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The map label for this gene is sucB [H]

Identifier: 225630166

GI number: 225630166

Start: 381869

End: 383041

Strand: Direct

Name: sucB [H]

Synonym: WRi_003570

Alternate gene names: 225630166

Gene position: 381869-383041 (Clockwise)

Preceding gene: 225630163

Following gene: 225630167

Centisome position: 26.41

GC content: 37.51

Gene sequence:

>1173_bases
ATGAGCAAAATTATAGAAATCAGAGCACCAAAAACTCTTGGTGGTGAATCAGTTACAGAAGGTATAGTAAAAATAAAGAA
GGGTATTGGAGAAGCAGTAAAAGTAGATGATCTGATTTTTGAAATTGAAACTGACAAAACAGCACTCGAATTAACTGCAG
AAGCTTCAGGGCAAATAACTGAATTTCTTGTAAAGGAAGATGATGTGATAAGTCCTGATCAATTGTTAGCGAAGCTTTCC
GTGGGAGAAGTAAAAGAGGAAGCGAAAAAAGAAGATAAAAGCGAAAGTGCTGCTAAAAAAGATGCTCCATCAGCTCGTAA
AATTATGGAAGAAAATGCAATCAGTGCAGAAAGCGTCAAAGGAACTGGCATGGGAGGCAGAATAACAAAAGCAGATGTGA
TAGGCCATATGAACAAAGCTGAACAACCTGCAATAAAACAATATGAATTGCCAAAAAGTGTAGTAAATGGAGAGCAAAGA
GAGGAACGAGTAAAAATGAGCAAAATAAGGCAAGTAATTGCCGCTCGTTTGAAAGCATCGCAAAATACTGCTGCAATACT
GACCACGTTCAATGAAATTGACATGAAGAATGTCATGGATCTAAGAACGAAATATAAGGACGCCTTTGAAAAAAAATATG
GAATAAAACTTGGTTTTATGTCGTTTTTTATAAAGGCAGCGGTGCAAGCACTGAAAGAGATTCCTGAAATTAACGCTGAA
ATCTCAGGCGATGAAATCATATATAAACATTACTATGACATAGGTGTTGCTGTTGGCACTGACAAAGGCCTTGTTGTGCC
GGTTATTCGGAGTGCCGATCAGATGTCATTTGCCGAAATTGAATTGACTTTAGTTGCTCTTGGCAAAAAAGCACGAGAAG
GTAAATTACAAGTATCAGAAATGGAAGGTGCAACATTTACCATTTCAAATGGCGGAGTATATGGTTCGCTCCTTTCTACT
CCGATAATAAATCCTCCGCAATCTGGAATACTTGGAATGCATTCAATACAAAACAGGCCAGTTGCTGTTGGTAATGCAGT
AGAGATCAGACCTATGATGTACATTGCTCTTTCTTATGATCACAGAATAGTTGATGGCAAAGGAGCAGTGACTTTCCTCG
TTAAAATAAAAAATTATATAGAAGATCCAAATAGGTTAGTTTTGGAAGTGTAG

Upstream 100 bases:

>100_bases
TGTCACTTCTAAATGATAAGAGCCTCAATGAATTTATTAGTTTATTACATGTCTCTTTTCATGTATAAATCTATAGAAAG
TTTTATACGGAAGTTGTTTT

Downstream 100 bases:

>100_bases
CTCAAATGAGTTGCTGTTAGCCTAGTATTGCTAAAAACCTAAACGTCATCCAATTCTGCCAATAGTTTCACAACTTCCGG
GTGATCAGGGCCGTAATGTT

Product: 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSKIIEIRAPKTLGGESVTEGIVKIKKGIGEAVKVDDLIFEIETDKTALELTAEASGQITEFLVKEDDVISPDQLLAKLS
VGEVKEEAKKEDKSESAAKKDAPSARKIMEENAISAESVKGTGMGGRITKADVIGHMNKAEQPAIKQYELPKSVVNGEQR
EERVKMSKIRQVIAARLKASQNTAAILTTFNEIDMKNVMDLRTKYKDAFEKKYGIKLGFMSFFIKAAVQALKEIPEINAE
ISGDEIIYKHYYDIGVAVGTDKGLVVPVIRSADQMSFAEIELTLVALGKKAREGKLQVSEMEGATFTISNGGVYGSLLST
PIINPPQSGILGMHSIQNRPVAVGNAVEIRPMMYIALSYDHRIVDGKGAVTFLVKIKNYIEDPNRLVLEV

Sequences:

>Translated_390_residues
MSKIIEIRAPKTLGGESVTEGIVKIKKGIGEAVKVDDLIFEIETDKTALELTAEASGQITEFLVKEDDVISPDQLLAKLS
VGEVKEEAKKEDKSESAAKKDAPSARKIMEENAISAESVKGTGMGGRITKADVIGHMNKAEQPAIKQYELPKSVVNGEQR
EERVKMSKIRQVIAARLKASQNTAAILTTFNEIDMKNVMDLRTKYKDAFEKKYGIKLGFMSFFIKAAVQALKEIPEINAE
ISGDEIIYKHYYDIGVAVGTDKGLVVPVIRSADQMSFAEIELTLVALGKKAREGKLQVSEMEGATFTISNGGVYGSLLST
PIINPPQSGILGMHSIQNRPVAVGNAVEIRPMMYIALSYDHRIVDGKGAVTFLVKIKNYIEDPNRLVLEV
>Mature_389_residues
SKIIEIRAPKTLGGESVTEGIVKIKKGIGEAVKVDDLIFEIETDKTALELTAEASGQITEFLVKEDDVISPDQLLAKLSV
GEVKEEAKKEDKSESAAKKDAPSARKIMEENAISAESVKGTGMGGRITKADVIGHMNKAEQPAIKQYELPKSVVNGEQRE
ERVKMSKIRQVIAARLKASQNTAAILTTFNEIDMKNVMDLRTKYKDAFEKKYGIKLGFMSFFIKAAVQALKEIPEINAEI
SGDEIIYKHYYDIGVAVGTDKGLVVPVIRSADQMSFAEIELTLVALGKKAREGKLQVSEMEGATFTISNGGVYGSLLSTP
IINPPQSGILGMHSIQNRPVAVGNAVEIRPMMYIALSYDHRIVDGKGAVTFLVKIKNYIEDPNRLVLEV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=236, Percent_Identity=58.8983050847458, Blast_Score=299, Evalue=3e-81,
Organism=Homo sapiens, GI31711992, Length=300, Percent_Identity=33, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI203098753, Length=435, Percent_Identity=29.1954022988506, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI203098816, Length=435, Percent_Identity=29.1954022988506, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI110671329, Length=420, Percent_Identity=28.0952380952381, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=39.1304347826087, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1786946, Length=393, Percent_Identity=45.5470737913486, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1786305, Length=427, Percent_Identity=29.2740046838407, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI25146366, Length=406, Percent_Identity=44.3349753694581, Blast_Score=325, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17560088, Length=425, Percent_Identity=28.9411764705882, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17537937, Length=426, Percent_Identity=28.6384976525822, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=234, Percent_Identity=32.9059829059829, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=381, Percent_Identity=43.8320209973753, Blast_Score=314, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6324258, Length=331, Percent_Identity=28.7009063444109, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24645909, Length=231, Percent_Identity=59.7402597402597, Blast_Score=291, Evalue=5e-79,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=34.9137931034483, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=34.9137931034483, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI18859875, Length=421, Percent_Identity=26.1282660332542, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 42645; Mature: 42514

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIIEIRAPKTLGGESVTEGIVKIKKGIGEAVKVDDLIFEIETDKTALELTAEASGQIT
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCEEEEEEECCCCCHH
EFLVKEDDVISPDQLLAKLSVGEVKEEAKKEDKSESAAKKDAPSARKIMEENAISAESVK
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHCCC
GTGMGGRITKADVIGHMNKAEQPAIKQYELPKSVVNGEQREERVKMSKIRQVIAARLKAS
CCCCCCCEEHHHHHHCCCCCCCCCHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
QNTAAILTTFNEIDMKNVMDLRTKYKDAFEKKYGIKLGFMSFFIKAAVQALKEIPEINAE
CCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
ISGDEIIYKHYYDIGVAVGTDKGLVVPVIRSADQMSFAEIELTLVALGKKAREGKLQVSE
CCCCEEEEEEEEEEEEEEECCCCEEEEEECCCCCCCHHHEEEEEEECCCCCCCCCEEEEE
MEGATFTISNGGVYGSLLSTPIINPPQSGILGMHSIQNRPVAVGNAVEIRPMMYIALSYD
CCCCEEEEECCCEEHHHHCCCCCCCCCCCCEEHHHCCCCCEEECCCEEECEEEEEEEECC
HRIVDGKGAVTFLVKIKNYIEDPNRLVLEV
CEEECCCCCEEEHEEHHHHHCCCCEEEEEC
>Mature Secondary Structure 
SKIIEIRAPKTLGGESVTEGIVKIKKGIGEAVKVDDLIFEIETDKTALELTAEASGQIT
CCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCEEEEEEECCCCCHH
EFLVKEDDVISPDQLLAKLSVGEVKEEAKKEDKSESAAKKDAPSARKIMEENAISAESVK
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHCCC
GTGMGGRITKADVIGHMNKAEQPAIKQYELPKSVVNGEQREERVKMSKIRQVIAARLKAS
CCCCCCCEEHHHHHHCCCCCCCCCHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
QNTAAILTTFNEIDMKNVMDLRTKYKDAFEKKYGIKLGFMSFFIKAAVQALKEIPEINAE
CCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
ISGDEIIYKHYYDIGVAVGTDKGLVVPVIRSADQMSFAEIELTLVALGKKAREGKLQVSE
CCCCEEEEEEEEEEEEEEECCCCEEEEEECCCCCCCHHHEEEEEEECCCCCCCCCEEEEE
MEGATFTISNGGVYGSLLSTPIINPPQSGILGMHSIQNRPVAVGNAVEIRPMMYIALSYD
CCCCEEEEECCCEEHHHHCCCCCCCCCCCCEEHHHCCCCCEEECCCEEECEEEEEEEECC
HRIVDGKGAVTFLVKIKNYIEDPNRLVLEV
CEEECCCCCEEEHEEHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]