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The map label for this gene is cisA [H]

Identifier: 225630319

GI number: 225630319

Start: 581150

End: 582643

Strand: Reverse

Name: cisA [H]

Synonym: WRi_005400

Alternate gene names: 225630319

Gene position: 582643-581150 (Counterclockwise)

Preceding gene: 225630321

Following gene: 225630316

Centisome position: 40.3

GC content: 35.68

Gene sequence:

>1494_bases
ATGCTGAAAGAGGTAAGATGCGCGATATATACGAGAAAATCAAATGAAGACGGGCTAGAACAAAAGTTTAATAGTTTAGA
TGCACAGCGAGTAGTATGTGAGAAGTATATAAAAAGCCGAGAAGGGTGGGTAGCATTGGCAAAAAAGTACGATGATGGAG
GGTTCTCAGGGAGTAATTTGAATAGACCAGCGATAAAGGAATTGTTTGAAGATGTAAAGGTAGGAGAAGTCGACTGTGTA
GTAGTATATACGCTAGATAGGTTATCAAGAGAAACAAAGGACTGCATAGAAGTAACATCATTTTTTAGAAGGCACCGGAT
AAGTTTTGTAGCAGTAACACAAATATTCGACAATAATACGCCAATGGGAAAATTTGTACAAACAGTGCTATCAGGGGCGG
CACAACTAGAAAGAGAAATGATAGTAGAGAGAGTAAAAAATAAAATAGCAACATCGAAAGAACAAGGGTTATGGATGGGT
GGAACTTTACCGCTTGGGTATGATGTAAAAGATAAAGAATTAATAATAAATGAAAAAGAAGCAAAGACAGTGAAGCATAT
ATTTGAAAGGTATGTAGAGCTGAAGTCAATGGCAGAGTTGGCAAGGGAGTTGAACAGAGAAGGATATCGAACAAAAGCAA
AATCAGATATTTTTAAAAAAGCAACAGTGAGAAGAATAATAACAAATCCAATATACATGGGAAAGATAAGACATTATGAC
AAACAGTATGAGGGAAAGCATGAGGCGATAATAGAAGAAGAAAAATGGCAAAAAGCGCAGGAATTGATAAGGAATCAACC
ATATAAAAAAGCAAAATATGAGGAAGCTTTGCTTAGGGGAATAATTAAGTGCAAGAGCTGTGATGTAAATATGACGCTGA
CGTATGCAAAAAAGGAAAATAAAAGGTATCGATACTACGTATGCAATAATCATTTAAGGGGAAAAAATTGTGAATCAGTG
AATCGAACAATAGTAGCTGGAGAAGTGGAAAAAGAAGTAATGAGGAAGACAGAGCAGCTATATGAAAATTGGAAAGAAAA
GACCGAAGAAGAGTGGAAAAATTTAAGTTTTGGAAAGCAGAAAGAAGTAGTGAAAAAGTTAATAAAAGGAGTAATGGTAA
AAGGAGATGGAATAGAAGTGAGTTCAGAGGATAAGATAGAATTTATACCAATAAAGAAGAAAGGAAACAAATGTGTAGTA
ATGGAACCTGAAGGAAAAACGAACAATGCGTTACTGAAAGCAGTGGTAAGAGCCCATCTGTGGAAACGGCAACTAGAAGA
GGGAAAATATGGAAGTATAAAAGAGCTGAGTGCCAAAATTAATATAGGTGCAAGACGTATACAGCAGATTTTAAGGTTGA
ATTATTTAGCACCAAAGATAAAAGAAGACATAGTAAATGGGAGGCAGCCAAGGGGTTTGAAGTTAGTTGATTTAAAAGAA
ATACCGATGCTGTGGAGTGAGCAAAGGGAGAAATTCTATGGTCTTGATTTATAA

Upstream 100 bases:

>100_bases
AAGAAGAGTTTTATACGTCATTATCAGCAGTAGCCGGCAAAATAATGGGAATGAGTTATAATGGACCGCTCCTATTTGGA
ATGAGAGATAAGGAGAAAGA

Downstream 100 bases:

>100_bases
AAGCAACAGAAAAGGGCCACACAAAAAGATGTTGAAATTGATATAGAAAAGTAAGTCAATGTTACAGAATCAATTATGCG
AAAATATCAATATAAATAAT

Product: site-specific recombinase, resolvase family

Products: NA

Alternate protein names: Stage IV sporulation protein CA [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MLKEVRCAIYTRKSNEDGLEQKFNSLDAQRVVCEKYIKSREGWVALAKKYDDGGFSGSNLNRPAIKELFEDVKVGEVDCV
VVYTLDRLSRETKDCIEVTSFFRRHRISFVAVTQIFDNNTPMGKFVQTVLSGAAQLEREMIVERVKNKIATSKEQGLWMG
GTLPLGYDVKDKELIINEKEAKTVKHIFERYVELKSMAELARELNREGYRTKAKSDIFKKATVRRIITNPIYMGKIRHYD
KQYEGKHEAIIEEEKWQKAQELIRNQPYKKAKYEEALLRGIIKCKSCDVNMTLTYAKKENKRYRYYVCNNHLRGKNCESV
NRTIVAGEVEKEVMRKTEQLYENWKEKTEEEWKNLSFGKQKEVVKKLIKGVMVKGDGIEVSSEDKIEFIPIKKKGNKCVV
MEPEGKTNNALLKAVVRAHLWKRQLEEGKYGSIKELSAKINIGARRIQQILRLNYLAPKIKEDIVNGRQPRGLKLVDLKE
IPMLWSEQREKFYGLDL

Sequences:

>Translated_497_residues
MLKEVRCAIYTRKSNEDGLEQKFNSLDAQRVVCEKYIKSREGWVALAKKYDDGGFSGSNLNRPAIKELFEDVKVGEVDCV
VVYTLDRLSRETKDCIEVTSFFRRHRISFVAVTQIFDNNTPMGKFVQTVLSGAAQLEREMIVERVKNKIATSKEQGLWMG
GTLPLGYDVKDKELIINEKEAKTVKHIFERYVELKSMAELARELNREGYRTKAKSDIFKKATVRRIITNPIYMGKIRHYD
KQYEGKHEAIIEEEKWQKAQELIRNQPYKKAKYEEALLRGIIKCKSCDVNMTLTYAKKENKRYRYYVCNNHLRGKNCESV
NRTIVAGEVEKEVMRKTEQLYENWKEKTEEEWKNLSFGKQKEVVKKLIKGVMVKGDGIEVSSEDKIEFIPIKKKGNKCVV
MEPEGKTNNALLKAVVRAHLWKRQLEEGKYGSIKELSAKINIGARRIQQILRLNYLAPKIKEDIVNGRQPRGLKLVDLKE
IPMLWSEQREKFYGLDL
>Mature_497_residues
MLKEVRCAIYTRKSNEDGLEQKFNSLDAQRVVCEKYIKSREGWVALAKKYDDGGFSGSNLNRPAIKELFEDVKVGEVDCV
VVYTLDRLSRETKDCIEVTSFFRRHRISFVAVTQIFDNNTPMGKFVQTVLSGAAQLEREMIVERVKNKIATSKEQGLWMG
GTLPLGYDVKDKELIINEKEAKTVKHIFERYVELKSMAELARELNREGYRTKAKSDIFKKATVRRIITNPIYMGKIRHYD
KQYEGKHEAIIEEEKWQKAQELIRNQPYKKAKYEEALLRGIIKCKSCDVNMTLTYAKKENKRYRYYVCNNHLRGKNCESV
NRTIVAGEVEKEVMRKTEQLYENWKEKTEEEWKNLSFGKQKEVVKKLIKGVMVKGDGIEVSSEDKIEFIPIKKKGNKCVV
MEPEGKTNNALLKAVVRAHLWKRQLEEGKYGSIKELSAKINIGARRIQQILRLNYLAPKIKEDIVNGRQPRGLKLVDLKE
IPMLWSEQREKFYGLDL

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=110, Percent_Identity=37.2727272727273, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 57859; Mature: 57859

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEVRCAIYTRKSNEDGLEQKFNSLDAQRVVCEKYIKSREGWVALAKKYDDGGFSGSNL
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
NRPAIKELFEDVKVGEVDCVVVYTLDRLSRETKDCIEVTSFFRRHRISFVAVTQIFDNNT
CCHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHCCCC
PMGKFVQTVLSGAAQLEREMIVERVKNKIATSKEQGLWMGGTLPLGYDVKDKELIINEKE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCEEECCCCCCCCCCCCCCEEECCHH
AKTVKHIFERYVELKSMAELARELNREGYRTKAKSDIFKKATVRRIITNPIYMGKIRHYD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KQYEGKHEAIIEEEKWQKAQELIRNQPYKKAKYEEALLRGIIKCKSCDVNMTLTYAKKEN
HHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
KRYRYYVCNNHLRGKNCESVNRTIVAGEVEKEVMRKTEQLYENWKEKTEEEWKNLSFGKQ
CEEEEEEECCCCCCCCCCHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
KEVVKKLIKGVMVKGDGIEVSSEDKIEFIPIKKKGNKCVVMEPEGKTNNALLKAVVRAHL
HHHHHHHHHHHEECCCCEEECCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
WKRQLEEGKYGSIKELSAKINIGARRIQQILRLNYLAPKIKEDIVNGRQPRGLKLVDLKE
HHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCCEEEECHHH
IPMLWSEQREKFYGLDL
CCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKEVRCAIYTRKSNEDGLEQKFNSLDAQRVVCEKYIKSREGWVALAKKYDDGGFSGSNL
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
NRPAIKELFEDVKVGEVDCVVVYTLDRLSRETKDCIEVTSFFRRHRISFVAVTQIFDNNT
CCHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHCCCC
PMGKFVQTVLSGAAQLEREMIVERVKNKIATSKEQGLWMGGTLPLGYDVKDKELIINEKE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCEEECCCCCCCCCCCCCCEEECCHH
AKTVKHIFERYVELKSMAELARELNREGYRTKAKSDIFKKATVRRIITNPIYMGKIRHYD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KQYEGKHEAIIEEEKWQKAQELIRNQPYKKAKYEEALLRGIIKCKSCDVNMTLTYAKKEN
HHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
KRYRYYVCNNHLRGKNCESVNRTIVAGEVEKEVMRKTEQLYENWKEKTEEEWKNLSFGKQ
CEEEEEEECCCCCCCCCCHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
KEVVKKLIKGVMVKGDGIEVSSEDKIEFIPIKKKGNKCVVMEPEGKTNNALLKAVVRAHL
HHHHHHHHHHHEECCCCEEECCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
WKRQLEEGKYGSIKELSAKINIGARRIQQILRLNYLAPKIKEDIVNGRQPRGLKLVDLKE
HHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCCEEEECHHH
IPMLWSEQREKFYGLDL
CCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377 [H]