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The map label for this gene is pyrF [H]

Identifier: 225630098

GI number: 225630098

Start: 287936

End: 288610

Strand: Reverse

Name: pyrF [H]

Synonym: WRi_002770

Alternate gene names: 225630098

Gene position: 288610-287936 (Counterclockwise)

Preceding gene: 225630099

Following gene: 225630097

Centisome position: 19.96

GC content: 38.67

Gene sequence:

>675_bases
ATGAACCCAATAATATGCGCACTGGATACACAAGACTTAAATAAGGCTATTTCTCTAGCTAATGCTCTGCGTGGTAAAGT
TGGCATGGTAAAGCTGGGATTAGAATTTTTTGCTGCTCAAGGTCTTTCTGGAGTGCAAGAAGTTGCAAAATGCAATGTAC
CAATTTTTTTAGATCTGAAATTGCATGACATTCCAAACACTGTAGCCAAAACAGTTGAAGTAATAAAAGTTCTGAACATT
GAAATGTTAACTCTGCACATCAGCGGTGGAACAAAAATGCTTGAAGAAGCGCTAAGTGTAGTGCAAGGCACAAAAATAAA
GCTGATTGGAGTGACAGTGCTAACTAGTATGAGCAATGAGGATTTAAACGAGCTTGGAGTAGCAAGAGAGGCAAAATCAC
AGGTAATTTTGCTTGCAAAGCTTGCAAAAAAGATTGGACTCCATGGAGTAGTCTGTTCTGCACTAGAAGCTCAAGAAGTG
CGCCGAGAATGCGGTGAAGACTTTAAAATTATTACTCCAGGAATTCGTATAGATCCAGGTCATGACGACCAAAAAAGAAC
AGCAACACCAAAAGAAGCAATAAATTCAGGAGCTGATTATATCGTCATTGGCAGACCCATTACAAAAACTACAAGTAGTG
CGGAGCTAATATTGAAATCCATGAAAGAATTTTAA

Upstream 100 bases:

>100_bases
ACTATTGACAGAATCTTATGAACGCAGTCAGTTCAATCAGAGTGAAAGTATTTGCAAAACACAACAATGAGTCTTATTAT
AAGATAAATCATGCAAGATA

Downstream 100 bases:

>100_bases
TATAATAACTTAATTTATAAAAATACTAACATATTAATTAGGAATAAGCATGAGTAATATCAATAGAATAATACGCGAAG
TAATCAAGGAATTCCGCTCA

Product: orotidine 5`-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MNPIICALDTQDLNKAISLANALRGKVGMVKLGLEFFAAQGLSGVQEVAKCNVPIFLDLKLHDIPNTVAKTVEVIKVLNI
EMLTLHISGGTKMLEEALSVVQGTKIKLIGVTVLTSMSNEDLNELGVAREAKSQVILLAKLAKKIGLHGVVCSALEAQEV
RRECGEDFKIITPGIRIDPGHDDQKRTATPKEAINSGADYIVIGRPITKTTSSAELILKSMKEF

Sequences:

>Translated_224_residues
MNPIICALDTQDLNKAISLANALRGKVGMVKLGLEFFAAQGLSGVQEVAKCNVPIFLDLKLHDIPNTVAKTVEVIKVLNI
EMLTLHISGGTKMLEEALSVVQGTKIKLIGVTVLTSMSNEDLNELGVAREAKSQVILLAKLAKKIGLHGVVCSALEAQEV
RRECGEDFKIITPGIRIDPGHDDQKRTATPKEAINSGADYIVIGRPITKTTSSAELILKSMKEF
>Mature_224_residues
MNPIICALDTQDLNKAISLANALRGKVGMVKLGLEFFAAQGLSGVQEVAKCNVPIFLDLKLHDIPNTVAKTVEVIKVLNI
EMLTLHISGGTKMLEEALSVVQGTKIKLIGVTVLTSMSNEDLNELGVAREAKSQVILLAKLAKKIGLHGVVCSALEAQEV
RRECGEDFKIITPGIRIDPGHDDQKRTATPKEAINSGADYIVIGRPITKTTSSAELILKSMKEF

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=220, Percent_Identity=39.5454545454545, Blast_Score=154, Evalue=4e-39,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 24126; Mature: 24126

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIICALDTQDLNKAISLANALRGKVGMVKLGLEFFAAQGLSGVQEVAKCNVPIFLDLK
CCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEE
LHDIPNTVAKTVEVIKVLNIEMLTLHISGGTKMLEEALSVVQGTKIKLIGVTVLTSMSNE
ECCCCHHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCC
DLNELGVAREAKSQVILLAKLAKKIGLHGVVCSALEAQEVRRECGEDFKIITPGIRIDPG
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEECCCEEECCC
HDDQKRTATPKEAINSGADYIVIGRPITKTTSSAELILKSMKEF
CCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNPIICALDTQDLNKAISLANALRGKVGMVKLGLEFFAAQGLSGVQEVAKCNVPIFLDLK
CCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEE
LHDIPNTVAKTVEVIKVLNIEMLTLHISGGTKMLEEALSVVQGTKIKLIGVTVLTSMSNE
ECCCCHHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCC
DLNELGVAREAKSQVILLAKLAKKIGLHGVVCSALEAQEVRRECGEDFKIITPGIRIDPG
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEECCCEEECCC
HDDQKRTATPKEAINSGADYIVIGRPITKTTSSAELILKSMKEF
CCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA