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The map label for this gene is purF [H]

Identifier: 225630962

GI number: 225630962

Start: 1371951

End: 1373336

Strand: Direct

Name: purF [H]

Synonym: WRi_012840

Alternate gene names: 225630962

Gene position: 1371951-1373336 (Clockwise)

Preceding gene: 225630961

Following gene: 225630969

Centisome position: 94.89

GC content: 35.86

Gene sequence:

>1386_bases
ATGCTAGATGAAATGCGTGAGGAGTGTGGGGTATTTGGCATAAGTTGCAACCAAAGTGCTGCTTTTAACTCTATACTTGC
TCTCCACGCTTTGCAGCATAGAGGTCAAGAGTCTTTTGGCGTAGTAACCAGTAACAACGATAAGCTGCACTCTTATCACT
TTCAAGGTCAAGTGAGCAGCATATTTGATGATATAGATGAAATAAAGAAATCCTTACCTGGAGATTGTGCAATAGGTCAT
GTTCGATACTCAACAAGTGGTAGTAAATTTGGAGTGCAGCCCATGTTTGGCAAAAGTGGCAAATTTGGGGATTTCGCCAT
AGCACATAATGGTAATTTAATTAATATTTCTCCAATACGCGAACAATTAATTAAACAAGAGTGCGTTTTTCAGTCAGATA
TTGATACAGAAGTAGTAGTGCATCTGACAGCAAGCGGTGAAAAAGATAGCTTTTTAGAGAGTTTTATATATGCATTAAAG
CAAATACAAGGTGCTTATTCTTTTGTGGCAATCAATCAAGAAGTAGTTATTGGAGTACGTGATCCATCTGGAATCAGGCC
TCTTGTTTTAGGGAAGTTAAACGGTTCTTATGTTCTTGCATCTGAAACTTGTGCTCTTGATATAGTAAATGCAGAGTTTG
TTAGAGAAATAGAACCAGGTGAGTTAGTCACCATTGATCGAAATGGTAATCTAGCTTCAGCATTTCCTTTCCCACAGCAA
AAATCAAGTTTTTGTATTTTTGAATACGTTTATTTTTCAAGACCAGACAGCATAATGGAGAATAGGTCCATATATGATAT
AAGAAAAGAAATAGGAAGAATACTTGCAGAAGAGAGCCCACCAAAAAACAATATGGATATGGTTGTACCAATACCTGATT
CTGGAATACCTGCAGCTATTGGATATGCAAAGCATTCAGGATTACCTATGGAACTGGGAATAATTCGTAATCATTACATA
GGAAGAACTTTTATACAACCAACCGCTGAAGTACGTAAAGTTAGAATAAAATTAAAATTCAATGCTAATAAGCACACTTT
GAAAGGTAAAAACATAATTTTAATAGATGATAGTATAGTGCGTGGTAGTACGTTAACAAATATAATAGTTATGTTAAAGG
ATGCAGGAGTAAAAGAGATTCACCTCAAAATTTCAAGCCCACCAATTAAGCATTCTTGTTTTTATGGAATAGATACGCCA
GAGTGCAAAGATTTAATTGCTGCAAATAAATCTGTAAAGGAAATTAAGGAAATTATAGGAGTAGATAGCCTAGCCTTCTT
GAGTATTGATGGACTATATCAGGCTGTTAAAGGAGAGGTGCGTAACAATGCTATACCACAATATTGTGATGCTTGTTTCA
CTGGTGATTATCCGATTGGCAAATAG

Upstream 100 bases:

>100_bases
ATATGTATAGGGCTATTGCTAGCAATAAAAGCAGATGAACAACAAAATACTTGTATTTTACCTATGCTAAAGTAAAATTT
AACTTTACTTAATAGTTTTT

Downstream 100 bases:

>100_bases
TCTACGCAAGGTCTTAAGTTTAGAAAATCTTAAACCGCCAAAATTTCTCTTGGCCTTTACTAATTCCGATTCTCGGGGTG
CAAATATAATCTGGTTTGAG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLDEMREECGVFGISCNQSAAFNSILALHALQHRGQESFGVVTSNNDKLHSYHFQGQVSSIFDDIDEIKKSLPGDCAIGH
VRYSTSGSKFGVQPMFGKSGKFGDFAIAHNGNLINISPIREQLIKQECVFQSDIDTEVVVHLTASGEKDSFLESFIYALK
QIQGAYSFVAINQEVVIGVRDPSGIRPLVLGKLNGSYVLASETCALDIVNAEFVREIEPGELVTIDRNGNLASAFPFPQQ
KSSFCIFEYVYFSRPDSIMENRSIYDIRKEIGRILAEESPPKNNMDMVVPIPDSGIPAAIGYAKHSGLPMELGIIRNHYI
GRTFIQPTAEVRKVRIKLKFNANKHTLKGKNIILIDDSIVRGSTLTNIIVMLKDAGVKEIHLKISSPPIKHSCFYGIDTP
ECKDLIAANKSVKEIKEIIGVDSLAFLSIDGLYQAVKGEVRNNAIPQYCDACFTGDYPIGK

Sequences:

>Translated_461_residues
MLDEMREECGVFGISCNQSAAFNSILALHALQHRGQESFGVVTSNNDKLHSYHFQGQVSSIFDDIDEIKKSLPGDCAIGH
VRYSTSGSKFGVQPMFGKSGKFGDFAIAHNGNLINISPIREQLIKQECVFQSDIDTEVVVHLTASGEKDSFLESFIYALK
QIQGAYSFVAINQEVVIGVRDPSGIRPLVLGKLNGSYVLASETCALDIVNAEFVREIEPGELVTIDRNGNLASAFPFPQQ
KSSFCIFEYVYFSRPDSIMENRSIYDIRKEIGRILAEESPPKNNMDMVVPIPDSGIPAAIGYAKHSGLPMELGIIRNHYI
GRTFIQPTAEVRKVRIKLKFNANKHTLKGKNIILIDDSIVRGSTLTNIIVMLKDAGVKEIHLKISSPPIKHSCFYGIDTP
ECKDLIAANKSVKEIKEIIGVDSLAFLSIDGLYQAVKGEVRNNAIPQYCDACFTGDYPIGK
>Mature_461_residues
MLDEMREECGVFGISCNQSAAFNSILALHALQHRGQESFGVVTSNNDKLHSYHFQGQVSSIFDDIDEIKKSLPGDCAIGH
VRYSTSGSKFGVQPMFGKSGKFGDFAIAHNGNLINISPIREQLIKQECVFQSDIDTEVVVHLTASGEKDSFLESFIYALK
QIQGAYSFVAINQEVVIGVRDPSGIRPLVLGKLNGSYVLASETCALDIVNAEFVREIEPGELVTIDRNGNLASAFPFPQQ
KSSFCIFEYVYFSRPDSIMENRSIYDIRKEIGRILAEESPPKNNMDMVVPIPDSGIPAAIGYAKHSGLPMELGIIRNHYI
GRTFIQPTAEVRKVRIKLKFNANKHTLKGKNIILIDDSIVRGSTLTNIIVMLKDAGVKEIHLKISSPPIKHSCFYGIDTP
ECKDLIAANKSVKEIKEIIGVDSLAFLSIDGLYQAVKGEVRNNAIPQYCDACFTGDYPIGK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=518, Percent_Identity=34.9420849420849, Blast_Score=307, Evalue=1e-83,
Organism=Escherichia coli, GI1788651, Length=471, Percent_Identity=36.3057324840764, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1790167, Length=228, Percent_Identity=28.0701754385965, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=481, Percent_Identity=37.8378378378378, Blast_Score=304, Evalue=6e-83,
Organism=Caenorhabditis elegans, GI17539970, Length=183, Percent_Identity=27.3224043715847, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17532897, Length=183, Percent_Identity=27.3224043715847, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17532899, Length=183, Percent_Identity=27.3224043715847, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323958, Length=482, Percent_Identity=31.3278008298755, Blast_Score=226, Evalue=8e-60,
Organism=Drosophila melanogaster, GI24659598, Length=493, Percent_Identity=38.5395537525355, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24659604, Length=466, Percent_Identity=38.8412017167382, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI28573187, Length=498, Percent_Identity=36.5461847389558, Blast_Score=311, Evalue=5e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 50846; Mature: 50846

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDEMREECGVFGISCNQSAAFNSILALHALQHRGQESFGVVTSNNDKLHSYHFQGQVSS
CCHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCHHH
IFDDIDEIKKSLPGDCAIGHVRYSTSGSKFGVQPMFGKSGKFGDFAIAHNGNLINISPIR
HHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEHHHH
EQLIKQECVFQSDIDTEVVVHLTASGEKDSFLESFIYALKQIQGAYSFVAINQEVVIGVR
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEC
DPSGIRPLVLGKLNGSYVLASETCALDIVNAEFVREIEPGELVTIDRNGNLASAFPFPQQ
CCCCCCCEEEEECCCCEEEEECCEEEEEECHHHHHCCCCCCEEEEECCCCEEECCCCCCC
KSSFCIFEYVYFSRPDSIMENRSIYDIRKEIGRILAEESPPKNNMDMVVPIPDSGIPAAI
CCCEEEEEEEECCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHE
GYAKHSGLPMELGIIRNHYIGRTFIQPTAEVRKVRIKLKFNANKHTLKGKNIILIDDSIV
EHHHCCCCCEEEHHHHCCCCCCHHCCCCCCEEEEEEEEEECCCCEEECCCEEEEECCCCC
RGSTLTNIIVMLKDAGVKEIHLKISSPPIKHSCFYGIDTPECKDLIAANKSVKEIKEIIG
CCCHHHEEEEEEECCCCEEEEEEECCCCCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHC
VDSLAFLSIDGLYQAVKGEVRNNAIPQYCDACFTGDYPIGK
CCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLDEMREECGVFGISCNQSAAFNSILALHALQHRGQESFGVVTSNNDKLHSYHFQGQVSS
CCHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCHHH
IFDDIDEIKKSLPGDCAIGHVRYSTSGSKFGVQPMFGKSGKFGDFAIAHNGNLINISPIR
HHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEHHHH
EQLIKQECVFQSDIDTEVVVHLTASGEKDSFLESFIYALKQIQGAYSFVAINQEVVIGVR
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEC
DPSGIRPLVLGKLNGSYVLASETCALDIVNAEFVREIEPGELVTIDRNGNLASAFPFPQQ
CCCCCCCEEEEECCCCEEEEECCEEEEEECHHHHHCCCCCCEEEEECCCCEEECCCCCCC
KSSFCIFEYVYFSRPDSIMENRSIYDIRKEIGRILAEESPPKNNMDMVVPIPDSGIPAAI
CCCEEEEEEEECCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHE
GYAKHSGLPMELGIIRNHYIGRTFIQPTAEVRKVRIKLKFNANKHTLKGKNIILIDDSIV
EHHHCCCCCEEEHHHHCCCCCCHHCCCCCCEEEEEEEEEECCCCEEECCCEEEEECCCCC
RGSTLTNIIVMLKDAGVKEIHLKISSPPIKHSCFYGIDTPECKDLIAANKSVKEIKEIIG
CCCHHHEEEEEEECCCCEEEEEEECCCCCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHC
VDSLAFLSIDGLYQAVKGEVRNNAIPQYCDACFTGDYPIGK
CCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]