEDAAREIACRARGTPRIALRLLRRIRDFVEVKDDKKITY
EVADSVLLKLGVDKMGLNKLDMNYLRFLFNTSGPVGIDTISIALSEDVGNIEETVEPYLIKISFVKRTPRGRVLTDQAKE
YLSL

Sequences:

>Translated_324_residues
MKSISCGKEYTEDVRNINIRPEQLDDFVGQKDLIQNLKVFINAAQTRTEALDHVLLYGPPGLGKTTLAQIVSKELRVSFR
ATSGPLLSKAGDLAAVLTTLNAKDVLFIDEIHRLNRSIEEVLYTAMEDFCLDILVGEGPSTRTLRIDLPPFTLIGATTRL
GLLSAPLRDRFGIPLHLEFYSFEELVNIIKRGARVLSAEIEEDAAREIACRARGTPRIALRLLRRIRDFVEVKDDKKITY
EVADSVLLKLGVDKMGLNKLDMNYLRFLFNTSGPVGIDTISIALSEDVGNIEETVEPYLIKISFVKRTPRGRVLTDQAKE
YLSL
>Mature_324_residues
MKSISCGKEYTEDVRNINIRPEQLDDFVGQKDLIQNLKVFINAAQTRTEALDHVLLYGPPGLGKTTLAQIVSKELRVSFR
ATSGPLLSKAGDLAAVLTTLNAKDVLFIDEIHRLNRSIEEVLYTAMEDFCLDILVGEGPSTRTLRIDLPPFTLIGATTRL
GLLSAPLRDRFGIPLHLEFYSFEELVNIIKRGARVLSAEIEEDAAREIACRARGTPRIALRLLRRIRDFVEVKDDKKITY
EVADSVLLKLGVDKMGLNKLDMNYLRFLFNTSGPVGIDTISIALSEDVGNIEETVEPYLIKISFVKRTPRGRVLTDQAKE
YLSL

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=323, Percent_Identity=53.5603715170279, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI1787119, Length=233, Percent_Identity=27.0386266094421, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 36292; Mature: 36292

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSISCGKEYTEDVRNINIRPEQLDDFVGQKDLIQNLKVFINAAQTRTEALDHVLLYGPP
CCCCCCCHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCC
GLGKTTLAQIVSKELRVSFRATSGPLLSKAGDLAAVLTTLNAKDVLFIDEIHRLNRSIEE
CCCHHHHHHHHHHHHHEEEECCCCCHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
VLYTAMEDFCLDILVGEGPSTRTLRIDLPPFTLIGATTRLGLLSAPLRDRFGIPLHLEFY
HHHHHHHHHHHHHEECCCCCCEEEEEECCCEEEHHHHHHHHHHCCCHHHHCCCCEEEEHH
SFEELVNIIKRGARVLSAEIEEDAAREIACRARGTPRIALRLLRRIRDFVEVKDDKKITY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEHH
EVADSVLLKLGVDKMGLNKLDMNYLRFLFNTSGPVGIDTISIALSEDVGNIEETVEPYLI
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHCCCEEE
KISFVKRTPRGRVLTDQAKEYLSL
EEEEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure
MKSISCGKEYTEDVRNINIRPEQLDDFVGQKDLIQNLKVFINAAQTRTEALDHVLLYGPP
CCCCCCCHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCC
GLGKTTLAQIVSKELRVSFRATSGPLLSKAGDLAAVLTTLNAKDVLFIDEIHRLNRSIEE
CCCHHHHHHHHHHHHHEEEECCCCCHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
VLYTAMEDFCLDILVGEGPSTRTLRIDLPPFTLIGATTRLGLLSAPLRDRFGIPLHLEFY
HHHHHHHHHHHHHEECCCCCCEEEEEECCCEEEHHHHHHHHHHCCCHHHHCCCCEEEEHH
SFEELVNIIKRGARVLSAEIEEDAAREIACRARGTPRIALRLLRRIRDFVEVKDDKKITY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEHH
EVADSVLLKLGVDKMGLNKLDMNYLRFLFNTSGPVGIDTISIALSEDVGNIEETVEPYLI
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHCCCEEE
KISFVKRTPRGRVLTDQAKEYLSL
EEEEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA