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The map label for this gene is tme [H]

Identifier: 225630080

GI number: 225630080

Start: 269399

End: 270724

Strand: Reverse

Name: tme [H]

Synonym: WRi_002570

Alternate gene names: 225630080

Gene position: 270724-269399 (Counterclockwise)

Preceding gene: 225630082

Following gene: 225630079

Centisome position: 18.72

GC content: 39.29

Gene sequence:

>1326_bases
ATGATGAACGATGATTTAGATAGTACCACAAAACAAGAGGCACTTAATTATCACAGTAGAGGTGGTAAACCTGGTAAAAT
TTCAATCATGCCAACAAAACCTTTATCTACCCAGTATGATTTGTCACTTGCTTATTCCCCTGGCGTTGCAGCTCCGTGCC
TTGAAATAGCTAAAAATCCTGAGGTTGTTTATGATTATACGGCAAAAAGCAACTGTGTTGCTGTCATTTCAAATGGCACT
GCAGTGCTTGGACTTGGTAATATCGGTCCTCTTGCTGCAAAACCTGTCATGGAAGGCAAAGCTGTTTTATTTAAGCGTTT
TGCTGATATTGATGCAGTTGATATAGAAGTTGGTACAGAAAATATAGAAGATTTTATCAATGCAGTAAGGTACCTTGGGC
CAAGTTGGGGGGGGATAAATTTAGAGGATATAAGATCTCCCGATTGTTTTATAATAGAGAAACGTCTGAATGAACTGATG
GATATTCCAGTGTTCCATGATGACCAGCATGGAACTGCAGTGGTTGTTGCAGCTGGTATAGAAAATGCTCTTGATATTGT
CGGAAAGAAATTAGAGGACGTTAAGATCATTATGAATGGTGCTGGAGCAGCCGGTATTGCATGTTTAGAAATACTAAAGT
CCATGGGCGCTAAAAATATAGTGCTGTGTGATAAGCAAGGAGTAATATACAAAGGTAGAAACGAGGACATGAATGAGTGG
AAGGAAAAATATGCAATTGATACTTCCGAACGTTCTCTACTTGATACAATAAAAGGCGCTGATGTATTCATCGGACTATC
TGCAAAAGATGTGTTAAATGAAGAGATGTTAAAGAGTATGGGCAAAGACCCGATTATTTTTGCTCTTGCCAATCCCGATC
CAGAAGTAAGGCCTGAGTTTGCAAAATCTGTGAGGCCAGATGCAATAATTGCAACTGGTAGGTCAGATTACAACAACCAA
GTCAACAACGTAATGGGTTTTCCTTATATATTTAGAGGGGCACTTGATGTACATGCAACAACAATAAATGATGAAATGAA
AATTGCAGCTGCAGATGCAATAGCAAAGCTTGCCCGTGAGCCAGTACCAGGTGAGATATCTGCAGTCTATGGCGGTCGTA
AAATGGGCTATGGACGTGAATATATAATACCTACTCCATTTGACCCAAGATTAATTTCTATAGTAGCTCCTGCTGTTGCA
AAAGCCGCAGTTGATTCAGGTGTTGCAAGAAAGGCAATACAAGATTGGAGTAAATATGAAAATCAATTAAAATCTCGCCT
TGCTAGTGCACTTAACATGCTGAAATTACTATCTTCACAATGTTAG

Upstream 100 bases:

>100_bases
TACAGAAGTACACCAGAGTTCACAAGCTAATAGTTTACAATTGAACATTTATAAAAACTCCATATACTTAAAAAATACAA
GCTTAAAATATAAGGATGTA

Downstream 100 bases:

>100_bases
TAACTATATAATCAATAATTCAATTGCACCCAGTCGTTTTAGATTTATAATGAAAGTATTGTAATTTAAAGCAAATCAAT
GGTTATTGCAATTCATGAAC

Product: NADP-dependent malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MMNDDLDSTTKQEALNYHSRGGKPGKISIMPTKPLSTQYDLSLAYSPGVAAPCLEIAKNPEVVYDYTAKSNCVAVISNGT
AVLGLGNIGPLAAKPVMEGKAVLFKRFADIDAVDIEVGTENIEDFINAVRYLGPSWGGINLEDIRSPDCFIIEKRLNELM
DIPVFHDDQHGTAVVVAAGIENALDIVGKKLEDVKIIMNGAGAAGIACLEILKSMGAKNIVLCDKQGVIYKGRNEDMNEW
KEKYAIDTSERSLLDTIKGADVFIGLSAKDVLNEEMLKSMGKDPIIFALANPDPEVRPEFAKSVRPDAIIATGRSDYNNQ
VNNVMGFPYIFRGALDVHATTINDEMKIAAADAIAKLAREPVPGEISAVYGGRKMGYGREYIIPTPFDPRLISIVAPAVA
KAAVDSGVARKAIQDWSKYENQLKSRLASALNMLKLLSSQC

Sequences:

>Translated_441_residues
MMNDDLDSTTKQEALNYHSRGGKPGKISIMPTKPLSTQYDLSLAYSPGVAAPCLEIAKNPEVVYDYTAKSNCVAVISNGT
AVLGLGNIGPLAAKPVMEGKAVLFKRFADIDAVDIEVGTENIEDFINAVRYLGPSWGGINLEDIRSPDCFIIEKRLNELM
DIPVFHDDQHGTAVVVAAGIENALDIVGKKLEDVKIIMNGAGAAGIACLEILKSMGAKNIVLCDKQGVIYKGRNEDMNEW
KEKYAIDTSERSLLDTIKGADVFIGLSAKDVLNEEMLKSMGKDPIIFALANPDPEVRPEFAKSVRPDAIIATGRSDYNNQ
VNNVMGFPYIFRGALDVHATTINDEMKIAAADAIAKLAREPVPGEISAVYGGRKMGYGREYIIPTPFDPRLISIVAPAVA
KAAVDSGVARKAIQDWSKYENQLKSRLASALNMLKLLSSQC
>Mature_441_residues
MMNDDLDSTTKQEALNYHSRGGKPGKISIMPTKPLSTQYDLSLAYSPGVAAPCLEIAKNPEVVYDYTAKSNCVAVISNGT
AVLGLGNIGPLAAKPVMEGKAVLFKRFADIDAVDIEVGTENIEDFINAVRYLGPSWGGINLEDIRSPDCFIIEKRLNELM
DIPVFHDDQHGTAVVVAAGIENALDIVGKKLEDVKIIMNGAGAAGIACLEILKSMGAKNIVLCDKQGVIYKGRNEDMNEW
KEKYAIDTSERSLLDTIKGADVFIGLSAKDVLNEEMLKSMGKDPIIFALANPDPEVRPEFAKSVRPDAIIATGRSDYNNQ
VNNVMGFPYIFRGALDVHATTINDEMKIAAADAIAKLAREPVPGEISAVYGGRKMGYGREYIIPTPFDPRLISIVAPAVA
KAAVDSGVARKAIQDWSKYENQLKSRLASALNMLKLLSSQC

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=344, Percent_Identity=29.0697674418605, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI62420882, Length=350, Percent_Identity=27.7142857142857, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI62420880, Length=350, Percent_Identity=27.7142857142857, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI239049447, Length=350, Percent_Identity=27.7142857142857, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788806, Length=438, Percent_Identity=57.3059360730594, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI87081919, Length=337, Percent_Identity=25.8160237388724, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17537199, Length=413, Percent_Identity=25.6658595641646, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322823, Length=271, Percent_Identity=31.3653136531365, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356279, Length=418, Percent_Identity=27.9904306220096, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281362674, Length=418, Percent_Identity=27.9904306220096, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281362672, Length=470, Percent_Identity=26.8085106382979, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78707242, Length=357, Percent_Identity=25.7703081232493, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI78707238, Length=357, Percent_Identity=25.7703081232493, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI78707240, Length=357, Percent_Identity=25.7703081232493, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24646388, Length=350, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24646386, Length=350, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 47726; Mature: 47726

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNDDLDSTTKQEALNYHSRGGKPGKISIMPTKPLSTQYDLSLAYSPGVAAPCLEIAKNP
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHCCCC
EVVYDYTAKSNCVAVISNGTAVLGLGNIGPLAAKPVMEGKAVLFKRFADIDAVDIEVGTE
CEEEEECCCCCEEEEEECCEEEEEECCCCCCCCCCHHCCHHHHHHHHCCCCEEEEEECHH
NIEDFINAVRYLGPSWGGINLEDIRSPDCFIIEKRLNELMDIPVFHDDQHGTAVVVAAGI
HHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEHHHHHHHHCCCCEECCCCCCEEEEECCH
ENALDIVGKKLEDVKIIMNGAGAAGIACLEILKSMGAKNIVLCDKQGVIYKGRNEDMNEW
HHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCHHHH
KEKYAIDTSERSLLDTIKGADVFIGLSAKDVLNEEMLKSMGKDPIIFALANPDPEVRPEF
HHHHCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH
AKSVRPDAIIATGRSDYNNQVNNVMGFPYIFRGALDVHATTINDEMKIAAADAIAKLARE
HHCCCCCEEEEECCCCCCHHHHHCCCCCHHHHCCEEEEEEEECCCHHHHHHHHHHHHHCC
PVPGEISAVYGGRKMGYGREYIIPTPFDPRLISIVAPAVAKAAVDSGVARKAIQDWSKYE
CCCCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQLKSRLASALNMLKLLSSQC
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMNDDLDSTTKQEALNYHSRGGKPGKISIMPTKPLSTQYDLSLAYSPGVAAPCLEIAKNP
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHCCCC
EVVYDYTAKSNCVAVISNGTAVLGLGNIGPLAAKPVMEGKAVLFKRFADIDAVDIEVGTE
CEEEEECCCCCEEEEEECCEEEEEECCCCCCCCCCHHCCHHHHHHHHCCCCEEEEEECHH
NIEDFINAVRYLGPSWGGINLEDIRSPDCFIIEKRLNELMDIPVFHDDQHGTAVVVAAGI
HHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEHHHHHHHHCCCCEECCCCCCEEEEECCH
ENALDIVGKKLEDVKIIMNGAGAAGIACLEILKSMGAKNIVLCDKQGVIYKGRNEDMNEW
HHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCHHHH
KEKYAIDTSERSLLDTIKGADVFIGLSAKDVLNEEMLKSMGKDPIIFALANPDPEVRPEF
HHHHCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH
AKSVRPDAIIATGRSDYNNQVNNVMGFPYIFRGALDVHATTINDEMKIAAADAIAKLARE
HHCCCCCEEEEECCCCCCHHHHHCCCCCHHHHCCEEEEEEEECCCHHHHHHHHHHHHHCC
PVPGEISAVYGGRKMGYGREYIIPTPFDPRLISIVAPAVAKAAVDSGVARKAIQDWSKYE
CCCCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQLKSRLASALNMLKLLSSQC
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]