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The map label for this gene is recJ [H]

Identifier: 225630248

GI number: 225630248

Start: 498248

End: 499987

Strand: Reverse

Name: recJ [H]

Synonym: WRi_004620

Alternate gene names: 225630248

Gene position: 499987-498248 (Counterclockwise)

Preceding gene: 225630249

Following gene: 225630246

Centisome position: 34.58

GC content: 36.67

Gene sequence:

>1740_bases
ATGCTACTTGAAATTGAAAAGCGCAGCATAACAAATGCATTGTGGAAGTTGCAAGAAGCAGATCAAAGGCAAATCTTAAC
TCTTACACAGAGATTTGAGTTACCAGAAATATTAGCAAGAATATTGGTTACTCGTGGTGTTAACATAGAAAATGCAAGCG
ACTTCTTATATCCACTAATCAGATCGCTATTACCAGATCCCTTTCACCTTCTTGATATGGATAAAGCTGTTTCTCGTATA
ATACGGGCAATAAAAAATGATGAAAACCTTGCAATATTTGGTGACTATGATGTTGATGGTGCAACGTCGTCAGCATTGAT
TAACAGGTACCTGAGAGCAATCGATATACACTCTATAATCTACATTCCAGATCGTGTTGATGAGGGTTATGGATTAAGCA
CAGATGCTTTATTACAACTTAAAAAGAATGGAATTGATTTATGTATTTCCGTTGATTGTGGTACGCTTGCATATCAACCA
ATAGAAGATGCAAAGGCTTTTGGTCTTGATATTATAGTTGTTGACCATCACCTTGGTACGGAAAAATTACCAAGCGCCGT
AGCAATTGTGAATCCAAACCGCCTTGATGAGAGCTCTCCTTATAATAACCTAGCAGCAGTTGGAGTGTCATTTCTGCTAA
TTGTTGCTCTTAATAAAAGCCTACGTGAGCAAGGATTTTTTACCAACAAAAAAGAACCAGATTTATTTGATCTGTTGGAT
TTGGTTGCCCTGGGAACTGTCTGTGATGTTATGCAGATCACAGGCCTCAATAGAGCACTTGTCTCACAAGGATTAAAAGT
TATGTCAACAAGAAAAAACGTTGGCTTGCGTGTTTTATTTGATGCTTTGGGAATCCTTGAAAAACCAAGTGTTTCTCGAT
TAGGGTTTAGTATTGGGCCATGCATAAATGCTGGAGGAAGAATTGGAGAAGCATCGCTTGGTGCAAGGTTGCTTTCCACC
GATGATGATGAAGAGGCACATTCAATCGCGCTAAAATTAATAGATTTGAATAATGCAAGAAAAGTGTTAGAAAATGAGGC
TCTCTTGGAAGCTACAGCACAAGCGGAAAAGTCTGCCCAATCAGGTGCGAATTTTATAATGATAAGTGGCAATTGGCACC
AGGGAATAATTGGTATAATTGCATCAAGGCTAAAGGAGCAGTTTCACTTGCCAACAGTAGTGATATCTTTAAATAATGGA
ATAGGAAAAGCAAGCTGTAGATCAATTTTGGGAGTTGATATCGGTGCTGCAGTCCTTTCCGCAAAGTTTATAAATTTGAT
TATTGAAGGTGGTGGCCATAGTATGGCGGCAGGATTTTCAATTAAAGAAGACAAAATAAATGATTTGCATGATTTTTTTA
CTGAAAGATTTTCAAATTCTATAAATGAGAAAACTTTAAAAGCTGATAGTATAGTAACTGCTAAAGCAATAAATTTGTCT
TTATGGAATCAATTGCAACGCTTGGGACCATTTGGAGTTGGAAATCCTGAACCAAGGTTTATTATCCAAGGAGCAAAGAT
AAGAAAGCCTGAAGTTATAGGAGTTGACCATATAAAATGTTTTATTGCTGATGATAATGTTATGGTAAAAGCTATTGCAT
TTCGTTCTGCAAATACTCATCTTGGCTCTGCTATCATGCAGGGTAATGTTAAAGCTATTTTGGGTAAAATCTCTATGAAT
TACTGGAATGGCAATGAATTTGTACAATTTTTAATAGAAGATGTATTGACCATTAGTTGA

Upstream 100 bases:

>100_bases
TATCTTTTTTCTACTTAGTTTAGGTTATGCAGAAGTCTAATACAATTATAGAACAAGCTTAAAAATTCTGGTAAAGTAGT
TTTATTTACAAAGAAAGAAT

Downstream 100 bases:

>100_bases
GTGTATCTGTTCAGCAGAGTGGCAAAATAGGTAGACAAGATAAACCAAAAAATCGAGAAAAAGTGAGTTTACAACAACTG
GTGTCATGAAATTATCTGAC

Product: single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MLLEIEKRSITNALWKLQEADQRQILTLTQRFELPEILARILVTRGVNIENASDFLYPLIRSLLPDPFHLLDMDKAVSRI
IRAIKNDENLAIFGDYDVDGATSSALINRYLRAIDIHSIIYIPDRVDEGYGLSTDALLQLKKNGIDLCISVDCGTLAYQP
IEDAKAFGLDIIVVDHHLGTEKLPSAVAIVNPNRLDESSPYNNLAAVGVSFLLIVALNKSLREQGFFTNKKEPDLFDLLD
LVALGTVCDVMQITGLNRALVSQGLKVMSTRKNVGLRVLFDALGILEKPSVSRLGFSIGPCINAGGRIGEASLGARLLST
DDDEEAHSIALKLIDLNNARKVLENEALLEATAQAEKSAQSGANFIMISGNWHQGIIGIIASRLKEQFHLPTVVISLNNG
IGKASCRSILGVDIGAAVLSAKFINLIIEGGGHSMAAGFSIKEDKINDLHDFFTERFSNSINEKTLKADSIVTAKAINLS
LWNQLQRLGPFGVGNPEPRFIIQGAKIRKPEVIGVDHIKCFIADDNVMVKAIAFRSANTHLGSAIMQGNVKAILGKISMN
YWNGNEFVQFLIEDVLTIS

Sequences:

>Translated_579_residues
MLLEIEKRSITNALWKLQEADQRQILTLTQRFELPEILARILVTRGVNIENASDFLYPLIRSLLPDPFHLLDMDKAVSRI
IRAIKNDENLAIFGDYDVDGATSSALINRYLRAIDIHSIIYIPDRVDEGYGLSTDALLQLKKNGIDLCISVDCGTLAYQP
IEDAKAFGLDIIVVDHHLGTEKLPSAVAIVNPNRLDESSPYNNLAAVGVSFLLIVALNKSLREQGFFTNKKEPDLFDLLD
LVALGTVCDVMQITGLNRALVSQGLKVMSTRKNVGLRVLFDALGILEKPSVSRLGFSIGPCINAGGRIGEASLGARLLST
DDDEEAHSIALKLIDLNNARKVLENEALLEATAQAEKSAQSGANFIMISGNWHQGIIGIIASRLKEQFHLPTVVISLNNG
IGKASCRSILGVDIGAAVLSAKFINLIIEGGGHSMAAGFSIKEDKINDLHDFFTERFSNSINEKTLKADSIVTAKAINLS
LWNQLQRLGPFGVGNPEPRFIIQGAKIRKPEVIGVDHIKCFIADDNVMVKAIAFRSANTHLGSAIMQGNVKAILGKISMN
YWNGNEFVQFLIEDVLTIS
>Mature_579_residues
MLLEIEKRSITNALWKLQEADQRQILTLTQRFELPEILARILVTRGVNIENASDFLYPLIRSLLPDPFHLLDMDKAVSRI
IRAIKNDENLAIFGDYDVDGATSSALINRYLRAIDIHSIIYIPDRVDEGYGLSTDALLQLKKNGIDLCISVDCGTLAYQP
IEDAKAFGLDIIVVDHHLGTEKLPSAVAIVNPNRLDESSPYNNLAAVGVSFLLIVALNKSLREQGFFTNKKEPDLFDLLD
LVALGTVCDVMQITGLNRALVSQGLKVMSTRKNVGLRVLFDALGILEKPSVSRLGFSIGPCINAGGRIGEASLGARLLST
DDDEEAHSIALKLIDLNNARKVLENEALLEATAQAEKSAQSGANFIMISGNWHQGIIGIIASRLKEQFHLPTVVISLNNG
IGKASCRSILGVDIGAAVLSAKFINLIIEGGGHSMAAGFSIKEDKINDLHDFFTERFSNSINEKTLKADSIVTAKAINLS
LWNQLQRLGPFGVGNPEPRFIIQGAKIRKPEVIGVDHIKCFIADDNVMVKAIAFRSANTHLGSAIMQGNVKAILGKISMN
YWNGNEFVQFLIEDVLTIS

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=567, Percent_Identity=33.8624338624339, Blast_Score=284, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 63287; Mature: 63287

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEIEKRSITNALWKLQEADQRQILTLTQRFELPEILARILVTRGVNIENASDFLYPLI
CEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHH
RSLLPDPFHLLDMDKAVSRIIRAIKNDENLAIFGDYDVDGATSSALINRYLRAIDIHSII
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCEEE
YIPDRVDEGYGLSTDALLQLKKNGIDLCISVDCGTLAYQPIEDAKAFGLDIIVVDHHLGT
EECCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCEECCCCHHHHHCCEEEEEEECCCCC
EKLPSAVAIVNPNRLDESSPYNNLAAVGVSFLLIVALNKSLREQGFFTNKKEPDLFDLLD
HHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LVALGTVCDVMQITGLNRALVSQGLKVMSTRKNVGLRVLFDALGILEKPSVSRLGFSIGP
HHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHCCCCHHHHHHHHCCCCCCCHHHCCCCCCC
CINAGGRIGEASLGARLLSTDDDEEAHSIALKLIDLNNARKVLENEALLEATAQAEKSAQ
CCCCCCCCCCHHHCCEEECCCCCCHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
SGANFIMISGNWHQGIIGIIASRLKEQFHLPTVVISLNNGIGKASCRSILGVDIGAAVLS
CCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCHHHHHHHH
AKFINLIIEGGGHSMAAGFSIKEDKINDLHDFFTERFSNSINEKTLKADSIVTAKAINLS
HHHHEEEEECCCCCEECCCEECHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEHHHHHHH
LWNQLQRLGPFGVGNPEPRFIIQGAKIRKPEVIGVDHIKCFIADDNVMVKAIAFRSANTH
HHHHHHHHCCCCCCCCCCCEEEECCCCCCCCEECCCEEEEEEECCCEEEEEEEECCCCCH
LGSAIMQGNVKAILGKISMNYWNGNEFVQFLIEDVLTIS
HHHHHHHCCHHHHHHHHEEEEECHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLEIEKRSITNALWKLQEADQRQILTLTQRFELPEILARILVTRGVNIENASDFLYPLI
CEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHH
RSLLPDPFHLLDMDKAVSRIIRAIKNDENLAIFGDYDVDGATSSALINRYLRAIDIHSII
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCEEE
YIPDRVDEGYGLSTDALLQLKKNGIDLCISVDCGTLAYQPIEDAKAFGLDIIVVDHHLGT
EECCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCEECCCCHHHHHCCEEEEEEECCCCC
EKLPSAVAIVNPNRLDESSPYNNLAAVGVSFLLIVALNKSLREQGFFTNKKEPDLFDLLD
HHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LVALGTVCDVMQITGLNRALVSQGLKVMSTRKNVGLRVLFDALGILEKPSVSRLGFSIGP
HHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHCCCCHHHHHHHHCCCCCCCHHHCCCCCCC
CINAGGRIGEASLGARLLSTDDDEEAHSIALKLIDLNNARKVLENEALLEATAQAEKSAQ
CCCCCCCCCCHHHCCEEECCCCCCHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
SGANFIMISGNWHQGIIGIIASRLKEQFHLPTVVISLNNGIGKASCRSILGVDIGAAVLS
CCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCHHHHHHHH
AKFINLIIEGGGHSMAAGFSIKEDKINDLHDFFTERFSNSINEKTLKADSIVTAKAINLS
HHHHEEEEECCCCCEECCCEECHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEHHHHHHH
LWNQLQRLGPFGVGNPEPRFIIQGAKIRKPEVIGVDHIKCFIADDNVMVKAIAFRSANTH
HHHHHHHHCCCCCCCCCCCEEEECCCCCCCCEECCCEEEEEEECCCEEEEEEEECCCCCH
LGSAIMQGNVKAILGKISMNYWNGNEFVQFLIEDVLTIS
HHHHHHHCCHHHHHHHHEEEEECHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]