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The map label for this gene is hyfB [C]

Identifier: 225630811

GI number: 225630811

Start: 1205246

End: 1206805

Strand: Reverse

Name: hyfB [C]

Synonym: WRi_011150

Alternate gene names: 225630811

Gene position: 1206805-1205246 (Counterclockwise)

Preceding gene: 225630812

Following gene: 225630810

Centisome position: 83.47

GC content: 33.21

Gene sequence:

>1560_bases
GTGCAACTTTATAAATCGTCACTGTTACTTCTACTGACTGTATTAATAGTTATAGTCCTAAAATTACCTGAAAATTTGAG
CATTGCAGTAAATTGGTCTCTCCCAATTCTAGATTTTGATTTATCTTTTCGTACCATACTAATATCTTTTTTATCAGTTG
CATTTTTAGCTGTTCTACCAGCACAAAATTTGACTTTTTCAGAGATGCTATCTTTTGCTGCTTACACTATTAGTTCACTT
TATGCAGTTTTATCTGAACATATGATATTGGTTATAATATTCTGTGAATTAATGACCATCAGTGCATTTTTTATTATCGC
AGCTAGCTGTAGAGATAGTGGACCTGCAATAAGGTACGCTTGTGTACACTTTTTCGTTGGAGTTATATTAATAGCAGGAT
TGGCACTTCAAAGCACTAACCTGATAATTTTGGGTTTATTGATAAACTGCGCTTGTTTTCCTTTTTCGTTTTGGGTTGTT
GATGCATATCCTGCTGCATCGCTATATGGCACTACATATCTCTCTTTATTTACCACTAAAGTCTCTTTTTTAGTGATGCT
TTTACACACTTATAACCTATGGCAAAATTGTGCTGAAATGTTAGCACTTGTAGGCACCATCACTGCAATTTACAGCATTA
TATTTGCTTCTCTCGAGCAAAATATTCGTAGATTTTTATGCTATAACGTTGTAGGACAAATGGGCTTGCTGATTATTGCA
GGAGGTTTACTCAGCCCTTCGGAAAAGGCAATACCAATTTTGATACTGCATATAATCTTCTCTCTTGTTTATCAATCATT
ATTGTTTGTGGTCAGCAATTCGATTATTTCACGAACAAAAACAATTAGTTTTAATGGAGTAGGCAAACTGATGTCAGTGG
AAGGCATGTGTGCTATAATCGCAATACTTACAATGGCTGCATTTCCTGGAACCGCTGGATTTATTAGCAAATCATACATT
ACAGCCGAGATTGAAGCAAGTGGTGCTAATTTAGAAGTGTATAAAAACTTATACAAGACTCTAAATTTGCTGCTTTATTT
AAGCGTGGGGCTCAAGTTTCTTTATTATTTATTTATTGCGAAAAGTAAGTCAAAACCTTTAGCAGAGGAGGGAGGAAAAA
TGACTATGATTATTTTAGCATTTATATGCGTTATTGCTGGCAACCCTTACTTACCTATTTACAATAAACCTTCGATTTTC
GATTTTGTGTACAACACAAAAAATATTTTGTCGCAATTTAATTTGTTATTATGTACCACTTTATTGTTCATTCTTTTACG
CAAGTTATTTTACCCGAGAATAAATTTTAAAATGGATGTTGATTGGATTTTTAGAGCTTTCATACCCTATATTATCTTGC
TGTTCAATCAATTGGTCTTTAAAGTGAGAGAAATGTCTGCCAACGTACTGCAAAACTTGACTAATTCACTTACTAGTTTA
TACTTTGATAATATTACTAAACTTAAGGAAGTGCTGGGTTATAACTCAGTGAGTTTTGTTTCAGCTTCTTCTCTCTTTTT
AATGAGTATTCTACTAATGCTATTATGTTTAAGTCGTTAA

Upstream 100 bases:

>100_bases
CTGATATAATTATATGATAATTAGTTTAATTAGTGGTTAATATGCAAATTGATTTTATTCATATATTGAGATAGCATAAA
CCATATTGGTTTTAATTATT

Downstream 100 bases:

>100_bases
CCGAAAGTTTAAATTCTGTATTTAGTAAACTAAGAGGAAAGTCAATCATTTCTGAAGATGATTTTAATCTTGCCATGCGT
GAAATACGCATAGCCTTAAT

Product: putative monovalent cation/H+ antiporter subunit D

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NADH/Ubiquinone/Plastoquinone; NADH Dehydrogenase; Oxidoreductase; Multisubunit Sodium/Proton Antiporter Mrpd Subunit; Formate Hydrogenlyase I Subunit D; NADH-Quinone Oxidoreductase Subunit N; NADH-Quinone Oxidoreductase Chain L; NADH-Ubiquinone/Plastoquinone; PH Adaptation Potassium Efflux System; Monovalent Cation/H+ Antiporter Subunit A; NADH-Ubiquinone Oxidoreductase Protein Chain M; Multisubunit Sodium/Proton Antiporter MrpD Subunit; NADH Dehydrogenase I Chain N; Na+/H+ Antiporter MnhA Component

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MQLYKSSLLLLLTVLIVIVLKLPENLSIAVNWSLPILDFDLSFRTILISFLSVAFLAVLPAQNLTFSEMLSFAAYTISSL
YAVLSEHMILVIIFCELMTISAFFIIAASCRDSGPAIRYACVHFFVGVILIAGLALQSTNLIILGLLINCACFPFSFWVV
DAYPAASLYGTTYLSLFTTKVSFLVMLLHTYNLWQNCAEMLALVGTITAIYSIIFASLEQNIRRFLCYNVVGQMGLLIIA
GGLLSPSEKAIPILILHIIFSLVYQSLLFVVSNSIISRTKTISFNGVGKLMSVEGMCAIIAILTMAAFPGTAGFISKSYI
TAEIEASGANLEVYKNLYKTLNLLLYLSVGLKFLYYLFIAKSKSKPLAEEGGKMTMIILAFICVIAGNPYLPIYNKPSIF
DFVYNTKNILSQFNLLLCTTLLFILLRKLFYPRINFKMDVDWIFRAFIPYIILLFNQLVFKVREMSANVLQNLTNSLTSL
YFDNITKLKEVLGYNSVSFVSASSLFLMSILLMLLCLSR

Sequences:

>Translated_519_residues
MQLYKSSLLLLLTVLIVIVLKLPENLSIAVNWSLPILDFDLSFRTILISFLSVAFLAVLPAQNLTFSEMLSFAAYTISSL
YAVLSEHMILVIIFCELMTISAFFIIAASCRDSGPAIRYACVHFFVGVILIAGLALQSTNLIILGLLINCACFPFSFWVV
DAYPAASLYGTTYLSLFTTKVSFLVMLLHTYNLWQNCAEMLALVGTITAIYSIIFASLEQNIRRFLCYNVVGQMGLLIIA
GGLLSPSEKAIPILILHIIFSLVYQSLLFVVSNSIISRTKTISFNGVGKLMSVEGMCAIIAILTMAAFPGTAGFISKSYI
TAEIEASGANLEVYKNLYKTLNLLLYLSVGLKFLYYLFIAKSKSKPLAEEGGKMTMIILAFICVIAGNPYLPIYNKPSIF
DFVYNTKNILSQFNLLLCTTLLFILLRKLFYPRINFKMDVDWIFRAFIPYIILLFNQLVFKVREMSANVLQNLTNSLTSL
YFDNITKLKEVLGYNSVSFVSASSLFLMSILLMLLCLSR
>Mature_519_residues
MQLYKSSLLLLLTVLIVIVLKLPENLSIAVNWSLPILDFDLSFRTILISFLSVAFLAVLPAQNLTFSEMLSFAAYTISSL
YAVLSEHMILVIIFCELMTISAFFIIAASCRDSGPAIRYACVHFFVGVILIAGLALQSTNLIILGLLINCACFPFSFWVV
DAYPAASLYGTTYLSLFTTKVSFLVMLLHTYNLWQNCAEMLALVGTITAIYSIIFASLEQNIRRFLCYNVVGQMGLLIIA
GGLLSPSEKAIPILILHIIFSLVYQSLLFVVSNSIISRTKTISFNGVGKLMSVEGMCAIIAILTMAAFPGTAGFISKSYI
TAEIEASGANLEVYKNLYKTLNLLLYLSVGLKFLYYLFIAKSKSKPLAEEGGKMTMIILAFICVIAGNPYLPIYNKPSIF
DFVYNTKNILSQFNLLLCTTLLFILLRKLFYPRINFKMDVDWIFRAFIPYIILLFNQLVFKVREMSANVLQNLTNSLTSL
YFDNITKLKEVLGYNSVSFVSASSLFLMSILLMLLCLSR

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788827, Length=292, Percent_Identity=24.3150684931507, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.-.-.- [C]

Molecular weight: Translated: 57931; Mature: 57931

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLYKSSLLLLLTVLIVIVLKLPENLSIAVNWSLPILDFDLSFRTILISFLSVAFLAVLP
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECHHHHHHHHHHHHHHHHHHHC
AQNLTFSEMLSFAAYTISSLYAVLSEHMILVIIFCELMTISAFFIIAASCRDSGPAIRYA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
CVHFFVGVILIAGLALQSTNLIILGLLINCACFPFSFWVVDAYPAASLYGTTYLSLFTTK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHH
VSFLVMLLHTYNLWQNCAEMLALVGTITAIYSIIFASLEQNIRRFLCYNVVGQMGLLIIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGLLSPSEKAIPILILHIIFSLVYQSLLFVVSNSIISRTKTISFNGVGKLMSVEGMCAII
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHH
AILTMAAFPGTAGFISKSYITAEIEASGANLEVYKNLYKTLNLLLYLSVGLKFLYYLFIA
HHHHHHHCCCCHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSKSKPLAEEGGKMTMIILAFICVIAGNPYLPIYNKPSIFDFVYNTKNILSQFNLLLCTT
CCCCCCCHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLFILLRKLFYPRINFKMDVDWIFRAFIPYIILLFNQLVFKVREMSANVLQNLTNSLTSL
HHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFDNITKLKEVLGYNSVSFVSASSLFLMSILLMLLCLSR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQLYKSSLLLLLTVLIVIVLKLPENLSIAVNWSLPILDFDLSFRTILISFLSVAFLAVLP
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECHHHHHHHHHHHHHHHHHHHC
AQNLTFSEMLSFAAYTISSLYAVLSEHMILVIIFCELMTISAFFIIAASCRDSGPAIRYA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
CVHFFVGVILIAGLALQSTNLIILGLLINCACFPFSFWVVDAYPAASLYGTTYLSLFTTK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHH
VSFLVMLLHTYNLWQNCAEMLALVGTITAIYSIIFASLEQNIRRFLCYNVVGQMGLLIIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGLLSPSEKAIPILILHIIFSLVYQSLLFVVSNSIISRTKTISFNGVGKLMSVEGMCAII
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHH
AILTMAAFPGTAGFISKSYITAEIEASGANLEVYKNLYKTLNLLLYLSVGLKFLYYLFIA
HHHHHHHCCCCHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSKSKPLAEEGGKMTMIILAFICVIAGNPYLPIYNKPSIFDFVYNTKNILSQFNLLLCTT
CCCCCCCHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLFILLRKLFYPRINFKMDVDWIFRAFIPYIILLFNQLVFKVREMSANVLQNLTNSLTSL
HHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFDNITKLKEVLGYNSVSFVSASSLFLMSILLMLLCLSR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA