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The map label for this gene is folD [H]

Identifier: 225630179

GI number: 225630179

Start: 403045

End: 403923

Strand: Reverse

Name: folD [H]

Synonym: WRi_003750

Alternate gene names: 225630179

Gene position: 403923-403045 (Counterclockwise)

Preceding gene: 225630180

Following gene: 225630176

Centisome position: 27.94

GC content: 37.32

Gene sequence:

>879_bases
GTGACAATTATTATTGATGGTAAAAAAATAGCAAACGACCTATGTGAGAGGCTATCACAAAAAATTGATATTTTAAAGAG
AGAGTATAACATTTTTCCTTGCCTGAAAGTAATTCTCGTGGGCAGCAATCCAGCAAGTCAGGTTTATGTTCGCAACAAAC
AGAAAAAAGCAGAATCAATAGGCATAAGTTCTGAGACCATTGTTTTGCCTGATAATATTTCAGAAGATGAATTAATTGAA
AAAATCAATGAATTAAATGAAGATCCATCTGTGCATGGCATTTTAGTGCAATTGCCTTTACCAAATCATATTAGTGCAAG
CAGAGTAATTAACACAGTAAGTGTTGAAAAAGATGTAGATGGCTTTCATGATGAAAACGTTGGCAAATTAGTTAAAGGTG
AAAAAAACTGCCTTATACCTTGCACTCCTAAAGGTTCTTTGCATTTAATTAAGTCAATTGAAGAGAACCTTTCCGGTAAA
AACGCAGTGGTAATTGGTAGGTCAAATATCGTGGGCAAGCCAATGTTTTACCTGCTATTGCAAGAAAACTGCACTGTCAC
CATCTTGCATTCACAAAGCAAGGATTTAGCTGAATATTGCTCTAAAGCGGATATAGTAGTTGCAGCAGTCGGAAAGCCAA
ATTTTGTTCAAGCAGACTGGATAAAGAAAGGTGCAATAGTGATCGATGTTGGCATAAATAGCGTTAACGTAGGAAAGCTT
GTAGGTGATGTTGACTTTGAAGGAATAAAAGGGAAAGCCAAAGCTATGACCCCAGTACCCGGGGGCGTTGGCCCAATGAC
CATTGCATTTCTAATGATGAACACTGTTATTGCTGCTTGCTTGCAGAAGGGAGTTGATGCTTCTAATTTCATTAGTTGA

Upstream 100 bases:

>100_bases
ATTAAACCGAAATCGTTGGATGATATTATTAATCATTCTGTTGGCAAAGTATTGGATTTATTTGATATCAAATTACCCGA
CTTTAAGGAGTGGCAAGGAA

Downstream 100 bases:

>100_bases
GGTATGAAGAGGAAGCTGTATGAAGATAGAATGTACCACATCTCACAATTAATAAAAAATTGAGATTTTTGAAATTTGTA
AAAGACAAGATTTGATCTGA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTIIIDGKKIANDLCERLSQKIDILKREYNIFPCLKVILVGSNPASQVYVRNKQKKAESIGISSETIVLPDNISEDELIE
KINELNEDPSVHGILVQLPLPNHISASRVINTVSVEKDVDGFHDENVGKLVKGEKNCLIPCTPKGSLHLIKSIEENLSGK
NAVVIGRSNIVGKPMFYLLLQENCTVTILHSQSKDLAEYCSKADIVVAAVGKPNFVQADWIKKGAIVIDVGINSVNVGKL
VGDVDFEGIKGKAKAMTPVPGGVGPMTIAFLMMNTVIAACLQKGVDASNFIS

Sequences:

>Translated_292_residues
MTIIIDGKKIANDLCERLSQKIDILKREYNIFPCLKVILVGSNPASQVYVRNKQKKAESIGISSETIVLPDNISEDELIE
KINELNEDPSVHGILVQLPLPNHISASRVINTVSVEKDVDGFHDENVGKLVKGEKNCLIPCTPKGSLHLIKSIEENLSGK
NAVVIGRSNIVGKPMFYLLLQENCTVTILHSQSKDLAEYCSKADIVVAAVGKPNFVQADWIKKGAIVIDVGINSVNVGKL
VGDVDFEGIKGKAKAMTPVPGGVGPMTIAFLMMNTVIAACLQKGVDASNFIS
>Mature_291_residues
TIIIDGKKIANDLCERLSQKIDILKREYNIFPCLKVILVGSNPASQVYVRNKQKKAESIGISSETIVLPDNISEDELIEK
INELNEDPSVHGILVQLPLPNHISASRVINTVSVEKDVDGFHDENVGKLVKGEKNCLIPCTPKGSLHLIKSIEENLSGKN
AVVIGRSNIVGKPMFYLLLQENCTVTILHSQSKDLAEYCSKADIVVAAVGKPNFVQADWIKKGAIVIDVGINSVNVGKLV
GDVDFEGIKGKAKAMTPVPGGVGPMTIAFLMMNTVIAACLQKGVDASNFIS

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=286, Percent_Identity=48.6013986013986, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI94721354, Length=293, Percent_Identity=48.4641638225256, Blast_Score=235, Evalue=3e-62,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=44.5205479452055, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI36796743, Length=198, Percent_Identity=30.8080808080808, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=49.4623655913978, Blast_Score=258, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17568735, Length=282, Percent_Identity=43.2624113475177, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=48.0836236933798, Blast_Score=266, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6321643, Length=296, Percent_Identity=44.9324324324324, Blast_Score=232, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6322933, Length=288, Percent_Identity=28.125, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI62472483, Length=286, Percent_Identity=45.4545454545455, Blast_Score=237, Evalue=8e-63,
Organism=Drosophila melanogaster, GI45551871, Length=286, Percent_Identity=45.4545454545455, Blast_Score=237, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24645718, Length=286, Percent_Identity=45.4545454545455, Blast_Score=236, Evalue=9e-63,
Organism=Drosophila melanogaster, GI17137370, Length=286, Percent_Identity=45.4545454545455, Blast_Score=236, Evalue=9e-63,
Organism=Drosophila melanogaster, GI17136818, Length=298, Percent_Identity=45.9731543624161, Blast_Score=235, Evalue=3e-62,
Organism=Drosophila melanogaster, GI17136816, Length=298, Percent_Identity=45.9731543624161, Blast_Score=235, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31542; Mature: 31411

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIIDGKKIANDLCERLSQKIDILKREYNIFPCLKVILVGSNPASQVYVRNKQKKAESI
CEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCHHHHHHC
GISSETIVLPDNISEDELIEKINELNEDPSVHGILVQLPLPNHISASRVINTVSVEKDVD
CCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHEEEECCCCCC
GFHDENVGKLVKGEKNCLIPCTPKGSLHLIKSIEENLSGKNAVVIGRSNIVGKPMFYLLL
CCCCCCCHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEE
QENCTVTILHSQSKDLAEYCSKADIVVAAVGKPNFVQADWIKKGAIVIDVGINSVNVGKL
ECCCEEEEEECCCHHHHHHCCCCCEEEEECCCCCEEEHHHCCCCCEEEEECCCCEEHHHE
VGDVDFEGIKGKAKAMTPVPGGVGPMTIAFLMMNTVIAACLQKGVDASNFIS
EECCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
TIIIDGKKIANDLCERLSQKIDILKREYNIFPCLKVILVGSNPASQVYVRNKQKKAESI
EEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCHHHHHHC
GISSETIVLPDNISEDELIEKINELNEDPSVHGILVQLPLPNHISASRVINTVSVEKDVD
CCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHEEEECCCCCC
GFHDENVGKLVKGEKNCLIPCTPKGSLHLIKSIEENLSGKNAVVIGRSNIVGKPMFYLLL
CCCCCCCHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEE
QENCTVTILHSQSKDLAEYCSKADIVVAAVGKPNFVQADWIKKGAIVIDVGINSVNVGKL
ECCCEEEEEECCCHHHHHHCCCCCEEEEECCCCCEEEHHHCCCCCEEEEECCCCEEHHHE
VGDVDFEGIKGKAKAMTPVPGGVGPMTIAFLMMNTVIAACLQKGVDASNFIS
EECCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA