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The map label for this gene is cca [H]

Identifier: 225630119

GI number: 225630119

Start: 319263

End: 320435

Strand: Reverse

Name: cca [H]

Synonym: WRi_003010

Alternate gene names: 225630119

Gene position: 320435-319263 (Counterclockwise)

Preceding gene: 225630120

Following gene: 225630116

Centisome position: 22.16

GC content: 35.55

Gene sequence:

>1173_bases
ATGCAAGTTGACCAGGAGACTAGTTTAATTATTGATGCCATAGAGGAATTTGGTGGTGAGGCTAGGCTTGTCGGCGGATG
TGTGAGAGACTCAATTTTGCAACGTGATGTCCACGACATCGATTTAGCTACTAATTTGCTTCCCAATCAAACAATTAAAG
CGCTAAAACTCCGTAATATAAAAACTATTCCAACTGGCTTAAACCATGGAACTATTACTGCAATCTTAAATCAGAGGTCT
TTTGAAATTACGACACTAAGGCATGATGTGAAATGTGATGGTAGGCATGCAAAAGTAGAATTTACTAATAATTGGCAAGC
TGATGCTTCAAGGCGTGACTTTACATTTAACGCTCTATATGCAGATAAGCATGGCCATATATATGACTACTTTGGTGGCA
TTGAGGATTTAAAGGTGCGAAGGTTAAACTTTATAGGCAACGCTGAAGATAGAATTAAAGAAGACTATCTACGTATTTTA
AGAGCATTTCGTTTTCATGCAAAAATATGTGTCGGAGATTTGAGTGATGAAATACTAAACGTATGCAAAAAGCATTCGCA
TATGATCCAAAACCTCTCTGGAGAGAGAATAAGAGATGAAATACTTAAATTGCTGGAGTGCAATGATCCTTTTCCAACAC
TTAAGAGCATGCAAGAATCTGATGTTTTGCAAAAGATTATCCCAAAAGAAGTAAAATGCGAAATTCTGTCTTCGTCACTT
CTTTTCGGCACTGATGCACTAGTAAAATTAGCGTTACTCCTTAGGACCACCAAAAATGACAGGCTAAGTCTTGGAGAATA
TGTAAGTAAGTTTCTACGTCTTTCAAACAAGCAAAAGAAAAAGCTGTTATTTTTATTATCCAACGATATCAAAACAGAAC
TCTCAGAAAAAGAGCAAAAAAAATACATATCTTTATTTGGTAGAGAATTATATTGTGATTTAGTGAAAATTTGTGGTGTT
GAGTCTGGAGAAAATGTTGATGAATACATTTCATTTGCTAACACATTCAATATTCCAAAATTTCCTTTATCTGGCGATGA
TTTAATAAGTATAGGTCACCAGCCAGGAAAAAGTTTAGGTAGAAACTTGGAATTGCTAAAACAACATTGGGAAGATAGCT
CCTACACTTTAACAAAAGAGGAGCTGGTGCTTTATGCTAAGAGCCTATTCTAA

Upstream 100 bases:

>100_bases
AAATACCTGCACAGTAAATTTTCATAGCCCTGTATATTTTTATATAGGGGTTTGTGCAAGCATTTCATATAATATGAACT
TGCAGTATGTACTAACCTAA

Downstream 100 bases:

>100_bases
CTGCGTATCCGTTCAGGAGAGTGGTTTAAGAAACAATAGATAGGAGATTTTGGAAAGGATTGAGACCCCTTTGTTTCCAA
GTTAAGTATAATGAAATTAT

Product: tRNA nucleotidyltransferase/poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MQVDQETSLIIDAIEEFGGEARLVGGCVRDSILQRDVHDIDLATNLLPNQTIKALKLRNIKTIPTGLNHGTITAILNQRS
FEITTLRHDVKCDGRHAKVEFTNNWQADASRRDFTFNALYADKHGHIYDYFGGIEDLKVRRLNFIGNAEDRIKEDYLRIL
RAFRFHAKICVGDLSDEILNVCKKHSHMIQNLSGERIRDEILKLLECNDPFPTLKSMQESDVLQKIIPKEVKCEILSSSL
LFGTDALVKLALLLRTTKNDRLSLGEYVSKFLRLSNKQKKKLLFLLSNDIKTELSEKEQKKYISLFGRELYCDLVKICGV
ESGENVDEYISFANTFNIPKFPLSGDDLISIGHQPGKSLGRNLELLKQHWEDSSYTLTKEELVLYAKSLF

Sequences:

>Translated_390_residues
MQVDQETSLIIDAIEEFGGEARLVGGCVRDSILQRDVHDIDLATNLLPNQTIKALKLRNIKTIPTGLNHGTITAILNQRS
FEITTLRHDVKCDGRHAKVEFTNNWQADASRRDFTFNALYADKHGHIYDYFGGIEDLKVRRLNFIGNAEDRIKEDYLRIL
RAFRFHAKICVGDLSDEILNVCKKHSHMIQNLSGERIRDEILKLLECNDPFPTLKSMQESDVLQKIIPKEVKCEILSSSL
LFGTDALVKLALLLRTTKNDRLSLGEYVSKFLRLSNKQKKKLLFLLSNDIKTELSEKEQKKYISLFGRELYCDLVKICGV
ESGENVDEYISFANTFNIPKFPLSGDDLISIGHQPGKSLGRNLELLKQHWEDSSYTLTKEELVLYAKSLF
>Mature_390_residues
MQVDQETSLIIDAIEEFGGEARLVGGCVRDSILQRDVHDIDLATNLLPNQTIKALKLRNIKTIPTGLNHGTITAILNQRS
FEITTLRHDVKCDGRHAKVEFTNNWQADASRRDFTFNALYADKHGHIYDYFGGIEDLKVRRLNFIGNAEDRIKEDYLRIL
RAFRFHAKICVGDLSDEILNVCKKHSHMIQNLSGERIRDEILKLLECNDPFPTLKSMQESDVLQKIIPKEVKCEILSSSL
LFGTDALVKLALLLRTTKNDRLSLGEYVSKFLRLSNKQKKKLLFLLSNDIKTELSEKEQKKYISLFGRELYCDLVKICGV
ESGENVDEYISFANTFNIPKFPLSGDDLISIGHQPGKSLGRNLELLKQHWEDSSYTLTKEELVLYAKSLF

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=399, Percent_Identity=36.3408521303258, Blast_Score=206, Evalue=4e-53,
Organism=Escherichia coli, GI87081691, Length=224, Percent_Identity=32.1428571428571, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71995920, Length=383, Percent_Identity=33.9425587467363, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI281360112, Length=397, Percent_Identity=31.7380352644836, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI21357337, Length=397, Percent_Identity=31.7380352644836, Blast_Score=176, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 44580; Mature: 44580

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVDQETSLIIDAIEEFGGEARLVGGCVRDSILQRDVHDIDLATNLLPNQTIKALKLRNI
CCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHCCC
KTIPTGLNHGTITAILNQRSFEITTLRHDVKCDGRHAKVEFTNNWQADASRRDFTFNALY
EECCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCEEEEEEE
ADKHGHIYDYFGGIEDLKVRRLNFIGNAEDRIKEDYLRILRAFRFHAKICVGDLSDEILN
ECCCCCEEHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCKKHSHMIQNLSGERIRDEILKLLECNDPFPTLKSMQESDVLQKIIPKEVKCEILSSSL
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LFGTDALVKLALLLRTTKNDRLSLGEYVSKFLRLSNKQKKKLLFLLSNDIKTELSEKEQK
HHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHEEEEHHCCHHHHHHHHHHH
KYISLFGRELYCDLVKICGVESGENVDEYISFANTFNIPKFPLSGDDLISIGHQPGKSLG
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCHHHC
RNLELLKQHWEDSSYTLTKEELVLYAKSLF
CCHHHHHHHCCCCCCEEEHHHHHHHHHHCC
>Mature Secondary Structure
MQVDQETSLIIDAIEEFGGEARLVGGCVRDSILQRDVHDIDLATNLLPNQTIKALKLRNI
CCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHCCC
KTIPTGLNHGTITAILNQRSFEITTLRHDVKCDGRHAKVEFTNNWQADASRRDFTFNALY
EECCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCEEEEEEE
ADKHGHIYDYFGGIEDLKVRRLNFIGNAEDRIKEDYLRILRAFRFHAKICVGDLSDEILN
ECCCCCEEHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCKKHSHMIQNLSGERIRDEILKLLECNDPFPTLKSMQESDVLQKIIPKEVKCEILSSSL
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LFGTDALVKLALLLRTTKNDRLSLGEYVSKFLRLSNKQKKKLLFLLSNDIKTELSEKEQK
HHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHEEEEHHCCHHHHHHHHHHH
KYISLFGRELYCDLVKICGVESGENVDEYISFANTFNIPKFPLSGDDLISIGHQPGKSLG
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCHHHC
RNLELLKQHWEDSSYTLTKEELVLYAKSLF
CCHHHHHHHCCCCCCEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA