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The map label for this gene is ltrA [H]

Identifier: 225630344

GI number: 225630344

Start: 602696

End: 604009

Strand: Direct

Name: ltrA [H]

Synonym: WRi_005670

Alternate gene names: 225630344

Gene position: 602696-604009 (Clockwise)

Preceding gene: 225630343

Following gene: 225630345

Centisome position: 41.68

GC content: 36.0

Gene sequence:

>1314_bases
ATGCAAGGAAAACTAAACCAGATAGCAGTAAGGGCTAAGCAAGATAAGCGAGTAAAATTTACATCATTAGTTCATTTGAT
TAATGAAGAGAATCTTGCAGAGTGTTATAAGGAACTAAAACGCAACAAGGCTTGTGGTATAGATCGTGTGACAGTGGAAG
CTTATGGAGAAAATCTAGAAGAGAAACTTAAAACACTAGTGGATAGTATGAAGAGAAAACAATATCAACCGCTACCAGTG
AAAAGGGTATACATACCCAAAGCCGGGAGCAAAGAGAAGCGCGGTCTCGGAATACCATCAACGGAAGATAAGTTGGTACA
GGTAATGCTAAAGAAGATATTAGAAAATATTTATGAAGCAAACTTTATGGACAGCTCGTATGGATTTCGACCAGGAAGAA
ATTGTCATCAGGCGATAAACGCTCTAGATAAAGCAGTTATGCACAAACCAATTAACTATATTGTAGAAGTGGATATCAAG
AAGTTCTTTGATAATGTTCAACATAAATGGCTAATGAATTGTCTAAGGGAACGAATAGCTGACCCAAATTTATTGTGGTT
AATAAAACGATTTCTAAAGGCAGGAATAGTCGAAGTTGGATGTTATAAAGCAACCGATCAAGGCACACCACAAGGTGGTA
TAGTAAGCCCTGTATTAGCTAATATATACTTACACTATGTGCTGGACTTATGGTTTGAAAAGAAATTTAAGCCAAAAGCC
AGAGGATATTTACAGCTAATAAGGTTTTGCGACGATTTTGTAGTTGGCTGCGAGAGGGAAGAAGACGCAAAAGAATTTCT
AGAATTACTGAAACAAAGACTAAGTAAATTTGGGTTGGAAATAGCTGAAAATAAAACAAAAATAGTAAAGTTTGGTAAGA
AAGAATGGTATCAAGCAGAAAGAGAGAAACGTAGGACGGCTAGCTTCAACTTTCTAGGATTTACACATTATTGTGGAAAA
AGTCGTAATGGTAAACTTATGATGAAGCAGAAAACTTCAAAAATAAGCCTAGCCAGAAAGATTAAAGAAATCAAAGAATG
GTTGAAGATGGTACGAAGTCGTATCTGTCTCAAAGATTGGTGGCAGAAACTTAAAGCCAAACTAACAGGACACTATAGCT
ACTTCGGAGTTAGCGGAAATTATCGGTGTTTGATTCAATTCTATCGACCGGTAACAAAGTTAGCATTTAAGTGGATAAAC
CGACGTAGTCAAAAGAAAAGTATGGATTGGGAACAATTTATACACTATTTAAAAGTAAATCCATTACCAAAACCAAAGGT
ATATGTTTCTTTATACACAGGAGCACTAGTGTGA

Upstream 100 bases:

>100_bases
GTACTCGGAGTGTGGGAAAGCCACATACAAGGGGAAGCGGTTAGCAATATAGTGACTGGTATAGGGATTGCTATACCAAT
ACAACAGAGATTGGAGGAGT

Downstream 100 bases:

>100_bases
ACGCTGTATTGTGGAGCCGTGTGCGGGAAATCCGCAAGCACGGTTCTTTTGGGGAGACTATGGTTATGAGACCAAATCGC
TGGTTTCAAGGTCATGAGTC

Product: RNA-directed DNA polymerase (Reverse transcriptase)

Products: NA

Alternate protein names: Reverse-transcriptase; RNA maturase; DNA endonuclease [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MQGKLNQIAVRAKQDKRVKFTSLVHLINEENLAECYKELKRNKACGIDRVTVEAYGENLEEKLKTLVDSMKRKQYQPLPV
KRVYIPKAGSKEKRGLGIPSTEDKLVQVMLKKILENIYEANFMDSSYGFRPGRNCHQAINALDKAVMHKPINYIVEVDIK
KFFDNVQHKWLMNCLRERIADPNLLWLIKRFLKAGIVEVGCYKATDQGTPQGGIVSPVLANIYLHYVLDLWFEKKFKPKA
RGYLQLIRFCDDFVVGCEREEDAKEFLELLKQRLSKFGLEIAENKTKIVKFGKKEWYQAEREKRRTASFNFLGFTHYCGK
SRNGKLMMKQKTSKISLARKIKEIKEWLKMVRSRICLKDWWQKLKAKLTGHYSYFGVSGNYRCLIQFYRPVTKLAFKWIN
RRSQKKSMDWEQFIHYLKVNPLPKPKVYVSLYTGALV

Sequences:

>Translated_437_residues
MQGKLNQIAVRAKQDKRVKFTSLVHLINEENLAECYKELKRNKACGIDRVTVEAYGENLEEKLKTLVDSMKRKQYQPLPV
KRVYIPKAGSKEKRGLGIPSTEDKLVQVMLKKILENIYEANFMDSSYGFRPGRNCHQAINALDKAVMHKPINYIVEVDIK
KFFDNVQHKWLMNCLRERIADPNLLWLIKRFLKAGIVEVGCYKATDQGTPQGGIVSPVLANIYLHYVLDLWFEKKFKPKA
RGYLQLIRFCDDFVVGCEREEDAKEFLELLKQRLSKFGLEIAENKTKIVKFGKKEWYQAEREKRRTASFNFLGFTHYCGK
SRNGKLMMKQKTSKISLARKIKEIKEWLKMVRSRICLKDWWQKLKAKLTGHYSYFGVSGNYRCLIQFYRPVTKLAFKWIN
RRSQKKSMDWEQFIHYLKVNPLPKPKVYVSLYTGALV
>Mature_437_residues
MQGKLNQIAVRAKQDKRVKFTSLVHLINEENLAECYKELKRNKACGIDRVTVEAYGENLEEKLKTLVDSMKRKQYQPLPV
KRVYIPKAGSKEKRGLGIPSTEDKLVQVMLKKILENIYEANFMDSSYGFRPGRNCHQAINALDKAVMHKPINYIVEVDIK
KFFDNVQHKWLMNCLRERIADPNLLWLIKRFLKAGIVEVGCYKATDQGTPQGGIVSPVLANIYLHYVLDLWFEKKFKPKA
RGYLQLIRFCDDFVVGCEREEDAKEFLELLKQRLSKFGLEIAENKTKIVKFGKKEWYQAEREKRRTASFNFLGFTHYCGK
SRNGKLMMKQKTSKISLARKIKEIKEWLKMVRSRICLKDWWQKLKAKLTGHYSYFGVSGNYRCLIQFYRPVTKLAFKWIN
RRSQKKSMDWEQFIHYLKVNPLPKPKVYVSLYTGALV

Specific function: Multifunctional protein that promotes group II intron splicing and mobility by acting both on RNA and DNA. It has three activities:reverse transcriptase (RT) for intron duplication, maturase to promote splicing, and DNA endonuclease for site- specific cle

COG id: COG3344

COG function: function code L; Retron-type reverse transcriptase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 reverse transcriptase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6226521, Length=202, Percent_Identity=35.6435643564356, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6226520, Length=310, Percent_Identity=26.1290322580645, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR002866
- InterPro:   IPR000477 [H]

Pfam domain/function: PF01348 Intron_maturas2; PF00078 RVT_1 [H]

EC number: =2.7.7.49 [H]

Molecular weight: Translated: 51337; Mature: 51337

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS00559 MOLYBDOPTERIN_EUK ; PS50878 RT_POL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCEEEEEECCCCC
>Mature Secondary Structure
MQGKLNQIAVRAKQDKRVKFTSLVHLINEENLAECYKELKRNKACGIDRVTVEAYGENLE
CCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCHH
EKLKTLVDSMKRKQYQPLPVKRVYIPKAGSKEKRGLGIPSTEDKLVQVMLKKILENIYEA
HHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
NFMDSSYGFRPGRNCHQAINALDKAVMHKPINYIVEVDIKKFFDNVQHKWLMNCLRERIA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DPNLLWLIKRFLKAGIVEVGCYKATDQGTPQGGIVSPVLANIYLHYVLDLWFEKKFKPKA
CCHHHHHHHHHHHCCCHHHCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
RGYLQLIRFCDDFVVGCEREEDAKEFLELLKQRLSKFGLEIAENKTKIVKFGKKEWYQAE
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHCCHHHHHHHH
REKRRTASFNFLGFTHYCGKSRNGKLMMKQKTSKISLARKIKEIKEWLKMVRSRICLKDW
HHHHHHHCCCEEEHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WQKLKAKLTGHYSYFGVSGNYRCLIQFYRPVTKLAFKWINRRSQKKSMDWEQFIHYLKVN
HHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
PLPKPKVYVSLYTGALV
CCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8655550; 11157264; 10488339; 9353259; 10413481; 11731786 [H]