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The map label for this gene is ispDF [H]

Identifier: 225630702

GI number: 225630702

Start: 1067111

End: 1068313

Strand: Direct

Name: ispDF [H]

Synonym: WRi_009950

Alternate gene names: 225630702

Gene position: 1067111-1068313 (Clockwise)

Preceding gene: 225630693

Following gene: 225630704

Centisome position: 73.8

GC content: 33.58

Gene sequence:

>1203_bases
ATGCACACTAACAGACTTAAGAAATACAAAATAGCAGCATTAATAGTTGCAGCAGGAGTAGGCAGTAGATGCAGTTCTAC
GATTCCTAAGCAGTATACAGAACTTGTAGGTAAATCTGTTTTATTTCATACAATTAAAAGATTTCTGACTAACCAATATA
TAGATCACATAAGAATAGCAATTAATAGAGACCATGAAAACTTCTATGAGAAAGCTATATCACTAATTACAGACACTAAA
TTACTAAGTCCAATATATGGAGGAGAAAACAGGCAAAGTTCAGTTAAACTAGGGCTTGAAAGCTTACAAAAAGTTAACCC
AGATTTTGTAGTTATACACGATGCTTGTAGGCCTTTTGTGTCAAATGTTCTGATAGATAATTTGGCTCAGTCCATGATTA
ATGATCAATATACAGGAGTAGTTCCAGCAATAGATGTTGAAGATACTATGTCATTGGTAAGTAATAATTTCATTGAATCT
ACAATTTCAAGGGAAAAACTTAGAGCCATACAAACTCCTCAAATTTTTAACTTTAAAGAACTATTATCATGTCACCAATC
AACCAAAGAATTTACTGATGATTCATCGCTAATGATAGAGCACAAAAAGCGCGTTGTGATTATTAAAGGTGAAAAAAGCA
ATTTTAAGTTAACCACAAAAGAAGATATCAATATGGCAAAACTCCTTTTTGAAGAGCCAAAATTTCGTGTCGGAACCGGT
TATGATATACATAAGTTCATTAAAGTTCAAAATGGTGCTGAAAGCTTTATAAAAATTTGCGGTGTGAAAATTGAACACAA
CATGGCAATAGAAGCGCACTCTGATGGTGATGTTGCAATACATGCAATCGTTGATGCAATACTCGGAGCACTGGGATATG
GCGACATAGGAGAGCACTTTCCTCCTAGTTCCTCTGAATGGAAAGATTGCAATTCATCTCACTTTCTTGACTTTGCTGCT
AAAAAAGCAAAAGAAAAAGGGTACAGCGTGTCTAATTTAGATATTACTATAGTTTGTGAAGAGCCTAAAATATCGCCTTA
CAAAGTAGAAATGAAGAAATTCATATCAAAAGCATTAGAAATTGATGATGAATTTGTAAATATCAAAGCAACCACTGCAG
AGAAATTAGGTTATATTGGAAGAAATGAAGGAATAGCAGTGCATGCTTCTGTGTTATTGCATACAAACTTTTATTGGAAA
TAG

Upstream 100 bases:

>100_bases
TGCATTCTAGCATCATATTATATCTTAATTAAAATAATACACTCTTTTTTTAAAACTGTAAATAACCTGTTACTATACAC
TTACGCTGAAAATGATAAAT

Downstream 100 bases:

>100_bases
AAACGAAAAAACTTACTTGACACCCTTCGCCAGCCCCCTTATCATGAAACTGAAGCTATTTATTTATCTTCTCTGTACAG
ATTAAATGACAAAAAAACTC

Product: ispD/ispF bifunctional enzyme

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MHTNRLKKYKIAALIVAAGVGSRCSSTIPKQYTELVGKSVLFHTIKRFLTNQYIDHIRIAINRDHENFYEKAISLITDTK
LLSPIYGGENRQSSVKLGLESLQKVNPDFVVIHDACRPFVSNVLIDNLAQSMINDQYTGVVPAIDVEDTMSLVSNNFIES
TISREKLRAIQTPQIFNFKELLSCHQSTKEFTDDSSLMIEHKKRVVIIKGEKSNFKLTTKEDINMAKLLFEEPKFRVGTG
YDIHKFIKVQNGAESFIKICGVKIEHNMAIEAHSDGDVAIHAIVDAILGALGYGDIGEHFPPSSSEWKDCNSSHFLDFAA
KKAKEKGYSVSNLDITIVCEEPKISPYKVEMKKFISKALEIDDEFVNIKATTAEKLGYIGRNEGIAVHASVLLHTNFYWK

Sequences:

>Translated_400_residues
MHTNRLKKYKIAALIVAAGVGSRCSSTIPKQYTELVGKSVLFHTIKRFLTNQYIDHIRIAINRDHENFYEKAISLITDTK
LLSPIYGGENRQSSVKLGLESLQKVNPDFVVIHDACRPFVSNVLIDNLAQSMINDQYTGVVPAIDVEDTMSLVSNNFIES
TISREKLRAIQTPQIFNFKELLSCHQSTKEFTDDSSLMIEHKKRVVIIKGEKSNFKLTTKEDINMAKLLFEEPKFRVGTG
YDIHKFIKVQNGAESFIKICGVKIEHNMAIEAHSDGDVAIHAIVDAILGALGYGDIGEHFPPSSSEWKDCNSSHFLDFAA
KKAKEKGYSVSNLDITIVCEEPKISPYKVEMKKFISKALEIDDEFVNIKATTAEKLGYIGRNEGIAVHASVLLHTNFYWK
>Mature_400_residues
MHTNRLKKYKIAALIVAAGVGSRCSSTIPKQYTELVGKSVLFHTIKRFLTNQYIDHIRIAINRDHENFYEKAISLITDTK
LLSPIYGGENRQSSVKLGLESLQKVNPDFVVIHDACRPFVSNVLIDNLAQSMINDQYTGVVPAIDVEDTMSLVSNNFIES
TISREKLRAIQTPQIFNFKELLSCHQSTKEFTDDSSLMIEHKKRVVIIKGEKSNFKLTTKEDINMAKLLFEEPKFRVGTG
YDIHKFIKVQNGAESFIKICGVKIEHNMAIEAHSDGDVAIHAIVDAILGALGYGDIGEHFPPSSSEWKDCNSSHFLDFAA
KKAKEKGYSVSNLDITIVCEEPKISPYKVEMKKFISKALEIDDEFVNIKATTAEKLGYIGRNEGIAVHASVLLHTNFYWK

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=235, Percent_Identity=22.9787234042553, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789103, Length=159, Percent_Identity=41.5094339622642, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789104, Length=225, Percent_Identity=27.1111111111111, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 44977; Mature: 44977

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTNRLKKYKIAALIVAAGVGSRCSSTIPKQYTELVGKSVLFHTIKRFLTNQYIDHIRIA
CCCCHHHHHHHHHHEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
INRDHENFYEKAISLITDTKLLSPIYGGENRQSSVKLGLESLQKVNPDFVVIHDACRPFV
EECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHH
SNVLIDNLAQSMINDQYTGVVPAIDVEDTMSLVSNNFIESTISREKLRAIQTPQIFNFKE
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LLSCHQSTKEFTDDSSLMIEHKKRVVIIKGEKSNFKLTTKEDINMAKLLFEEPKFRVGTG
HHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCEEEECCCCCHHHHHHHHCCCEEECCC
YDIHKFIKVQNGAESFIKICGVKIEHNMAIEAHSDGDVAIHAIVDAILGALGYGDIGEHF
CCHHHHEEECCCHHHHHHHHCEEEECCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCC
PPSSSEWKDCNSSHFLDFAAKKAKEKGYSVSNLDITIVCEEPKISPYKVEMKKFISKALE
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHC
IDDEFVNIKATTAEKLGYIGRNEGIAVHASVLLHTNFYWK
CCCCEEEEEEECHHHHCCCCCCCCEEEEEEEEEECCCCCC
>Mature Secondary Structure
MHTNRLKKYKIAALIVAAGVGSRCSSTIPKQYTELVGKSVLFHTIKRFLTNQYIDHIRIA
CCCCHHHHHHHHHHEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
INRDHENFYEKAISLITDTKLLSPIYGGENRQSSVKLGLESLQKVNPDFVVIHDACRPFV
EECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHH
SNVLIDNLAQSMINDQYTGVVPAIDVEDTMSLVSNNFIESTISREKLRAIQTPQIFNFKE
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LLSCHQSTKEFTDDSSLMIEHKKRVVIIKGEKSNFKLTTKEDINMAKLLFEEPKFRVGTG
HHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCEEEECCCCCHHHHHHHHCCCEEECCC
YDIHKFIKVQNGAESFIKICGVKIEHNMAIEAHSDGDVAIHAIVDAILGALGYGDIGEHF
CCHHHHEEECCCHHHHHHHHCEEEECCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCC
PPSSSEWKDCNSSHFLDFAAKKAKEKGYSVSNLDITIVCEEPKISPYKVEMKKFISKALE
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHC
IDDEFVNIKATTAEKLGYIGRNEGIAVHASVLLHTNFYWK
CCCCEEEEEEECHHHHCCCCCCCCEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA