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The map label for this gene is pgk [H]

Identifier: 225630837

GI number: 225630837

Start: 1230022

End: 1231218

Strand: Reverse

Name: pgk [H]

Synonym: WRi_011430

Alternate gene names: 225630837

Gene position: 1231218-1230022 (Counterclockwise)

Preceding gene: 225630845

Following gene: 225630836

Centisome position: 85.15

GC content: 36.51

Gene sequence:

>1197_bases
ATGAACATACCTAGTATAGAAAATTGTGATCTTCACAATAAAGCCGTCTTACTTAGAGTTGACTTCAATGTTCCTATAAA
AGATGGAGAAATTCGTGATGTCACTCGTATTTTGAGAGCATTGCCTACTATTCAGTATTTAGTGAATGCGAGTGCAAAGA
TTATTATTATATCACATTTTGGACGCCCAAAGGCCAGAGACAATAATCTGTCGCTAAAAAATGTAATTGACACTTTATCG
CAGTTACTAAATAAAAAAGTGAAATTCATTGATGATTGCGTTGGTGAAAAAGTGCAAAAAGCAGTGAGTGCTATGGATGC
AGGAGATATAATATTACTGGAGAATCTAAGATTTTATGAAGGGGAAAAGGAGAATGATGCAAACTTTGCCAGACAATTAG
CATCTCTGGCGGATATATATGTAAATGATGCATTTTCTTGCTCTCACAGAGCTCACGCTTCTATTTCACGCATTACAGAG
TTTTTACCTTCCTATGCAGGATTTTGCTTGCAAGATGAGCTAAAGTATCTTGAAAAAGCTGTATCGTTTAAAGCTAAACC
TATTACTGCGATAGTTGGGGGAGCTAAAATATCAACTAAAATAAAAGTGCTTATGAAGCTAACAGAAAAGGTTGATTACC
TAGTTCTAGGTGGTGCAATAGCCAACAATTTTTTGTCATTTAGCAAAGTAAATATAGGGAAATCTTTCTTTCAAAATGGT
GTTGACGACCTTCTACATAATATTGTTGAAACAGCAAATAAAAACAATTGCAAAATAGTTGTGCCAGAAGACGTTCTGGT
TGCAGTAAACTCTGATTACAGCACTAGTATTTCAAGAAAAACTGAGTCCATTTTGGACGGTGATATAATTTTGGATATCG
GATCACAAACTTTGAGTACAATAAGCAGTATAATAGCAAGCAGTAAGACTCTGCTGTGGAATGGACCTATTGGTGTTTTT
GAACATTCAGCTTTTGCAAGCGGTACAATAGGGGTAATGAAAATCGTAAGTGACTTAACGCACAAAGGAAAGTTAACCAG
CATAATAGGGGGAGGTGATAGTCTATCTGCAATAAGCGCTGCAGGCCTTACCGATAAGGATTTTACATATGTTTCCACTG
GTGGAGGAGCATTTTTAGATTGGTTAAGTGGCGATGAAATGCCGGGAGTTGCTGCTCTGCAAAAACGATTAGATTAA

Upstream 100 bases:

>100_bases
GTCAAGCGTTTGGCATGCAAATTTCCTTAAACGTTCGTAAGCTTAAAACTTTACTTTAGATCAAACTGCTGTATAGTGAC
TTTATAGCTTCAATTTTGTA

Downstream 100 bases:

>100_bases
AACTTTTTTAAATTATTTCTTATAGGCTAGCTTTTTTGTGAACGTGATTATGTCGAAGACAGTTAAACTAATTTTGTGTT
TAATAATACCAATAATAGGA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNIPSIENCDLHNKAVLLRVDFNVPIKDGEIRDVTRILRALPTIQYLVNASAKIIIISHFGRPKARDNNLSLKNVIDTLS
QLLNKKVKFIDDCVGEKVQKAVSAMDAGDIILLENLRFYEGEKENDANFARQLASLADIYVNDAFSCSHRAHASISRITE
FLPSYAGFCLQDELKYLEKAVSFKAKPITAIVGGAKISTKIKVLMKLTEKVDYLVLGGAIANNFLSFSKVNIGKSFFQNG
VDDLLHNIVETANKNNCKIVVPEDVLVAVNSDYSTSISRKTESILDGDIILDIGSQTLSTISSIIASSKTLLWNGPIGVF
EHSAFASGTIGVMKIVSDLTHKGKLTSIIGGGDSLSAISAAGLTDKDFTYVSTGGGAFLDWLSGDEMPGVAALQKRLD

Sequences:

>Translated_398_residues
MNIPSIENCDLHNKAVLLRVDFNVPIKDGEIRDVTRILRALPTIQYLVNASAKIIIISHFGRPKARDNNLSLKNVIDTLS
QLLNKKVKFIDDCVGEKVQKAVSAMDAGDIILLENLRFYEGEKENDANFARQLASLADIYVNDAFSCSHRAHASISRITE
FLPSYAGFCLQDELKYLEKAVSFKAKPITAIVGGAKISTKIKVLMKLTEKVDYLVLGGAIANNFLSFSKVNIGKSFFQNG
VDDLLHNIVETANKNNCKIVVPEDVLVAVNSDYSTSISRKTESILDGDIILDIGSQTLSTISSIIASSKTLLWNGPIGVF
EHSAFASGTIGVMKIVSDLTHKGKLTSIIGGGDSLSAISAAGLTDKDFTYVSTGGGAFLDWLSGDEMPGVAALQKRLD
>Mature_398_residues
MNIPSIENCDLHNKAVLLRVDFNVPIKDGEIRDVTRILRALPTIQYLVNASAKIIIISHFGRPKARDNNLSLKNVIDTLS
QLLNKKVKFIDDCVGEKVQKAVSAMDAGDIILLENLRFYEGEKENDANFARQLASLADIYVNDAFSCSHRAHASISRITE
FLPSYAGFCLQDELKYLEKAVSFKAKPITAIVGGAKISTKIKVLMKLTEKVDYLVLGGAIANNFLSFSKVNIGKSFFQNG
VDDLLHNIVETANKNNCKIVVPEDVLVAVNSDYSTSISRKTESILDGDIILDIGSQTLSTISSIIASSKTLLWNGPIGVF
EHSAFASGTIGVMKIVSDLTHKGKLTSIIGGGDSLSAISAAGLTDKDFTYVSTGGGAFLDWLSGDEMPGVAALQKRLD

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=411, Percent_Identity=37.956204379562, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=36.7396593673966, Blast_Score=258, Evalue=7e-69,
Organism=Escherichia coli, GI1789294, Length=401, Percent_Identity=38.1546134663342, Blast_Score=265, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI17508823, Length=413, Percent_Identity=39.7094430992736, Blast_Score=279, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6319857, Length=412, Percent_Identity=39.3203883495146, Blast_Score=273, Evalue=2e-74,
Organism=Drosophila melanogaster, GI17136394, Length=409, Percent_Identity=40.8312958435208, Blast_Score=266, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24581203, Length=410, Percent_Identity=38.2926829268293, Blast_Score=258, Evalue=4e-69,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 43118; Mature: 43118

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIPSIENCDLHNKAVLLRVDFNVPIKDGEIRDVTRILRALPTIQYLVNASAKIIIISHF
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
GRPKARDNNLSLKNVIDTLSQLLNKKVKFIDDCVGEKVQKAVSAMDAGDIILLENLRFYE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHCC
GEKENDANFARQLASLADIYVNDAFSCSHRAHASISRITEFLPSYAGFCLQDELKYLEKA
CCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VSFKAKPITAIVGGAKISTKIKVLMKLTEKVDYLVLGGAIANNFLSFSKVNIGKSFFQNG
HCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHHCCHHHHHCC
VDDLLHNIVETANKNNCKIVVPEDVLVAVNSDYSTSISRKTESILDGDIILDIGSQTLST
HHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECHHHHHH
ISSIIASSKTLLWNGPIGVFEHSAFASGTIGVMKIVSDLTHKGKLTSIIGGGDSLSAISA
HHHHHHCCCEEEEECCCCHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHH
AGLTDKDFTYVSTGGGAFLDWLSGDEMPGVAALQKRLD
CCCCCCCCEEEECCCCCEECCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MNIPSIENCDLHNKAVLLRVDFNVPIKDGEIRDVTRILRALPTIQYLVNASAKIIIISHF
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
GRPKARDNNLSLKNVIDTLSQLLNKKVKFIDDCVGEKVQKAVSAMDAGDIILLENLRFYE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHCC
GEKENDANFARQLASLADIYVNDAFSCSHRAHASISRITEFLPSYAGFCLQDELKYLEKA
CCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VSFKAKPITAIVGGAKISTKIKVLMKLTEKVDYLVLGGAIANNFLSFSKVNIGKSFFQNG
HCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHHCCHHHHHCC
VDDLLHNIVETANKNNCKIVVPEDVLVAVNSDYSTSISRKTESILDGDIILDIGSQTLST
HHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECHHHHHH
ISSIIASSKTLLWNGPIGVFEHSAFASGTIGVMKIVSDLTHKGKLTSIIGGGDSLSAISA
HHHHHHCCCEEEEECCCCHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHH
AGLTDKDFTYVSTGGGAFLDWLSGDEMPGVAALQKRLD
CCCCCCCCEEEECCCCCEECCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA