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The map label for this gene is ligA [H]

Identifier: 225630391

GI number: 225630391

Start: 666044

End: 668032

Strand: Reverse

Name: ligA [H]

Synonym: WRi_006200

Alternate gene names: 225630391

Gene position: 668032-666044 (Counterclockwise)

Preceding gene: 225630398

Following gene: 225630382

Centisome position: 46.2

GC content: 35.85

Gene sequence:

>1989_bases
ATGACTAACTTAGAAAAGATGAGAGAAAAACTGCAAGATCAAATAAATTATCATAATGTTTTGTATCATCAAAAAAATAA
GCCAGAAATAAGTGATGCTGAATATGATGAACTGAAGAAAAAGTTAGCTGAAATAGAGCCAGAAATTTATGCAACACAAG
AAAGTGTTGGTGCTCCACCTGATGAGAGGTTTTCTAAGGTGGAACATCAAGAACCTATGCTTTCCCTTGAGAATGCTTAT
GATGAACAAGGTGTAGAGAAATTTTTGTCTAAAATAAAGAGGTTTTTAATTGAGGACAAAATAGAGATATTATGTGAACC
AAAAATTGATGGGTTGTCGTTTTCTGCAATTTATGAGGATGGAAGATTTGTTAAAGCTGCAACTCGAGGTGATGGTTTTG
TAGGGGAAGATGTCACTCACAATGTTGCAACAATAAAAGGCTTTCCTAAGTTTTTGCAAGGTGTGCAAGGAAGACTAGAA
GTAAGAGGTGAAATATACATCAGTAACAGCGACTTTTTAAAGTTAAATGAAAACGATGAATTTGCCAATCCTCGAAATGC
AGCCGCTGGTTCTCTAAAGCAATTGGATGCAAACATTACAGCAAGCAGACCTCTTAGATATTTTGCTTATTCTTTGATTG
GTGGAGCAGAGAAAAGTCAAAGTGAGGTACTAAATAAGCTTGAGGCACTTGGTTTTTGCGTAAATGAGCATCAGTCCTTA
ACGAGTAGTTTGGATGGAATGCTGAAGTTCTATAACGAAGTCTATAACTGTCGCTATAACTTGGATTATGATATTGATGG
AATAGTCTATAAAGTAAATGATTTGGTACTACAAAATCGTCTAGGGAATACTCACAAAGCACCGCGCTCAGCACTTGCAT
ACAAGTTTTCTGCGGTTTATGCAAAAACAAAGTTAAATAAAATATTTATACAGGTGGGCAGAACAGGAGTTTTAACTCCA
GTTGCAGATTTAGTGCCAGTTAATATTGGTGGGGTGCTAGTTAGTAGAGCAAGTCTGCATAACCAAGATGAGATAAAACG
TAAAGACATAAGAGAGGGAGATATTGTAACAATCAAAAGGGCAGGGGATGTAATTCCCCAGATCGTTGAAGTAAGCAGAG
ATTCTCGTCTTCCAAATACACCTGAATTTGTGTTTCCTGAAGTATGCCCTGAATGTGGTAGTAAAGTACGTCAGGTTGAG
GGAGAGGCTGCAGTAAGATGTCCTGAGGAATTTGCCTGTAAAGCTCAAATAATAGAAAAACTAAAGCATTTTGTATCGAA
AGATGCATTTGACATTGTTGGCCTTGGTGATAAGCAGATAGAGTTTTTTTACGACCTTGGTCTGATAAAACAAATTCCCG
ATATTTTTACTTTAGAAGAAAGATTGGATGAATTTAATCTGGAAGAACAGTCTGGTTGGGGCAAGAAATCAATAGCTCAT
TTATTAAATGCTATACAAAGCAGAAGGGTAATAACTCTAGATAGGTTTATATTTTCTTTAGGTATTAGATTTATTGGCCA
AGTTGCAGCAGAATTGCTTGCAGATTATTATGTTTCTTATGATAATTGGTATAATTCGATGATTGAACTGTCATCAGATA
ATACTGAGCTAGTAGGTATAGACGGCATAGGAAAAAAAGTAGCTGAATCTTTGGAATCATTTTTTTCTAAGGAACATAAC
ATCAAAATGTTAAATGATCTTACTGCTTGTCTTCAAATTCTTCCTGTGAGCTCCAACTCTAGTAACTCTGTTTTAAATAA
TAAAATTATAGTGTTTACTGGTAAGCTACTTGCTATGAGTAGAGGAGAGGCAAAAGTAAGAGCCAAAACTCTAGGAGCAA
AGGTCAGCTCACATCTTTCTGCTAAAACTGACTACCTAATTGCAGGAGAAAAACCAGGATCCAAATATAAAAAGGCAGTG
GAATTAGGTGTAGAAATTTTAGATGAAGACCAGTGGCATAAAGTGATAAGTTTGGGAGTGTTCAAATGA

Upstream 100 bases:

>100_bases
ATTGTGGCACCTTTGATATTTGATGGATTAAGACCATATGATAAATTCAAGAAAATTCACAGCTTGACGACAAAGGCTAA
TGTAGCATTATATTGGTATC

Downstream 100 bases:

>100_bases
AACTTGGATTGTATATTAAATCAAAGTTGCAAGACTATTTAGAGAGTAGCCAATTCTTTCCATCCAAGAATCATTTTATT
GACTTGCCTCAATTTTTCAA

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MTNLEKMREKLQDQINYHNVLYHQKNKPEISDAEYDELKKKLAEIEPEIYATQESVGAPPDERFSKVEHQEPMLSLENAY
DEQGVEKFLSKIKRFLIEDKIEILCEPKIDGLSFSAIYEDGRFVKAATRGDGFVGEDVTHNVATIKGFPKFLQGVQGRLE
VRGEIYISNSDFLKLNENDEFANPRNAAAGSLKQLDANITASRPLRYFAYSLIGGAEKSQSEVLNKLEALGFCVNEHQSL
TSSLDGMLKFYNEVYNCRYNLDYDIDGIVYKVNDLVLQNRLGNTHKAPRSALAYKFSAVYAKTKLNKIFIQVGRTGVLTP
VADLVPVNIGGVLVSRASLHNQDEIKRKDIREGDIVTIKRAGDVIPQIVEVSRDSRLPNTPEFVFPEVCPECGSKVRQVE
GEAAVRCPEEFACKAQIIEKLKHFVSKDAFDIVGLGDKQIEFFYDLGLIKQIPDIFTLEERLDEFNLEEQSGWGKKSIAH
LLNAIQSRRVITLDRFIFSLGIRFIGQVAAELLADYYVSYDNWYNSMIELSSDNTELVGIDGIGKKVAESLESFFSKEHN
IKMLNDLTACLQILPVSSNSSNSVLNNKIIVFTGKLLAMSRGEAKVRAKTLGAKVSSHLSAKTDYLIAGEKPGSKYKKAV
ELGVEILDEDQWHKVISLGVFK

Sequences:

>Translated_662_residues
MTNLEKMREKLQDQINYHNVLYHQKNKPEISDAEYDELKKKLAEIEPEIYATQESVGAPPDERFSKVEHQEPMLSLENAY
DEQGVEKFLSKIKRFLIEDKIEILCEPKIDGLSFSAIYEDGRFVKAATRGDGFVGEDVTHNVATIKGFPKFLQGVQGRLE
VRGEIYISNSDFLKLNENDEFANPRNAAAGSLKQLDANITASRPLRYFAYSLIGGAEKSQSEVLNKLEALGFCVNEHQSL
TSSLDGMLKFYNEVYNCRYNLDYDIDGIVYKVNDLVLQNRLGNTHKAPRSALAYKFSAVYAKTKLNKIFIQVGRTGVLTP
VADLVPVNIGGVLVSRASLHNQDEIKRKDIREGDIVTIKRAGDVIPQIVEVSRDSRLPNTPEFVFPEVCPECGSKVRQVE
GEAAVRCPEEFACKAQIIEKLKHFVSKDAFDIVGLGDKQIEFFYDLGLIKQIPDIFTLEERLDEFNLEEQSGWGKKSIAH
LLNAIQSRRVITLDRFIFSLGIRFIGQVAAELLADYYVSYDNWYNSMIELSSDNTELVGIDGIGKKVAESLESFFSKEHN
IKMLNDLTACLQILPVSSNSSNSVLNNKIIVFTGKLLAMSRGEAKVRAKTLGAKVSSHLSAKTDYLIAGEKPGSKYKKAV
ELGVEILDEDQWHKVISLGVFK
>Mature_661_residues
TNLEKMREKLQDQINYHNVLYHQKNKPEISDAEYDELKKKLAEIEPEIYATQESVGAPPDERFSKVEHQEPMLSLENAYD
EQGVEKFLSKIKRFLIEDKIEILCEPKIDGLSFSAIYEDGRFVKAATRGDGFVGEDVTHNVATIKGFPKFLQGVQGRLEV
RGEIYISNSDFLKLNENDEFANPRNAAAGSLKQLDANITASRPLRYFAYSLIGGAEKSQSEVLNKLEALGFCVNEHQSLT
SSLDGMLKFYNEVYNCRYNLDYDIDGIVYKVNDLVLQNRLGNTHKAPRSALAYKFSAVYAKTKLNKIFIQVGRTGVLTPV
ADLVPVNIGGVLVSRASLHNQDEIKRKDIREGDIVTIKRAGDVIPQIVEVSRDSRLPNTPEFVFPEVCPECGSKVRQVEG
EAAVRCPEEFACKAQIIEKLKHFVSKDAFDIVGLGDKQIEFFYDLGLIKQIPDIFTLEERLDEFNLEEQSGWGKKSIAHL
LNAIQSRRVITLDRFIFSLGIRFIGQVAAELLADYYVSYDNWYNSMIELSSDNTELVGIDGIGKKVAESLESFFSKEHNI
KMLNDLTACLQILPVSSNSSNSVLNNKIIVFTGKLLAMSRGEAKVRAKTLGAKVSSHLSAKTDYLIAGEKPGSKYKKAVE
LGVEILDEDQWHKVISLGVFK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=679, Percent_Identity=41.8262150220913, Blast_Score=492, Evalue=1e-140,
Organism=Escherichia coli, GI87082305, Length=502, Percent_Identity=23.3067729083665, Blast_Score=119, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 74342; Mature: 74210

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLEKMREKLQDQINYHNVLYHQKNKPEISDAEYDELKKKLAEIEPEIYATQESVGAPP
CCCHHHHHHHHHHHHCHHHEEEECCCCCCCCCCCHHHHHHHHHHCCCHHEECHHHCCCCC
DERFSKVEHQEPMLSLENAYDEQGVEKFLSKIKRFLIEDKIEILCEPKIDGLSFSAIYED
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCEEEEEEECC
GRFVKAATRGDGFVGEDVTHNVATIKGFPKFLQGVQGRLEVRGEIYISNSDFLKLNENDE
CCEEEEECCCCCCCCCCCCCCHHHHHCCHHHHHCCCCEEEEEEEEEECCCCEEEECCCCC
FANPRNAAAGSLKQLDANITASRPLRYFAYSLIGGAEKSQSEVLNKLEALGFCVNEHQSL
CCCCCCHHCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
TSSLDGMLKFYNEVYNCRYNLDYDIDGIVYKVNDLVLQNRLGNTHKAPRSALAYKFSAVY
HHHHHHHHHHHHHHHCEEECCCCCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
AKTKLNKIFIQVGRTGVLTPVADLVPVNIGGVLVSRASLHNQDEIKRKDIREGDIVTIKR
HHHHHHHHHEEECCCCCCCCHHHHHCCCCCHHHHHHHHCCCHHHHHHHCCCCCCEEEEEC
AGDVIPQIVEVSRDSRLPNTPEFVFPEVCPECGSKVRQVEGEAAVRCPEEFACKAQIIEK
CCCHHHHHHHHHHCCCCCCCCCCCCHHHCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHH
LKHFVSKDAFDIVGLGDKQIEFFYDLGLIKQIPDIFTLEERLDEFNLEEQSGWGKKSIAH
HHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHCCCCHHHHHH
LLNAIQSRRVITLDRFIFSLGIRFIGQVAAELLADYYVSYDNWYNSMIELSSDNTELVGI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEE
DGIGKKVAESLESFFSKEHNIKMLNDLTACLQILPVSSNSSNSVLNNKIIVFTGKLLAMS
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCCCCCHHCCEEEEEECCCCEEC
RGEAKVRAKTLGAKVSSHLSAKTDYLIAGEKPGSKYKKAVELGVEILDEDQWHKVISLGV
CCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCC
FK
CC
>Mature Secondary Structure 
TNLEKMREKLQDQINYHNVLYHQKNKPEISDAEYDELKKKLAEIEPEIYATQESVGAPP
CCHHHHHHHHHHHHCHHHEEEECCCCCCCCCCCHHHHHHHHHHCCCHHEECHHHCCCCC
DERFSKVEHQEPMLSLENAYDEQGVEKFLSKIKRFLIEDKIEILCEPKIDGLSFSAIYED
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCEEEEEEECC
GRFVKAATRGDGFVGEDVTHNVATIKGFPKFLQGVQGRLEVRGEIYISNSDFLKLNENDE
CCEEEEECCCCCCCCCCCCCCHHHHHCCHHHHHCCCCEEEEEEEEEECCCCEEEECCCCC
FANPRNAAAGSLKQLDANITASRPLRYFAYSLIGGAEKSQSEVLNKLEALGFCVNEHQSL
CCCCCCHHCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
TSSLDGMLKFYNEVYNCRYNLDYDIDGIVYKVNDLVLQNRLGNTHKAPRSALAYKFSAVY
HHHHHHHHHHHHHHHCEEECCCCCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
AKTKLNKIFIQVGRTGVLTPVADLVPVNIGGVLVSRASLHNQDEIKRKDIREGDIVTIKR
HHHHHHHHHEEECCCCCCCCHHHHHCCCCCHHHHHHHHCCCHHHHHHHCCCCCCEEEEEC
AGDVIPQIVEVSRDSRLPNTPEFVFPEVCPECGSKVRQVEGEAAVRCPEEFACKAQIIEK
CCCHHHHHHHHHHCCCCCCCCCCCCHHHCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHH
LKHFVSKDAFDIVGLGDKQIEFFYDLGLIKQIPDIFTLEERLDEFNLEEQSGWGKKSIAH
HHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHCCCCHHHHHH
LLNAIQSRRVITLDRFIFSLGIRFIGQVAAELLADYYVSYDNWYNSMIELSSDNTELVGI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEE
DGIGKKVAESLESFFSKEHNIKMLNDLTACLQILPVSSNSSNSVLNNKIIVFTGKLLAMS
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCCCCCHHCCEEEEEECCCCEEC
RGEAKVRAKTLGAKVSSHLSAKTDYLIAGEKPGSKYKKAVELGVEILDEDQWHKVISLGV
CCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCC
FK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA