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The map label for this gene is purB [H]

Identifier: 225630397

GI number: 225630397

Start: 675486

End: 676778

Strand: Direct

Name: purB [H]

Synonym: WRi_006260

Alternate gene names: 225630397

Gene position: 675486-676778 (Clockwise)

Preceding gene: 225630396

Following gene: 225630403

Centisome position: 46.72

GC content: 35.19

Gene sequence:

>1293_bases
ATGATTCCCCGCTATAGCAGAAAAGAAATGTCTTCCATTTGGGAGGAAAATAATAAATTTAATATATGGCTTAAAATAGA
AAAATTAGCGTGTGAAGCTCAAGCAAAGTTAGGAGTGATTCCGAATAATATTGCTGAGAACCTCTCTGGTGCTATTGAAT
TTGATGTTGAATGTATCAACGAAATTGAATCCATTGTAAAACATGATGTTATAGCTTTTCTGACATATATTGCTAAAAAA
GCGGGAGTTGATGTTCGTTATCTTCATTATGGAATGACAAGTTCTGATGTTTTGGATACATGCCTTGCGGTGCAACTGAA
AGAGTCCTGTGATATTTTGCTCGAGAATCTAAAAAATTTACTTGCAGCATTGAAAAAAAAAGCTGAGGACTATAAAGATA
TTGTTTGTGTTGGACGCAGTCACGGAATGCATGCAGAACCAACAACTCTTGGAATAAAATTTGCCAGATTTTATGCTGAA
TTTAAACGCAATTATCAGAGATTAATTAGCGCGCAAAAAGAGATCTCAATTTGTAAAATATCAGGTGCAGTAGGCAATTT
TGCAAATGTTGATCCGTTTGTTGAAGAATATGTAGCGAAAGAAATAGGACTCATACCTGAAATCATATCGTCTCAAGTCA
TTCCTCGTGATAGACATGCTATGTTCTTTTCAGTTTTGGGAGTGATTGCAAGCTCAATAGAAAATGTTGCTGTTGAAATT
CGTCATTTGCAAAGAACTGAAATCGGCGAAATTTCTGAGCATTTTTCCACGGGGCAGAAGGGAAGCTCTGCTATGCCACA
CAAATGTAATCCTATTTTAAGTGAAAATCTGACTGGACTCTCACGTTTAATACGCAGTTATGTTTTTCCTGCGTTAGAAA
ATGTTGCATTGTGGCACGAACGTGATATATCACACTCGTCTGTGGAAAGATGCATTGCTCCTGATGCTTGTATAACAATG
GATTTTGCTTTAGTGCGATTAACAGATTTGATAGATAAATTGGTGATCAATAAGGAAAACATTGAAAAGAACTTAAATTC
TTCAAAAGGTTTAGTTTTCTCACAGCGTGTATTGCTTGAGTTAGTAAATAGTGGTTTGGCGAGGGAAGAGGCGTATAAAA
TTGTTCAGAGCAATGCAATGAAAGTGAAACAAAACAACAGTGATTTTCTGGCCGAATTGAAACAAGATAAACCCTTACTT
AAGGTTATTAACTCGGAAAAACTTGAATCTTTGTTTGATTTGAAGTATTATACGAAACATATAGATCGTGTGTATAATAA
GGTTTTTAATTAG

Upstream 100 bases:

>100_bases
CTTCTAGAATCCGTTGTCCATTGAATAATTTGCAAATACTTTTATACCTGGATTCCAGTGTCACGCACTGGAATGACACC
ATTATTGGAATAAATCAAAA

Downstream 100 bases:

>100_bases
GCTGAGTAGCTCAGTTGGTAGAGCAGGAGGATCATAATCTCTTGGTCGGGGGTTCAAGTCCCTCCTCAGCCACTCGAACT
AAACTATGTTTAGCAAAGCA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MIPRYSRKEMSSIWEENNKFNIWLKIEKLACEAQAKLGVIPNNIAENLSGAIEFDVECINEIESIVKHDVIAFLTYIAKK
AGVDVRYLHYGMTSSDVLDTCLAVQLKESCDILLENLKNLLAALKKKAEDYKDIVCVGRSHGMHAEPTTLGIKFARFYAE
FKRNYQRLISAQKEISICKISGAVGNFANVDPFVEEYVAKEIGLIPEIISSQVIPRDRHAMFFSVLGVIASSIENVAVEI
RHLQRTEIGEISEHFSTGQKGSSAMPHKCNPILSENLTGLSRLIRSYVFPALENVALWHERDISHSSVERCIAPDACITM
DFALVRLTDLIDKLVINKENIEKNLNSSKGLVFSQRVLLELVNSGLAREEAYKIVQSNAMKVKQNNSDFLAELKQDKPLL
KVINSEKLESLFDLKYYTKHIDRVYNKVFN

Sequences:

>Translated_430_residues
MIPRYSRKEMSSIWEENNKFNIWLKIEKLACEAQAKLGVIPNNIAENLSGAIEFDVECINEIESIVKHDVIAFLTYIAKK
AGVDVRYLHYGMTSSDVLDTCLAVQLKESCDILLENLKNLLAALKKKAEDYKDIVCVGRSHGMHAEPTTLGIKFARFYAE
FKRNYQRLISAQKEISICKISGAVGNFANVDPFVEEYVAKEIGLIPEIISSQVIPRDRHAMFFSVLGVIASSIENVAVEI
RHLQRTEIGEISEHFSTGQKGSSAMPHKCNPILSENLTGLSRLIRSYVFPALENVALWHERDISHSSVERCIAPDACITM
DFALVRLTDLIDKLVINKENIEKNLNSSKGLVFSQRVLLELVNSGLAREEAYKIVQSNAMKVKQNNSDFLAELKQDKPLL
KVINSEKLESLFDLKYYTKHIDRVYNKVFN
>Mature_430_residues
MIPRYSRKEMSSIWEENNKFNIWLKIEKLACEAQAKLGVIPNNIAENLSGAIEFDVECINEIESIVKHDVIAFLTYIAKK
AGVDVRYLHYGMTSSDVLDTCLAVQLKESCDILLENLKNLLAALKKKAEDYKDIVCVGRSHGMHAEPTTLGIKFARFYAE
FKRNYQRLISAQKEISICKISGAVGNFANVDPFVEEYVAKEIGLIPEIISSQVIPRDRHAMFFSVLGVIASSIENVAVEI
RHLQRTEIGEISEHFSTGQKGSSAMPHKCNPILSENLTGLSRLIRSYVFPALENVALWHERDISHSSVERCIAPDACITM
DFALVRLTDLIDKLVINKENIEKNLNSSKGLVFSQRVLLELVNSGLAREEAYKIVQSNAMKVKQNNSDFLAELKQDKPLL
KVINSEKLESLFDLKYYTKHIDRVYNKVFN

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=438, Percent_Identity=26.4840182648402, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI183227688, Length=407, Percent_Identity=26.5356265356265, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1787376, Length=321, Percent_Identity=28.9719626168224, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787896, Length=200, Percent_Identity=30.5, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508577, Length=397, Percent_Identity=27.455919395466, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32564234, Length=357, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17553882, Length=304, Percent_Identity=25.3289473684211, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI32565146, Length=298, Percent_Identity=25.8389261744966, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323391, Length=433, Percent_Identity=25.8660508083141, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324993, Length=175, Percent_Identity=30.2857142857143, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24647570, Length=442, Percent_Identity=25.1131221719457, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24640179, Length=311, Percent_Identity=27.0096463022508, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24640177, Length=311, Percent_Identity=27.0096463022508, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78710009, Length=279, Percent_Identity=26.5232974910394, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48632; Mature: 48632

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSRKEMSSIWEENNKFNIWLKIEKLACEAQAKLGVIPNNIAENLSGAIEFDVECIN
CCCCCCHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHH
EIESIVKHDVIAFLTYIAKKAGVDVRYLHYGMTSSDVLDTCLAVQLKESCDILLENLKNL
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAALKKKAEDYKDIVCVGRSHGMHAEPTTLGIKFARFYAEFKRNYQRLISAQKEISICKI
HHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
SGAVGNFANVDPFVEEYVAKEIGLIPEIISSQVIPRDRHAMFFSVLGVIASSIENVAVEI
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEIGEISEHFSTGQKGSSAMPHKCNPILSENLTGLSRLIRSYVFPALENVALWHE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERCIAPDACITMDFALVRLTDLIDKLVINKENIEKNLNSSKGLVFSQRVLLE
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHH
LVNSGLAREEAYKIVQSNAMKVKQNNSDFLAELKQDKPLLKVINSEKLESLFDLKYYTKH
HHHCCCHHHHHHHHHHHCCHHEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IDRVYNKVFN
HHHHHHHHCC
>Mature Secondary Structure
MIPRYSRKEMSSIWEENNKFNIWLKIEKLACEAQAKLGVIPNNIAENLSGAIEFDVECIN
CCCCCCHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHH
EIESIVKHDVIAFLTYIAKKAGVDVRYLHYGMTSSDVLDTCLAVQLKESCDILLENLKNL
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAALKKKAEDYKDIVCVGRSHGMHAEPTTLGIKFARFYAEFKRNYQRLISAQKEISICKI
HHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
SGAVGNFANVDPFVEEYVAKEIGLIPEIISSQVIPRDRHAMFFSVLGVIASSIENVAVEI
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEIGEISEHFSTGQKGSSAMPHKCNPILSENLTGLSRLIRSYVFPALENVALWHE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERCIAPDACITMDFALVRLTDLIDKLVINKENIEKNLNSSKGLVFSQRVLLE
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHH
LVNSGLAREEAYKIVQSNAMKVKQNNSDFLAELKQDKPLLKVINSEKLESLFDLKYYTKH
HHHCCCHHHHHHHHHHHCCHHEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IDRVYNKVFN
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571; 10673438 [H]