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The map label for this gene is proP [H]

Identifier: 225630109

GI number: 225630109

Start: 303166

End: 304371

Strand: Reverse

Name: proP [H]

Synonym: WRi_002890

Alternate gene names: 225630109

Gene position: 304371-303166 (Counterclockwise)

Preceding gene: 225630113

Following gene: 225630108

Centisome position: 21.05

GC content: 36.4

Gene sequence:

>1206_bases
ATGCTCTTTATCGATCTGGTTAACATAATTAGTAGAGAGTTTTGTTGTGCAAAAGATGTTTACAACAGAATACTGCAATT
GTTTGGAATTGCAGGACTGGGTGCTATGGTAAGGCCACTTGGCGCATTCATATTTGGTCACATTGGTGACAGGTATGGAA
GGAGAATGGCATTAACGATTGCTATCTTGCTAATATCGATTCCGTCTAGTCTCGTTGCGTTTATTCCAAGTTATAGCAAA
ATAGGTATAACTTCTACTATATTGCTTCTTGCAATCCATGTGACACAAGGGATTGCGCTTGGCGCTGAACAAGGAGGCAG
TTCTGTTTATCTCATAGAGCATTTACCTAATAAGAAAAAACTAGGAATGTTTTTTGGAATAATAAGTTTTGGTCGCTCCA
TTGGCATCTTGCTCTCTGTGATTGTAGTGATCATCTGCAAAAAAAACACTGATTTTAACGCTTGGGGTTGGAGGTTGCCG
TTTACTTTTTCATTTGTTTTGGGGTTAATCAGCGCATATAGTGTATACACATTAGGAGAAACTCCAGCATATGAAGAAAA
TCGAAAACAAAGGAATTTATCTGATTTGCCAATAATAGAGCTTATAAAACGCTACAAGAGAGCTCTGATACTTGCTATTT
TAATATCTGTACCTGTTAATGTTGTTGTTGGATTTACCATATTTCTTCGAACAATTGCAAAAGAAATAGTGTCGGTTGAG
ACGTATGTAACAACATATGTCAATGAAATTGTGCTCATTATAACCAGTATATTAATGCCGGTATCTTCAATAGCGCTCGG
AATGTTAGCTGATAAAGTAGGAAAAGAGCGCACTACAATCTTATTTACAGTGATCACAATGGTACTGTGTTGTCCTGTGT
TATCTACTGCATACTACTATAAAAGTTATCTTATAATTATGCTTGGTGTAATGGCTCTTTCTATAATAGAAAGGGGTATA
AATCCTATAGGAATAGTTGCTGCTGAACTTTTTCCTACCAATGTTAGATTTAGTGGAGTGAGTTTATCACGTAACATCTC
TTATGCGTTACATGGAGGATTTACTCCTATGATATGCACTTGGTTTACTATAACATTTCCTCAAGTAAACTTTGCCGCTG
GGCTTTATGTAGTCTTTTGCTTATTGGTCAGCCTGGTAGCAATATTGCAAATAAAACCGCAAGATAAGAAATTTGATTGG
TCTTAG

Upstream 100 bases:

>100_bases
AGCCACAATGAAATAAGCAAACATTTACAAATTAAATTTAGTGCAAACAAGGGTGTTGATATCAGCTATTCTCTGCAGAA
TCGCAATATGGTATGACCAT

Downstream 100 bases:

>100_bases
TTTTCTCTTAAGTGTGTATTCTCCTAAGTTGAAAAAAGGTGTGAAAAATGCGCTAATCTCGCTAAATGAGAAAATTTTAT
TGCGGAAAAATTAGAACGAT

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MLFIDLVNIISREFCCAKDVYNRILQLFGIAGLGAMVRPLGAFIFGHIGDRYGRRMALTIAILLISIPSSLVAFIPSYSK
IGITSTILLLAIHVTQGIALGAEQGGSSVYLIEHLPNKKKLGMFFGIISFGRSIGILLSVIVVIICKKNTDFNAWGWRLP
FTFSFVLGLISAYSVYTLGETPAYEENRKQRNLSDLPIIELIKRYKRALILAILISVPVNVVVGFTIFLRTIAKEIVSVE
TYVTTYVNEIVLIITSILMPVSSIALGMLADKVGKERTTILFTVITMVLCCPVLSTAYYYKSYLIIMLGVMALSIIERGI
NPIGIVAAELFPTNVRFSGVSLSRNISYALHGGFTPMICTWFTITFPQVNFAAGLYVVFCLLVSLVAILQIKPQDKKFDW
S

Sequences:

>Translated_401_residues
MLFIDLVNIISREFCCAKDVYNRILQLFGIAGLGAMVRPLGAFIFGHIGDRYGRRMALTIAILLISIPSSLVAFIPSYSK
IGITSTILLLAIHVTQGIALGAEQGGSSVYLIEHLPNKKKLGMFFGIISFGRSIGILLSVIVVIICKKNTDFNAWGWRLP
FTFSFVLGLISAYSVYTLGETPAYEENRKQRNLSDLPIIELIKRYKRALILAILISVPVNVVVGFTIFLRTIAKEIVSVE
TYVTTYVNEIVLIITSILMPVSSIALGMLADKVGKERTTILFTVITMVLCCPVLSTAYYYKSYLIIMLGVMALSIIERGI
NPIGIVAAELFPTNVRFSGVSLSRNISYALHGGFTPMICTWFTITFPQVNFAAGLYVVFCLLVSLVAILQIKPQDKKFDW
S
>Mature_401_residues
MLFIDLVNIISREFCCAKDVYNRILQLFGIAGLGAMVRPLGAFIFGHIGDRYGRRMALTIAILLISIPSSLVAFIPSYSK
IGITSTILLLAIHVTQGIALGAEQGGSSVYLIEHLPNKKKLGMFFGIISFGRSIGILLSVIVVIICKKNTDFNAWGWRLP
FTFSFVLGLISAYSVYTLGETPAYEENRKQRNLSDLPIIELIKRYKRALILAILISVPVNVVVGFTIFLRTIAKEIVSVE
TYVTTYVNEIVLIITSILMPVSSIALGMLADKVGKERTTILFTVITMVLCCPVLSTAYYYKSYLIIMLGVMALSIIERGI
NPIGIVAAELFPTNVRFSGVSLSRNISYALHGGFTPMICTWFTITFPQVNFAAGLYVVFCLLVSLVAILQIKPQDKKFDW
S

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=343, Percent_Identity=31.1953352769679, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788292, Length=398, Percent_Identity=27.1356783919598, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789941, Length=393, Percent_Identity=26.7175572519084, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1788942, Length=341, Percent_Identity=26.3929618768328, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 44313; Mature: 44313

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFIDLVNIISREFCCAKDVYNRILQLFGIAGLGAMVRPLGAFIFGHIGDRYGRRMALTI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
AILLISIPSSLVAFIPSYSKIGITSTILLLAIHVTQGIALGAEQGGSSVYLIEHLPNKKK
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEECCCCCCCEEEEEECCCCCHH
LGMFFGIISFGRSIGILLSVIVVIICKKNTDFNAWGWRLPFTFSFVLGLISAYSVYTLGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCC
TPAYEENRKQRNLSDLPIIELIKRYKRALILAILISVPVNVVVGFTIFLRTIAKEIVSVE
CCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYVTTYVNEIVLIITSILMPVSSIALGMLADKVGKERTTILFTVITMVLCCPVLSTAYYY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KSYLIIMLGVMALSIIERGINPIGIVAAELFPTNVRFSGVSLSRNISYALHGGFTPMICT
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEECEEECCCCEEEEECCCHHHHHH
WFTITFPQVNFAAGLYVVFCLLVSLVAILQIKPQDKKFDWS
HHHEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLFIDLVNIISREFCCAKDVYNRILQLFGIAGLGAMVRPLGAFIFGHIGDRYGRRMALTI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
AILLISIPSSLVAFIPSYSKIGITSTILLLAIHVTQGIALGAEQGGSSVYLIEHLPNKKK
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEECCCCCCCEEEEEECCCCCHH
LGMFFGIISFGRSIGILLSVIVVIICKKNTDFNAWGWRLPFTFSFVLGLISAYSVYTLGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCC
TPAYEENRKQRNLSDLPIIELIKRYKRALILAILISVPVNVVVGFTIFLRTIAKEIVSVE
CCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYVTTYVNEIVLIITSILMPVSSIALGMLADKVGKERTTILFTVITMVLCCPVLSTAYYY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KSYLIIMLGVMALSIIERGINPIGIVAAELFPTNVRFSGVSLSRNISYALHGGFTPMICT
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEECEEECCCCEEEEECCCHHHHHH
WFTITFPQVNFAAGLYVVFCLLVSLVAILQIKPQDKKFDWS
HHHEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]