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The map label for this gene is iscS [H]

Identifier: 225630412

GI number: 225630412

Start: 698327

End: 699463

Strand: Reverse

Name: iscS [H]

Synonym: WRi_006450

Alternate gene names: 225630412

Gene position: 699463-698327 (Counterclockwise)

Preceding gene: 225630414

Following gene: 225630411

Centisome position: 48.38

GC content: 37.64

Gene sequence:

>1137_bases
ATGAGTCCATTCTCTTTAGAAAATAGTGGTTGCGTATATGCTGATTACAATGCAACTGCTCCAATTAGTGAAAATGTAAA
AAAAAGTATATTTGAGGTCTTGTTAAAACAAACGCTCAATCCATCATCACTACATAAAAGAGGACAAGAAGCGAGGAAGA
TTCTTCAGGATGCAAGAGATAACGTACGTGGTGCTATTGGCGTCTCAAGTGATAAAGAAATAGTTTTCACGTCTGGTGCA
ACCGAAGCGAATAACCTTGTTATGAGAGGAATAGCAGGCTACCTGCATGTAATTTCAGCTATAGAGCATCCTTCAATTCT
TAATTCTGCATGTAATCCATACATAATACCCGTTAATCAGGAGGGCATTGTTGACTTTTTAGAGCTAGAAAAAATTCTAA
GCGAACTTAAAGGAAACAAAGCAATAGTTTCAGTTATGATGGCAAATAACGAAACCGGAGTTATTCAACCTATTGAGGAA
ATAGCTGAAATAGCCCACAAATTTGGAGCAATTTGCCACACTGACACTGCTCAAAGTGTTGGGAAAATTAAAGTTAATAT
GGAAGATTTAGGAGTGGATTTACTCACTTTATCCGCCCATAAATTTGGCGGTGTAGCAGGAAGTGGAGTTTTAATATTCA
ATAAAGAACTTGCGATAGAACCGATTATAATAGGTGGTGGACAAGAGAAGGGATTTCGTGGTGGTACGGAAAATATTGTT
GCGATTGCAGGCCTTTCTGCTGCACTGCAAAATATTCCAGACCTTCTATCAAAAATGGATGAAGTAAAGGAGCTACGTGA
TCAATTAGAGTGTGAATTATTAAATTTTGCCAGTGACATAAGAATCTTCGGTAAAAACTCTAAGAGGCTGCCAAACACAA
GTTTTATTTATATGCCAGGAGTAAAGAGTGATGTGCAGCTCATGCATTTTGACTTGAATTATATTGCAGTTAGTAATGGC
TCCGCATGTTCTTCTGGAAAAGTTGAGCCTTCCCATGTTTTGCTTGCAATGGGGGCAACAAAAGAGCAAGCAGAGTGTTC
AATTAGAATCAGTATAGGTCCAGAAACTAAACCACAAGACATAAAAAAAATAGTGGATTGTTGGTATAATATCTACAAGC
AGAACACCTTGGTATAG

Upstream 100 bases:

>100_bases
ATGTATTAATTTCGCTGTTTTTGCAATAAATTTTTTATCGTGTTAAAGCTAACGTACATAGTTATGGCTATAGTTGATGG
TAAACTTATTTAAGTGACAG

Downstream 100 bases:

>100_bases
ATAATACGTGATATCATGCAGGGAGCTATAAATTAGATGTAAAAAATTCCTTTACAAGTTTCGCCAGCCCCCTTATCATA
ACAGTGAAGCTATTTATTTA

Product: aminotransferase, class V

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSPFSLENSGCVYADYNATAPISENVKKSIFEVLLKQTLNPSSLHKRGQEARKILQDARDNVRGAIGVSSDKEIVFTSGA
TEANNLVMRGIAGYLHVISAIEHPSILNSACNPYIIPVNQEGIVDFLELEKILSELKGNKAIVSVMMANNETGVIQPIEE
IAEIAHKFGAICHTDTAQSVGKIKVNMEDLGVDLLTLSAHKFGGVAGSGVLIFNKELAIEPIIIGGGQEKGFRGGTENIV
AIAGLSAALQNIPDLLSKMDEVKELRDQLECELLNFASDIRIFGKNSKRLPNTSFIYMPGVKSDVQLMHFDLNYIAVSNG
SACSSGKVEPSHVLLAMGATKEQAECSIRISIGPETKPQDIKKIVDCWYNIYKQNTLV

Sequences:

>Translated_378_residues
MSPFSLENSGCVYADYNATAPISENVKKSIFEVLLKQTLNPSSLHKRGQEARKILQDARDNVRGAIGVSSDKEIVFTSGA
TEANNLVMRGIAGYLHVISAIEHPSILNSACNPYIIPVNQEGIVDFLELEKILSELKGNKAIVSVMMANNETGVIQPIEE
IAEIAHKFGAICHTDTAQSVGKIKVNMEDLGVDLLTLSAHKFGGVAGSGVLIFNKELAIEPIIIGGGQEKGFRGGTENIV
AIAGLSAALQNIPDLLSKMDEVKELRDQLECELLNFASDIRIFGKNSKRLPNTSFIYMPGVKSDVQLMHFDLNYIAVSNG
SACSSGKVEPSHVLLAMGATKEQAECSIRISIGPETKPQDIKKIVDCWYNIYKQNTLV
>Mature_377_residues
SPFSLENSGCVYADYNATAPISENVKKSIFEVLLKQTLNPSSLHKRGQEARKILQDARDNVRGAIGVSSDKEIVFTSGAT
EANNLVMRGIAGYLHVISAIEHPSILNSACNPYIIPVNQEGIVDFLELEKILSELKGNKAIVSVMMANNETGVIQPIEEI
AEIAHKFGAICHTDTAQSVGKIKVNMEDLGVDLLTLSAHKFGGVAGSGVLIFNKELAIEPIIIGGGQEKGFRGGTENIVA
IAGLSAALQNIPDLLSKMDEVKELRDQLECELLNFASDIRIFGKNSKRLPNTSFIYMPGVKSDVQLMHFDLNYIAVSNGS
ACSSGKVEPSHVLLAMGATKEQAECSIRISIGPETKPQDIKKIVDCWYNIYKQNTLV

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=376, Percent_Identity=33.5106382978723, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI156713448, Length=422, Percent_Identity=30.3317535545024, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI48994898, Length=381, Percent_Identity=33.5958005249344, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1787970, Length=314, Percent_Identity=26.4331210191083, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789175, Length=398, Percent_Identity=24.3718592964824, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25143064, Length=381, Percent_Identity=34.6456692913386, Blast_Score=187, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17533177, Length=317, Percent_Identity=35.6466876971609, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319831, Length=389, Percent_Identity=34.1902313624679, Blast_Score=189, Evalue=8e-49,
Organism=Drosophila melanogaster, GI20129463, Length=379, Percent_Identity=34.3007915567282, Blast_Score=182, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40960; Mature: 40828

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPFSLENSGCVYADYNATAPISENVKKSIFEVLLKQTLNPSSLHKRGQEARKILQDARD
CCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
NVRGAIGVSSDKEIVFTSGATEANNLVMRGIAGYLHVISAIEHPSILNSACNPYIIPVNQ
CCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCC
EGIVDFLELEKILSELKGNKAIVSVMMANNETGVIQPIEEIAEIAHKFGAICHTDTAQSV
CCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCEEECCCHHCC
GKIKVNMEDLGVDLLTLSAHKFGGVAGSGVLIFNKELAIEPIIIGGGQEKGFRGGTENIV
CEEEEEHHHHCEEEEEECHHHCCCCCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCEE
AIAGLSAALQNIPDLLSKMDEVKELRDQLECELLNFASDIRIFGKNSKRLPNTSFIYMPG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEECC
VKSDVQLMHFDLNYIAVSNGSACSSGKVEPSHVLLAMGATKEQAECSIRISIGPETKPQD
CCCCCEEEEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCHHH
IKKIVDCWYNIYKQNTLV
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SPFSLENSGCVYADYNATAPISENVKKSIFEVLLKQTLNPSSLHKRGQEARKILQDARD
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
NVRGAIGVSSDKEIVFTSGATEANNLVMRGIAGYLHVISAIEHPSILNSACNPYIIPVNQ
CCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCC
EGIVDFLELEKILSELKGNKAIVSVMMANNETGVIQPIEEIAEIAHKFGAICHTDTAQSV
CCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCEEECCCHHCC
GKIKVNMEDLGVDLLTLSAHKFGGVAGSGVLIFNKELAIEPIIIGGGQEKGFRGGTENIV
CEEEEEHHHHCEEEEEECHHHCCCCCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCEE
AIAGLSAALQNIPDLLSKMDEVKELRDQLECELLNFASDIRIFGKNSKRLPNTSFIYMPG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEECC
VKSDVQLMHFDLNYIAVSNGSACSSGKVEPSHVLLAMGATKEQAECSIRISIGPETKPQD
CCCCCEEEEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCHHH
IKKIVDCWYNIYKQNTLV
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA