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The map label for this gene is pmbA [H]

Identifier: 225630893

GI number: 225630893

Start: 1299414

End: 1300748

Strand: Direct

Name: pmbA [H]

Synonym: WRi_012080

Alternate gene names: 225630893

Gene position: 1299414-1300748 (Clockwise)

Preceding gene: 225630892

Following gene: 225630894

Centisome position: 89.87

GC content: 34.23

Gene sequence:

>1335_bases
ATGAATATATTAAATATTGCAGCAGATATTACTAAACTAATAAAAAAGCAAAATCAAGATGCGGAAGTTACAATATATGA
AACTAATAAGACTTCAGTTTCTCAGCGTCTATCAAAAATTGAGCAAATATCACAATCTAAAAATTGCACTGTAGGAATCA
GAGCTATAGCAGGTAAAAACAAAGCTGCGTATATTTCTACAAACGATTTGAATAATCTGAGTGATACAGTAAGCCAAGTG
GTAGAAATGGCAAAGAATGCACCAGAAGATCCTTGCATTAATTTTGCTGTAAATGGTAGTAATTATATCTCTTCTGCAGA
TTTAAGTATCTCAGATAATAATGTTGTAACCGTTGATAACCTAAAAGAAATTGCTGAAGCTGCAGAAAATTCAGCCCTTG
CACATAAGAGTATCACTAATTCTGAAGGAGCCTCTTCTTCATACGCTTTAGTGAATACAGTATTATCGACTGTTTCTGGT
TTTATTGGCTCGTTTAGTAAATCAACCTTTGCTAATCAGGTCTCTGTTGTTGCTGGAAGAGAAAGTGAAATGAAGGTTGG
TTACGATTATGATATAGCATGTAATTTTAGTGATTTAAAGTTGCCAGAGTTAATGGGAAAAGAAGCAGCAAAAAGAGCAG
TTGATCAATTGAATTCACGTACAATGAAAACTGGTAAATTTCCAGTTATTTTTGAAAAAAGAGCAGCAAAAGGGCTAGTA
AAAAGTTTTGCTTCTGCTATAAATGGGAGCAGTATTATAAGCAACAGTTCTTTCTTGAGGGGTAGTTTAAACGCTCAGAT
TTTCAACGATAGAATTAATATTATCGATGATCCATTGTTGCCAAGAGGCATAGCATCGAGACCATTTGATGGAGAAGGGA
TAACTAGCAAGAAAAATATATTTGTAAAAAACGGAATATTACAAAATTGGATTTTAGACTTATACTCGGCTAGAAAATTA
GACTCAGAAACAACCGGAAGTGCAACTCGTGCAAGTAATGCTGCAGTTATTCCTGCAGCTAGTAACCTCTACATTGAGAA
TGGTGATGTATCGTTTGAAGAATTAATTCAGGAAGTGAAAGAGGGAATATATGTAACTGATTTATTTGGTTTTGGTGTCA
ATTTAATCAATGGCGATTACAGCCAAGGCGCTTCCGGATTTTTTATAGAAAATGGAAAAATAACGTATCCAATACATGAG
ATTACCATTGCTAGCAATTTGAAGGATATGTTTAGCAATTTAGTCGTTGCAGATGATCTAACTTTTTGTGGGCAATTTAA
TTCACCAACGATTAAAGTTAGCGAAATGACGGTTGCAGGTTCGCTTAACGATTAA

Upstream 100 bases:

>100_bases
AGCATTGCTTTATAATCGTTCTCGTCAAGCAGCGATTACGACTGATTTAATTGAAGTTATAGGTGGTGCAGAATCTTTAT
AGAAATCTAAGGAATAGAAA

Downstream 100 bases:

>100_bases
AAAATTTATTTGCATCAAGTACAACAATTGTGTATTATTGTCATGTAATGTTAAGAATAGACTATGGAGTATAAAGGTTT
AGTTTCAATAGCTAAGCTTA

Product: pmbA protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MNILNIAADITKLIKKQNQDAEVTIYETNKTSVSQRLSKIEQISQSKNCTVGIRAIAGKNKAAYISTNDLNNLSDTVSQV
VEMAKNAPEDPCINFAVNGSNYISSADLSISDNNVVTVDNLKEIAEAAENSALAHKSITNSEGASSSYALVNTVLSTVSG
FIGSFSKSTFANQVSVVAGRESEMKVGYDYDIACNFSDLKLPELMGKEAAKRAVDQLNSRTMKTGKFPVIFEKRAAKGLV
KSFASAINGSSIISNSSFLRGSLNAQIFNDRINIIDDPLLPRGIASRPFDGEGITSKKNIFVKNGILQNWILDLYSARKL
DSETTGSATRASNAAVIPAASNLYIENGDVSFEELIQEVKEGIYVTDLFGFGVNLINGDYSQGASGFFIENGKITYPIHE
ITIASNLKDMFSNLVVADDLTFCGQFNSPTIKVSEMTVAGSLND

Sequences:

>Translated_444_residues
MNILNIAADITKLIKKQNQDAEVTIYETNKTSVSQRLSKIEQISQSKNCTVGIRAIAGKNKAAYISTNDLNNLSDTVSQV
VEMAKNAPEDPCINFAVNGSNYISSADLSISDNNVVTVDNLKEIAEAAENSALAHKSITNSEGASSSYALVNTVLSTVSG
FIGSFSKSTFANQVSVVAGRESEMKVGYDYDIACNFSDLKLPELMGKEAAKRAVDQLNSRTMKTGKFPVIFEKRAAKGLV
KSFASAINGSSIISNSSFLRGSLNAQIFNDRINIIDDPLLPRGIASRPFDGEGITSKKNIFVKNGILQNWILDLYSARKL
DSETTGSATRASNAAVIPAASNLYIENGDVSFEELIQEVKEGIYVTDLFGFGVNLINGDYSQGASGFFIENGKITYPIHE
ITIASNLKDMFSNLVVADDLTFCGQFNSPTIKVSEMTVAGSLND
>Mature_444_residues
MNILNIAADITKLIKKQNQDAEVTIYETNKTSVSQRLSKIEQISQSKNCTVGIRAIAGKNKAAYISTNDLNNLSDTVSQV
VEMAKNAPEDPCINFAVNGSNYISSADLSISDNNVVTVDNLKEIAEAAENSALAHKSITNSEGASSSYALVNTVLSTVSG
FIGSFSKSTFANQVSVVAGRESEMKVGYDYDIACNFSDLKLPELMGKEAAKRAVDQLNSRTMKTGKFPVIFEKRAAKGLV
KSFASAINGSSIISNSSFLRGSLNAQIFNDRINIIDDPLLPRGIASRPFDGEGITSKKNIFVKNGILQNWILDLYSARKL
DSETTGSATRASNAAVIPAASNLYIENGDVSFEELIQEVKEGIYVTDLFGFGVNLINGDYSQGASGFFIENGKITYPIHE
ITIASNLKDMFSNLVVADDLTFCGQFNSPTIKVSEMTVAGSLND

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=435, Percent_Identity=35.4022988505747, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1789640, Length=419, Percent_Identity=24.1050119331742, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47817; Mature: 47817

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILNIAADITKLIKKQNQDAEVTIYETNKTSVSQRLSKIEQISQSKNCTVGIRAIAGKN
CCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
KAAYISTNDLNNLSDTVSQVVEMAKNAPEDPCINFAVNGSNYISSADLSISDNNVVTVDN
CEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEECCCCEECCCCEEEHHH
LKEIAEAAENSALAHKSITNSEGASSSYALVNTVLSTVSGFIGSFSKSTFANQVSVVAGR
HHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCEEEEECC
ESEMKVGYDYDIACNFSDLKLPELMGKEAAKRAVDQLNSRTMKTGKFPVIFEKRAAKGLV
CCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHH
KSFASAINGSSIISNSSFLRGSLNAQIFNDRINIIDDPLLPRGIASRPFDGEGITSKKNI
HHHHHHCCCCCEECCCCCEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
FVKNGILQNWILDLYSARKLDSETTGSATRASNAAVIPAASNLYIENGDVSFEELIQEVK
EEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCHHHHHHHHH
EGIYVTDLFGFGVNLINGDYSQGASGFFIENGKITYPIHEITIASNLKDMFSNLVVADDL
CCEEEEEHHHCCEEEECCCCCCCCCCEEEECCEEEECCEEEEEHHHHHHHHHCEEEECCH
TFCGQFNSPTIKVSEMTVAGSLND
HCCCCCCCCEEEEEEEEEECCCCC
>Mature Secondary Structure
MNILNIAADITKLIKKQNQDAEVTIYETNKTSVSQRLSKIEQISQSKNCTVGIRAIAGKN
CCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
KAAYISTNDLNNLSDTVSQVVEMAKNAPEDPCINFAVNGSNYISSADLSISDNNVVTVDN
CEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEECCCCEECCCCEEEHHH
LKEIAEAAENSALAHKSITNSEGASSSYALVNTVLSTVSGFIGSFSKSTFANQVSVVAGR
HHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCEEEEECC
ESEMKVGYDYDIACNFSDLKLPELMGKEAAKRAVDQLNSRTMKTGKFPVIFEKRAAKGLV
CCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHH
KSFASAINGSSIISNSSFLRGSLNAQIFNDRINIIDDPLLPRGIASRPFDGEGITSKKNI
HHHHHHCCCCCEECCCCCEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
FVKNGILQNWILDLYSARKLDSETTGSATRASNAAVIPAASNLYIENGDVSFEELIQEVK
EEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCHHHHHHHHH
EGIYVTDLFGFGVNLINGDYSQGASGFFIENGKITYPIHEITIASNLKDMFSNLVVADDL
CCEEEEEHHHCCEEEECCCCCCCCCCEEEECCEEEECCEEEEEHHHHHHHHHCEEEECCH
TFCGQFNSPTIKVSEMTVAGSLND
HCCCCCCCCEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]