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The map label for this gene is rnc

Identifier: 225630900

GI number: 225630900

Start: 1308327

End: 1309025

Strand: Direct

Name: rnc

Synonym: WRi_012160

Alternate gene names: 225630900

Gene position: 1308327-1309025 (Clockwise)

Preceding gene: 225630899

Following gene: 225630904

Centisome position: 90.49

GC content: 35.48

Gene sequence:

>699_bases
ATGAAGAGTTTGAATGATGCAATATCTAAGATCATTGATTATAAATTTACAAATTATGCAATATTAGAAGAGGCACTAAC
TCACCCAAGCGTAAATAAAAGGAATAGCAAAAACCAGATTGTAAGCTACGAAAGACTAGAGTTTTTGGGCGATAGTGTTT
TGAATATGGTTGTATCTGCCACACTGTTTAAACTATTTCCTGAAGAAAAAGAAGGAGCATTGGCAAAAAGAAAGACGGAT
TTAGTTTGTGGCAACACCATTGCTAATGTTGCTAAAGAAATAAAATTAGGCAGCTTTATCATCATGAATAATAGTGAACG
CTGCAATGGAGGGAGATGTAACTTAAAAAATTTAGAAAACTCGCTTGAAGCACTAATAGGTGCAATTTATATTGATGGCG
GGCTTGAAAATGTTGAAAAATTTATTATCCAATATTGGGAAAAGCTAGCTAAAGGCATGCTTGACCCCCCTCAAGATCCT
AAAACCTCACTGCAAGAGTGGACTCAGAAAAACAAATTGCCTTTACCAGAGTATGAGCTTGTAAAACAAACTGGACCAGC
GCACAATCCTGAATTTACTATATCAGTTTGCATAGAAGATTATGGTAAAGTTTCTGCATGCGCTTCTAGTAAAAAGATTG
CTGAACAAAAAGCTGCTGAGTTGATGCTAGAAAAAATTGGAAAAGACGCTTCAGTTTAG

Upstream 100 bases:

>100_bases
TATACATTAATTTTGGAAGTTGGTGGTATTATTTTAATAAAATAAAACTAATTCTCTGGTATAAAGGTGGTAGAAAGTGC
TTTTCTTTTAAATAATTGGT

Downstream 100 bases:

>100_bases
GCAAAATAGGCATTAATGTAAATTTTAAGGAAGCTAATAGCGTTTAGGGTAAATGGTCAATTTAAGGAGAAGCATAGTAT
TATTCATAAAAAAGTTGATC

Product: ribonuclease III

Products: NA

Alternate protein names: Ribonuclease III; RNase III

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MKSLNDAISKIIDYKFTNYAILEEALTHPSVNKRNSKNQIVSYERLEFLGDSVLNMVVSATLFKLFPEEKEGALAKRKTD
LVCGNTIANVAKEIKLGSFIIMNNSERCNGGRCNLKNLENSLEALIGAIYIDGGLENVEKFIIQYWEKLAKGMLDPPQDP
KTSLQEWTQKNKLPLPEYELVKQTGPAHNPEFTISVCIEDYGKVSACASSKKIAEQKAAELMLEKIGKDASV

Sequences:

>Translated_232_residues
MKSLNDAISKIIDYKFTNYAILEEALTHPSVNKRNSKNQIVSYERLEFLGDSVLNMVVSATLFKLFPEEKEGALAKRKTD
LVCGNTIANVAKEIKLGSFIIMNNSERCNGGRCNLKNLENSLEALIGAIYIDGGLENVEKFIIQYWEKLAKGMLDPPQDP
KTSLQEWTQKNKLPLPEYELVKQTGPAHNPEFTISVCIEDYGKVSACASSKKIAEQKAAELMLEKIGKDASV
>Mature_232_residues
MKSLNDAISKIIDYKFTNYAILEEALTHPSVNKRNSKNQIVSYERLEFLGDSVLNMVVSATLFKLFPEEKEGALAKRKTD
LVCGNTIANVAKEIKLGSFIIMNNSERCNGGRCNLKNLENSLEALIGAIYIDGGLENVEKFIIQYWEKLAKGMLDPPQDP
KTSLQEWTQKNKLPLPEYELVKQTGPAHNPEFTISVCIEDYGKVSACASSKKIAEQKAAELMLEKIGKDASV

Specific function: Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs

COG id: COG0571

COG function: function code K; dsRNA-specific ribonuclease

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase III domain

Homologues:

Organism=Homo sapiens, GI155030234, Length=192, Percent_Identity=33.3333333333333, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI155030236, Length=192, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=2e-18,
Organism=Escherichia coli, GI1788920, Length=210, Percent_Identity=35.2380952380952, Blast_Score=137, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17506883, Length=228, Percent_Identity=31.140350877193, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI193202651, Length=228, Percent_Identity=31.140350877193, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17137682, Length=221, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNC_WOLPM (Q73FT3)

Other databases:

- EMBL:   AE017196
- RefSeq:   NP_966952.1
- ProteinModelPortal:   Q73FT3
- SMR:   Q73FT3
- EnsemblBacteria:   EBWOLT00000004742
- GeneID:   2738188
- GenomeReviews:   AE017196_GR
- KEGG:   wol:WD1240
- TIGR:   WD_1240
- GeneTree:   EBGT00050000030997
- HOGENOM:   HBG744556
- OMA:   ALTHKSF
- PhylomeDB:   Q73FT3
- ProtClustDB:   PRK00102
- BioCyc:   WPIP955:WD_1240-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00104
- InterPro:   IPR001159
- InterPro:   IPR014720
- InterPro:   IPR000999
- InterPro:   IPR011907
- Gene3D:   G3DSA:3.30.160.20
- Gene3D:   G3DSA:1.10.1520.10
- SMART:   SM00358
- SMART:   SM00535
- TIGRFAMs:   TIGR02191

Pfam domain/function: PF00035 dsrm; PF00636 Ribonuclease_3; SSF69065 RNase_III

EC number: =3.1.26.3

Molecular weight: Translated: 25848; Mature: 25848

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50137 DS_RBD; PS00517 RNASE_3_1; PS50142 RNASE_3_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLNDAISKIIDYKFTNYAILEEALTHPSVNKRNSKNQIVSYERLEFLGDSVLNMVVSA
CCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLFKLFPEEKEGALAKRKTDLVCGNTIANVAKEIKLGSFIIMNNSERCNGGRCNLKNLEN
HHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHH
SLEALIGAIYIDGGLENVEKFIIQYWEKLAKGMLDPPQDPKTSLQEWTQKNKLPLPEYEL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHH
VKQTGPAHNPEFTISVCIEDYGKVSACASSKKIAEQKAAELMLEKIGKDASV
HHHCCCCCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKSLNDAISKIIDYKFTNYAILEEALTHPSVNKRNSKNQIVSYERLEFLGDSVLNMVVSA
CCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLFKLFPEEKEGALAKRKTDLVCGNTIANVAKEIKLGSFIIMNNSERCNGGRCNLKNLEN
HHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHH
SLEALIGAIYIDGGLENVEKFIIQYWEKLAKGMLDPPQDPKTSLQEWTQKNKLPLPEYEL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHH
VKQTGPAHNPEFTISVCIEDYGKVSACASSKKIAEQKAAELMLEKIGKDASV
HHHCCCCCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA