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The map label for this gene is proS [H]

Identifier: 225630527

GI number: 225630527

Start: 840444

End: 841712

Strand: Direct

Name: proS [H]

Synonym: WRi_007800

Alternate gene names: 225630527

Gene position: 840444-841712 (Clockwise)

Preceding gene: 225630526

Following gene: 225630528

Centisome position: 58.13

GC content: 37.67

Gene sequence:

>1269_bases
ATGCGTTTATCCAAGTATTACTTACCAACTCTAAAGGAAAAGCCTGCCCATGCTAAAATTATCTCCCATCAATATTCTTT
ACGTGCAGGTTTAATAAAGCAGATAGCATCTGGCATTTATTCCTGGTTACCACTTGGTCTCTTAGTGCTTAAGAATATTG
AAGACATCATCAGAGATGAAATGGATAAATCTGGCGCTATTGAAGTGTTAATGCCTTGTGTCCAACCAGCAAATCTTTGG
CGAGAATCAGGGCGTTACGATGATTATGGTAAGGAAATGCTACGCATAAAGGATAGGCATGAGGAAGATATGCTTTTTGG
TCCAACGCATGAGGAGGTAGCAACTGATTTAATTAGAGATGTGGTAAAAAGTTACAAAGATTTGCCACTTTGTTTATACC
AAATTCAATGGAAGTTTCGTGATGAGGTAAGACCACGTTATGGTGTTATGAGGGGCAGGGAATTTTTGATGAAGGATGCA
TACAGTTTTGATGTAGATTACGAAGGTGCACTGAATTCATATAATTTGATGTACAAAACTTACATAAAAATCTTCAAACG
CATGGGACTTACCCCAATTGGAGTGAGAGCTGATACGGGGCCAATTGGAGGAAATTTGAGCCACGAGTTTCATATATTGG
CAAACACTGGTGAAAGCACTTTATATTACGACAACAAATTTTCTGAGTTGCTAGAAAGCGAAGATGTTGAAAGTTTGAAG
AGTATATACGCAGTTGCAGATGATATGCATGATCCTGAGACTTGCCCTATATCACAAGAGCAGTTAAATGTTAGTAAAGG
TATTGAAATAGGGCATATTTTTTATTTTGGCGACAAATACTCAAAGCCTATGAAGGCAAGTGTTACTTCTCAAGATGGAA
AAAATGTCAATATACATATGGGTTCGTATGGCATTGGAGTTTCAAGGCTTGTCGGTGCAATAATAGAAGCTTTTCATGAT
GATAAAGGAATTATCTGGCCAGAATCGGTGGCTCCTTTCAGAATTGGTTTAATCAATTTACAAACGAAAGTAACGGAGGC
TGCAGATAAAATATACAAAGCTTTGAAGAGCGATGAAGTGCTTTACGATGACACGGAAGGAAGTGTAGGAGTAAAATTTT
CTAGAATGGATCTGATAGGCTTGCCGTGGCAAATAATTGTCGGAAAAAAGGCAATAGATGAAAAGATAGTTGAAGTAAAA
AATAGAGCAACTGGAGAGGTAAAGGAAATGCAGATTGAGGAAGCAATAAATCACTTTAGCACAAAATGA

Upstream 100 bases:

>100_bases
CTATATGAAATGGTCAAGACGCACTTCCCTTCTTTTTCAAAATAAGTTTACCTACAGGTCTGATTGTATTAAGATTACTT
AATACAATCAGATCTATTAT

Downstream 100 bases:

>100_bases
TACACGGTATCGGCACTGACATAGTATATATACCAAGAGTATCGAGGATATTACAAAAATACGGGGAAAAATTTCTCAAT
AGAATCTACACTGAGAAAGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MRLSKYYLPTLKEKPAHAKIISHQYSLRAGLIKQIASGIYSWLPLGLLVLKNIEDIIRDEMDKSGAIEVLMPCVQPANLW
RESGRYDDYGKEMLRIKDRHEEDMLFGPTHEEVATDLIRDVVKSYKDLPLCLYQIQWKFRDEVRPRYGVMRGREFLMKDA
YSFDVDYEGALNSYNLMYKTYIKIFKRMGLTPIGVRADTGPIGGNLSHEFHILANTGESTLYYDNKFSELLESEDVESLK
SIYAVADDMHDPETCPISQEQLNVSKGIEIGHIFYFGDKYSKPMKASVTSQDGKNVNIHMGSYGIGVSRLVGAIIEAFHD
DKGIIWPESVAPFRIGLINLQTKVTEAADKIYKALKSDEVLYDDTEGSVGVKFSRMDLIGLPWQIIVGKKAIDEKIVEVK
NRATGEVKEMQIEEAINHFSTK

Sequences:

>Translated_422_residues
MRLSKYYLPTLKEKPAHAKIISHQYSLRAGLIKQIASGIYSWLPLGLLVLKNIEDIIRDEMDKSGAIEVLMPCVQPANLW
RESGRYDDYGKEMLRIKDRHEEDMLFGPTHEEVATDLIRDVVKSYKDLPLCLYQIQWKFRDEVRPRYGVMRGREFLMKDA
YSFDVDYEGALNSYNLMYKTYIKIFKRMGLTPIGVRADTGPIGGNLSHEFHILANTGESTLYYDNKFSELLESEDVESLK
SIYAVADDMHDPETCPISQEQLNVSKGIEIGHIFYFGDKYSKPMKASVTSQDGKNVNIHMGSYGIGVSRLVGAIIEAFHD
DKGIIWPESVAPFRIGLINLQTKVTEAADKIYKALKSDEVLYDDTEGSVGVKFSRMDLIGLPWQIIVGKKAIDEKIVEVK
NRATGEVKEMQIEEAINHFSTK
>Mature_422_residues
MRLSKYYLPTLKEKPAHAKIISHQYSLRAGLIKQIASGIYSWLPLGLLVLKNIEDIIRDEMDKSGAIEVLMPCVQPANLW
RESGRYDDYGKEMLRIKDRHEEDMLFGPTHEEVATDLIRDVVKSYKDLPLCLYQIQWKFRDEVRPRYGVMRGREFLMKDA
YSFDVDYEGALNSYNLMYKTYIKIFKRMGLTPIGVRADTGPIGGNLSHEFHILANTGESTLYYDNKFSELLESEDVESLK
SIYAVADDMHDPETCPISQEQLNVSKGIEIGHIFYFGDKYSKPMKASVTSQDGKNVNIHMGSYGIGVSRLVGAIIEAFHD
DKGIIWPESVAPFRIGLINLQTKVTEAADKIYKALKSDEVLYDDTEGSVGVKFSRMDLIGLPWQIIVGKKAIDEKIVEVK
NRATGEVKEMQIEEAINHFSTK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=414, Percent_Identity=38.1642512077295, Blast_Score=273, Evalue=3e-73,
Organism=Escherichia coli, GI1786392, Length=225, Percent_Identity=60.4444444444444, Blast_Score=294, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI115532348, Length=403, Percent_Identity=32.258064516129, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6320931, Length=193, Percent_Identity=41.9689119170984, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24656200, Length=402, Percent_Identity=32.5870646766169, Blast_Score=238, Evalue=6e-63,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 48031; Mature: 48031

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSKYYLPTLKEKPAHAKIISHQYSLRAGLIKQIASGIYSWLPLGLLVLKNIEDIIRDE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDKSGAIEVLMPCVQPANLWRESGRYDDYGKEMLRIKDRHEEDMLFGPTHEEVATDLIRD
CCCCCCHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHH
VVKSYKDLPLCLYQIQWKFRDEVRPRYGVMRGREFLMKDAYSFDVDYEGALNSYNLMYKT
HHHHHCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
YIKIFKRMGLTPIGVRADTGPIGGNLSHEFHILANTGESTLYYDNKFSELLESEDVESLK
HHHHHHHCCCCEECEECCCCCCCCCCCCEEEEEEECCCCEEEECCHHHHHHHHCCHHHHH
SIYAVADDMHDPETCPISQEQLNVSKGIEIGHIFYFGDKYSKPMKASVTSQDGKNVNIHM
HHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEE
GSYGIGVSRLVGAIIEAFHDDKGIIWPESVAPFRIGLINLQTKVTEAADKIYKALKSDEV
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCE
LYDDTEGSVGVKFSRMDLIGLPWQIIVGKKAIDEKIVEVKNRATGEVKEMQIEEAINHFS
EEECCCCCCEEEEECEEEECCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
TK
CC
>Mature Secondary Structure
MRLSKYYLPTLKEKPAHAKIISHQYSLRAGLIKQIASGIYSWLPLGLLVLKNIEDIIRDE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDKSGAIEVLMPCVQPANLWRESGRYDDYGKEMLRIKDRHEEDMLFGPTHEEVATDLIRD
CCCCCCHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHH
VVKSYKDLPLCLYQIQWKFRDEVRPRYGVMRGREFLMKDAYSFDVDYEGALNSYNLMYKT
HHHHHCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
YIKIFKRMGLTPIGVRADTGPIGGNLSHEFHILANTGESTLYYDNKFSELLESEDVESLK
HHHHHHHCCCCEECEECCCCCCCCCCCCEEEEEEECCCCEEEECCHHHHHHHHCCHHHHH
SIYAVADDMHDPETCPISQEQLNVSKGIEIGHIFYFGDKYSKPMKASVTSQDGKNVNIHM
HHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEE
GSYGIGVSRLVGAIIEAFHDDKGIIWPESVAPFRIGLINLQTKVTEAADKIYKALKSDEV
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCE
LYDDTEGSVGVKFSRMDLIGLPWQIIVGKKAIDEKIVEVKNRATGEVKEMQIEEAINHFS
EEECCCCCCEEEEECEEEECCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
TK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA