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The map label for this gene is ispH [H]

Identifier: 225630927

GI number: 225630927

Start: 1336345

End: 1337274

Strand: Direct

Name: ispH [H]

Synonym: WRi_012440

Alternate gene names: 225630927

Gene position: 1336345-1337274 (Clockwise)

Preceding gene: 225630925

Following gene: 225630933

Centisome position: 92.42

GC content: 36.02

Gene sequence:

>930_bases
ATGGAAATCATCTTAGCTGAACCACGAGGTTTTTGTGCCGGGGTAAAAAGAGCTGTGGACATATTAGCTATTACTTTAGA
AAAATACAAAAACGAGCGCCAAGTTTATGTGCTACACGAAATTGTTCACAATAAATATATAGTAGAGGACTTTAAGAAAC
AAGGAGTGGTTTTTGTAAGCAGTATCAAGGAAATTGAAGATAATGGAGGAATACTGATCTTCAGCGCGCACGGAGTGTCA
AAAAATATAGAAGATGAGGCAAAAAGAAAGGGTATTCAAGTGATTGATGCAACATGTCCTTTAGTCAACAAAGTGCATAA
AGAAGCAAAAAGGTATGAAAACAGTGGTAAAGAATTAATTCTCATTGGGCATGAAAACCATCCAGAAGTTAAGGGAATTA
GGGGAAGAGTAAACAATCCTATCACTTTAGTGCAAACTTTGCAGGATGTATATGATTTAAAGGTTAAAGATCCAGATAAT
TTATCTTATGTTACACAAACCACGTTAAGCATCGACGATACCCGTGACATTATTGCTGCCCTAAAATTAAGGTTTCCAGG
CATAACAGGTCCAGATTTAAAGGACATATGCTATGCAACGCAAAATAGACAAAATGCTGTTAAAAAGCTAGCTGAAATTG
TAGACATGGTATTAGTTGTAGGAAGTAAAAATAGCTCAAATTCAAACCGCTTATTAGATCTGTGCACTGCTAGAGGAAAA
AGAGCCTACTTGATTGATAATTACAGATGTGTGAATAAAAGCTGGTTTCAGGGCGTAGAAAAGATAGGGATCACTGCAGG
TGCTTCTGCTCCTGACATATTAGTTGATGAATTAATGGACTACCTAAAAGTAAGCATGAATGTAGAAGTTTCAGTTATGC
CAGGTGGTATTATTGAAAATGTTCAGTTTAAAATACCGAATCTGGTTTAA

Upstream 100 bases:

>100_bases
AAAATAATTATTATATAAAATATAAATAATGGTACTATAAAACACAGTGAGCGGAGCTTATTTTTAAGTAATGCTTGCAT
ATTTACTTGATTTGACAAAA

Downstream 100 bases:

>100_bases
AACACAACTTATCTACTAAGCTTCTCTTGAAATCACACAGACCAGCATCTATAGCTAAGACGTCATGCCGCGATTCATTA
GACTTCTTGCATAACCATAT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MEIILAEPRGFCAGVKRAVDILAITLEKYKNERQVYVLHEIVHNKYIVEDFKKQGVVFVSSIKEIEDNGGILIFSAHGVS
KNIEDEAKRKGIQVIDATCPLVNKVHKEAKRYENSGKELILIGHENHPEVKGIRGRVNNPITLVQTLQDVYDLKVKDPDN
LSYVTQTTLSIDDTRDIIAALKLRFPGITGPDLKDICYATQNRQNAVKKLAEIVDMVLVVGSKNSSNSNRLLDLCTARGK
RAYLIDNYRCVNKSWFQGVEKIGITAGASAPDILVDELMDYLKVSMNVEVSVMPGGIIENVQFKIPNLV

Sequences:

>Translated_309_residues
MEIILAEPRGFCAGVKRAVDILAITLEKYKNERQVYVLHEIVHNKYIVEDFKKQGVVFVSSIKEIEDNGGILIFSAHGVS
KNIEDEAKRKGIQVIDATCPLVNKVHKEAKRYENSGKELILIGHENHPEVKGIRGRVNNPITLVQTLQDVYDLKVKDPDN
LSYVTQTTLSIDDTRDIIAALKLRFPGITGPDLKDICYATQNRQNAVKKLAEIVDMVLVVGSKNSSNSNRLLDLCTARGK
RAYLIDNYRCVNKSWFQGVEKIGITAGASAPDILVDELMDYLKVSMNVEVSVMPGGIIENVQFKIPNLV
>Mature_309_residues
MEIILAEPRGFCAGVKRAVDILAITLEKYKNERQVYVLHEIVHNKYIVEDFKKQGVVFVSSIKEIEDNGGILIFSAHGVS
KNIEDEAKRKGIQVIDATCPLVNKVHKEAKRYENSGKELILIGHENHPEVKGIRGRVNNPITLVQTLQDVYDLKVKDPDN
LSYVTQTTLSIDDTRDIIAALKLRFPGITGPDLKDICYATQNRQNAVKKLAEIVDMVLVVGSKNSSNSNRLLDLCTARGK
RAYLIDNYRCVNKSWFQGVEKIGITAGASAPDILVDELMDYLKVSMNVEVSVMPGGIIENVQFKIPNLV

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=51.4563106796116, Blast_Score=313, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 34475; Mature: 34475

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIILAEPRGFCAGVKRAVDILAITLEKYKNERQVYVLHEIVHNKYIVEDFKKQGVVFVS
CEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCHHHHHHHHHCCEEEEE
SIKEIEDNGGILIFSAHGVSKNIEDEAKRKGIQVIDATCPLVNKVHKEAKRYENSGKELI
HHHHHCCCCCEEEEECCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCEEE
LIGHENHPEVKGIRGRVNNPITLVQTLQDVYDLKVKDPDNLSYVTQTTLSIDDTRDIIAA
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCEECCCCCCCEEEEEEEEECCCHHHHHHH
LKLRFPGITGPDLKDICYATQNRQNAVKKLAEIVDMVLVVGSKNSSNSNRLLDLCTARGK
HHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHCCCC
RAYLIDNYRCVNKSWFQGVEKIGITAGASAPDILVDELMDYLKVSMNVEVSVMPGGIIEN
EEEEECCEEECCHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCC
VQFKIPNLV
CEEECCCCC
>Mature Secondary Structure
MEIILAEPRGFCAGVKRAVDILAITLEKYKNERQVYVLHEIVHNKYIVEDFKKQGVVFVS
CEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCHHHHHHHHHCCEEEEE
SIKEIEDNGGILIFSAHGVSKNIEDEAKRKGIQVIDATCPLVNKVHKEAKRYENSGKELI
HHHHHCCCCCEEEEECCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCEEE
LIGHENHPEVKGIRGRVNNPITLVQTLQDVYDLKVKDPDNLSYVTQTTLSIDDTRDIIAA
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCEECCCCCCCEEEEEEEEECCCHHHHHHH
LKLRFPGITGPDLKDICYATQNRQNAVKKLAEIVDMVLVVGSKNSSNSNRLLDLCTARGK
HHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHCCCC
RAYLIDNYRCVNKSWFQGVEKIGITAGASAPDILVDELMDYLKVSMNVEVSVMPGGIIEN
EEEEECCEEECCHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCC
VQFKIPNLV
CEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA