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The map label for this gene is priA [H]

Identifier: 225630867

GI number: 225630867

Start: 1268532

End: 1270874

Strand: Direct

Name: priA [H]

Synonym: WRi_011780

Alternate gene names: 225630867

Gene position: 1268532-1270874 (Clockwise)

Preceding gene: 225630865

Following gene: 225630873

Centisome position: 87.73

GC content: 35.38

Gene sequence:

>2343_bases
ATGTTAAACTCAATCACTATGGCAAAAACAGTTGACGTATTACTATCACTTCCAATTGATCAGTTATTTTCATATGCAAT
TGAGGAAAATACTGAGATTTTACTTGGAGATTACGTAGTGGTGCCATTTGGCAAAAAACGCTTAATCGGAATAGTTTGGA
AATACAGTGATAAAAGTAACCGAGAATTAAAATTTATTGAGCAAAAAATCGATTTACCAAATATTAGACCAAAATTGATT
GCATTTGCAGAGTGGGTTGCACAATACAATTTAATACCTATTGGTATGCTTGCCAAAGTGATAATGGGAGGAGTGTTAAA
AGTAAATCACATAGATAAATTGGTTTGTGTTGAACAAAAGCAGGAAACAAGTGAAATAAGTTGCCAATTAAGCCCTGAAC
AGCAGATAGCTAGTGATAAAATAATAAGCAATTTGAACGAGTATTCAGTGACTTTGCTTGACGGTGAGACAGGATCTGGA
AAAACCGAAGTTTATCTCTCTGTGATTGCACAATTGATTAAGAATTACACTGCCGTTCCAAATGCTGCTCAGACTTTACC
ATCCCAGTGCCCAGAGACACAACTGTACGAACATTGTAATTTGGGTCTACCAAGAGGGTGTTATCCGAGTAGCCTGACAC
TGGGATCCAGTTTTTCATATAACCTCATTACAAATGTTTATTTTAACTTAAAACCAATACCTAATTTACAATCAAAATTC
CTGGATTCCAGTGTCAAGCACTGGAATGACACCTTTATACTCAAGGCAAAACCCACTGTAACATTTAGCATACCGTCTTT
GCCACGTACTAGTATGACACCAGAATATGTTGAAATGACATCAGGAGAAGGTAATACACAAATTTTAATCCTCCTACCTG
AAATTGTTTTAACTTCACAATTGGTAAATCGTGTTCGCGGTCAGATATCAAAAAACTTAGTTGAATGGCACTCAGGGCTC
ACTCCAAAAACTCGCCGGAATAATTGGCTCAATATAGCAAATGGAAATGCGCAGATAATTATTGGTGCACGGTCAGCGCT
TTTTTTGCCTTATAAAAACCTAAAATTGATTATCGTTGATGAAGAGCACGACTCATCTTTTAAACAAGAACAGGGAATTA
TATATAATGCTCGAGATATGGCGATAATCTTAGCCAAATTTGAAAATATTCCGATTATTTTATCATCAGCTACTCCACTG
CTTGAAACGATTCATCATGTTAAAAACGGGAATTACAATCATGTAAAGCTAACTAAGCGATTTGGTGGTGCAGAATTGCC
ACTCATTAAAGTAGTAGATATGAGGAACAACAAGCAATGGATCTCCTCTGAGCTTTTTGAAAGCATCAAACAAACCATAG
AGAAAAAACAGCAGGTTATGCTTTTTCTAAACCGCAGAGGGTATGCACAACTGGCAATTTGTAAAAAGTGTGGATATAAA
ATTTCCTGCTTAAATTGTGCTGTTTGGCTTACATATCACAAGAAAAAAAATGCTCTTTTGTGCCACCACTGCTCTTATCA
ATTGAAACTTCCAGAAAAATGCTCTAATTGCCAAAGTGAACAGCCATTGTCCCTCTACGGCATAGGAATTGAAAGGTTAC
TTGAAGAAATGGTGAAATTAATACCAAACGCAAAAACTGCGATGATAAGCAGCGATCAGAAGTCGGTTAGTAACATCATT
GATTTAGTGTTAAAGGAAGAGGTGAACATTATAATCGGCACACAGATAATTGCTAAGGGGCACAATTTTCCCAAATTAAC
TTTGGTTGGAGTAATAAATGCAGATTTGAGCCTCGAAAATTCTGATTTAAGAGCAGCGGAAAAGACGTATCAATTATTGC
ACCAAGTTGCAGGCAGATCAGGAAGGTTTAATGAAAAAGGAATGGTAATAGTGCAAACTAATAATCCTGAAAGTTCAATA
ATAAAAGCATTGCTGCATCAAAAAAGAGACTCATTTTATGAAATCGAACTTAAGTCAAGACGTGAAGCCAAAATGCCACC
ATTTAGCAGATTAATAGCGCTTATCATTTGTGGTAAGAATCGGATTGCAACACAAAAAGCAGCGAATGAAATAGTAAAAT
CTCTTCTTTGTCATCCAAGTAGCTCAACTACTTGTATCCAGGAAAAAAATGTCAGTGCAGACAAAAAGGAATTTGAAATT
CTTGGCCCATCACCAGCAGCAATAAATTTTTTAAATAATAAGTATCGGTATAGGGTATTATTAAAAATACACAATAAACA
CAGTTTATCTATACAAAAAAAGCTGAAATATTGGATTAAAAATTGTAACTTGAATTCGAGTATTGCAGTGATAATAGATG
TTGATCCTGTGAGTTTTTTTTAA

Upstream 100 bases:

>100_bases
TACGGTCTGTAATATGAAAAATGTGTTAAACGTTAAGATTTTCTCTGTACCGATTCATCTATATTGCCAGGCTTAATAAA
GTTACAAGCTCTTTAAAACA

Downstream 100 bases:

>100_bases
CGAGTTCCGTTAAGCCTTCTTGCCAATTACTACAACATTCTCACTGGGAAAAAAATAGTCAGGTACAACCATATTTTTTG
GTGCAGGACCCCCAATCACT

Product: Primosomal protein N', superfamily II helicase

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 780; Mature: 780

Protein sequence:

>780_residues
MLNSITMAKTVDVLLSLPIDQLFSYAIEENTEILLGDYVVVPFGKKRLIGIVWKYSDKSNRELKFIEQKIDLPNIRPKLI
AFAEWVAQYNLIPIGMLAKVIMGGVLKVNHIDKLVCVEQKQETSEISCQLSPEQQIASDKIISNLNEYSVTLLDGETGSG
KTEVYLSVIAQLIKNYTAVPNAAQTLPSQCPETQLYEHCNLGLPRGCYPSSLTLGSSFSYNLITNVYFNLKPIPNLQSKF
LDSSVKHWNDTFILKAKPTVTFSIPSLPRTSMTPEYVEMTSGEGNTQILILLPEIVLTSQLVNRVRGQISKNLVEWHSGL
TPKTRRNNWLNIANGNAQIIIGARSALFLPYKNLKLIIVDEEHDSSFKQEQGIIYNARDMAIILAKFENIPIILSSATPL
LETIHHVKNGNYNHVKLTKRFGGAELPLIKVVDMRNNKQWISSELFESIKQTIEKKQQVMLFLNRRGYAQLAICKKCGYK
ISCLNCAVWLTYHKKKNALLCHHCSYQLKLPEKCSNCQSEQPLSLYGIGIERLLEEMVKLIPNAKTAMISSDQKSVSNII
DLVLKEEVNIIIGTQIIAKGHNFPKLTLVGVINADLSLENSDLRAAEKTYQLLHQVAGRSGRFNEKGMVIVQTNNPESSI
IKALLHQKRDSFYEIELKSRREAKMPPFSRLIALIICGKNRIATQKAANEIVKSLLCHPSSSTTCIQEKNVSADKKEFEI
LGPSPAAINFLNNKYRYRVLLKIHNKHSLSIQKKLKYWIKNCNLNSSIAVIIDVDPVSFF

Sequences:

>Translated_780_residues
MLNSITMAKTVDVLLSLPIDQLFSYAIEENTEILLGDYVVVPFGKKRLIGIVWKYSDKSNRELKFIEQKIDLPNIRPKLI
AFAEWVAQYNLIPIGMLAKVIMGGVLKVNHIDKLVCVEQKQETSEISCQLSPEQQIASDKIISNLNEYSVTLLDGETGSG
KTEVYLSVIAQLIKNYTAVPNAAQTLPSQCPETQLYEHCNLGLPRGCYPSSLTLGSSFSYNLITNVYFNLKPIPNLQSKF
LDSSVKHWNDTFILKAKPTVTFSIPSLPRTSMTPEYVEMTSGEGNTQILILLPEIVLTSQLVNRVRGQISKNLVEWHSGL
TPKTRRNNWLNIANGNAQIIIGARSALFLPYKNLKLIIVDEEHDSSFKQEQGIIYNARDMAIILAKFENIPIILSSATPL
LETIHHVKNGNYNHVKLTKRFGGAELPLIKVVDMRNNKQWISSELFESIKQTIEKKQQVMLFLNRRGYAQLAICKKCGYK
ISCLNCAVWLTYHKKKNALLCHHCSYQLKLPEKCSNCQSEQPLSLYGIGIERLLEEMVKLIPNAKTAMISSDQKSVSNII
DLVLKEEVNIIIGTQIIAKGHNFPKLTLVGVINADLSLENSDLRAAEKTYQLLHQVAGRSGRFNEKGMVIVQTNNPESSI
IKALLHQKRDSFYEIELKSRREAKMPPFSRLIALIICGKNRIATQKAANEIVKSLLCHPSSSTTCIQEKNVSADKKEFEI
LGPSPAAINFLNNKYRYRVLLKIHNKHSLSIQKKLKYWIKNCNLNSSIAVIIDVDPVSFF
>Mature_780_residues
MLNSITMAKTVDVLLSLPIDQLFSYAIEENTEILLGDYVVVPFGKKRLIGIVWKYSDKSNRELKFIEQKIDLPNIRPKLI
AFAEWVAQYNLIPIGMLAKVIMGGVLKVNHIDKLVCVEQKQETSEISCQLSPEQQIASDKIISNLNEYSVTLLDGETGSG
KTEVYLSVIAQLIKNYTAVPNAAQTLPSQCPETQLYEHCNLGLPRGCYPSSLTLGSSFSYNLITNVYFNLKPIPNLQSKF
LDSSVKHWNDTFILKAKPTVTFSIPSLPRTSMTPEYVEMTSGEGNTQILILLPEIVLTSQLVNRVRGQISKNLVEWHSGL
TPKTRRNNWLNIANGNAQIIIGARSALFLPYKNLKLIIVDEEHDSSFKQEQGIIYNARDMAIILAKFENIPIILSSATPL
LETIHHVKNGNYNHVKLTKRFGGAELPLIKVVDMRNNKQWISSELFESIKQTIEKKQQVMLFLNRRGYAQLAICKKCGYK
ISCLNCAVWLTYHKKKNALLCHHCSYQLKLPEKCSNCQSEQPLSLYGIGIERLLEEMVKLIPNAKTAMISSDQKSVSNII
DLVLKEEVNIIIGTQIIAKGHNFPKLTLVGVINADLSLENSDLRAAEKTYQLLHQVAGRSGRFNEKGMVIVQTNNPESSI
IKALLHQKRDSFYEIELKSRREAKMPPFSRLIALIICGKNRIATQKAANEIVKSLLCHPSSSTTCIQEKNVSADKKEFEI
LGPSPAAINFLNNKYRYRVLLKIHNKHSLSIQKKLKYWIKNCNLNSSIAVIIDVDPVSFF

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=503, Percent_Identity=36.3817097415507, Blast_Score=327, Evalue=1e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 87898; Mature: 87898

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNSITMAKTVDVLLSLPIDQLFSYAIEENTEILLGDYVVVPFGKKRLIGIVWKYSDKSN
CCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECEEEECCCCCEEEEEEEEECCCCC
RELKFIEQKIDLPNIRPKLIAFAEWVAQYNLIPIGMLAKVIMGGVLKVNHIDKLVCVEQK
CHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCEEEECCC
QETSEISCQLSPEQQIASDKIISNLNEYSVTLLDGETGSGKTEVYLSVIAQLIKNYTAVP
CCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
NAAQTLPSQCPETQLYEHCNLGLPRGCYPSSLTLGSSFSYNLITNVYFNLKPIPNLQSKF
CHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCEECCCCCCEEEEEEEEEEECCCCCHHHHH
LDSSVKHWNDTFILKAKPTVTFSIPSLPRTSMTPEYVEMTSGEGNTQILILLPEIVLTSQ
HHHHHHCCCCEEEEEECCEEEEECCCCCCCCCCCHHHEEECCCCCEEEEEEECHHHHHHH
LVNRVRGQISKNLVEWHSGLTPKTRRNNWLNIANGNAQIIIGARSALFLPYKNLKLIIVD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCEEEEEECCEEEEEEE
EEHDSSFKQEQGIIYNARDMAIILAKFENIPIILSSATPLLETIHHVKNGNYNHVKLTKR
CCCCCCHHHHCCCEEECCCEEEEEEECCCCCEEEECCCHHHHHHHHHCCCCCCEEEEEEC
FGGAELPLIKVVDMRNNKQWISSELFESIKQTIEKKQQVMLFLNRRGYAQLAICKKCGYK
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEHHHHHCCCE
ISCLNCAVWLTYHKKKNALLCHHCSYQLKLPEKCSNCQSEQPLSLYGIGIERLLEEMVKL
EEEEEEEEEEEEECCCCCEEEEECCEEEECCHHHCCCCCCCCCEEEECCHHHHHHHHHHH
IPNAKTAMISSDQKSVSNIIDLVLKEEVNIIIGTQIIAKGHNFPKLTLVGVINADLSLEN
CCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCEEEEEEEEECCCEECC
SDLRAAEKTYQLLHQVAGRSGRFNEKGMVIVQTNNPESSIIKALLHQKRDSFYEIELKSR
CCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCC
REAKMPPFSRLIALIICGKNRIATQKAANEIVKSLLCHPSSSTTCIQEKNVSADKKEFEI
CCCCCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEE
LGPSPAAINFLNNKYRYRVLLKIHNKHSLSIQKKLKYWIKNCNLNSSIAVIIDVDPVSFF
ECCCCCEEHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MLNSITMAKTVDVLLSLPIDQLFSYAIEENTEILLGDYVVVPFGKKRLIGIVWKYSDKSN
CCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECEEEECCCCCEEEEEEEEECCCCC
RELKFIEQKIDLPNIRPKLIAFAEWVAQYNLIPIGMLAKVIMGGVLKVNHIDKLVCVEQK
CHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCEEEECCC
QETSEISCQLSPEQQIASDKIISNLNEYSVTLLDGETGSGKTEVYLSVIAQLIKNYTAVP
CCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
NAAQTLPSQCPETQLYEHCNLGLPRGCYPSSLTLGSSFSYNLITNVYFNLKPIPNLQSKF
CHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCEECCCCCCEEEEEEEEEEECCCCCHHHHH
LDSSVKHWNDTFILKAKPTVTFSIPSLPRTSMTPEYVEMTSGEGNTQILILLPEIVLTSQ
HHHHHHCCCCEEEEEECCEEEEECCCCCCCCCCCHHHEEECCCCCEEEEEEECHHHHHHH
LVNRVRGQISKNLVEWHSGLTPKTRRNNWLNIANGNAQIIIGARSALFLPYKNLKLIIVD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCEEEEEECCEEEEEEE
EEHDSSFKQEQGIIYNARDMAIILAKFENIPIILSSATPLLETIHHVKNGNYNHVKLTKR
CCCCCCHHHHCCCEEECCCEEEEEEECCCCCEEEECCCHHHHHHHHHCCCCCCEEEEEEC
FGGAELPLIKVVDMRNNKQWISSELFESIKQTIEKKQQVMLFLNRRGYAQLAICKKCGYK
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEHHHHHCCCE
ISCLNCAVWLTYHKKKNALLCHHCSYQLKLPEKCSNCQSEQPLSLYGIGIERLLEEMVKL
EEEEEEEEEEEEECCCCCEEEEECCEEEECCHHHCCCCCCCCCEEEECCHHHHHHHHHHH
IPNAKTAMISSDQKSVSNIIDLVLKEEVNIIIGTQIIAKGHNFPKLTLVGVINADLSLEN
CCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCEEEEEEEEECCCEECC
SDLRAAEKTYQLLHQVAGRSGRFNEKGMVIVQTNNPESSIIKALLHQKRDSFYEIELKSR
CCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCC
REAKMPPFSRLIALIICGKNRIATQKAANEIVKSLLCHPSSSTTCIQEKNVSADKKEFEI
CCCCCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEE
LGPSPAAINFLNNKYRYRVLLKIHNKHSLSIQKKLKYWIKNCNLNSSIAVIIDVDPVSFF
ECCCCCEEHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]