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The map label for this gene is murD [H]

Identifier: 225630554

GI number: 225630554

Start: 874932

End: 876485

Strand: Direct

Name: murD [H]

Synonym: WRi_008130

Alternate gene names: 225630554

Gene position: 874932-876485 (Clockwise)

Preceding gene: 225630548

Following gene: 225630555

Centisome position: 60.51

GC content: 37.45

Gene sequence:

>1554_bases
ATGGTTCGCATAAGTTATCTAGCAGTGCAACTAAACAAATACAAAAATCAAAACGTCGCGGTTTTTGGTCTTGGTAAAAC
TGGTTTATCCGCCATTAACGCTCTAACAAAGAGTGGTGCAAGAATATATGCATGGGATGATCATGAAGAGCAAATAGCAA
ATGCAAAAATGATGTATAAAAAATGTAATTTTATTCATCCCAAGGAATACAATTGGCATGAGATCAGTGCATTGGTTTTA
AGCCCTGGAGTGCCAACAGAGCCACATTGGATAGTAAAACTTGCAAGGAGATTTGATTGCAAAATAAAATCAGACATTGA
GCTATTTCTTGAAGCTAAAACTACGAACCAGAAGGTAATAGGCGTCACAGGAACAAATGGTAAATCAACCACTACATCAC
TAATAGGGCACATATTAAAATCTGCAGGGAAAAAAGTAGCTATTGGTGGAAATTTAGGCGTTCCTGTTTTGGATCTAGAA
AGAGATGCAGAAATTTATGTAATCGAACTCTCCTCTTTTCAATTGGAGTTGATGAATGAAATTAACGTGGACATTTCAGC
ACTGCTCAATATTACACCAGATCACATAGATAGGCATGGAAGTATGGAGAACTACATAGCAACTAAATTAAAACTGATAA
ACGGTAGTGAGGTTGCCGTAATAGGATGTGACAATGAGATTACTGCTGATATATTCAACAAATTCACTGGAGACAAAATT
CCAATCTCAGTAACATATTCCCCGATGTCATTCCAGCGCGTGACGCTGGAATCCAGAAAAGAAAAACCATTGCCAACTAC
TCAGATGACAGAGGGTGGTGCAAGAGATCTAATATCATTGGCAGGTAACAATTTGCTTGATTATAGTGAACAGATTACTG
GTATAGATCGAAATTATCTGGATCCCAGTGTCAGCTACTTGGATGACAAAAGGGGCACTGGGATCCAGAAGATCTCGTTA
AAACTAGAGAATCATAGCTTATCAGTAAGCGACGTGAAAATAAACCTAATATCCAACGCAGAAAACATAGCAGCCACATA
TGCCGTATGTAAGGTACTTGGAGTAGATAGCAACACTATTATCAATGGAATCAAGTCCTTTTCAGGACTGAGGCATAGGA
ATGAACTGCTTGGCAAAATAAGAAATGTGTTTTTCGTGAACGATAGCAAAGCAACTAATGCAAAATCGAGCGAAAAGGCG
ATCTTATCTTATGAAAACATAAATTGGATCGCTGGTGGAAGAAGCAAAAAAGGTGGAATAGAATCATTAAGCAAGCATTT
TACAAGGGTTAGAAAAGCTTTTCTTATTGGAGAATCAACCGAAGCTTTTGCAAATGTTATGGAGAATAAAGTAGATTATG
TTAAATGTTGCAATCTAGAAGATGCATTCAGATTGGCTTTTGAAGAGGCCTTAAATAGCGCAGAAGAAGTAACAATATTA
CTTTCTCCCGCGTGTGCTTCTTTTGATCAATGGAAAAATTTCGAGGAACGTGGTGAAGCATTTTGTAGAATGTTTGAAAA
TCTCAGGTACAATCACACATGCTGTTTAGTATGA

Upstream 100 bases:

>100_bases
GAGGCTGGCGGAGTTTGTCAAGGAAGTTTTTTGTTTCTATTGAATGACAGTTGTAACCTACGAAAGAAGTCTGTTGTAAT
TTGTACGATATCTTTATAAA

Downstream 100 bases:

>100_bases
TGTTGCGTGGAACAAAAGTTAATAATAGATAAGCTAATTAGGGTTATTCCATTTGAAGGGATAAGCGATGAAACCCTATT
AAAGGTGTGCACTGACCTTA

Product: UDP-N-acetylmuramoylalanine-D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MVRISYLAVQLNKYKNQNVAVFGLGKTGLSAINALTKSGARIYAWDDHEEQIANAKMMYKKCNFIHPKEYNWHEISALVL
SPGVPTEPHWIVKLARRFDCKIKSDIELFLEAKTTNQKVIGVTGTNGKSTTTSLIGHILKSAGKKVAIGGNLGVPVLDLE
RDAEIYVIELSSFQLELMNEINVDISALLNITPDHIDRHGSMENYIATKLKLINGSEVAVIGCDNEITADIFNKFTGDKI
PISVTYSPMSFQRVTLESRKEKPLPTTQMTEGGARDLISLAGNNLLDYSEQITGIDRNYLDPSVSYLDDKRGTGIQKISL
KLENHSLSVSDVKINLISNAENIAATYAVCKVLGVDSNTIINGIKSFSGLRHRNELLGKIRNVFFVNDSKATNAKSSEKA
ILSYENINWIAGGRSKKGGIESLSKHFTRVRKAFLIGESTEAFANVMENKVDYVKCCNLEDAFRLAFEEALNSAEEVTIL
LSPACASFDQWKNFEERGEAFCRMFENLRYNHTCCLV

Sequences:

>Translated_517_residues
MVRISYLAVQLNKYKNQNVAVFGLGKTGLSAINALTKSGARIYAWDDHEEQIANAKMMYKKCNFIHPKEYNWHEISALVL
SPGVPTEPHWIVKLARRFDCKIKSDIELFLEAKTTNQKVIGVTGTNGKSTTTSLIGHILKSAGKKVAIGGNLGVPVLDLE
RDAEIYVIELSSFQLELMNEINVDISALLNITPDHIDRHGSMENYIATKLKLINGSEVAVIGCDNEITADIFNKFTGDKI
PISVTYSPMSFQRVTLESRKEKPLPTTQMTEGGARDLISLAGNNLLDYSEQITGIDRNYLDPSVSYLDDKRGTGIQKISL
KLENHSLSVSDVKINLISNAENIAATYAVCKVLGVDSNTIINGIKSFSGLRHRNELLGKIRNVFFVNDSKATNAKSSEKA
ILSYENINWIAGGRSKKGGIESLSKHFTRVRKAFLIGESTEAFANVMENKVDYVKCCNLEDAFRLAFEEALNSAEEVTIL
LSPACASFDQWKNFEERGEAFCRMFENLRYNHTCCLV
>Mature_517_residues
MVRISYLAVQLNKYKNQNVAVFGLGKTGLSAINALTKSGARIYAWDDHEEQIANAKMMYKKCNFIHPKEYNWHEISALVL
SPGVPTEPHWIVKLARRFDCKIKSDIELFLEAKTTNQKVIGVTGTNGKSTTTSLIGHILKSAGKKVAIGGNLGVPVLDLE
RDAEIYVIELSSFQLELMNEINVDISALLNITPDHIDRHGSMENYIATKLKLINGSEVAVIGCDNEITADIFNKFTGDKI
PISVTYSPMSFQRVTLESRKEKPLPTTQMTEGGARDLISLAGNNLLDYSEQITGIDRNYLDPSVSYLDDKRGTGIQKISL
KLENHSLSVSDVKINLISNAENIAATYAVCKVLGVDSNTIINGIKSFSGLRHRNELLGKIRNVFFVNDSKATNAKSSEKA
ILSYENINWIAGGRSKKGGIESLSKHFTRVRKAFLIGESTEAFANVMENKVDYVKCCNLEDAFRLAFEEALNSAEEVTIL
LSPACASFDQWKNFEERGEAFCRMFENLRYNHTCCLV

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=506, Percent_Identity=27.8656126482213, Blast_Score=163, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 57472; Mature: 57472

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRISYLAVQLNKYKNQNVAVFGLGKTGLSAINALTKSGARIYAWDDHEEQIANAKMMYK
CEEEEEEEEEEECCCCCCEEEEEECHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
KCNFIHPKEYNWHEISALVLSPGVPTEPHWIVKLARRFDCKIKSDIELFLEAKTTNQKVI
HCCCCCCCCCCHHEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEE
GVTGTNGKSTTTSLIGHILKSAGKKVAIGGNLGVPVLDLERDAEIYVIELSSFQLELMNE
EEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECCCCEEEEEEECCCHHHHHHH
INVDISALLNITPDHIDRHGSMENYIATKLKLINGSEVAVIGCDNEITADIFNKFTGDKI
CCCCEEHEEECCHHHHHCCCCHHHHHEEEEEEECCCEEEEEECCCCHHHHHHHHCCCCCE
PISVTYSPMSFQRVTLESRKEKPLPTTQMTEGGARDLISLAGNNLLDYSEQITGIDRNYL
EEEEEECCCCEEEEEHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCC
DPSVSYLDDKRGTGIQKISLKLENHSLSVSDVKINLISNAENIAATYAVCKVLGVDSNTI
CCCHHHHCCCCCCCEEEEEEEEECCEEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCHHH
INGIKSFSGLRHRNELLGKIRNVFFVNDSKATNAKSSEKAILSYENINWIAGGRSKKGGI
HHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEEECCEEEEECCCCCCCCH
ESLSKHFTRVRKAFLIGESTEAFANVMENKVDYVKCCNLEDAFRLAFEEALNSAEEVTIL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEE
LSPACASFDQWKNFEERGEAFCRMFENLRYNHTCCLV
ECCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MVRISYLAVQLNKYKNQNVAVFGLGKTGLSAINALTKSGARIYAWDDHEEQIANAKMMYK
CEEEEEEEEEEECCCCCCEEEEEECHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
KCNFIHPKEYNWHEISALVLSPGVPTEPHWIVKLARRFDCKIKSDIELFLEAKTTNQKVI
HCCCCCCCCCCHHEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEE
GVTGTNGKSTTTSLIGHILKSAGKKVAIGGNLGVPVLDLERDAEIYVIELSSFQLELMNE
EEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECCCCEEEEEEECCCHHHHHHH
INVDISALLNITPDHIDRHGSMENYIATKLKLINGSEVAVIGCDNEITADIFNKFTGDKI
CCCCEEHEEECCHHHHHCCCCHHHHHEEEEEEECCCEEEEEECCCCHHHHHHHHCCCCCE
PISVTYSPMSFQRVTLESRKEKPLPTTQMTEGGARDLISLAGNNLLDYSEQITGIDRNYL
EEEEEECCCCEEEEEHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCC
DPSVSYLDDKRGTGIQKISLKLENHSLSVSDVKINLISNAENIAATYAVCKVLGVDSNTI
CCCHHHHCCCCCCCEEEEEEEEECCEEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCHHH
INGIKSFSGLRHRNELLGKIRNVFFVNDSKATNAKSSEKAILSYENINWIAGGRSKKGGI
HHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEEECCEEEEECCCCCCCCH
ESLSKHFTRVRKAFLIGESTEAFANVMENKVDYVKCCNLEDAFRLAFEEALNSAEEVTIL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEE
LSPACASFDQWKNFEERGEAFCRMFENLRYNHTCCLV
ECCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA