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The map label for this gene is lepA [H]

Identifier: 225630115

GI number: 225630115

Start: 310502

End: 312298

Strand: Reverse

Name: lepA [H]

Synonym: WRi_002950

Alternate gene names: 225630115

Gene position: 312298-310502 (Counterclockwise)

Preceding gene: 225630116

Following gene: 225630114

Centisome position: 21.6

GC content: 37.84

Gene sequence:

>1797_bases
ATGAACAACATAAGGAATTTCGCAATAATAGCGCATATAGACCACGGTAAGTCAACGCTTGCTGACCGTTTAATAGAGGA
ATGTAACGGCCTTGAAGCAAGAGAAATGATCAATCAAATACTCGATTCAATGGATATAGAGCGTGAACGTGGCATTACAA
TTAAAGCACAGACGGTAAAACTTAATTATACGGCAAACGACGGTAATCAATATTGCCTAAATCTCATGGACACCCCAGGT
CACGTTGACTTTTCTTACGAGGTAAGTCGAAGCTTAGCCGCATGTGAAGGTTCACTTTTAGTGGTAGATAGTAGCCAGGG
CGTTGAAGCACAAACCCTTGCAAATGTATATAAAGCTATTGACAACAACCATGAAATAATAGTTGTGCTCAATAAAGTTG
ATCTTCCTGCTGCAGATCCAGAAAAGGTAAAACTCCAGGTTGAGGAAGTAATTGGCATTGACGCAAGTGAGTCAGTTTTA
ATATCAGCAAAAACTGGACTTGGAATAAAAGATGTACTGGAAGCAATAGTAGCAAAACTTCCAGCTCCTCAAGGTGATGT
AAATGCTCCATTGCAAGCAATTTTGGTTGATAGTTGGTATGACACTTACTTAGGAGTAGTAATTTTAGTGCGAGTTAAAA
ATGGAGTGCTAAAAAAAGGCATGAAAATTGTTATGATGTCTAATAATGCTACATATCAGATAGATAATATCGGTATTTTC
ACCCCTAAAAAAGTGATGACGGGTGAACTTTCAGCGGGTGAAGTTGGTTTTATAACTGCTTCAATGAAGGAAGTAGCAGA
CTGCAAGGTAGGAGACACTATCACTGAGGAAAAGAGACCTTGTAGTGAAGCACTTCCTGGATTTAAAGAAGTGCATCCTG
TGGTATTTTGTAGCATTTTTCCTCATAAAACGGATGATTTTAAATATCTAAGAGAAGCTCTAGAAAAGTTACATTTAAAT
GATGCAAGTTTTACCTTCGAAGCTGAAACTTCAAATGCACTAGGCTATGGATTTCGTTGTGGTTTTTTGGGAATGTTGCA
TCTTGAAGTTATTCAAGAAAGGCTCGAGAGAGAATTTGATTTAGACCTAACAGCAACTGCACCGAGTGTTATATATAGGG
TTACAACACGAAGTGGTGAAATTTTGAATATTCATAACCCAAGTGATATGCCAGATCCAACGAAAATTGAAATTGTGGAA
GAACCATGGATTACCGCAACCATAATGGTCCCTGACCAATACTTGGGAGAGATTCTATCTCTATGTGAAGAAAGGAGGGG
AGAACAGGAGGATTTATCTTACATTGGTAACACAACAACAGCATTGTTAAGATATAAATTACCACTGTCTGAAGTTGTTT
TTGATTTTTACGATAGGCTAAAATCAATTTCCAAAGGATATGCAAGCTTAGATTGGGAAATTTCCAGCTATCTGGTAAGC
CAAATAGATAAATTAAGCTTTTTAATTAATGGAGAGCCCGTAGATGCACTGGCTTGTATCGTTCACAAAAGTAGGGCAGA
AAAAAGAGGGCGTGAAATATGCGCACGCTTGAAGGATTTAATACCACGTCAGCAATATAAAATCGCGATTCAAGCGGCAG
TGGGCGGAAAAATTATTGCCAGAGAAACGATTAACCCATACAGAAAAGATGTAACAGCTAAACTCTATGGTGGAGATGTA
ACGCGAAGAATGAAACTACTTGAAAAGCAAAAGAAAGGTAAAAAAAGGTTACATTCTATAGGGAACGTAAATATTCCACA
GAATGCTTTTATTCAAGCCTTGAAGATAAGTGATTGA

Upstream 100 bases:

>100_bases
TCGTCTATGTTGAAGGAGTAAATAGTCTCTTAATAAAGGATTGCCCTCACAACACTTAAAATGTAAAATAGTTTCACTAA
TTAAGTATAGTGGGCCATAA

Downstream 100 bases:

>100_bases
TCTTAAAAGACTTCTTGTATAGTTTTTGGCAACAACCGTTAGAAACGAAAAAACTTACTTGACAAACTCCGCCAGCCCCC
TTATCATGAAACTGAAGCTA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MNNIRNFAIIAHIDHGKSTLADRLIEECNGLEAREMINQILDSMDIERERGITIKAQTVKLNYTANDGNQYCLNLMDTPG
HVDFSYEVSRSLAACEGSLLVVDSSQGVEAQTLANVYKAIDNNHEIIVVLNKVDLPAADPEKVKLQVEEVIGIDASESVL
ISAKTGLGIKDVLEAIVAKLPAPQGDVNAPLQAILVDSWYDTYLGVVILVRVKNGVLKKGMKIVMMSNNATYQIDNIGIF
TPKKVMTGELSAGEVGFITASMKEVADCKVGDTITEEKRPCSEALPGFKEVHPVVFCSIFPHKTDDFKYLREALEKLHLN
DASFTFEAETSNALGYGFRCGFLGMLHLEVIQERLEREFDLDLTATAPSVIYRVTTRSGEILNIHNPSDMPDPTKIEIVE
EPWITATIMVPDQYLGEILSLCEERRGEQEDLSYIGNTTTALLRYKLPLSEVVFDFYDRLKSISKGYASLDWEISSYLVS
QIDKLSFLINGEPVDALACIVHKSRAEKRGREICARLKDLIPRQQYKIAIQAAVGGKIIARETINPYRKDVTAKLYGGDV
TRRMKLLEKQKKGKKRLHSIGNVNIPQNAFIQALKISD

Sequences:

>Translated_598_residues
MNNIRNFAIIAHIDHGKSTLADRLIEECNGLEAREMINQILDSMDIERERGITIKAQTVKLNYTANDGNQYCLNLMDTPG
HVDFSYEVSRSLAACEGSLLVVDSSQGVEAQTLANVYKAIDNNHEIIVVLNKVDLPAADPEKVKLQVEEVIGIDASESVL
ISAKTGLGIKDVLEAIVAKLPAPQGDVNAPLQAILVDSWYDTYLGVVILVRVKNGVLKKGMKIVMMSNNATYQIDNIGIF
TPKKVMTGELSAGEVGFITASMKEVADCKVGDTITEEKRPCSEALPGFKEVHPVVFCSIFPHKTDDFKYLREALEKLHLN
DASFTFEAETSNALGYGFRCGFLGMLHLEVIQERLEREFDLDLTATAPSVIYRVTTRSGEILNIHNPSDMPDPTKIEIVE
EPWITATIMVPDQYLGEILSLCEERRGEQEDLSYIGNTTTALLRYKLPLSEVVFDFYDRLKSISKGYASLDWEISSYLVS
QIDKLSFLINGEPVDALACIVHKSRAEKRGREICARLKDLIPRQQYKIAIQAAVGGKIIARETINPYRKDVTAKLYGGDV
TRRMKLLEKQKKGKKRLHSIGNVNIPQNAFIQALKISD
>Mature_598_residues
MNNIRNFAIIAHIDHGKSTLADRLIEECNGLEAREMINQILDSMDIERERGITIKAQTVKLNYTANDGNQYCLNLMDTPG
HVDFSYEVSRSLAACEGSLLVVDSSQGVEAQTLANVYKAIDNNHEIIVVLNKVDLPAADPEKVKLQVEEVIGIDASESVL
ISAKTGLGIKDVLEAIVAKLPAPQGDVNAPLQAILVDSWYDTYLGVVILVRVKNGVLKKGMKIVMMSNNATYQIDNIGIF
TPKKVMTGELSAGEVGFITASMKEVADCKVGDTITEEKRPCSEALPGFKEVHPVVFCSIFPHKTDDFKYLREALEKLHLN
DASFTFEAETSNALGYGFRCGFLGMLHLEVIQERLEREFDLDLTATAPSVIYRVTTRSGEILNIHNPSDMPDPTKIEIVE
EPWITATIMVPDQYLGEILSLCEERRGEQEDLSYIGNTTTALLRYKLPLSEVVFDFYDRLKSISKGYASLDWEISSYLVS
QIDKLSFLINGEPVDALACIVHKSRAEKRGREICARLKDLIPRQQYKIAIQAAVGGKIIARETINPYRKDVTAKLYGGDV
TRRMKLLEKQKKGKKRLHSIGNVNIPQNAFIQALKISD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=603, Percent_Identity=47.5953565505804, Blast_Score=598, Evalue=1e-171,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=44.0298507462687, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=39.5833333333333, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI25306283, Length=178, Percent_Identity=38.2022471910112, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI19923640, Length=178, Percent_Identity=38.2022471910112, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI25306287, Length=178, Percent_Identity=38.2022471910112, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=43.2432432432432, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=43.2432432432432, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=43.2432432432432, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI18390331, Length=183, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI217272894, Length=178, Percent_Identity=34.2696629213483, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI217272892, Length=161, Percent_Identity=36.0248447204969, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI53729339, Length=224, Percent_Identity=29.9107142857143, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI53729337, Length=224, Percent_Identity=29.9107142857143, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI34147630, Length=257, Percent_Identity=27.2373540856031, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI94966752, Length=97, Percent_Identity=36.0824742268041, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=56.8791946308725, Blast_Score=697, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=506, Percent_Identity=30.4347826086957, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1790835, Length=159, Percent_Identity=33.9622641509434, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1789738, Length=161, Percent_Identity=34.1614906832298, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789559, Length=224, Percent_Identity=29.9107142857143, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=613, Percent_Identity=40.7830342577488, Blast_Score=481, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17556745, Length=157, Percent_Identity=35.6687898089172, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=145, Percent_Identity=35.1724137931034, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=37.3134328358209, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=37.3134328358209, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=221, Percent_Identity=32.1266968325792, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=171, Percent_Identity=32.7485380116959, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32566303, Length=309, Percent_Identity=24.2718446601942, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71994658, Length=232, Percent_Identity=26.7241379310345, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=249, Percent_Identity=28.1124497991968, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=45.1827242524917, Blast_Score=538, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6324707, Length=147, Percent_Identity=40.1360544217687, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=147, Percent_Identity=40.1360544217687, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323098, Length=183, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=138, Percent_Identity=35.5072463768116, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324166, Length=142, Percent_Identity=35.9154929577465, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=322, Percent_Identity=25.4658385093168, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI78706572, Length=603, Percent_Identity=43.7810945273632, Blast_Score=526, Evalue=1e-149,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=42.4460431654676, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24582462, Length=160, Percent_Identity=36.25, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585709, Length=150, Percent_Identity=36.6666666666667, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24585711, Length=150, Percent_Identity=36.6666666666667, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24585713, Length=150, Percent_Identity=36.6666666666667, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI221458488, Length=144, Percent_Identity=39.5833333333333, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21357743, Length=162, Percent_Identity=35.8024691358025, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28572034, Length=240, Percent_Identity=28.75, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19921738, Length=256, Percent_Identity=26.171875, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281363316, Length=287, Percent_Identity=25.4355400696864, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17864358, Length=287, Percent_Identity=25.4355400696864, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66557; Mature: 66557

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIRNFAIIAHIDHGKSTLADRLIEECNGLEAREMINQILDSMDIERERGITIKAQTVK
CCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LNYTANDGNQYCLNLMDTPGHVDFSYEVSRSLAACEGSLLVVDSSQGVEAQTLANVYKAI
EEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
DNNHEIIVVLNKVDLPAADPEKVKLQVEEVIGIDASESVLISAKTGLGIKDVLEAIVAKL
CCCCEEEEEEEECCCCCCCCHHHEEEEHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHC
PAPQGDVNAPLQAILVDSWYDTYLGVVILVRVKNGVLKKGMKIVMMSNNATYQIDNIGIF
CCCCCCCCCCEEEEEEHHHHHHHHHHHEEEEHHCCHHHCCCEEEEEECCCEEEECCEEEE
TPKKVMTGELSAGEVGFITASMKEVADCKVGDTITEEKRPCSEALPGFKEVHPVVFCSIF
CCCHHEECCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHCCCEEEEEEC
PHKTDDFKYLREALEKLHLNDASFTFEAETSNALGYGFRCGFLGMLHLEVIQERLEREFD
CCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
LDLTATAPSVIYRVTTRSGEILNIHNPSDMPDPTKIEIVEEPWITATIMVPDQYLGEILS
CEEEECCCEEEEEEEECCCCEEEECCCCCCCCCCEEEEEECCCEEEEEECCHHHHHHHHH
LCEERRGEQEDLSYIGNTTTALLRYKLPLSEVVFDFYDRLKSISKGYASLDWEISSYLVS
HHHHHCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHH
QIDKLSFLINGEPVDALACIVHKSRAEKRGREICARLKDLIPRQQYKIAIQAAVGGKIIA
HHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEE
RETINPYRKDVTAKLYGGDVTRRMKLLEKQKKGKKRLHSIGNVNIPQNAFIQALKISD
ECCCCHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEECCC
>Mature Secondary Structure
MNNIRNFAIIAHIDHGKSTLADRLIEECNGLEAREMINQILDSMDIERERGITIKAQTVK
CCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LNYTANDGNQYCLNLMDTPGHVDFSYEVSRSLAACEGSLLVVDSSQGVEAQTLANVYKAI
EEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
DNNHEIIVVLNKVDLPAADPEKVKLQVEEVIGIDASESVLISAKTGLGIKDVLEAIVAKL
CCCCEEEEEEEECCCCCCCCHHHEEEEHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHC
PAPQGDVNAPLQAILVDSWYDTYLGVVILVRVKNGVLKKGMKIVMMSNNATYQIDNIGIF
CCCCCCCCCCEEEEEEHHHHHHHHHHHEEEEHHCCHHHCCCEEEEEECCCEEEECCEEEE
TPKKVMTGELSAGEVGFITASMKEVADCKVGDTITEEKRPCSEALPGFKEVHPVVFCSIF
CCCHHEECCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHCCCEEEEEEC
PHKTDDFKYLREALEKLHLNDASFTFEAETSNALGYGFRCGFLGMLHLEVIQERLEREFD
CCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
LDLTATAPSVIYRVTTRSGEILNIHNPSDMPDPTKIEIVEEPWITATIMVPDQYLGEILS
CEEEECCCEEEEEEEECCCCEEEECCCCCCCCCCEEEEEECCCEEEEEECCHHHHHHHHH
LCEERRGEQEDLSYIGNTTTALLRYKLPLSEVVFDFYDRLKSISKGYASLDWEISSYLVS
HHHHHCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHH
QIDKLSFLINGEPVDALACIVHKSRAEKRGREICARLKDLIPRQQYKIAIQAAVGGKIIA
HHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEE
RETINPYRKDVTAKLYGGDVTRRMKLLEKQKKGKKRLHSIGNVNIPQNAFIQALKISD
ECCCCHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA