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The map label for this gene is purA [H]

Identifier: 225630237

GI number: 225630237

Start: 479852

End: 481129

Strand: Direct

Name: purA [H]

Synonym: WRi_004490

Alternate gene names: 225630237

Gene position: 479852-481129 (Clockwise)

Preceding gene: 225630236

Following gene: 225630238

Centisome position: 33.19

GC content: 35.99

Gene sequence:

>1278_bases
ATGAATAATATTGTAATTGTTGGTCTGCAATGGGGTGACGAAGGCAAGGGTAAAATAGTAGATTATCTTTCTGAGAATGC
AGATGTAGTTGTGAGATTTCAGGGAGGAAATAATGCAGGGCACACTATAGTAGTAGATGATGAGGTTTATAAATTAAATT
TACTGCCCTCTGCTGTTTTGAGGCCAGGCAAAATATCTATCATAGGAAATGGCGTTGCTCTTGATTCACACGCTCTAATC
TCAGAAATAGAGTCATTGAAAGTTAAAGGAGTAGACGTAAACTATAACAACTTGATGGTATCTGAGAGCTGTCCATTAAT
ACTTAGCGTACATAAGGACAAGGAAAAGCTATTTGAAGATTTAAATGGAAATCACAAAATTGGTACAACAAATAAAGGGA
TAGGACCATGTTATGAAGATAAAGTTGGGAGGAGAGCTATACGCCTTTGTGACTTAGAAAACGCAGATGAGCTCAATAAA
AGATTGGATACTCTTTTGAATTACCACAATGCTATCAGGAAAGGCCTTAACTACCAGGTAGTTAAAAAAGAAGAAATATT
AAAGGAAATTCAAGAGATTTCAGAAAAAATTCTTTCATATAAAAAACCTGTGTGGAAGATATTAAATGACTTGATGAAAG
AAGGTAAGAAAATAATATTTGAAGGTGCCCAAGGCGCATTTTTAGATATCGACCACGGAACTTACCCTTTTGTTACTTCA
AGTAATACTGTAGCATCGCAAGCAATAACAGGCTCAGGGTTATCCTCCAATGCTTACATTATTGGTGTGGTAAAAGCTTA
TACAACAAGAGTGGGCAATGGCCCATTTCCTACTGAGCAGAAAAATGAAGTAGGAGATAGCTTGTTTACTATAGGTAAGG
AACTTGGAACAGTGAGCAATAGAAGAAGGCGTTGCGGATGGTTCGATGCAGTTTTAGTGCGCCAGGCCGTACAACTCTCT
GGAGTTTCAAGCATTGTATTAACCAAATTAGATGTGCTTAATTCTTTTGATACAATTAAAATATGCACTGGTTACAAGTA
TAGCGGAAAAATGTATGATTATTTACCCGCATCGCATTCAATACAAGGGGAATTAGAGCCAATATATGAAGAGTTTCCTG
GCTGGAAAGAAAACACTCAAGGTAAAAGGTCGATTGAAACATTGCCTATCAATTTAATAAAATATATAGAAGGGTTAGAA
AAATTAATAGGTGTACCAATTCATCTAATCTCAACTAGTCCAAAGAGAGAGGATGTTATTAAGCTTAAAGATTTCTGA

Upstream 100 bases:

>100_bases
TTGGAAACTCTTTATTTAAGAGGTGTAGGAAAGGCGTAGATTTCTCTTTCCATTTGTAGCGTACTGCATTAATATTTATG
TATTAATCTAGAAAATAATG

Downstream 100 bases:

>100_bases
AAACGTCTTTGTATCTTAGAGCCTGTACGTAATCTCAAAAAAGAGATAAAGTGAGAGGATAAAGTATATAAAGTAGGATA
TGCGAAGTTTATAACCAAGT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MNNIVIVGLQWGDEGKGKIVDYLSENADVVVRFQGGNNAGHTIVVDDEVYKLNLLPSAVLRPGKISIIGNGVALDSHALI
SEIESLKVKGVDVNYNNLMVSESCPLILSVHKDKEKLFEDLNGNHKIGTTNKGIGPCYEDKVGRRAIRLCDLENADELNK
RLDTLLNYHNAIRKGLNYQVVKKEEILKEIQEISEKILSYKKPVWKILNDLMKEGKKIIFEGAQGAFLDIDHGTYPFVTS
SNTVASQAITGSGLSSNAYIIGVVKAYTTRVGNGPFPTEQKNEVGDSLFTIGKELGTVSNRRRRCGWFDAVLVRQAVQLS
GVSSIVLTKLDVLNSFDTIKICTGYKYSGKMYDYLPASHSIQGELEPIYEEFPGWKENTQGKRSIETLPINLIKYIEGLE
KLIGVPIHLISTSPKREDVIKLKDF

Sequences:

>Translated_425_residues
MNNIVIVGLQWGDEGKGKIVDYLSENADVVVRFQGGNNAGHTIVVDDEVYKLNLLPSAVLRPGKISIIGNGVALDSHALI
SEIESLKVKGVDVNYNNLMVSESCPLILSVHKDKEKLFEDLNGNHKIGTTNKGIGPCYEDKVGRRAIRLCDLENADELNK
RLDTLLNYHNAIRKGLNYQVVKKEEILKEIQEISEKILSYKKPVWKILNDLMKEGKKIIFEGAQGAFLDIDHGTYPFVTS
SNTVASQAITGSGLSSNAYIIGVVKAYTTRVGNGPFPTEQKNEVGDSLFTIGKELGTVSNRRRRCGWFDAVLVRQAVQLS
GVSSIVLTKLDVLNSFDTIKICTGYKYSGKMYDYLPASHSIQGELEPIYEEFPGWKENTQGKRSIETLPINLIKYIEGLE
KLIGVPIHLISTSPKREDVIKLKDF
>Mature_425_residues
MNNIVIVGLQWGDEGKGKIVDYLSENADVVVRFQGGNNAGHTIVVDDEVYKLNLLPSAVLRPGKISIIGNGVALDSHALI
SEIESLKVKGVDVNYNNLMVSESCPLILSVHKDKEKLFEDLNGNHKIGTTNKGIGPCYEDKVGRRAIRLCDLENADELNK
RLDTLLNYHNAIRKGLNYQVVKKEEILKEIQEISEKILSYKKPVWKILNDLMKEGKKIIFEGAQGAFLDIDHGTYPFVTS
SNTVASQAITGSGLSSNAYIIGVVKAYTTRVGNGPFPTEQKNEVGDSLFTIGKELGTVSNRRRRCGWFDAVLVRQAVQLS
GVSSIVLTKLDVLNSFDTIKICTGYKYSGKMYDYLPASHSIQGELEPIYEEFPGWKENTQGKRSIETLPINLIKYIEGLE
KLIGVPIHLISTSPKREDVIKLKDF

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=425, Percent_Identity=41.1764705882353, Blast_Score=319, Evalue=4e-87,
Organism=Homo sapiens, GI34577063, Length=427, Percent_Identity=39.8126463700234, Blast_Score=316, Evalue=2e-86,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=39.6464646464646, Blast_Score=277, Evalue=1e-74,
Organism=Escherichia coli, GI1790620, Length=429, Percent_Identity=45.6876456876457, Blast_Score=379, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI25146555, Length=424, Percent_Identity=41.7452830188679, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI25146553, Length=424, Percent_Identity=41.7452830188679, Blast_Score=319, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6324109, Length=428, Percent_Identity=40.4205607476636, Blast_Score=306, Evalue=3e-84,
Organism=Drosophila melanogaster, GI21356033, Length=422, Percent_Identity=39.0995260663507, Blast_Score=308, Evalue=3e-84,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47173; Mature: 47173

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIVIVGLQWGDEGKGKIVDYLSENADVVVRFQGGNNAGHTIVVDDEVYKLNLLPSAVL
CCCEEEEEEEECCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEECCCEEEEECCHHHHC
RPGKISIIGNGVALDSHALISEIESLKVKGVDVNYNNLMVSESCPLILSVHKDKEKLFED
CCCEEEEEECCEEECHHHHHHHHHHCEEEEEEECCCCEEEECCCCEEEEECCCHHHHHHH
LNGNHKIGTTNKGIGPCYEDKVGRRAIRLCDLENADELNKRLDTLLNYHNAIRKGLNYQV
CCCCCEECCCCCCCCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
VKKEEILKEIQEISEKILSYKKPVWKILNDLMKEGKKIIFEGAQGAFLDIDHGTYPFVTS
ECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEC
SNTVASQAITGSGLSSNAYIIGVVKAYTTRVGNGPFPTEQKNEVGDSLFTIGKELGTVSN
CCCHHHHHEECCCCCCCCEEEEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RRRRCGWFDAVLVRQAVQLSGVSSIVLTKLDVLNSFDTIKICTGYKYSGKMYDYLPASHS
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECEEECCCEEECCCCCCC
IQGELEPIYEEFPGWKENTQGKRSIETLPINLIKYIEGLEKLIGVPIHLISTSPKREDVI
CCCCCCHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCE
KLKDF
EECCC
>Mature Secondary Structure
MNNIVIVGLQWGDEGKGKIVDYLSENADVVVRFQGGNNAGHTIVVDDEVYKLNLLPSAVL
CCCEEEEEEEECCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEECCCEEEEECCHHHHC
RPGKISIIGNGVALDSHALISEIESLKVKGVDVNYNNLMVSESCPLILSVHKDKEKLFED
CCCEEEEEECCEEECHHHHHHHHHHCEEEEEEECCCCEEEECCCCEEEEECCCHHHHHHH
LNGNHKIGTTNKGIGPCYEDKVGRRAIRLCDLENADELNKRLDTLLNYHNAIRKGLNYQV
CCCCCEECCCCCCCCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
VKKEEILKEIQEISEKILSYKKPVWKILNDLMKEGKKIIFEGAQGAFLDIDHGTYPFVTS
ECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEC
SNTVASQAITGSGLSSNAYIIGVVKAYTTRVGNGPFPTEQKNEVGDSLFTIGKELGTVSN
CCCHHHHHEECCCCCCCCEEEEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RRRRCGWFDAVLVRQAVQLSGVSSIVLTKLDVLNSFDTIKICTGYKYSGKMYDYLPASHS
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECEEECCCEEECCCCCCC
IQGELEPIYEEFPGWKENTQGKRSIETLPINLIKYIEGLEKLIGVPIHLISTSPKREDVI
CCCCCCHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCE
KLKDF
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA