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The map label for this gene is ltrA [H]

Identifier: 225630161

GI number: 225630161

Start: 375621

End: 377168

Strand: Direct

Name: ltrA [H]

Synonym: WRi_003510

Alternate gene names: 225630161

Gene position: 375621-377168 (Clockwise)

Preceding gene: 225630160

Following gene: 225630162

Centisome position: 25.98

GC content: 38.31

Gene sequence:

>1548_bases
ATGATTACAAGTACAACTGTAAGTGCACCTACCAATAACTCCGAAGCATGGAACCAGTTACCCTGGAAGAAATGCCAAAA
AGTTGTTATGAGGCTACAGAGGCGTATTGTTAAGGCTGTCCAACAAGGCAGATGGGGTAAGGTGAAAACTTTACAACACC
TTCTCACACGCTCTTTTAGCGGCAAAGCTTTGGCGGTTAAGAGAGTAACTGAAAACCAAGGAAAAAACACAGCAGGTGTA
GATCGTCAAATATGGTCAACTTGCAACACAAAATTTCAAGGAATAAAGCTATTAAAACAAAGAGGATACAAACCTTCTCC
GCTAAAACGGATATACATCAGTAAGTCTAATGGCAAAAGAAGACCTCTTGGAATCCCTACGATAAAAGATAGAGCCATGC
AAGCATTATACTTGTTTGCTTTGGAACCGATAGCTGAAACGATCAGTGATCGTCACTCTTATGGTTTTAGACCAAAAAGA
TCCTGCGCAGACGCTACTGTGGCTTGTCACTTGTTACTGGCAAGCCGTAACCAACTACAATGGATACTCGAAGGTGATAT
TAAAGGGTGTTTTGATAACATTAACCATGAATGGCTCATGAAGCATATTCCTATGGAGAAGAAAATTCTTCATAGCTGGT
TAAAAGCTGGCTTCCTAGAATCAAAAACTCTGTATTCCACAACTGCAGGTACCCCACAAGGTAGTATCATCTCTCCAATA
CTAGCAAATCTAGCCCTAAATGGACTTGAAAAATCATTGGAAAGTCAATTTGGTAAACTTGGCAGTAAAAGAAGAAGCAA
AATCAGAAGTGGAGTAAATGTAATCAGGTACGCAGATGACTTTATCATTTCAGGAATCACACGTGAAGTACTGGAAAATG
AAGTAAAACCTCTAGTATCGTCCTTTCTTCAGGAGAGAGGTCTTATCCTTTCCGAAGAGAAAACAAAAATTACATCTATT
ACAACAGGGTTTGACTTTCTTGGTTGTAATGTACGCAGGTATAATAAGAAGTTAATTATAAAACCTTCAAAAGAAAGTAT
TAAGAAACTTCTTAATAAAGCACGTACATTGATAAAGGCAAACATAGCGAACACTCAGGCCGTACTAATCAAGTTGCTCA
ATTCCCTATTAAGGGGATGGGGAAATTACTACAGCCATGTGTGTGCGAAAAGAGCTTTTAACAAAATAGACCATGAAATT
ATGTGTGCTCTATGGAAATGGGCAAAGAAAAGACATCCTCGTAAAGGATTACGTTGGATAAAGAATCGTTACTTCAAGGT
AATGAAACAACGCCAATGGGTCTTTGCTGCACCCATATGCAAGAACAAACCGAAAGAGATAAGGTATTTAAGACTGCTTA
GGTTGATTGATATACCTATCAGACGACATGTTAAAATCAGGGCGGATGCAAATCCACTTGACTTAAAATGGAAAAAGTAT
TTTGATGAAAGAGTGAAACAAACAAAAATGTTAGCAAGCTCTTTCTCAAGAGAAGGTTCTCTACTGTTGGTGTCACCATT
AAGAATGATGTTTTCTGAGGAATCATGA

Upstream 100 bases:

>100_bases
TGGATAGAATTTGTAACATTAATTCGCGTGAAAACGAGCAGACTTCCATTTGGTCCCTCATGACAAGAAACTTTGTTAAC
CACTTAAGGTAGGAAAGCAG

Downstream 100 bases:

>100_bases
AGGACAAGCCGGTTCGAGAAAAGAAGGACTTAGATAAGGGGCTCAAGCGTAGTGTGGGGAAACCTGCATGCTGCGTTTCT
AAGGGAGGGAGTAGTAGCAA

Product: reverse transcriptase, putative

Products: NA

Alternate protein names: Reverse-transcriptase; RNA maturase; DNA endonuclease [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MITSTTVSAPTNNSEAWNQLPWKKCQKVVMRLQRRIVKAVQQGRWGKVKTLQHLLTRSFSGKALAVKRVTENQGKNTAGV
DRQIWSTCNTKFQGIKLLKQRGYKPSPLKRIYISKSNGKRRPLGIPTIKDRAMQALYLFALEPIAETISDRHSYGFRPKR
SCADATVACHLLLASRNQLQWILEGDIKGCFDNINHEWLMKHIPMEKKILHSWLKAGFLESKTLYSTTAGTPQGSIISPI
LANLALNGLEKSLESQFGKLGSKRRSKIRSGVNVIRYADDFIISGITREVLENEVKPLVSSFLQERGLILSEEKTKITSI
TTGFDFLGCNVRRYNKKLIIKPSKESIKKLLNKARTLIKANIANTQAVLIKLLNSLLRGWGNYYSHVCAKRAFNKIDHEI
MCALWKWAKKRHPRKGLRWIKNRYFKVMKQRQWVFAAPICKNKPKEIRYLRLLRLIDIPIRRHVKIRADANPLDLKWKKY
FDERVKQTKMLASSFSREGSLLLVSPLRMMFSEES

Sequences:

>Translated_515_residues
MITSTTVSAPTNNSEAWNQLPWKKCQKVVMRLQRRIVKAVQQGRWGKVKTLQHLLTRSFSGKALAVKRVTENQGKNTAGV
DRQIWSTCNTKFQGIKLLKQRGYKPSPLKRIYISKSNGKRRPLGIPTIKDRAMQALYLFALEPIAETISDRHSYGFRPKR
SCADATVACHLLLASRNQLQWILEGDIKGCFDNINHEWLMKHIPMEKKILHSWLKAGFLESKTLYSTTAGTPQGSIISPI
LANLALNGLEKSLESQFGKLGSKRRSKIRSGVNVIRYADDFIISGITREVLENEVKPLVSSFLQERGLILSEEKTKITSI
TTGFDFLGCNVRRYNKKLIIKPSKESIKKLLNKARTLIKANIANTQAVLIKLLNSLLRGWGNYYSHVCAKRAFNKIDHEI
MCALWKWAKKRHPRKGLRWIKNRYFKVMKQRQWVFAAPICKNKPKEIRYLRLLRLIDIPIRRHVKIRADANPLDLKWKKY
FDERVKQTKMLASSFSREGSLLLVSPLRMMFSEES
>Mature_515_residues
MITSTTVSAPTNNSEAWNQLPWKKCQKVVMRLQRRIVKAVQQGRWGKVKTLQHLLTRSFSGKALAVKRVTENQGKNTAGV
DRQIWSTCNTKFQGIKLLKQRGYKPSPLKRIYISKSNGKRRPLGIPTIKDRAMQALYLFALEPIAETISDRHSYGFRPKR
SCADATVACHLLLASRNQLQWILEGDIKGCFDNINHEWLMKHIPMEKKILHSWLKAGFLESKTLYSTTAGTPQGSIISPI
LANLALNGLEKSLESQFGKLGSKRRSKIRSGVNVIRYADDFIISGITREVLENEVKPLVSSFLQERGLILSEEKTKITSI
TTGFDFLGCNVRRYNKKLIIKPSKESIKKLLNKARTLIKANIANTQAVLIKLLNSLLRGWGNYYSHVCAKRAFNKIDHEI
MCALWKWAKKRHPRKGLRWIKNRYFKVMKQRQWVFAAPICKNKPKEIRYLRLLRLIDIPIRRHVKIRADANPLDLKWKKY
FDERVKQTKMLASSFSREGSLLLVSPLRMMFSEES

Specific function: Multifunctional protein that promotes group II intron splicing and mobility by acting both on RNA and DNA. It has three activities:reverse transcriptase (RT) for intron duplication, maturase to promote splicing, and DNA endonuclease for site- specific cle

COG id: COG3344

COG function: function code L; Retron-type reverse transcriptase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 reverse transcriptase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6226521, Length=283, Percent_Identity=27.5618374558304, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6226520, Length=318, Percent_Identity=28.3018867924528, Blast_Score=104, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR002866
- InterPro:   IPR000477 [H]

Pfam domain/function: PF01348 Intron_maturas2; PF00078 RVT_1 [H]

EC number: =2.7.7.49 [H]

Molecular weight: Translated: 59299; Mature: 59299

Theoretical pI: Translated: 11.13; Mature: 11.13

Prosite motif: PS50878 RT_POL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSTTVSAPTNNSEAWNQLPWKKCQKVVMRLQRRIVKAVQQGRWGKVKTLQHLLTRSFS
CCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
GKALAVKRVTENQGKNTAGVDRQIWSTCNTKFQGIKLLKQRGYKPSPLKRIYISKSNGKR
CCEEEEEEHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
RPLGIPTIKDRAMQALYLFALEPIAETISDRHSYGFRPKRSCADATVACHLLLASRNQLQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEE
WILEGDIKGCFDNINHEWLMKHIPMEKKILHSWLKAGFLESKTLYSTTAGTPQGSIISPI
EEEECCHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHH
LANLALNGLEKSLESQFGKLGSKRRSKIRSGVNVIRYADDFIISGITREVLENEVKPLVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFLQERGLILSEEKTKITSITTGFDFLGCNVRRYNKKLIIKPSKESIKKLLNKARTLIKA
HHHHHCCCEEECCCCHHEEEECCCHHHCCCCEEECCEEEECCCHHHHHHHHHHHHHHHHH
NIANTQAVLIKLLNSLLRGWGNYYSHVCAKRAFNKIDHEIMCALWKWAKKRHPRKGLRWI
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KNRYFKVMKQRQWVFAAPICKNKPKEIRYLRLLRLIDIPIRRHVKIRADANPLDLKWKKY
HHHHHHHHHHCCCEEECCHHCCCCHHHHHHHHHHHHHCCHHCCEEEEECCCCCCCHHHHH
FDERVKQTKMLASSFSREGSLLLVSPLRMMFSEES
HHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHCCCC
>Mature Secondary Structure
MITSTTVSAPTNNSEAWNQLPWKKCQKVVMRLQRRIVKAVQQGRWGKVKTLQHLLTRSFS
CCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
GKALAVKRVTENQGKNTAGVDRQIWSTCNTKFQGIKLLKQRGYKPSPLKRIYISKSNGKR
CCEEEEEEHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
RPLGIPTIKDRAMQALYLFALEPIAETISDRHSYGFRPKRSCADATVACHLLLASRNQLQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEE
WILEGDIKGCFDNINHEWLMKHIPMEKKILHSWLKAGFLESKTLYSTTAGTPQGSIISPI
EEEECCHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHH
LANLALNGLEKSLESQFGKLGSKRRSKIRSGVNVIRYADDFIISGITREVLENEVKPLVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFLQERGLILSEEKTKITSITTGFDFLGCNVRRYNKKLIIKPSKESIKKLLNKARTLIKA
HHHHHCCCEEECCCCHHEEEECCCHHHCCCCEEECCEEEECCCHHHHHHHHHHHHHHHHH
NIANTQAVLIKLLNSLLRGWGNYYSHVCAKRAFNKIDHEIMCALWKWAKKRHPRKGLRWI
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KNRYFKVMKQRQWVFAAPICKNKPKEIRYLRLLRLIDIPIRRHVKIRADANPLDLKWKKY
HHHHHHHHHHCCCEEECCHHCCCCHHHHHHHHHHHHHCCHHCCEEEEECCCCCCCHHHHH
FDERVKQTKMLASSFSREGSLLLVSPLRMMFSEES
HHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8655550; 11157264; 10488339; 9353259; 10413481; 11731786 [H]