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The map label for this gene is hemF [H]

Identifier: 225630880

GI number: 225630880

Start: 1283150

End: 1283974

Strand: Reverse

Name: hemF [H]

Synonym: WRi_011940

Alternate gene names: 225630880

Gene position: 1283974-1283150 (Counterclockwise)

Preceding gene: 225630884

Following gene: 225630879

Centisome position: 88.8

GC content: 35.64

Gene sequence:

>825_bases
ATGGAAAAACAAAAAACACAAGCATTTAAATGGTTCTGTGCACTAAGAGATAAGATCATAGAATCTTTCTTATTAATTGA
AAAACAATCATCCGCAGAGCCAAAGATCGAAAAGAGAAAGTGGGATCGCCCAGGTGGTGGAGGTGGTGAATCTACAATTA
TTTTTGGTAATGTTTTTGAAAAAGTTGGAGTAAACGTTTCAAAAGTACACGGAAAATTTGCAGATTCAGCCATCAATGAA
ATTCCTGGTGCAAGTGAAAGTAACGGAGAGTTCTGGGCAAGCGGTATATCTTTAGTGTCTCATATGCAATCGCCCCTTAT
CCCTGCAGCGCATATGAACACAAGGCTGATATATACTTCAAAACAATGGTTTGGTGGAGGAATGGATTTTACGCCAATAT
ATAGAAATGAAGAAGATTGCAAGTATATTCATGAATCAATCAAAATGATATGTGATAGATTTGACACCGGATATTATCCA
AAATTTAAAGAGCAATGTGACAATTATTTTTTCTTACAACACAGAAAAGAGCCACGTGGCATTGGTGGTATTTTCTATGA
TAATCTGAGTTCTGGTAATTGGGAAAATGATTTTGAGTTCACAAAAGCAGTAGGTGAAGCCTTTTTGGAAATTTATTTGC
ACATCATACGTAAACATATACAAAAATCTTGGACAAAAGAACAACGAGAAAATCAATTAATAAAACGTGGCAGATATGTA
GAATTCAATCTGTTATACGATCGTGGCACAAGGTTTGGTTTAATGACTGACGGCAATCCAGATGCAATTATGATGTCAAT
GCCACCACTTGTTAAGTGGTTATAG

Upstream 100 bases:

>100_bases
TAGATTTATTAACGTCTTTGATAGATAATTATCATCCAAATCTAATTAAGTTCCTTAAATATCAGATATAACAATGTTAA
TAAGCTAGCAAGAGGATATT

Downstream 100 bases:

>100_bases
AGAGCTGCTATTAGTAGAAAATAAATCATCATAGTTTAAAATTGTACCATGGTAGTGAGCTTTTATATAGGGGGTATAAT
CATGGGCATAATAGATAATT

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MEKQKTQAFKWFCALRDKIIESFLLIEKQSSAEPKIEKRKWDRPGGGGGESTIIFGNVFEKVGVNVSKVHGKFADSAINE
IPGASESNGEFWASGISLVSHMQSPLIPAAHMNTRLIYTSKQWFGGGMDFTPIYRNEEDCKYIHESIKMICDRFDTGYYP
KFKEQCDNYFFLQHRKEPRGIGGIFYDNLSSGNWENDFEFTKAVGEAFLEIYLHIIRKHIQKSWTKEQRENQLIKRGRYV
EFNLLYDRGTRFGLMTDGNPDAIMMSMPPLVKWL

Sequences:

>Translated_274_residues
MEKQKTQAFKWFCALRDKIIESFLLIEKQSSAEPKIEKRKWDRPGGGGGESTIIFGNVFEKVGVNVSKVHGKFADSAINE
IPGASESNGEFWASGISLVSHMQSPLIPAAHMNTRLIYTSKQWFGGGMDFTPIYRNEEDCKYIHESIKMICDRFDTGYYP
KFKEQCDNYFFLQHRKEPRGIGGIFYDNLSSGNWENDFEFTKAVGEAFLEIYLHIIRKHIQKSWTKEQRENQLIKRGRYV
EFNLLYDRGTRFGLMTDGNPDAIMMSMPPLVKWL
>Mature_274_residues
MEKQKTQAFKWFCALRDKIIESFLLIEKQSSAEPKIEKRKWDRPGGGGGESTIIFGNVFEKVGVNVSKVHGKFADSAINE
IPGASESNGEFWASGISLVSHMQSPLIPAAHMNTRLIYTSKQWFGGGMDFTPIYRNEEDCKYIHESIKMICDRFDTGYYP
KFKEQCDNYFFLQHRKEPRGIGGIFYDNLSSGNWENDFEFTKAVGEAFLEIYLHIIRKHIQKSWTKEQRENQLIKRGRYV
EFNLLYDRGTRFGLMTDGNPDAIMMSMPPLVKWL

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III [H]

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI41393599, Length=248, Percent_Identity=41.9354838709677, Blast_Score=204, Evalue=6e-53,
Organism=Escherichia coli, GI1788777, Length=284, Percent_Identity=39.4366197183099, Blast_Score=215, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6320249, Length=265, Percent_Identity=40, Blast_Score=193, Evalue=2e-50,
Organism=Drosophila melanogaster, GI17864388, Length=261, Percent_Identity=41.7624521072797, Blast_Score=219, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001260
- InterPro:   IPR018375 [H]

Pfam domain/function: PF01218 Coprogen_oxidas [H]

EC number: =1.3.3.3 [H]

Molecular weight: Translated: 31714; Mature: 31714

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKQKTQAFKWFCALRDKIIESFLLIEKQSSAEPKIEKRKWDRPGGGGGESTIIFGNVFE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCEEEEHHHHH
KVGVNVSKVHGKFADSAINEIPGASESNGEFWASGISLVSHMQSPLIPAAHMNTRLIYTS
HHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEC
KQWFGGGMDFTPIYRNEEDCKYIHESIKMICDRFDTGYYPKFKEQCDNYFFLQHRKEPRG
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEECCCCCC
IGGIFYDNLSSGNWENDFEFTKAVGEAFLEIYLHIIRKHIQKSWTKEQRENQLIKRGRYV
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
EFNLLYDRGTRFGLMTDGNPDAIMMSMPPLVKWL
EEEEEEECCCEEEEEECCCCCEEEEECCHHHHCC
>Mature Secondary Structure
MEKQKTQAFKWFCALRDKIIESFLLIEKQSSAEPKIEKRKWDRPGGGGGESTIIFGNVFE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCEEEEHHHHH
KVGVNVSKVHGKFADSAINEIPGASESNGEFWASGISLVSHMQSPLIPAAHMNTRLIYTS
HHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEC
KQWFGGGMDFTPIYRNEEDCKYIHESIKMICDRFDTGYYPKFKEQCDNYFFLQHRKEPRG
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEECCCCCC
IGGIFYDNLSSGNWENDFEFTKAVGEAFLEIYLHIIRKHIQKSWTKEQRENQLIKRGRYV
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
EFNLLYDRGTRFGLMTDGNPDAIMMSMPPLVKWL
EEEEEEECCCEEEEEECCCCCEEEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA