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The map label for this gene is clpA [C]

Identifier: 225630896

GI number: 225630896

Start: 1302640

End: 1304946

Strand: Direct

Name: clpA [C]

Synonym: WRi_012110

Alternate gene names: 225630896

Gene position: 1302640-1304946 (Clockwise)

Preceding gene: 225630895

Following gene: 225630897

Centisome position: 90.09

GC content: 33.94

Gene sequence:

>2307_bases
ATGATTTCCAAAAACTTAGAGGCAAGTTTGAATAGAGCACTATTCATTGCTTCTAATTTTAATCTTAAATATGCGAAAGT
AGAACATTTATTGCTGGCGTTAACTAAAGACGTGGATGTAAATTACGTTTTATCAAGATGTAATATCAGAGCCGATGAAA
TCATCAGTATGGATAACATTCTATCAAGATGCAATATTAAGGCTAATGATTATAATAATAATATAAAAGGGTTTTTACAA
AGCAGTTCTGAATTGATTATCAGTGGAGTTAAACCTAGCTCAATGTTTCAATGCATAATACACAGAGCAATAATACGGGC
TCATAGCTTGGGGAAAAAAGAAATAAATGGAGCAAGTGTTCTAGTAGAAATTTTGTCTGGGCAAGATTTATACGTTGAAG
ATCTATTGCAGAAACAAAGTGCGAAAGATTCCAATTTGATTTACCACATATCTAACATGAAATACTCTAACGATATAGAT
GAATACACCACTAATCATAAAGTAAAACTTGATAAAAATAATGATGCTCCTACTACAGTAAATAAAGGTGAGCTACTAAA
AGATGAGGAAATTCTACAAAGTTATTGTAAAAATTTAAATGACTATGCAAGAAGCAAAAAAATAGATTATGTTATTGGTC
GTGATTATGAATTAAATCGCACTATAGAAATATTATTGAGGCGTAGAAAAAACAACCCTTTATATGTTGGAGATCCAGGT
GTTGGCAAAACCACAATAGTTGAGGGTTTGGTACTCAAAATCATTGAAGGCAGTGTTCCGAGTGTGCTCAGGTCCAGTAT
AATTTATGCTTTGGATTTAGGATCACTCCTTGCAGGGACACGCTACAGAGGTGATTTTGAAGAGAGAATAAAATCCATAA
TAAAAGCGATTGAGGCAAAACCAGGTGCTATCCTTTTTATTGACGAAATACACACTATCATTGGAGCAGGTTCTACAAGT
GGTAGCTTTCTTGATGCCGGTAATCTGCTCAAACCTGCACTTGCAAGAGGTACACTGCGTTGTATAGGTGCAACTACATA
TAGAGAATACAGCACTAGTTTTGAAAAAGATAAAGCACTAGCGAGAAGGTTTCAAAAAATTAATGTTAAAGAATCTTCTG
TTAATGAAACGATAAAGATACTAGATGGTATAAAGCACTACTATGAAGGGTATCATGGAGTATATTATACAAAGAATGCC
ATTAGGTCTGCGGCTGAACTTTCGCATAAGTATATTACTGGGCGAATATTACCTGATAAAGCGGTTGATGTTATTGATGA
GGCAGGGGCATATTGTAAATTGCTAAGAAACAGGGGTAAAATTGTAAATAGTAGAGATATTAAGAATACCATTACTAGAA
TTACAAATGTGCCTTGCGGATCTGAATCTGATGATTTGCAGAAAGTAAAGTCTTTAAAAGCTAATCTAGAGAAGGTGATT
TTTGGCCAAGAGCAGGCAATAGAATCTCTTGTTAATTCTATTAAAATTGCTAAATCTGGGTTGAGAAATTACAATAAGCC
TTTAGCAAATTATCTTTTTGCAGGGCCAACCGGTGTTGGTAAAACCGAGCTAGCAAAACAATTAGCAGAAAGCATGGGCA
TGAATCTTATACGCTTTGATATGTCCGAATATATTGAGTCTCATACGATATCAAGGATGATTGGTTCTCCTCCTGGATAT
GTAGGTTATGATCAGGGTGGATTACTCACAGAGTCTGTATCTAATAATCAATATAGTGTTGTGCTGCTTGATGAAATTGA
AAAGGCTCATAGCGATATCTATAATATATTGCTACAAATTATGGATTATGGTTGCGTTACAGACACTTACGGACGTAAAG
TTAACTTTTCCAATATAATCTTAATTATGACAACTAATGCAGGAGCAGCTGAACGCAGCAAAAGTTTTGTTGGCTTTGGG
CATAAAAAATTTAACACTGGTGATAGTAAAAAAGCGATAGAACAGGTTTTTAGCCCTGAATTTCGTAATCGTCTTGATGC
GATTATTTCTTTTTCTGACTTAAATGCGGATATAATTCTGCATATTGTAGATAAATTTATTCAGGAACTGAAAAAGCAGC
TGACGCAAAAAGGCATAAACTGCTTGGTAGAAGATGAAGTGAAATCTTATCTTGCACAAACAGGTTATAGTAAGGAAATG
GGAGCACGTCCGATAGAGAGACTTATTGAAAAAGAGATAAAAAGTTACTTAGCGGAAGAAATACTGAATCGTAAATTAAT
AAAAGGAAAGAAATTAAGAATCTATATGAATAAAGTAGAAAATAAAATTGCTTTTGATATAGTTTGA

Upstream 100 bases:

>100_bases
GGGATTCAAATTGGCTACAGCGAACTGCTGGATTTCAAATTTTTTAATAATTGTGTGCTATTCATTATATATGAGTTCCC
ACTTTTAAAGAGAATAGGAG

Downstream 100 bases:

>100_bases
AATTCTTGTTTTTTTATTACACTGATAGTATACTTATTTTAAGAATAATGAGGCTATAGTGGCAAGTTTAACTAGAAATC
AGTATTACAGTAATCGCGAT

Product: ATP-dependent Clp protease, ATP-binding subunit ClpA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 768; Mature: 768

Protein sequence:

>768_residues
MISKNLEASLNRALFIASNFNLKYAKVEHLLLALTKDVDVNYVLSRCNIRADEIISMDNILSRCNIKANDYNNNIKGFLQ
SSSELIISGVKPSSMFQCIIHRAIIRAHSLGKKEINGASVLVEILSGQDLYVEDLLQKQSAKDSNLIYHISNMKYSNDID
EYTTNHKVKLDKNNDAPTTVNKGELLKDEEILQSYCKNLNDYARSKKIDYVIGRDYELNRTIEILLRRRKNNPLYVGDPG
VGKTTIVEGLVLKIIEGSVPSVLRSSIIYALDLGSLLAGTRYRGDFEERIKSIIKAIEAKPGAILFIDEIHTIIGAGSTS
GSFLDAGNLLKPALARGTLRCIGATTYREYSTSFEKDKALARRFQKINVKESSVNETIKILDGIKHYYEGYHGVYYTKNA
IRSAAELSHKYITGRILPDKAVDVIDEAGAYCKLLRNRGKIVNSRDIKNTITRITNVPCGSESDDLQKVKSLKANLEKVI
FGQEQAIESLVNSIKIAKSGLRNYNKPLANYLFAGPTGVGKTELAKQLAESMGMNLIRFDMSEYIESHTISRMIGSPPGY
VGYDQGGLLTESVSNNQYSVVLLDEIEKAHSDIYNILLQIMDYGCVTDTYGRKVNFSNIILIMTTNAGAAERSKSFVGFG
HKKFNTGDSKKAIEQVFSPEFRNRLDAIISFSDLNADIILHIVDKFIQELKKQLTQKGINCLVEDEVKSYLAQTGYSKEM
GARPIERLIEKEIKSYLAEEILNRKLIKGKKLRIYMNKVENKIAFDIV

Sequences:

>Translated_768_residues
MISKNLEASLNRALFIASNFNLKYAKVEHLLLALTKDVDVNYVLSRCNIRADEIISMDNILSRCNIKANDYNNNIKGFLQ
SSSELIISGVKPSSMFQCIIHRAIIRAHSLGKKEINGASVLVEILSGQDLYVEDLLQKQSAKDSNLIYHISNMKYSNDID
EYTTNHKVKLDKNNDAPTTVNKGELLKDEEILQSYCKNLNDYARSKKIDYVIGRDYELNRTIEILLRRRKNNPLYVGDPG
VGKTTIVEGLVLKIIEGSVPSVLRSSIIYALDLGSLLAGTRYRGDFEERIKSIIKAIEAKPGAILFIDEIHTIIGAGSTS
GSFLDAGNLLKPALARGTLRCIGATTYREYSTSFEKDKALARRFQKINVKESSVNETIKILDGIKHYYEGYHGVYYTKNA
IRSAAELSHKYITGRILPDKAVDVIDEAGAYCKLLRNRGKIVNSRDIKNTITRITNVPCGSESDDLQKVKSLKANLEKVI
FGQEQAIESLVNSIKIAKSGLRNYNKPLANYLFAGPTGVGKTELAKQLAESMGMNLIRFDMSEYIESHTISRMIGSPPGY
VGYDQGGLLTESVSNNQYSVVLLDEIEKAHSDIYNILLQIMDYGCVTDTYGRKVNFSNIILIMTTNAGAAERSKSFVGFG
HKKFNTGDSKKAIEQVFSPEFRNRLDAIISFSDLNADIILHIVDKFIQELKKQLTQKGINCLVEDEVKSYLAQTGYSKEM
GARPIERLIEKEIKSYLAEEILNRKLIKGKKLRIYMNKVENKIAFDIV
>Mature_768_residues
MISKNLEASLNRALFIASNFNLKYAKVEHLLLALTKDVDVNYVLSRCNIRADEIISMDNILSRCNIKANDYNNNIKGFLQ
SSSELIISGVKPSSMFQCIIHRAIIRAHSLGKKEINGASVLVEILSGQDLYVEDLLQKQSAKDSNLIYHISNMKYSNDID
EYTTNHKVKLDKNNDAPTTVNKGELLKDEEILQSYCKNLNDYARSKKIDYVIGRDYELNRTIEILLRRRKNNPLYVGDPG
VGKTTIVEGLVLKIIEGSVPSVLRSSIIYALDLGSLLAGTRYRGDFEERIKSIIKAIEAKPGAILFIDEIHTIIGAGSTS
GSFLDAGNLLKPALARGTLRCIGATTYREYSTSFEKDKALARRFQKINVKESSVNETIKILDGIKHYYEGYHGVYYTKNA
IRSAAELSHKYITGRILPDKAVDVIDEAGAYCKLLRNRGKIVNSRDIKNTITRITNVPCGSESDDLQKVKSLKANLEKVI
FGQEQAIESLVNSIKIAKSGLRNYNKPLANYLFAGPTGVGKTELAKQLAESMGMNLIRFDMSEYIESHTISRMIGSPPGY
VGYDQGGLLTESVSNNQYSVVLLDEIEKAHSDIYNILLQIMDYGCVTDTYGRKVNFSNIILIMTTNAGAAERSKSFVGFG
HKKFNTGDSKKAIEQVFSPEFRNRLDAIISFSDLNADIILHIVDKFIQELKKQLTQKGINCLVEDEVKSYLAQTGYSKEM
GARPIERLIEKEIKSYLAEEILNRKLIKGKKLRIYMNKVENKIAFDIV

Specific function: ATP-Dependent Specificity Component Of The Clpp Protease. It Directs The Protease To Specific Substrates. The Primary Function Of The Clpa-Clpp Complex Appears To Be The Degradation Of Unfolded Or Abnormal Proteins. [C]

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=324, Percent_Identity=32.7160493827161, Blast_Score=157, Evalue=4e-38,
Organism=Escherichia coli, GI1787109, Length=768, Percent_Identity=46.3541666666667, Blast_Score=699, Evalue=0.0,
Organism=Escherichia coli, GI1788943, Length=246, Percent_Identity=54.0650406504065, Blast_Score=273, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6320464, Length=244, Percent_Identity=53.2786885245902, Blast_Score=253, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6323002, Length=249, Percent_Identity=46.5863453815261, Blast_Score=234, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR013461
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 86076; Mature: 86076

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISKNLEASLNRALFIASNFNLKYAKVEHLLLALTKDVDVNYVLSRCNIRADEIISMDNI
CCCCCHHHHCCCEEEEEECCCEEHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHH
LSRCNIKANDYNNNIKGFLQSSSELIISGVKPSSMFQCIIHRAIIRAHSLGKKEINGASV
HHHCCCCCCCCCCCHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LVEILSGQDLYVEDLLQKQSAKDSNLIYHISNMKYSNDIDEYTTNHKVKLDKNNDAPTTV
HHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHCCCCEEEEECCCCCCCCC
NKGELLKDEEILQSYCKNLNDYARSKKIDYVIGRDYELNRTIEILLRRRKNNPLYVGDPG
CCCCCCCHHHHHHHHHHCHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCCEEECCCC
VGKTTIVEGLVLKIIEGSVPSVLRSSIIYALDLGSLLAGTRYRGDFEERIKSIIKAIEAK
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
PGAILFIDEIHTIIGAGSTSGSFLDAGNLLKPALARGTLRCIGATTYREYSTSFEKDKAL
CCEEEEHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCHHHCCHHHHHHHCCHHHHHHH
ARRFQKINVKESSVNETIKILDGIKHYYEGYHGVYYTKNAIRSAAELSHKYITGRILPDK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHEEEECCHH
AVDVIDEAGAYCKLLRNRGKIVNSRDIKNTITRITNVPCGSESDDLQKVKSLKANLEKVI
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
FGQEQAIESLVNSIKIAKSGLRNYNKPLANYLFAGPTGVGKTELAKQLAESMGMNLIRFD
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCHHHHHHHHHHHHCCEEEEHH
MSEYIESHTISRMIGSPPGYVGYDQGGLLTESVSNNQYSVVLLDEIEKAHSDIYNILLQI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHH
MDYGCVTDTYGRKVNFSNIILIMTTNAGAAERSKSFVGFGHKKFNTGDSKKAIEQVFSPE
HHCCCCCCCCCCEECCCCEEEEEECCCCCHHHCCCHHCCCCCCCCCCCHHHHHHHHHCHH
FRNRLDAIISFSDLNADIILHIVDKFIQELKKQLTQKGINCLVEDEVKSYLAQTGYSKEM
HHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCHHC
GARPIERLIEKEIKSYLAEEILNRKLIKGKKLRIYMNKVENKIAFDIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHCCCEEEECC
>Mature Secondary Structure
MISKNLEASLNRALFIASNFNLKYAKVEHLLLALTKDVDVNYVLSRCNIRADEIISMDNI
CCCCCHHHHCCCEEEEEECCCEEHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHH
LSRCNIKANDYNNNIKGFLQSSSELIISGVKPSSMFQCIIHRAIIRAHSLGKKEINGASV
HHHCCCCCCCCCCCHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LVEILSGQDLYVEDLLQKQSAKDSNLIYHISNMKYSNDIDEYTTNHKVKLDKNNDAPTTV
HHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHCCCCEEEEECCCCCCCCC
NKGELLKDEEILQSYCKNLNDYARSKKIDYVIGRDYELNRTIEILLRRRKNNPLYVGDPG
CCCCCCCHHHHHHHHHHCHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCCEEECCCC
VGKTTIVEGLVLKIIEGSVPSVLRSSIIYALDLGSLLAGTRYRGDFEERIKSIIKAIEAK
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
PGAILFIDEIHTIIGAGSTSGSFLDAGNLLKPALARGTLRCIGATTYREYSTSFEKDKAL
CCEEEEHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCHHHCCHHHHHHHCCHHHHHHH
ARRFQKINVKESSVNETIKILDGIKHYYEGYHGVYYTKNAIRSAAELSHKYITGRILPDK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHEEEECCHH
AVDVIDEAGAYCKLLRNRGKIVNSRDIKNTITRITNVPCGSESDDLQKVKSLKANLEKVI
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
FGQEQAIESLVNSIKIAKSGLRNYNKPLANYLFAGPTGVGKTELAKQLAESMGMNLIRFD
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCHHHHHHHHHHHHCCEEEEHH
MSEYIESHTISRMIGSPPGYVGYDQGGLLTESVSNNQYSVVLLDEIEKAHSDIYNILLQI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHH
MDYGCVTDTYGRKVNFSNIILIMTTNAGAAERSKSFVGFGHKKFNTGDSKKAIEQVFSPE
HHCCCCCCCCCCEECCCCEEEEEECCCCCHHHCCCHHCCCCCCCCCCCHHHHHHHHHCHH
FRNRLDAIISFSDLNADIILHIVDKFIQELKKQLTQKGINCLVEDEVKSYLAQTGYSKEM
HHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCHHC
GARPIERLIEKEIKSYLAEEILNRKLIKGKKLRIYMNKVENKIAFDIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6209404 [H]