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The map label for this gene is ygfZ [C]

Identifier: 225630247

GI number: 225630247

Start: 496521

End: 497327

Strand: Direct

Name: ygfZ [C]

Synonym: WRi_004610

Alternate gene names: 225630247

Gene position: 496521-497327 (Clockwise)

Preceding gene: 225630243

Following gene: 225630252

Centisome position: 34.34

GC content: 36.31

Gene sequence:

>807_bases
ATGAGTTATATACCATTTTTAAGTCGTGGTGTGATAGTGCTATATGGGCCTGACACTAGAGATTTTCTGCAGGGTATTAT
AACCAATGATATTAATAAGTTGGATAGCCAAAAAGCCATTTACTCTTTATTGCTTAGCCCTCAGGGAAAATATCTATATG
ATTTTTTTCTTATTGAATATGGTAAATACACCCTCTTAGAGTGCGAAAACATGCACTTGCAGCAAATAATCGAGAAACTG
GATTTGCTTAAAACTTATTTGAAAGTGAAAATTAAAGACGTTAGCGCACTATATAAGGTTGGAGTTTTGTTTAATACTAA
GTTGGCGGAGTGTAGTAGTGAATCACAAGTTATTTTTCAAGATCCAAGGCACAAGCTGCTAGGAATGAGGATCATACATA
AAGATGAAATAAAAGAGCCGGTTGGGGATTTTACTCAGTATGAAAAAGTTAGAATTCAAAATCTCGTTCCAGATGGAGCG
AAAGATATGGTGCAGAATTCATCGTTTCCGCTGCAATTTTTAATCGATAAGGTAAACGGTATAAGCTTTAATAAGGGGTG
TTATATAGGTCAGGAAGTTGTAAATCGAATGAGCAGACAAGAAATATTCAGAAGAAAACTTTACCTTGTTGAGGGGGATA
ACGCACTTCCAGATATTGGCACTAAAGTGACAAATGAAAACAATGAGGAAATAGGGGAACTGCGCTCTAGTGTAGACAAC
ATTGGACTTGCATTGCTTAATACTGGAAAGAGCTATGCAAATTTATATGCTGGTGGGGTCAAGTGCGTTAAGACGTTGAA
GTCTTAA

Upstream 100 bases:

>100_bases
TGTAAAATATGCACGCCATTATAAAATATGCACGCCAAAAGCAGTTTCTAAAAGAGCCTAATGTTATATAATAAAATTTA
GATTTATTTATAGAGATTTC

Downstream 100 bases:

>100_bases
GGTAGCCCATATAGAAACGAAAAACTTGCTTGACACCCTTCGCCAGCGCCCTTATCATGATATTGAAGGTATTCAGTTAT
CTTCATCTGTGCAGATTAAA

Product: aminomethyl transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MSYIPFLSRGVIVLYGPDTRDFLQGIITNDINKLDSQKAIYSLLLSPQGKYLYDFFLIEYGKYTLLECENMHLQQIIEKL
DLLKTYLKVKIKDVSALYKVGVLFNTKLAECSSESQVIFQDPRHKLLGMRIIHKDEIKEPVGDFTQYEKVRIQNLVPDGA
KDMVQNSSFPLQFLIDKVNGISFNKGCYIGQEVVNRMSRQEIFRRKLYLVEGDNALPDIGTKVTNENNEEIGELRSSVDN
IGLALLNTGKSYANLYAGGVKCVKTLKS

Sequences:

>Translated_268_residues
MSYIPFLSRGVIVLYGPDTRDFLQGIITNDINKLDSQKAIYSLLLSPQGKYLYDFFLIEYGKYTLLECENMHLQQIIEKL
DLLKTYLKVKIKDVSALYKVGVLFNTKLAECSSESQVIFQDPRHKLLGMRIIHKDEIKEPVGDFTQYEKVRIQNLVPDGA
KDMVQNSSFPLQFLIDKVNGISFNKGCYIGQEVVNRMSRQEIFRRKLYLVEGDNALPDIGTKVTNENNEEIGELRSSVDN
IGLALLNTGKSYANLYAGGVKCVKTLKS
>Mature_267_residues
SYIPFLSRGVIVLYGPDTRDFLQGIITNDINKLDSQKAIYSLLLSPQGKYLYDFFLIEYGKYTLLECENMHLQQIIEKLD
LLKTYLKVKIKDVSALYKVGVLFNTKLAECSSESQVIFQDPRHKLLGMRIIHKDEIKEPVGDFTQYEKVRIQNLVPDGAK
DMVQNSSFPLQFLIDKVNGISFNKGCYIGQEVVNRMSRQEIFRRKLYLVEGDNALPDIGTKVTNENNEEIGELRSSVDNI
GLALLNTGKSYANLYAGGVKCVKTLKS

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Homo sapiens, GI58197556, Length=278, Percent_Identity=28.4172661870504, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71985951, Length=261, Percent_Identity=30.6513409961686, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24645123, Length=271, Percent_Identity=26.1992619926199, Blast_Score=95, Evalue=6e-20,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 30467; Mature: 30336

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYIPFLSRGVIVLYGPDTRDFLQGIITNDINKLDSQKAIYSLLLSPQGKYLYDFFLIEY
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
GKYTLLECENMHLQQIIEKLDLLKTYLKVKIKDVSALYKVGVLFNTKLAECSSESQVIFQ
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEE
DPRHKLLGMRIIHKDEIKEPVGDFTQYEKVRIQNLVPDGAKDMVQNSSFPLQFLIDKVNG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHCC
ISFNKGCYIGQEVVNRMSRQEIFRRKLYLVEGDNALPDIGTKVTNENNEEIGELRSSVDN
CCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
IGLALLNTGKSYANLYAGGVKCVKTLKS
HCEEEEECCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SYIPFLSRGVIVLYGPDTRDFLQGIITNDINKLDSQKAIYSLLLSPQGKYLYDFFLIEY
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
GKYTLLECENMHLQQIIEKLDLLKTYLKVKIKDVSALYKVGVLFNTKLAECSSESQVIFQ
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEE
DPRHKLLGMRIIHKDEIKEPVGDFTQYEKVRIQNLVPDGAKDMVQNSSFPLQFLIDKVNG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHCC
ISFNKGCYIGQEVVNRMSRQEIFRRKLYLVEGDNALPDIGTKVTNENNEEIGELRSSVDN
CCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
IGLALLNTGKSYANLYAGGVKCVKTLKS
HCEEEEECCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA