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The map label for this gene is bcr [H]

Identifier: 225630205

GI number: 225630205

Start: 437627

End: 438790

Strand: Reverse

Name: bcr [H]

Synonym: WRi_004080

Alternate gene names: 225630205

Gene position: 438790-437627 (Counterclockwise)

Preceding gene: 225630206

Following gene: 225630204

Centisome position: 30.35

GC content: 35.05

Gene sequence:

>1164_bases
ATGATATTGTCTGTAGCAATTGTCGATATGGCAACTGACCTATACTCAGTTGCACTACCAAGCATTGCCAATTATTTTAA
AGTAGAAGGCAGTGTAGTGCAGCTTACAATTAGCTTAAATCTAGTTGGATTTGCGGTATCTGGCTTAATTTATGGTCCAT
TATCGGACCATTATGGTAGGCGTCCAATGATGCTGATTGGTATGACAATTTTTACTTTGGCAAGTGTCATGTGTTATATA
GCCGACAGTATTGCGCTCTTGATACTAATTCGCTTCATACAAGGAACAGGGGCTGGTGTTGCAGGTGTTGTTGGGTATGC
AGCAATAAGGGATATGTACTCGGGTAGTGAATACTCGAGAGTCATTTCAAAATTAAATATGGTTGTGGCACTCTCACCTG
GAATAGCTCCAGTGGTGGGCAGTTATATAATTTCACATGGGTATAGTTGGAAATTTTTGTTTTTTATTATATCACTTGCA
GCAATTGCTATACTTATTTTTATCTACTTTAAGTTACAAGAAACACTCACTGTAAATAAAAATGAAACCAGTATAACTAA
CATAACTACTAATATCTTCAAGCAATATATATCAATATTTAGAAATTATCGTTTCCTTGGGTTTTCAGCCATTCATGGAT
TAACTTTCATGTGGCTTTGGGCATACATTGCTAACTACCCGTTCATATTTGAATCGATGGGCATTGAAGTACAGTATTTT
GGCTACCTCATATCGATCATAGTCATATTTTACATAATTGGAACTTTAATTAATAGAAGGTATGTACCAAAAGTAGGGGT
TAGCAAAATGCTAATAATAGGTTTGGTGTTACCGATAATATCCGATAGTTTATTGGTATATTTTTATTTCGCGGACAAGT
TGAATATATTCATTCTTCAAACTGCTTGGATTCCAGCCAATATCGGACTTGCATTAATAATAAGCAATAACGTAACTTCT
GCTTTAGAAACGATCAAAGGTATAGGGCTTGGTAGTGCAGTTCTCTCATTCTGCAATATGATGTTTGGGGCTATTGGAAT
ATATATAGTAGGAAAATTTTTTCGCTATGGTATTTTACCAAATTTACTATTAACAATAGCATGCTCTACAATTGCAATTC
TTATGTACAGCCAGCTCAAATGTACTGAAAAACACAGGGAATAA

Upstream 100 bases:

>100_bases
TACTTATACTAATGAAAAAAATACATTATAATTAAGTATATTTAATCCAGTAAATCATTAAGTGAAAACAGCTCTTCCGC
AAAATATCATCTCTCTTATT

Downstream 100 bases:

>100_bases
CCATTATTTGCGTGATCCAGAGCTCATGGGTTATTAATGGCTTTATCATGATAATATAATTTTACAGGGGGTGAGAGCTC
ATAGATTATTAACGACTTTA

Product: bicyclomycin resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MILSVAIVDMATDLYSVALPSIANYFKVEGSVVQLTISLNLVGFAVSGLIYGPLSDHYGRRPMMLIGMTIFTLASVMCYI
ADSIALLILIRFIQGTGAGVAGVVGYAAIRDMYSGSEYSRVISKLNMVVALSPGIAPVVGSYIISHGYSWKFLFFIISLA
AIAILIFIYFKLQETLTVNKNETSITNITTNIFKQYISIFRNYRFLGFSAIHGLTFMWLWAYIANYPFIFESMGIEVQYF
GYLISIIVIFYIIGTLINRRYVPKVGVSKMLIIGLVLPIISDSLLVYFYFADKLNIFILQTAWIPANIGLALIISNNVTS
ALETIKGIGLGSAVLSFCNMMFGAIGIYIVGKFFRYGILPNLLLTIACSTIAILMYSQLKCTEKHRE

Sequences:

>Translated_387_residues
MILSVAIVDMATDLYSVALPSIANYFKVEGSVVQLTISLNLVGFAVSGLIYGPLSDHYGRRPMMLIGMTIFTLASVMCYI
ADSIALLILIRFIQGTGAGVAGVVGYAAIRDMYSGSEYSRVISKLNMVVALSPGIAPVVGSYIISHGYSWKFLFFIISLA
AIAILIFIYFKLQETLTVNKNETSITNITTNIFKQYISIFRNYRFLGFSAIHGLTFMWLWAYIANYPFIFESMGIEVQYF
GYLISIIVIFYIIGTLINRRYVPKVGVSKMLIIGLVLPIISDSLLVYFYFADKLNIFILQTAWIPANIGLALIISNNVTS
ALETIKGIGLGSAVLSFCNMMFGAIGIYIVGKFFRYGILPNLLLTIACSTIAILMYSQLKCTEKHRE
>Mature_387_residues
MILSVAIVDMATDLYSVALPSIANYFKVEGSVVQLTISLNLVGFAVSGLIYGPLSDHYGRRPMMLIGMTIFTLASVMCYI
ADSIALLILIRFIQGTGAGVAGVVGYAAIRDMYSGSEYSRVISKLNMVVALSPGIAPVVGSYIISHGYSWKFLFFIISLA
AIAILIFIYFKLQETLTVNKNETSITNITTNIFKQYISIFRNYRFLGFSAIHGLTFMWLWAYIANYPFIFESMGIEVQYF
GYLISIIVIFYIIGTLINRRYVPKVGVSKMLIIGLVLPIISDSLLVYFYFADKLNIFILQTAWIPANIGLALIISNNVTS
ALETIKGIGLGSAVLSFCNMMFGAIGIYIVGKFFRYGILPNLLLTIACSTIAILMYSQLKCTEKHRE

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=281, Percent_Identity=33.0960854092527, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI48994889, Length=268, Percent_Identity=29.4776119402985, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1790146, Length=163, Percent_Identity=34.9693251533742, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790794, Length=225, Percent_Identity=26.6666666666667, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787065, Length=228, Percent_Identity=28.5087719298246, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI87082312, Length=199, Percent_Identity=31.1557788944724, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081983, Length=190, Percent_Identity=28.9473684210526, Blast_Score=66, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323000, Length=314, Percent_Identity=26.1146496815287, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42888; Mature: 42888

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILSVAIVDMATDLYSVALPSIANYFKVEGSVVQLTISLNLVGFAVSGLIYGPLSDHYGR
CEEEEEHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEEHHHHHHHHHHEECCCHHCCCC
RPMMLIGMTIFTLASVMCYIADSIALLILIRFIQGTGAGVAGVVGYAAIRDMYSGSEYSR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHH
VISKLNMVVALSPGIAPVVGSYIISHGYSWKFLFFIISLAAIAILIFIYFKLQETLTVNK
HHHHHCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
NETSITNITTNIFKQYISIFRNYRFLGFSAIHGLTFMWLWAYIANYPFIFESMGIEVQYF
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHCCCCHHHH
GYLISIIVIFYIIGTLINRRYVPKVGVSKMLIIGLVLPIISDSLLVYFYFADKLNIFILQ
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEE
TAWIPANIGLALIISNNVTSALETIKGIGLGSAVLSFCNMMFGAIGIYIVGKFFRYGILP
EECCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLLLTIACSTIAILMYSQLKCTEKHRE
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MILSVAIVDMATDLYSVALPSIANYFKVEGSVVQLTISLNLVGFAVSGLIYGPLSDHYGR
CEEEEEHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEEHHHHHHHHHHEECCCHHCCCC
RPMMLIGMTIFTLASVMCYIADSIALLILIRFIQGTGAGVAGVVGYAAIRDMYSGSEYSR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHH
VISKLNMVVALSPGIAPVVGSYIISHGYSWKFLFFIISLAAIAILIFIYFKLQETLTVNK
HHHHHCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
NETSITNITTNIFKQYISIFRNYRFLGFSAIHGLTFMWLWAYIANYPFIFESMGIEVQYF
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHCCCCHHHH
GYLISIIVIFYIIGTLINRRYVPKVGVSKMLIIGLVLPIISDSLLVYFYFADKLNIFILQ
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEE
TAWIPANIGLALIISNNVTSALETIKGIGLGSAVLSFCNMMFGAIGIYIVGKFFRYGILP
EECCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLLLTIACSTIAILMYSQLKCTEKHRE
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]