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The map label for this gene is pnp [H]

Identifier: 225630576

GI number: 225630576

Start: 911617

End: 913890

Strand: Reverse

Name: pnp [H]

Synonym: WRi_008390

Alternate gene names: 225630576

Gene position: 913890-911617 (Counterclockwise)

Preceding gene: 225630577

Following gene: 225630572

Centisome position: 63.21

GC content: 36.9

Gene sequence:

>2274_bases
ATGTTTAAAATTATAAAAAAATCTATAGAGTGGGGTGGTCGTACCTTATCTTTAGAAACAGGAAAAATAGCACGCCAAGC
ACATGGTTCAGTAGTTGTAAATTACGGTGATACTTCTGTTTTAGTAACTGTTGTAAGTAAAAAGAAGGAAGAAAATGTTG
ATTTTCTTCCTTTAAATGTACAGTTTATCGCAAAAAGCTATGCCATGGGTAAGATCCCTGGCGGCTTTTTTAAAAGAGAA
GGCAAGCCATCTGATAGAGAAACTTTAATCTCAAGGGTAATAGATAGAAGTATAAGACCACTATTCCCAGAAGGATTTCA
TGATGAAATTAGTGTGGTGTGCAATCTATTAACTTATGATACAGTCAATCCTCCTGAAGTGCCAGCATTGATTGGTGCTG
TTGCAGCTCTTGCAATTTCTGGTGTTCCCTTTCACTTTACTATAGCTGGAGTGATGGTTGGTTGTGATGAAAATAATAAC
TATATACTCAACCCTTCTGTTCAAGAGATGAAAGCAAGCAGCTTGGATCTGTTTTTGTCTGGTGATGAAAATTCAATTTT
AATGGTTGAATCAGAAGTGAAAGAGCTCTCTGAAGAAAATGTTTTTAATGCAATAAAATTTGGCCATGAACACCTTAAGC
CTGTCATTAAGCTCATAAAAGAGTTTGCTGATACAATTGGCAATAAACCTGAGAGCTTTGCCCCTATTGATGCATCAGAT
ATAACACAAGAGCTCGAAAAATACGGTAAAGATTTTGAAAAAGCATATTCGCAAACAGTAAAACAAGAGCGAGTTCAAGC
TCTAGAAGCGATCAGAGAGAATATATTGAATACTCTTAAAGAAACTGGAAAAGACGAAAAGTTAATTACGTATGCAGTAA
AAAACTTTGAAAGATCTTTAGTACGTGAAATAATTAGGAAGAAAAGTGTAAGGATAGACGGTCGTAAGCATGATGAGATA
CGTCAGATAGAAGTTGAAGTTGATATTCTGTCCAAAACTCACGGTTCTGCACTATTTACAAGAGGCAATACTCAGGCACT
GGTTGTTACTGCTCTTGGCACCACTCAAGATGAGCAAATTGTGGATGATATTGAAGGGGATAGACGTGAGCATTTCATGT
TGCATTATAACTTTCCCTCATTTGCTGTTGGAGAAACTTCTGCTGCGCGTGCACCAGGAAGAAGAGAAATCGGTCATGGC
AAACTTGCTTGGAAAGCAATTCATCCTGTTTTACCCGATAAGTCTGAATTTCCTTATACAATAAGAGTAGTGTCCGAAAT
TTTGGAGTCTGATGGTTCTTCTTCTATGGCAACAGTTTGTGGGACTTCTCTTGCTTTAATGGATACGGGTGTGCCAATAA
AGGCCCCTGTTGCTGGAATTGCTATGGGTCTCATCAAAGATAAAGACGAGTATGTAATACTTTCCGATATACTTGGTGAT
GAAGATTATCTTGGTGATATGGACTTTAAAGTAGCAGGAACTAGTGAAGGGGTTACGGCGCTACAAATGGACATGAAAAT
TTCTGGTATAAGCTTTGAAATTGTTGAAAAATCTTTAGAACAGGCAAAAGCTGGAAGATTACATATTTTAGAAAAAATGA
ATGCAGTGATTTCAGAACACAGTGATGATGTCAAAGACCATGCACCAAGGATGTTATCATTTTACATAGATAAAGACAAA
ATTTCTGCTGCTATAGGTTCTAAGGGGAAAAATATACGCAGTGTGTGTGAAAGAAGTAATGCAAAAATTGAAATAGGAGA
TGATGGTAAAGTTTCTGTTTTTGCTACGAGTGGCACTGAAGCTGAAATTGCAAAGAGTATGATGATTGATTCAATAACAG
AACTAGAACAAGGTTCTATAGTTGATGTCAAGGTTGTAAGAATAGAGAAGTCTATTGTAGAGCTTGAATTTCTTAATGGC
AGAAAAGGAAAAATGCACATAAGTGAAGTAGCTAATGAACATATAGATTCTATAGAGAGCGTACTTAAACAAGATGATAC
TTTCAAAGCACTAGTAATTGACTTCGAAAAAGGTGGGTGTCCAAAGTTGTCAAGACGTCGGGTTGATCAAGAGACGGGAG
AGTTTTTTGAAGGTGAGCTTTACAACGAGGAAAGGAAAGATGGTCCAAATGACAGGGATAATTATTACAATAATTCATTT
AGTAGAAAACCTGGAGGAAGTCACCATAAGAGGCCTCCTCGTCCTCGTTCTGGTTTCAGCAACAGAAATAGGCCGAAATT
TGGTAATAATGATTCATCATCAGGTTTTTATTAA

Upstream 100 bases:

>100_bases
GACGCAAGCACTTAAATTATATAAAGCGTAAATTTGGTAATGAAGCCTATCAGGAATTAATAGAGAAGTTAGGCATTAGA
AAATAATCGAGGAATTTAGT

Downstream 100 bases:

>100_bases
GAGTAAACTTCTTGCGTTACCTCTCGTCATTCCAGTGCTTAACACTGGAATGACACCCTTTCTGGTGAAGGTTGTTCTCA
AATCACAATGTTAGTACAAT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 757; Mature: 757

Protein sequence:

>757_residues
MFKIIKKSIEWGGRTLSLETGKIARQAHGSVVVNYGDTSVLVTVVSKKKEENVDFLPLNVQFIAKSYAMGKIPGGFFKRE
GKPSDRETLISRVIDRSIRPLFPEGFHDEISVVCNLLTYDTVNPPEVPALIGAVAALAISGVPFHFTIAGVMVGCDENNN
YILNPSVQEMKASSLDLFLSGDENSILMVESEVKELSEENVFNAIKFGHEHLKPVIKLIKEFADTIGNKPESFAPIDASD
ITQELEKYGKDFEKAYSQTVKQERVQALEAIRENILNTLKETGKDEKLITYAVKNFERSLVREIIRKKSVRIDGRKHDEI
RQIEVEVDILSKTHGSALFTRGNTQALVVTALGTTQDEQIVDDIEGDRREHFMLHYNFPSFAVGETSAARAPGRREIGHG
KLAWKAIHPVLPDKSEFPYTIRVVSEILESDGSSSMATVCGTSLALMDTGVPIKAPVAGIAMGLIKDKDEYVILSDILGD
EDYLGDMDFKVAGTSEGVTALQMDMKISGISFEIVEKSLEQAKAGRLHILEKMNAVISEHSDDVKDHAPRMLSFYIDKDK
ISAAIGSKGKNIRSVCERSNAKIEIGDDGKVSVFATSGTEAEIAKSMMIDSITELEQGSIVDVKVVRIEKSIVELEFLNG
RKGKMHISEVANEHIDSIESVLKQDDTFKALVIDFEKGGCPKLSRRRVDQETGEFFEGELYNEERKDGPNDRDNYYNNSF
SRKPGGSHHKRPPRPRSGFSNRNRPKFGNNDSSSGFY

Sequences:

>Translated_757_residues
MFKIIKKSIEWGGRTLSLETGKIARQAHGSVVVNYGDTSVLVTVVSKKKEENVDFLPLNVQFIAKSYAMGKIPGGFFKRE
GKPSDRETLISRVIDRSIRPLFPEGFHDEISVVCNLLTYDTVNPPEVPALIGAVAALAISGVPFHFTIAGVMVGCDENNN
YILNPSVQEMKASSLDLFLSGDENSILMVESEVKELSEENVFNAIKFGHEHLKPVIKLIKEFADTIGNKPESFAPIDASD
ITQELEKYGKDFEKAYSQTVKQERVQALEAIRENILNTLKETGKDEKLITYAVKNFERSLVREIIRKKSVRIDGRKHDEI
RQIEVEVDILSKTHGSALFTRGNTQALVVTALGTTQDEQIVDDIEGDRREHFMLHYNFPSFAVGETSAARAPGRREIGHG
KLAWKAIHPVLPDKSEFPYTIRVVSEILESDGSSSMATVCGTSLALMDTGVPIKAPVAGIAMGLIKDKDEYVILSDILGD
EDYLGDMDFKVAGTSEGVTALQMDMKISGISFEIVEKSLEQAKAGRLHILEKMNAVISEHSDDVKDHAPRMLSFYIDKDK
ISAAIGSKGKNIRSVCERSNAKIEIGDDGKVSVFATSGTEAEIAKSMMIDSITELEQGSIVDVKVVRIEKSIVELEFLNG
RKGKMHISEVANEHIDSIESVLKQDDTFKALVIDFEKGGCPKLSRRRVDQETGEFFEGELYNEERKDGPNDRDNYYNNSF
SRKPGGSHHKRPPRPRSGFSNRNRPKFGNNDSSSGFY
>Mature_757_residues
MFKIIKKSIEWGGRTLSLETGKIARQAHGSVVVNYGDTSVLVTVVSKKKEENVDFLPLNVQFIAKSYAMGKIPGGFFKRE
GKPSDRETLISRVIDRSIRPLFPEGFHDEISVVCNLLTYDTVNPPEVPALIGAVAALAISGVPFHFTIAGVMVGCDENNN
YILNPSVQEMKASSLDLFLSGDENSILMVESEVKELSEENVFNAIKFGHEHLKPVIKLIKEFADTIGNKPESFAPIDASD
ITQELEKYGKDFEKAYSQTVKQERVQALEAIRENILNTLKETGKDEKLITYAVKNFERSLVREIIRKKSVRIDGRKHDEI
RQIEVEVDILSKTHGSALFTRGNTQALVVTALGTTQDEQIVDDIEGDRREHFMLHYNFPSFAVGETSAARAPGRREIGHG
KLAWKAIHPVLPDKSEFPYTIRVVSEILESDGSSSMATVCGTSLALMDTGVPIKAPVAGIAMGLIKDKDEYVILSDILGD
EDYLGDMDFKVAGTSEGVTALQMDMKISGISFEIVEKSLEQAKAGRLHILEKMNAVISEHSDDVKDHAPRMLSFYIDKDK
ISAAIGSKGKNIRSVCERSNAKIEIGDDGKVSVFATSGTEAEIAKSMMIDSITELEQGSIVDVKVVRIEKSIVELEFLNG
RKGKMHISEVANEHIDSIESVLKQDDTFKALVIDFEKGGCPKLSRRRVDQETGEFFEGELYNEERKDGPNDRDNYYNNSF
SRKPGGSHHKRPPRPRSGFSNRNRPKFGNNDSSSGFY

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=660, Percent_Identity=37.1212121212121, Blast_Score=411, Evalue=1e-114,
Organism=Escherichia coli, GI145693187, Length=700, Percent_Identity=44.5714285714286, Blast_Score=590, Evalue=1e-169,
Organism=Caenorhabditis elegans, GI115534063, Length=644, Percent_Identity=34.1614906832298, Blast_Score=347, Evalue=2e-95,
Organism=Drosophila melanogaster, GI281362905, Length=651, Percent_Identity=35.7910906298003, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24651641, Length=651, Percent_Identity=35.7910906298003, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24651643, Length=651, Percent_Identity=35.7910906298003, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI161079377, Length=597, Percent_Identity=35.0083752093802, Blast_Score=361, Evalue=1e-99,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 84023; Mature: 84023

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIIKKSIEWGGRTLSLETGKIARQAHGSVVVNYGDTSVLVTVVSKKKEENVDFLPLNV
CHHHHHHHHHCCCEEEEEECCHHHHHCCCCEEEEECCCEEEEEEEHHHCCCCCCEEECHH
QFIAKSYAMGKIPGGFFKREGKPSDRETLISRVIDRSIRPLFPEGFHDEISVVCNLLTYD
HHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC
TVNPPEVPALIGAVAALAISGVPFHFTIAGVMVGCDENNNYILNPSVQEMKASSLDLFLS
CCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEECCCHHHHCCCCEEEEEE
GDENSILMVESEVKELSEENVFNAIKFGHEHLKPVIKLIKEFADTIGNKPESFAPIDASD
CCCCCEEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
ITQELEKYGKDFEKAYSQTVKQERVQALEAIRENILNTLKETGKDEKLITYAVKNFERSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VREIIRKKSVRIDGRKHDEIRQIEVEVDILSKTHGSALFTRGNTQALVVTALGTTQDEQI
HHHHHHHCCEECCCCCCCCEEEEEEEEEEECCCCCCEEEECCCCCEEEEEEECCCCHHHH
VDDIEGDRREHFMLHYNFPSFAVGETSAARAPGRREIGHGKLAWKAIHPVLPDKSEFPYT
HHHCCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCEEHHHHCCCCCCCCCCCCH
IRVVSEILESDGSSSMATVCGTSLALMDTGVPIKAPVAGIAMGLIKDKDEYVILSDILGD
HHHHHHHHHCCCCCCHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEHHHCCC
EDYLGDMDFKVAGTSEGVTALQMDMKISGISFEIVEKSLEQAKAGRLHILEKMNAVISEH
CCCCCCCCEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
SDDVKDHAPRMLSFYIDKDKISAAIGSKGKNIRSVCERSNAKIEIGDDGKVSVFATSGTE
CCCHHHHCCCEEEEEECHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCEEEEEEECCCH
AEIAKSMMIDSITELEQGSIVDVKVVRIEKSIVELEFLNGRKGKMHISEVANEHIDSIES
HHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHH
VLKQDDTFKALVIDFEKGGCPKLSRRRVDQETGEFFEGELYNEERKDGPNDRDNYYNNSF
HHCCCCCEEEEEEEECCCCCCCHHHHHCCHHHCCHHCCCCCCCHHCCCCCCCCCCCCCCC
SRKPGGSHHKRPPRPRSGFSNRNRPKFGNNDSSSGFY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFKIIKKSIEWGGRTLSLETGKIARQAHGSVVVNYGDTSVLVTVVSKKKEENVDFLPLNV
CHHHHHHHHHCCCEEEEEECCHHHHHCCCCEEEEECCCEEEEEEEHHHCCCCCCEEECHH
QFIAKSYAMGKIPGGFFKREGKPSDRETLISRVIDRSIRPLFPEGFHDEISVVCNLLTYD
HHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC
TVNPPEVPALIGAVAALAISGVPFHFTIAGVMVGCDENNNYILNPSVQEMKASSLDLFLS
CCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEECCCHHHHCCCCEEEEEE
GDENSILMVESEVKELSEENVFNAIKFGHEHLKPVIKLIKEFADTIGNKPESFAPIDASD
CCCCCEEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
ITQELEKYGKDFEKAYSQTVKQERVQALEAIRENILNTLKETGKDEKLITYAVKNFERSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VREIIRKKSVRIDGRKHDEIRQIEVEVDILSKTHGSALFTRGNTQALVVTALGTTQDEQI
HHHHHHHCCEECCCCCCCCEEEEEEEEEEECCCCCCEEEECCCCCEEEEEEECCCCHHHH
VDDIEGDRREHFMLHYNFPSFAVGETSAARAPGRREIGHGKLAWKAIHPVLPDKSEFPYT
HHHCCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCEEHHHHCCCCCCCCCCCCH
IRVVSEILESDGSSSMATVCGTSLALMDTGVPIKAPVAGIAMGLIKDKDEYVILSDILGD
HHHHHHHHHCCCCCCHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEHHHCCC
EDYLGDMDFKVAGTSEGVTALQMDMKISGISFEIVEKSLEQAKAGRLHILEKMNAVISEH
CCCCCCCCEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
SDDVKDHAPRMLSFYIDKDKISAAIGSKGKNIRSVCERSNAKIEIGDDGKVSVFATSGTE
CCCHHHHCCCEEEEEECHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCEEEEEEECCCH
AEIAKSMMIDSITELEQGSIVDVKVVRIEKSIVELEFLNGRKGKMHISEVANEHIDSIES
HHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHH
VLKQDDTFKALVIDFEKGGCPKLSRRRVDQETGEFFEGELYNEERKDGPNDRDNYYNNSF
HHCCCCCEEEEEEEECCCCCCCHHHHHCCHHHCCHHCCCCCCCHHCCCCCCCCCCCCCCC
SRKPGGSHHKRPPRPRSGFSNRNRPKFGNNDSSSGFY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA