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The map label for this gene is pdhA [H]

Identifier: 225630143

GI number: 225630143

Start: 353940

End: 354920

Strand: Direct

Name: pdhA [H]

Synonym: WRi_003250

Alternate gene names: 225630143

Gene position: 353940-354920 (Clockwise)

Preceding gene: 225630140

Following gene: 225630144

Centisome position: 24.48

GC content: 37.82

Gene sequence:

>981_bases
ATGAAAGCAGAAAATTTCACTAAAGAACAGATAATTGGATTCTACAGGAAAATGCTACTCATACGCAGATTTGAGGAAAA
AGCAGGACAATTATATGGGATGGGATTAATAGGCGGGTTCTGTCACCTATCAATAGGACAAGAAGCAGTTGCAGTTGGAA
CTCAAGCTGCATCAAAATTAGGTGACGCTTTTATCACAAGTTATAGAGACCATGGCTTAATGCTCGCATGTGATTCTGAT
CCAAATGTTGTAATGGCAGAGCTTACCGGCAAAGAAACAGGATGCTCGAAAGGTAAAGGTGGTTCGATGCACGTATTTGA
CGTTGAAAAAAAATTCTTCGGCGGGCATGGAATAGTGGGAGCACAAGTTCCAATTGGCACAGGAATAGCATTTGCTAATA
AATATAAGAAAAAAGATAATGTGGTATTTACATATTTTGGCGACGGTGCTGCAAATCAAGGACAAGTATATGAATCGTTT
AATATGGCATCTTTGTGGGAGTTGCCTGTGGTTTACATCATAGAAAATAATGAATACGCAATGGGAACCTCCGTGCAAAG
GTCAACTTTAGTAACAGAGTTATATAAAAGAGGAGAAAGTTTTGGTATTCCTGGAAAACAAGTTGATGGAATGGATTTTT
TCTCTGTTTATGAGGCTACAAGCGAAGCAGCGGAACACACACGCAGCGGAAAAGGGCCTATCCTGCTTGAAATGAAGACA
TATCGATATCGCGGTCACTCAATGTCAGATCCTGCTACTTATCGCTTAAAAGAAGAAGTTGAAGATATGAAGCAAAATCA
TGACCCTATAAGCACTTTAAAGAAATACATGACAGATAATAAAATGGCTTCAGAAGAAGAGTGCAAAATAATTGATAAGG
AAATACGTGATTTAGTAAAAAAGTCGGAAGATTTTGCCAAAAATAGTAAAGAACCAAGCGTTGATGAGCTGTATACTGAT
GTTTATAAATTTGTTAGCTAA

Upstream 100 bases:

>100_bases
AACAGAGATAACGTGAAAAGCCTCAAGCCAGTGGTAATAGTTCATTAGCAAAAATTTAAAATTCCTGGTATTGTGTTAGT
TTAATGAAAAAGAAATCGAT

Downstream 100 bases:

>100_bases
TTTTTTTATCCAGAACTGTACAAACATTGTATAGTAAACGATGTCATCTAAATAGCTCCTTCTCCCGTCATCCAAGTGCC
CTCCTTTTTTTGTCATCCCA

Product: pyruvate dehydrogenase complex, E1 component, pyruvate dehydrogenase alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKAENFTKEQIIGFYRKMLLIRRFEEKAGQLYGMGLIGGFCHLSIGQEAVAVGTQAASKLGDAFITSYRDHGLMLACDSD
PNVVMAELTGKETGCSKGKGGSMHVFDVEKKFFGGHGIVGAQVPIGTGIAFANKYKKKDNVVFTYFGDGAANQGQVYESF
NMASLWELPVVYIIENNEYAMGTSVQRSTLVTELYKRGESFGIPGKQVDGMDFFSVYEATSEAAEHTRSGKGPILLEMKT
YRYRGHSMSDPATYRLKEEVEDMKQNHDPISTLKKYMTDNKMASEEECKIIDKEIRDLVKKSEDFAKNSKEPSVDELYTD
VYKFVS

Sequences:

>Translated_326_residues
MKAENFTKEQIIGFYRKMLLIRRFEEKAGQLYGMGLIGGFCHLSIGQEAVAVGTQAASKLGDAFITSYRDHGLMLACDSD
PNVVMAELTGKETGCSKGKGGSMHVFDVEKKFFGGHGIVGAQVPIGTGIAFANKYKKKDNVVFTYFGDGAANQGQVYESF
NMASLWELPVVYIIENNEYAMGTSVQRSTLVTELYKRGESFGIPGKQVDGMDFFSVYEATSEAAEHTRSGKGPILLEMKT
YRYRGHSMSDPATYRLKEEVEDMKQNHDPISTLKKYMTDNKMASEEECKIIDKEIRDLVKKSEDFAKNSKEPSVDELYTD
VYKFVS
>Mature_326_residues
MKAENFTKEQIIGFYRKMLLIRRFEEKAGQLYGMGLIGGFCHLSIGQEAVAVGTQAASKLGDAFITSYRDHGLMLACDSD
PNVVMAELTGKETGCSKGKGGSMHVFDVEKKFFGGHGIVGAQVPIGTGIAFANKYKKKDNVVFTYFGDGAANQGQVYESF
NMASLWELPVVYIIENNEYAMGTSVQRSTLVTELYKRGESFGIPGKQVDGMDFFSVYEATSEAAEHTRSGKGPILLEMKT
YRYRGHSMSDPATYRLKEEVEDMKQNHDPISTLKKYMTDNKMASEEECKIIDKEIRDLVKKSEDFAKNSKEPSVDELYTD
VYKFVS

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=318, Percent_Identity=50.6289308176101, Blast_Score=326, Evalue=2e-89,
Organism=Homo sapiens, GI291084742, Length=318, Percent_Identity=51.5723270440252, Blast_Score=312, Evalue=3e-85,
Organism=Homo sapiens, GI4505685, Length=318, Percent_Identity=51.5723270440252, Blast_Score=311, Evalue=4e-85,
Organism=Homo sapiens, GI291084744, Length=325, Percent_Identity=50.4615384615385, Blast_Score=304, Evalue=6e-83,
Organism=Homo sapiens, GI291084757, Length=318, Percent_Identity=45.2830188679245, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI11386135, Length=320, Percent_Identity=30.625, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI258645172, Length=325, Percent_Identity=31.0769230769231, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI32564172, Length=319, Percent_Identity=49.2163009404389, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17536047, Length=319, Percent_Identity=49.2163009404389, Blast_Score=317, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI86563357, Length=320, Percent_Identity=30, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI86563355, Length=320, Percent_Identity=30, Blast_Score=138, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6321026, Length=319, Percent_Identity=48.9028213166144, Blast_Score=311, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24639744, Length=318, Percent_Identity=47.1698113207547, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI28571106, Length=318, Percent_Identity=47.1698113207547, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24639740, Length=318, Percent_Identity=47.1698113207547, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=47.7124183006536, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24639748, Length=318, Percent_Identity=47.7987421383648, Blast_Score=293, Evalue=9e-80,
Organism=Drosophila melanogaster, GI21355903, Length=318, Percent_Identity=28.6163522012579, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR017597 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36369; Mature: 36369

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAENFTKEQIIGFYRKMLLIRRFEEKAGQLYGMGLIGGFCHLSIGQEAVAVGTQAASKL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHEECCCCHHHHHHHHHHHHH
GDAFITSYRDHGLMLACDSDPNVVMAELTGKETGCSKGKGGSMHVFDVEKKFFGGHGIVG
HHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEECHHHHCCCCCEEE
AQVPIGTGIAFANKYKKKDNVVFTYFGDGAANQGQVYESFNMASLWELPVVYIIENNEYA
CCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCEEECCCEEEEEECCCEE
MGTSVQRSTLVTELYKRGESFGIPGKQVDGMDFFSVYEATSEAAEHTRSGKGPILLEMKT
ECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
YRYRGHSMSDPATYRLKEEVEDMKQNHDPISTLKKYMTDNKMASEEECKIIDKEIRDLVK
EEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
KSEDFAKNSKEPSVDELYTDVYKFVS
HHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKAENFTKEQIIGFYRKMLLIRRFEEKAGQLYGMGLIGGFCHLSIGQEAVAVGTQAASKL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHEECCCCHHHHHHHHHHHHH
GDAFITSYRDHGLMLACDSDPNVVMAELTGKETGCSKGKGGSMHVFDVEKKFFGGHGIVG
HHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEECHHHHCCCCCEEE
AQVPIGTGIAFANKYKKKDNVVFTYFGDGAANQGQVYESFNMASLWELPVVYIIENNEYA
CCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCEEECCCEEEEEECCCEE
MGTSVQRSTLVTELYKRGESFGIPGKQVDGMDFFSVYEATSEAAEHTRSGKGPILLEMKT
ECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
YRYRGHSMSDPATYRLKEEVEDMKQNHDPISTLKKYMTDNKMASEEECKIIDKEIRDLVK
EEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
KSEDFAKNSKEPSVDELYTDVYKFVS
HHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]