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The map label for this gene is pstA [H]

Identifier: 225630683

GI number: 225630683

Start: 1049871

End: 1051121

Strand: Reverse

Name: pstA [H]

Synonym: WRi_009760

Alternate gene names: 225630683

Gene position: 1051121-1049871 (Counterclockwise)

Preceding gene: 225630684

Following gene: 225630682

Centisome position: 72.7

GC content: 34.61

Gene sequence:

>1251_bases
ATGAGTATAAAGAAAAAATTCCTTAAATTACTTCAGTCAAGGCGTGTACATGCTCGTATAAAAAGGAAAAATAAAAAGAA
CAGAGCGCTACGCTTTTGTTCTTTCACGGCACTAGTTATCTCACTTGGCTGTCCTGTGTGTATATTGTTGAGTATATTAG
TTAACTCTTACAGTGCCTTAACAGTAACGAAAATTTTATTGCCAGTTGAAATAAATGCTGATCTTGCTTTGGCGAGTAAT
CCTAGTGACTTGCGGTATAAGTCCATTGGCTTACTCAACGATTCTTTACGTAAGGTGTTTGGAGGAACTGATTTTAAAGA
CGGTGACGAAATTTTAAGTCGTAATTCTTACAAAGAACTAGAGAAATTTTTTCGTAAGAAAGTAAAAGATAGTGGTGAAT
ATGAGATCTGGTTTACCGCGTCAAGTATAATTAATTCAATAAATAAAGATAAATATTTGAATGATCGTTATGCTAAATTA
CTTGATTGGCTAAAAGAAAAGAGGAGAGTGAAAAAATTTTTTAATAAGTCTTTATTCCTTAAATCTGACTCTCGTGAACC
TGAAAATGCAGGGATTTTAGGAGCGTTTATCGGTTCATTAATGACAATTATAGTGTGCCTAGCATTAGCATTACCAATAG
GAATTATGTCGGGCATCTGTCTTTACGAATTTATGCCTAAAAATAGGCTAATGACTAATATTGTAGAAATTAGCATAAAT
AATCTTGCTGCAGTGCCTTCAATAATATTTGGTGTAGTGGGCTTAACTCTCTATCTTGGCATATTTGGGCTACCACGCTC
TTCACCACTTGTTGGTGGAATGACTCTTTCATTTATGATGTTACCTAATATTATAATTGCAACAAAAAATGCCTTTGCGA
ATGTTCCTATTACGATAAAAGATGCAGCGTTTGCGCTCGGTGCACCTCACATCAAGGTGATATTAGATCACTCTTTACCG
ATTGCGTTACCAAGAATAATACATGGTACTGTGCTTGCAATTGCAAGGATTTTAGGTGAATCTTCTCCTTTACTTATGAT
GGGTATGGTGGCATTTATTGCTGATACACCTACGTCCTTTTTCGATCCGGCGACTGTTCTACCTGTGCAAATATACATAT
GGTCAAGCAATCCTGAAATTGCATTTGTTGAACTTGCTGCTATCGCAATTATAGCTTTATTATTGGTGTTATTTGCGTTA
AATTTAGTAGCAAATTTTGTAAAAAGGAAATTCGAATTTTTTAATTTTTAG

Upstream 100 bases:

>100_bases
AAAAATTATTTGCAGGCAAAGCCTGCTGGATCCCAGTGTCAGCTACTTGCATGACAAAAAAGGGGAAAGTTTTTTCAAAT
CCTCGTGTAAAGAAAAGTAA

Downstream 100 bases:

>100_bases
CAATTCATGAAAATCACAGTCCTATCTGGTTATGGCTTAAATTGCGAAAAAGAAACTGCATTTGCATTTATGGAATGCAG
CAGAAAACTTGGTATTGGTA

Product: phosphate ABC transporter, permease protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSIKKKFLKLLQSRRVHARIKRKNKKNRALRFCSFTALVISLGCPVCILLSILVNSYSALTVTKILLPVEINADLALASN
PSDLRYKSIGLLNDSLRKVFGGTDFKDGDEILSRNSYKELEKFFRKKVKDSGEYEIWFTASSIINSINKDKYLNDRYAKL
LDWLKEKRRVKKFFNKSLFLKSDSREPENAGILGAFIGSLMTIIVCLALALPIGIMSGICLYEFMPKNRLMTNIVEISIN
NLAAVPSIIFGVVGLTLYLGIFGLPRSSPLVGGMTLSFMMLPNIIIATKNAFANVPITIKDAAFALGAPHIKVILDHSLP
IALPRIIHGTVLAIARILGESSPLLMMGMVAFIADTPTSFFDPATVLPVQIYIWSSNPEIAFVELAAIAIIALLLVLFAL
NLVANFVKRKFEFFNF

Sequences:

>Translated_416_residues
MSIKKKFLKLLQSRRVHARIKRKNKKNRALRFCSFTALVISLGCPVCILLSILVNSYSALTVTKILLPVEINADLALASN
PSDLRYKSIGLLNDSLRKVFGGTDFKDGDEILSRNSYKELEKFFRKKVKDSGEYEIWFTASSIINSINKDKYLNDRYAKL
LDWLKEKRRVKKFFNKSLFLKSDSREPENAGILGAFIGSLMTIIVCLALALPIGIMSGICLYEFMPKNRLMTNIVEISIN
NLAAVPSIIFGVVGLTLYLGIFGLPRSSPLVGGMTLSFMMLPNIIIATKNAFANVPITIKDAAFALGAPHIKVILDHSLP
IALPRIIHGTVLAIARILGESSPLLMMGMVAFIADTPTSFFDPATVLPVQIYIWSSNPEIAFVELAAIAIIALLLVLFAL
NLVANFVKRKFEFFNF
>Mature_415_residues
SIKKKFLKLLQSRRVHARIKRKNKKNRALRFCSFTALVISLGCPVCILLSILVNSYSALTVTKILLPVEINADLALASNP
SDLRYKSIGLLNDSLRKVFGGTDFKDGDEILSRNSYKELEKFFRKKVKDSGEYEIWFTASSIINSINKDKYLNDRYAKLL
DWLKEKRRVKKFFNKSLFLKSDSREPENAGILGAFIGSLMTIIVCLALALPIGIMSGICLYEFMPKNRLMTNIVEISINN
LAAVPSIIFGVVGLTLYLGIFGLPRSSPLVGGMTLSFMMLPNIIIATKNAFANVPITIKDAAFALGAPHIKVILDHSLPI
ALPRIIHGTVLAIARILGESSPLLMMGMVAFIADTPTSFFDPATVLPVQIYIWSSNPEIAFVELAAIAIIALLLVLFALN
LVANFVKRKFEFFNF

Specific function: Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0581

COG function: function code P; ABC-type phosphate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790163, Length=254, Percent_Identity=30.3149606299213, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1790164, Length=197, Percent_Identity=25.3807106598985, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005672 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 46229; Mature: 46098

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKKKFLKLLQSRRVHARIKRKNKKNRALRFCSFTALVISLGCPVCILLSILVNSYSAL
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHH
TVTKILLPVEINADLALASNPSDLRYKSIGLLNDSLRKVFGGTDFKDGDEILSRNSYKEL
HHHHHEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHH
EKFFRKKVKDSGEYEIWFTASSIINSINKDKYLNDRYAKLLDWLKEKRRVKKFFNKSLFL
HHHHHHHCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
KSDSREPENAGILGAFIGSLMTIIVCLALALPIGIMSGICLYEFMPKNRLMTNIVEISIN
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
NLAAVPSIIFGVVGLTLYLGIFGLPRSSPLVGGMTLSFMMLPNIIIATKNAFANVPITIK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCEEEE
DAAFALGAPHIKVILDHSLPIALPRIIHGTVLAIARILGESSPLLMMGMVAFIADTPTSF
CHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
FDPATVLPVQIYIWSSNPEIAFVELAAIAIIALLLVLFALNLVANFVKRKFEFFNF
CCCCEEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIKKKFLKLLQSRRVHARIKRKNKKNRALRFCSFTALVISLGCPVCILLSILVNSYSAL
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHH
TVTKILLPVEINADLALASNPSDLRYKSIGLLNDSLRKVFGGTDFKDGDEILSRNSYKEL
HHHHHEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHH
EKFFRKKVKDSGEYEIWFTASSIINSINKDKYLNDRYAKLLDWLKEKRRVKKFFNKSLFL
HHHHHHHCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
KSDSREPENAGILGAFIGSLMTIIVCLALALPIGIMSGICLYEFMPKNRLMTNIVEISIN
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
NLAAVPSIIFGVVGLTLYLGIFGLPRSSPLVGGMTLSFMMLPNIIIATKNAFANVPITIK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCEEEE
DAAFALGAPHIKVILDHSLPIALPRIIHGTVLAIARILGESSPLLMMGMVAFIADTPTSF
CHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
FDPATVLPVQIYIWSSNPEIAFVELAAIAIIALLLVLFALNLVANFVKRKFEFFNF
CCCCEEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2993631; 3881386; 7686882; 9278503; 1447208 [H]