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The map label for this gene is murE [H]

Identifier: 225630606

GI number: 225630606

Start: 951481

End: 952908

Strand: Reverse

Name: murE [H]

Synonym: WRi_008770

Alternate gene names: 225630606

Gene position: 952908-951481 (Counterclockwise)

Preceding gene: 225630607

Following gene: 225630600

Centisome position: 65.91

GC content: 36.48

Gene sequence:

>1428_bases
ATGAAACTGAGAGAATTACTGCATAACATTATTGATGTTAACTTTGATATTGAAATCAAAGGCGTCACATGTAATCCCAA
GAAAATCAAGGAAGGTTATCTTTTTGTTTGTGTGTCAGAAAGGGATAGGATTTACATAGATCAGATATTATCTTGTGGAG
CTAAAGCGATAATTGCAAGTGATTGCATAACAGAAGCTTTAAGCCTTGGTATCATTCCAACGCGTGATGCTGGAATCTGT
ATCTTCCACCCAAACCCTCAAGAAATATACAGCGAAATAGTCAGCAGGTTTTATCAATTCAAACAGCCAAAATATGTTGC
TGCTGTAACAGGCACAAATGGCAAGACTTCAGTAGTAGAATTTTGCCGCCAGATCTGGCAAAACTCTGGCTATAATGCTG
CGTCTATTGGAACACTTGGAACATGCATCAATAATGGCAGAAAAGGTAATAGCAACAGCCTTACCACTCCAGGTGCAGAA
GACCTTTACGCAACATTGCGTGGTATAAGTGTAGAACACTTAGTGTTGGAATCATCAAGTCATGGGATTGATCAATACAG
AATCCATGGATTAAAGTTGAGTGCTGCAGCTTTTACTAATTTCTCGCAAGATCATTTAGATTACCATAAAAGTCTTGGTG
GATATTTAGAAACTAAAAAAAGGTTATTTTACGAAGTATTACCAGAAGGAAAAACAGCGATTTTAAATGCGGATATAGAT
GAATACGGCGCATTGCTTAAAATAGCTGAAAAACGCAGTAATAAAGTTGTGACTTATGGAAAAAAAGGCTCTGATATTAC
TTTATTAGAGCAAACATCAACCCCTAATGGTCAACATCTTACAGTAAAAATTGATAACCAAATTTACAATACATTTTTCC
CAGTTTTAGGAAAATTTCAAGCATATAATCTGCTATGTGCAATTGGTATATCTGGATTAAATTACAGAGAAATATGCGTA
GAAAAACTCGTTTCTCCACCAGGAAGAATGGAAAAAGTGAAACCTTTTGCATTCGTGGATTACGCTCATACTCCAAGTGC
GCTTAAACAAGCCCTATTGTCTTTAAAATGGCATATCAACAAAAAAATAATTCTAGTTTTTGGATGCGGTGGAAATCGCG
ATCAGACGAAACGTTCAGAAATGGGCAAAATAGCACAAATGTATGCAGATAAAGTAATAATTACAGATGATAATTCGCGT
GATGAAGATCCGGCAAAAATTCGCCACGATATTTTGCTACATTGCCCTGATGCACTGGAGATAGGAGGTAGGAGAGAAGC
TATAGAGAAAGGCGTAGATATTGCCTATAACGAGGGTATGATTCTGCTAGTTGCAGGAAAGGGAAATGAGAAATTTCAAA
TTATAGGCAACCAAACTTTTGAATTTAGCGATGTTGAGGTTATTAAAAATCATGCCTTAACATACTGA

Upstream 100 bases:

>100_bases
ATATTTTGTGGACTGGAAGATCCTGAAGATTTAATCTTTGACCTTAATGACGCACTAAAAAGGTTACCATGTTTAGACTT
TCAAAACGATGAAGTAGGAC

Downstream 100 bases:

>100_bases
TTTTCGCAATGGGCTTACCTCCGCTAATGTGCGAATATTGAAGCAAAAGTGATGTCATCCCAGTGCTTGACACTGGGATG
ACACCCTCCTTGGCAAACAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate-2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKLRELLHNIIDVNFDIEIKGVTCNPKKIKEGYLFVCVSERDRIYIDQILSCGAKAIIASDCITEALSLGIIPTRDAGIC
IFHPNPQEIYSEIVSRFYQFKQPKYVAAVTGTNGKTSVVEFCRQIWQNSGYNAASIGTLGTCINNGRKGNSNSLTTPGAE
DLYATLRGISVEHLVLESSSHGIDQYRIHGLKLSAAAFTNFSQDHLDYHKSLGGYLETKKRLFYEVLPEGKTAILNADID
EYGALLKIAEKRSNKVVTYGKKGSDITLLEQTSTPNGQHLTVKIDNQIYNTFFPVLGKFQAYNLLCAIGISGLNYREICV
EKLVSPPGRMEKVKPFAFVDYAHTPSALKQALLSLKWHINKKIILVFGCGGNRDQTKRSEMGKIAQMYADKVIITDDNSR
DEDPAKIRHDILLHCPDALEIGGRREAIEKGVDIAYNEGMILLVAGKGNEKFQIIGNQTFEFSDVEVIKNHALTY

Sequences:

>Translated_475_residues
MKLRELLHNIIDVNFDIEIKGVTCNPKKIKEGYLFVCVSERDRIYIDQILSCGAKAIIASDCITEALSLGIIPTRDAGIC
IFHPNPQEIYSEIVSRFYQFKQPKYVAAVTGTNGKTSVVEFCRQIWQNSGYNAASIGTLGTCINNGRKGNSNSLTTPGAE
DLYATLRGISVEHLVLESSSHGIDQYRIHGLKLSAAAFTNFSQDHLDYHKSLGGYLETKKRLFYEVLPEGKTAILNADID
EYGALLKIAEKRSNKVVTYGKKGSDITLLEQTSTPNGQHLTVKIDNQIYNTFFPVLGKFQAYNLLCAIGISGLNYREICV
EKLVSPPGRMEKVKPFAFVDYAHTPSALKQALLSLKWHINKKIILVFGCGGNRDQTKRSEMGKIAQMYADKVIITDDNSR
DEDPAKIRHDILLHCPDALEIGGRREAIEKGVDIAYNEGMILLVAGKGNEKFQIIGNQTFEFSDVEVIKNHALTY
>Mature_475_residues
MKLRELLHNIIDVNFDIEIKGVTCNPKKIKEGYLFVCVSERDRIYIDQILSCGAKAIIASDCITEALSLGIIPTRDAGIC
IFHPNPQEIYSEIVSRFYQFKQPKYVAAVTGTNGKTSVVEFCRQIWQNSGYNAASIGTLGTCINNGRKGNSNSLTTPGAE
DLYATLRGISVEHLVLESSSHGIDQYRIHGLKLSAAAFTNFSQDHLDYHKSLGGYLETKKRLFYEVLPEGKTAILNADID
EYGALLKIAEKRSNKVVTYGKKGSDITLLEQTSTPNGQHLTVKIDNQIYNTFFPVLGKFQAYNLLCAIGISGLNYREICV
EKLVSPPGRMEKVKPFAFVDYAHTPSALKQALLSLKWHINKKIILVFGCGGNRDQTKRSEMGKIAQMYADKVIITDDNSR
DEDPAKIRHDILLHCPDALEIGGRREAIEKGVDIAYNEGMILLVAGKGNEKFQIIGNQTFEFSDVEVIKNHALTY

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=500, Percent_Identity=33.4, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 52867; Mature: 52867

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRELLHNIIDVNFDIEIKGVTCNPKKIKEGYLFVCVSERDRIYIDQILSCGAKAIIAS
CCHHHHHHHHHCCCEEEEEEEEECCHHHCCCCEEEEEECCCCCCHHHHHHHCCCHHHHHH
DCITEALSLGIIPTRDAGICIFHPNPQEIYSEIVSRFYQFKQPKYVAAVTGTNGKTSVVE
HHHHHHHHCCCEECCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHH
FCRQIWQNSGYNAASIGTLGTCINNGRKGNSNSLTTPGAEDLYATLRGISVEHLVLESSS
HHHHHHHCCCCCEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCC
HGIDQYRIHGLKLSAAAFTNFSQDHLDYHKSLGGYLETKKRLFYEVLPEGKTAILNADID
CCCCEEEEEEEEEEHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEECCHH
EYGALLKIAEKRSNKVVTYGKKGSDITLLEQTSTPNGQHLTVKIDNQIYNTFFPVLGKFQ
HHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHCCHH
AYNLLCAIGISGLNYREICVEKLVSPPGRMEKVKPFAFVDYAHTPSALKQALLSLKWHIN
HHEEEEEECCCCCCHHHHHHHHHCCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHEECC
KKIILVFGCGGNRDQTKRSEMGKIAQMYADKVIITDDNSRDEDPAKIRHDILLHCPDALE
CEEEEEEECCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHEEEECCCHHH
IGGRREAIEKGVDIAYNEGMILLVAGKGNEKFQIIGNQTFEFSDVEVIKNHALTY
CCCCHHHHHCCCCEEECCCEEEEEECCCCCEEEEECCCEEECCHHHHHHHCCCCC
>Mature Secondary Structure
MKLRELLHNIIDVNFDIEIKGVTCNPKKIKEGYLFVCVSERDRIYIDQILSCGAKAIIAS
CCHHHHHHHHHCCCEEEEEEEEECCHHHCCCCEEEEEECCCCCCHHHHHHHCCCHHHHHH
DCITEALSLGIIPTRDAGICIFHPNPQEIYSEIVSRFYQFKQPKYVAAVTGTNGKTSVVE
HHHHHHHHCCCEECCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHH
FCRQIWQNSGYNAASIGTLGTCINNGRKGNSNSLTTPGAEDLYATLRGISVEHLVLESSS
HHHHHHHCCCCCEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCC
HGIDQYRIHGLKLSAAAFTNFSQDHLDYHKSLGGYLETKKRLFYEVLPEGKTAILNADID
CCCCEEEEEEEEEEHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEECCHH
EYGALLKIAEKRSNKVVTYGKKGSDITLLEQTSTPNGQHLTVKIDNQIYNTFFPVLGKFQ
HHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHCCHH
AYNLLCAIGISGLNYREICVEKLVSPPGRMEKVKPFAFVDYAHTPSALKQALLSLKWHIN
HHEEEEEECCCCCCHHHHHHHHHCCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHEECC
KKIILVFGCGGNRDQTKRSEMGKIAQMYADKVIITDDNSRDEDPAKIRHDILLHCPDALE
CEEEEEEECCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHEEEECCCHHH
IGGRREAIEKGVDIAYNEGMILLVAGKGNEKFQIIGNQTFEFSDVEVIKNHALTY
CCCCHHHHHCCCCEEECCCEEEEEECCCCCEEEEECCCEEECCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA